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 JBM  Vol.8 No.1 , January 2020
Screening Donated Blood for Transfusion-Transmissible Cytomegalovirus Infection among Libyans
Abstract: Human cytomegalovirus (HCMV) is a ubiquitous DNA-containing herpesvirus causes severe and fatal diseases in immunocompromised patients and a prevalent cause of virus-associated birth defects. Blood transfusion donated for neonates, pregnant women, and immunocom-promised patients should be adequately screened for evidences of CMV infection prior to use in clinical management. The effective national programmes for quality-assured screening of donated blood have not yet been fully established, hence this study was undertaken to assess whether any bloodborne-CMV infections pose a significant threat to the safety of the blood supplies. A total of 200 voluntary blood donor subjects admitted to the Blood Bank of Benghazi/Libya were screened for transfusion-transmissible CMV (TT-CMV) using a highly sensitive CMV total IgG and IgM antibody enzyme immunoassay as well as CMV pp65 anti-genemia assays. We determined that the overall seropositivity for IgG antibodies (80.50%) was higher than that of IgM antibodies (39.00%), but only 2 (1.00%) individuals out of these donors were seropositive for the CMV-antigenic protein pp65. The frequency of CMV infection based on gender was incomparable due to the small population number of blood-donated females. According to age, there was not influence of various age groups on prevalence of anti-CMV IgG antibodies, while a progressive increase in seropositivity of CMV-IgM antibodies with age was detected. The age groups were not significantly associated with CMV prevalence. In contrast, only 2 (1.00%) patients were shown to be positive for all three performed assays indicating a recurrent infection. Our findings prove a risk of primary transfusion-associated transmission of CMV and may provide a policy guidance on ensuring safe blood supplies accessible to all patients who require transfusion.
Cite this paper: Bleiblo, F. , Eljaki, A. , Bumadian, M. , Elwaheishi, K. , Almismary, E. , Aljlale, M. , Alghazal, R. and Abraheem, M. (2020) Screening Donated Blood for Transfusion-Transmissible Cytomegalovirus Infection among Libyans. Journal of Biosciences and Medicines, 8, 5-12. doi: 10.4236/jbm.2020.81002.
References

[1]   Cannon, M.J., Schmid, D.S. and Hyde, T.B. (2010) Review of Cytomegalovirus Seroprevalence and Demographic Characteristics Associated with Infection. Reviews in Medical Virology, 20, 202-213.
https://doi.org/10.1002/rmv.655

[2]   Mocarski, E.S., Shenk, Griffiths, P.D. and Pass, R.F. (2013) Cytomegaloviruses. In: Knipe, D.M. and Howley, P.M., Eds., Field Virology, 6th Edition, Lippincott Williams & Wilkin, Philadelphia, 1960-2014.

[3]   Brooks, G.F., Carroll, K.C., Butel, J.S., Morse, S.A. and Mietzner, T.A. (2010) Jawetz, Melnick and Adelberg’s Medical Microbiology. 25th Edition, McGraw-Hill Companies Inc., New York, 467-488.

[4]   Trkulic, M., Jovanovic, D., Ostojic, G., Kovacevic, Z. and Taseski, J. (2000) Cytomegalovirus Infection in Patients with Kidney Diseases. Vojnosanitetski Pregled, 57, 63-67.

[5]   Cavlek, T., Kolaric, B., Sternak, S., Kos, M., Kaic, B. and Galinovic, G. (2015) Prevalence and Dynamics of Cytomegalovirus Infection among Patients Undergoing Chronic Hemodialysis. Indian Journal of Nephrology, 25, 95-98.
https://doi.org/10.4103/0971-4065.139488

[6]   van Prooijen, H.C., Visser, J.J., van Oostendorp, W.R., de Gast, G.C. and Verdonck, L.F. (1994) Prevention of Primary Transfusion-Associated Cytomegalovirus Infection in Bone Marrow Transplant Recipients by the Removal of White Cells from Blood Components with High-Affinity Filters. British Journal of Haematology, 87, 144-147.
https://doi.org/10.1111/j.1365-2141.1994.tb04884.x

[7]   Roback, J.D. (2002) CMV and Blood Transfusions. Reviews in Medical Virology, 12, 211-219.
https://doi.org/10.1002/rmv.353

[8]   Kothari, A., Ramachandran, V.G., Gupta, P., Singh, B. and Talwar, V. (2011) Seroprevalence of Cytomegalovirus among Voluntary Blood Donors in Delhi India. Journal of Health, Population and Nutrition, 20, 348-351.

[9]   Pal, S.R., Chitkara, N.L. and Krech, U. (1972) Sero-Epidemiology of Cytomegalovirus Infection in and around Chandigarh (Northern India). Indian Journal of Medical Research, 60, 973-978.

[10]   Madhavan, H.N., Prakash, K. and Agarwal, S.C. (1974) Cytomegalovirus Infection in Pondicherry (South India)—A Serological Survey. Indian Journal of Medical Research, 62, 297-300.

[11]   Mukundan, P., Jadhav, M. and John, T.J. (1977) Prevalence of Cytomegalovirus Antibody in Young Children in Vellore. Indian Journal of Medical Research, 65, 589- 592.

[12]   Furui, Y., Satake, M., Hoshi, Y., Uchida, S., Suzuki, K. and Tadokoro, K. (2013) Cytomegalovirus (CMV) Seroprevalence in Japanese Blood Donors and High Detection Frequency of CMV DNA in Elderly Donors. Transfusion, 53, 2190-2197.
https://doi.org/10.1111/trf.12390

[13]   Bawa, M.K., Mamman, A., Olayinka, A., Gidado, S., Waziri, N.E., Balogun, M.S., Getso, K.I., Dalhat, M.M., Nsubuga, P., Aliyu, N., Bala, H., Muhammad, H., Haladu, S., Shehu, U.L. and Nguku, P.M. (2019) Blood Donor Safety, Prevalence and Associated Factors for Cytomegalovirus Infection among Blood Donors in Minna-Nigeria, 2014. The Pan African Medical Journal, 32, 1-6.
https://doi.org/10.11604/pamj.supp.2019.32.1.13297

[14]   Hecker, M., Qiu, D., Marquardt, K., Bein, G. and Hackstein, H. (2004) Continuous Cytomegalovirus Seroconversion in a Large Group of Healthy Blood Donors. Vox Sanguinis, 86, 41-44.
https://doi.org/10.1111/j.0042-9007.2004.00388.x

[15]   Lancini, D.V., Faddy, H.M., Ismay, S., Chesneau, S., Hogan, C. and Flower, R.L. (2016) Cytomegalovirus in Australian Blood Donors: Seroepidemiologic and Seronegative Red Blood Cell Component Inventories. Transfusion, 56, 1616-1621.
https://doi.org/10.1111/trf.13459

[16]   Pembrey, L., Raynor, P., Griffiths, P., Chaytor, S., Wright, J. and Hall, A.J. (2013) Seroprevalence of Cytomegalovirus, Epstein Barr Virus and Varicella Zoster Virus among Pregnant Women in Bradford: A Cohort Study. PLoS ONE, 8, e81881.
https://doi.org/10.1371/journal.pone.0081881

[17]   Ziemann, M., Unmack, A., Steppat, D., Juhl, D., Gorg, S. and Hennig, H. (2010) The natural Course of Primary Cytomegalovirus Infection in Blood Donors. Vox Sanguinis, 99, 24-33.
https://doi.org/10.1111/j.1423-0410.2009.01306.x

[18]   Bate, S.L., Dollard, S.C. and Cannon, M.J. (2010) Cytomegalovirus Seroprevalence in the United States: The National Health and Nutrition Examination Surveys, 1988-2004. Clinical Infectious Diseases, 50, 1439-1447.
https://doi.org/10.1086/652438

[19]   Staras, S.A., Dollard, S.C. and Radford, K.W. (2006) Sero-prevalence of Cytomegalovirus Infection in the United States, 1988-1994. Clinical Infectious Diseases, 43, 1143-1151.
https://doi.org/10.1086/508173

[20]   Grifiths, P.D. and Baboonian, C.A. (1984) Prospective Study of Primary Cytomegalovirus Infection during Pregnancy: Final Report. BJOG, 91, 307-315.
https://doi.org/10.1111/j.1471-0528.1984.tb05915.x

[21]   Stadler, L.P., Bernstein, D.I. and Callahan, S.T. (2010) Sero-prevalence of Cytomegalovirus (CMV) and Risk Factors for Infection in Adolescent Males. Clinical Infectious Diseases, 51, 76-81.
https://doi.org/10.1086/656918

[22]   Souza, M.A., Passos, A.M., Treitinger, A. and Spada, C. (2010) Seroprevalence of Cytomegalovirus Antibodies in Blood Donors in Southern, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 43, 359-361.
https://doi.org/10.1590/S0037-86822010000400004

 
 
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