Back
 OJI  Vol.10 No.1 , March 2020
Sensitivity of a Rapid Mix Test with Combined Synthetic Antigens Derived from Mycobacterium Leprae PGL-1 for Diagnosis and Surveillance of Leprosy
Abstract: Introduction: In the Americas, Brazil contributes 91.63% of the total cases and the state of Pará still has high endemia for leprosy. Objective: To analyze the performance of a rapid test for the diagnosis and epidemiological surveillance of leprosy in endemic areas. Methods: The sample consisted of 70 MB multibacillary leprosy (MB) patients, 63 paucibacillary (PB) patients, and 80 intradomiciliary consanguineous contacts (ICSCO) of patients. A rapid test with a 15-minute reading was applied using two prototypes: prototype 1, double test with trisaccharide antigen (NT-P-BSA) at 1a. line (83.2 ng/test) and disaccharide antigen (ND-O-BSA) at 2a. (83.2 ng/test), both with a flow of 0.08 μL/mm with a 10 μC membrane, anti-IgM conjugate with a flow of 0.040 μL/mm and a Tris-Triton and prototype 2 runner buffer with MIX antigen (trisaccharide + disaccharide) in the same concentrations and conditions of prototype 1. Results: The comparison of the MIX test positivity rate and the disaccharide or trisaccharide doublet test across all samples was statistically significant, demonstrating that the MIX test had higher seropositivity rates compared to the ND-O-BSA or NT-P-BSA. It was demonstrated that the MIX test showed a good performance, with 25.39% of the PB patients negative for the disaccharide and trisaccharide duplet test, but positive for MIX. Conclusions: These data suggest the potential for further optimizing the performance by adding other synthetic antigens to the MIX antigens.
Cite this paper: Silvestre, M. , Lima, M. , Pereira, A. , Bernardo, A. , Diniz, R. , Ribeiro, P. , Silva, E. and Lima, L. (2020) Sensitivity of a Rapid Mix Test with Combined Synthetic Antigens Derived from Mycobacterium Leprae PGL-1 for Diagnosis and Surveillance of Leprosy. Open Journal of Immunology, 10, 1-9. doi: 10.4236/oji.2020.101001.
References

[1]   (2017) World Health Organization: Global Leprosy Update, 2016: Accelerating Reduction of Disease Burden. No. 35, 92, 501-520.
http://www.wpro.who.int/Leprosy/documents/globalleprosystrategy/2016-2020

[2]   de Vigilancia em Saúde, S. (2018) Ministério da Saúde. Boletim Epidemiológico-Hanseníase, 49, 1-12.

[3]   Pontes, L.R.S.K., Barreto, M.L., Evangelista, C.M.N., Rodrigues, L.C., Heukelbach, J. and e Feldmeir, H. (2006) Socieconomic, Environmental, and Behavioural Risk Factors for Leprosy in North-East Brazil: Results of a Case-Control Study. International Journal of Epidemiology, 35, 994-1000.
https://doi.org/10.1093/ije/dyl072

[4]   Lopes, V.A.S. and Rangel, E.M. (2014) Hanseníase e vulnerabilidade Social: Uma análise do perfil sócio-economico de usuários em tratamento irregular. Saúde Debate, 38, 817-829.
https://doi.org/10.5935/0103-1104.20140074

[5]   Moura, R.S., Calado, K.L., Oliveira, M.L.W. and Sékula, S.B. (2008) Sorologia da hanseníase utilizando PGL-1: Revisao sistemática. Revista da Sociedade Brasileira de Medicina Tropical, 41, 11-18.
https://doi.org/10.1590/S0037-86822008000700004

[6]   Sékula, S.B., Smits, H.L., Gussenhoven, G.C., Leeuwen, J.V., Amador, S., Fujiwara, T., et al. (2003) Simple and Fast Lateral Flow Test for Classification of Leprosy Patients and Identification of Contacts with High Risk of Developing Leprosy. Journal of Clinical Microbiology, 41, 1991-1995.
https://doi.org/10.1128/JCM.41.5.1991-1995.2003

[7]   Duthie, M.S., Goto, W., Ireton, G.C., Reece, S.T., Cardoso, L.P.V., Martelli, C.M.T., et al. (2007) Use of Protein Antigens for Early Serological Diagnosis of Leprosy. Clinical and Vaccine Immunology, 14, 1400-1408.
https://doi.org/10.1128/CVI.00299-07

[8]   Japolla, G., Almeida, G.R., Júnior, J.P.C., Bataus, A.M. and e Souza, G.R.L. (2015) Enciclopédia Biosfera, Centro Científico Conhecer. Teste Imunocromatográfico de Fluxo Lateral: Uma ferramenta rápida de diagnóstico, 11, 2366-2382.
https://doi.org/10.18677/Enciclopedia_Biosfera_2015_208

[9]   Prevedelo, F.C. and Mira, M.T. (2007) Hanseníase: Uma doenca genética? Anais Brasileiros de Dermatologia, 82, 451-459.
https://doi.org/10.1590/S0365-05962007000500009

[10]   Mizoguti, D.F., Hungria, E.M., Freitas, A.A., Oliveira, R.M., Cardoso, L.P.V., Costa, M.B., et al. (2015) Multibacillary Leprosy Patients with High and Persistent Serum Antibodies to Leprosy IDRI Diagnostic-1/LID.1: Higher Susceptibility to Develop Type 2 Reactions. Memórias do Instituto Oswaldo Cruz, 11, 914-920.
https://doi.org/10.1590/0074-02760150198

[11]   Pinto, G.A.T., Freitas, L.G., Machado, Y. and Marinho, P.A. (2015) Avaliacao da técnica de imunocromatografia para análise de drogas de abuso no contexto da química forense. Revista Brasileira de Criminalística, 4, 28-37.

[12]   Sékula, S.B., Sarno, E.N., Oskan, L., Koop, S., Wichers, I., Nery, J.A.C., et al. (2000) Use of Ml Dipstick as a Tool to Classify Leprosy Patients. International Journal of Leprosy, 68, 456-463.

[13]   Amaral, L.C., Goulart, J.M.B., Araújo, S., Reis, E.M. and Goulart, L.R. (2008) Amplificacao da PCR para investigacao da infeccao pelo Mycobacterium leprae em amostra de sangue de pacientes e seus contatos domiciliares.
http://www.researchgate.net/publication/228930310,2014

[14]   Ferreira, M.A.A. and Antunes, C.M.F. (2008) Fatores associados à soropositividade do teste Ml Flow em pacientes e contatos de pacientes com hanseníase menores de 18 anos. Revista da Sociedade Brasileira de Medicina Tropical, 41, 60-66.
https://doi.org/10.1590/S0037-86822008000700013

 
 
Top