JEP  Vol.11 No.1 , January 2020
Prevalence of Aflatoxins in Smoked-Dried and Fresh Fish in Zambia
Abstract: The food contamination is a critical public health concern at the global level. The aflatoxins are considerable food contaminants and health menace to a sizable world population. Aflatoxins originate from fungi as their toxic secondary metabolites. This study aimed to probe the contamination level of aflatoxins in smoked-dried fish and the extent of the threat it might pose on human health. The study considered five of the regularly consumed species of smoked-dried fish. The study also considered two species of fresh fish. The investigation was carried out using VICAM Series-4EX Fluorometer. The Buka fish sample had the lowest concentration of 1.3 ppb for total aflatoxins, and Bream fish was analyzed to have the highest as 3.84 ppb. As such, the total aflatoxin concentration in this study was found to be between 1.3 and 3.84 ppb. These concentrations can be considered to be a matter of concern. Prolonged intake of the aflatoxins in this range may result in a health hazard to humans. As expected, there was no trace of aflatoxins detected in fresh fish samples.
Cite this paper: Singh, I. and Nsokolo, E. (2020) Prevalence of Aflatoxins in Smoked-Dried and Fresh Fish in Zambia. Journal of Environmental Protection, 11, 13-21. doi: 10.4236/jep.2020.111002.

[1]   Bezerra da Rocha, M.E., da Chagas Oliveira Freire, F., Feitosa Maia, F.E., FlorindoGuedes, M.I. and Rondina, D. (2014) Mycotoxins and Their Effects on Human and Animal Health. Food Control, 36, 159-165.

[2]   Tassaneeyakul, W., Razzazi-Fazeli, E., Porasuphatana, S. and Bohm, J. (2004) Contamination of Aflatoxins in Herbal Medicinal Products in Thailand. Mycopathologia, 158, 239-244.

[3]   D’Mello, J.P.F. and Macdonald, A.M.C. (1997) Mycotoxins. Animal Feed Science and Technology, 69, 155-166.

[4]   Yaling, W., Tongjie, C., Guojhong, L., Chunsan, Q., Huiyong, D., Meiling, Y., Bert-Andree, Z. and Gerd, S. (2008) Simultaneous Detection of Airborne Aflatoxin, Ochratoxin, and Zearalenone in Poultry House by Immunoaffinity Column and High Performance Liquid Chromatography. Environmental Research, 107, 139-144.

[5]   Averkieva, O. (2008) Mycotoxins in Grains Harvested in 2008: Wheat. Kemin Industries, Inc., Des Moines, IA.

[6]   Giray, B., Girgin, G., Engin, A.B., Aydin, S. and Sahin, G. (2007) Aflatoxin Levels in Wheat Samples Consumed in Some Regions of Turkey. Food Control, 18, 23-29.

[7]   Hussain, I. and Anwar, J. (2008) A Study on Contamination of Aflatoxin M1 in Raw Milk in the Punjab Province of Pakistan. Food Control, 19, 393-395.

[8]   Jard, G., Liboz, T., Mathieu, F., Guyonvarc’h, A. and Librihi, A. (2011) Review of Mycotoxin Reduction in Food and Feed: From Prevention in the Field to Detoxification by Adsorption or Transformation. Food Additives and Contaminants: Part A, 28, 1590-1609.

[9]   Van Egmond, H.P. (2013) Mycotoxins: Risks, Regulations and European Cooperation. Zbornik Matice Srpske Za Prirodne Nauke, 125, 7-20.

[10]   Cao, H., Huang, H., Xu, W., Chen, D., Yu, J., Li, J. and Li, L. (2011) Fecal Metabolome Profiling of Liver Cirrhosis and Hepatocellular Carcinoma Patients by Ultra Performance Liquid Chromatography-Mass Spectrometry. Analytica Chimica Acta, 691, 68-75.

[11]   Bennet, J.W. and Klich, M. (2003) Mycotoxins. Clinical Microbiology Reviews, 16, 497-516.

[12]   Hanssen, E. and Jung, M. (1973) Control of Aflatoxins in the Food Industry. Pure and Applied Chemistry, 35, 239-250.

[13]   Bucci, T.J., Howard, P.C., Tolleson, W.H., Laborde, J.B. and Hansen, D.K. (1998) Renal Effects of Fumonisin Mycotoxins in Animals. Toxicologic Pathology, 26, 160-164.

[14]   Petzinger, E. and Ziegler, K. (2000) Ochratoxin from a Toxicological Perspective. Journal of Veterinary Pharmacology and Therapeutics, 23, 91-98.

[15]   James, B. (2005) Public Awareness of Aflatoxin and Food Quality Control in Benin. International Institute of Tropical Agriculture.

[16]   Shephard, G.S. (2004) Mycotoxins Worldwide: Current Issues in Africa. In: Barug, D., Van Egmond, H., Lopez-Garcia, R., Van Ossenbruggen, T. and Visconti, A., Eds., Meeting the Mycotoxin Menace, Wageningen Academic, Wageningen, 81-88.

[17]   Lewis, L., Onsongo, M., Njapau, H., Schurz-Rogers, H., Luber, G., Kieszak, S., Nyamongo, J., Backer, L., Dahiye, A.M., Misore, A., Decoct, K. and Rubin, C. (2005) Aflatoxin Contamination of Commercial Maize Products during an Outbreak of Acute Aflatoxicosis in Eastern and Central Kenya. Environmental Health Perspectives, 113, 1763-1767.

[18]   Clifford, J.I. and Rees, K.R. (1967) The Interaction of Afiatoxins with Purines and Purine Nucleosides. Biochemical Journal, 103, 467-471.

[19]   Vidal, A., Mengelers, M., Yang, S., De Saeger, S. and De Boevre, M. (2018) Mycotoxin Biomarkers of Exposure: A Comprehensive Review. Comprehensive Reviews in Food Science and Food Safety, 17, 1127-1155.

[20]   Shephard, G.S. (2008) Impact of Mycotoxins on Human Health in Developing Countries. Food Additives & Contaminants: Part A, 25, 146-151.

[21]   Neal, G.E. (1995) Genetic Implications in the Metabolism and Toxicity of Mycotoxins. Toxicology Letters, 82-83, 861-867.

[22]   Van Egmond, H.P. (1989) Aflatoxin M1: Occurrence, Toxicity, Regulation. In: Van Edmond, H.P., Ed., Mycotoxins in Dairy Products, Elsevier Applied Science, London, 11-55.

[23]   Bukola, C. and Adebayo, T. (2008) Mycofloral of Smoke-Dried Fishes Sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences, 4, 346-350.

[24]   Fredrick, S.J., Immaculate, J.K. and Patterson, E.J.K. (2015) Aflatoxins Investigation on Dried Fishes of Tuticorin, South East Coast of India. Journal of Foodborne and Zoonotic Diseases, 3, 49-62.

[25]   Paul, W.K., Juliet, A., Bandyopadhyay, R. and Peter, J.C. (2018) Aflatoxin Contamination of Dried Insects and Fish in Zambia. Journal of Food Protection, 81, 1508-1518.

[26]   Bruce Manning, B. (2010) Southern Regional Aquaculture Center Publication No. 5002. Mycotoxins in Aquaculture Feed.

[27]   Van Egmond, H.P., Schothorst, R.C. and Jonker, M.A. (2007) Regulations Relating to Mycotoxins in Food: Perspectives in a Global and European Context. Analytical and Bioanalytical Chemistry, 389,147-157.

[28]   FAO (2004) Worldwide Regulations for Mycotoxins in Food and Feed in 2003. Food and Agriculture Organization of the United Nations, Food and Nutrition Paper, No. 81, Rome.

[29]   Akinyemi, A.A., Adejola, A.Q., Obasa, S.O. and Ezeri, G.N.O. (2011) Aflatoxins in Smoked-Dried Fish Sold in Abeokuta, Ogun State, South-West Nigeria. Proceedings of the Environmental Management Conference, Federal University of Agriculture, Abeokuta, Nigeria.

[30]   Hell, K., Fandohan, P., Bandyopadhyay, R., Cardwell, K. and Kiewnick, S. (2008) Pre- and Post-Harvest Management of Aflatoxin in Maize in Mycotoxins: Detection Methods, Management, Public Health and Agricultural Trade. CABI Publishing, Wallingford, UK, 210-219.

[31]   Yazdanpanah, H., Mohammadi, T., Abouhossain, G. and Cheraghali, A.M. (2005) Effect of Roasting on Degradation of Aflatoxins in Contaminated Pistachio Nuts. Food and Chemical Toxicology, 43, 1135-1139.

[32]   Elias-Orozco, R., Castellanos-Nava, A., Gaytán-Martínez, M., Figueroa-Cárdenas, J.D. and Loarca-Pina, G. (2002) Comparison of Nixtamalization and Extrusion Processes for a Reduction in Aflatoxin Content. Food Additives & Contaminants, 19, 878-885.