Aloe vera is a hardy, perennial, tropical succulent plant with properties like, drought resistant. It belongs to the family Liliaceae which, historically has been used for various medicinal purposes. Genus Aloe contains more than 500 species of flowering succulent plants in the world . The size of these plants varies from an inch to two feet or more in diameter. These plants are highly complex in shape and spread out colonies consisting of thousands of plants. Many Aloe species are scattered naturally in North Africa. Among 500 species, most commonly occurring species of A. vera is Aloe barbadensis .
A. vera is generally cultivated in moderate environments rather than chilling, such as tropical or sub-tropical regions. Another beauty of this plant is blooming; some older plants bloom and produce beautiful flowers with tall stock covered by bright color petals.
Aloe vera, a short-stemmed shrub is described as a “wonder plant”, due to its frequent use in various medical products. The gel obtained from the plant’s leaves has been used in many pharmaceutical products (Figure 1). The medicinal properties are well documented in various Indian indigenous system of medicine like Ayurveda, Siddha, Unani and Homeopathy . Extensive research since last decades has revealed that the pharmacologically active ingredients are distributed in both the gel and rind of the A. vera leaves. A. vera is very popular in cosmetic and pharmaceutical industries  .
Different products from A. vera gel which are commercially available in the market are depicted in Figure 2.
A. vera is very popular in skin care markets due to its cooling effect, which protects skin from long time exposure of sun light  . It helps in eliminating skin diseases like acne and eczema (Figure 3). Aloe is also marketed as a remedy for ulcer, diabetes, cancer, arthritis, cough, wound and immune disorders. It has been proven as an effective antiseptic, powerful detoxifier and tonic for the nervous system .
Aloe flowers are usually tubular or trumpet-shaped and are arranged in clusters and their fruit is capsular. There are more than 20 species of aloe and their number is growing steadily, as new hybrids are constantly being created. The acíbar, a yellowish liquid layer present between the skin of penca and the gel of Aloe vera is composed of aloin. It is bitter in taste and by boiling, it is transformed
Figure 1. Aloe vera plantlets and gel present in mature leaves.
Figure 2. List of products made from A. vera gel.
Figure 3. The pharmacological attributes which can be cured by A. vera gel products.
into a dark brown amorphous mass. Garcia et al. (2019) explained that acibar is composed of 40% to 80% of resin, and up to 20% of aloin, anthraquinone glucoside  . The bark, green or bluish green exhibits 20% to 30% of the total weight of the plant. Color of the bark depends on various factors such as temperature, nutrition, climate and physiology of the plants. Pulp or gel is basically parenchyma tissues located at the central part of the leaf and exhibits 65% to 80% of the total weight  . After fertilization, the flower matures and turns into a small fruit which later releases the seeds into atmosphere through wind. Since A. vera grows in difficult environmental conditions, so, apart from seed dispersal phenomenon it also reproduces by means of stolon or shoots, which are born at the basal part of stem. This new plant grows together with the mother plant and plant density increases with greater biomass    .
2. Chemical Constituents of A. vera
A. vera is a rich source of different chemical compounds which play a very important role in commercial production of medicines. Chemical composition of A. vera revealed the presence of <200 different biologically active molecules including carbohydrates, essential amino acids, enzymes, vitamins, minerals, secondary metabolites like; anthraquinones or phenolic compounds, saponins, sterols, and salicylic acid  . Studies have revealed that there are 75 ingredients present in the Aloe leaf and have many nutraceutical properties. These chemical compounds are divided into following categories:
Anthraquinones: Almost 12 varieties of anthraquinones; Aloin, Isobarbaloin, Anthracene, Emodin, Ester of Cinnamonic acid, Chrysophanic acid, Barbaloin, Anthranol, Aloetic acid, Aloe Emodin, Ethereal oil and Resistannol are present in A. vera sap. They perform the functions of natural laxatives, painkillers and analgesics  . Figure 4 showing various metabolic components present in different A. vera leaves and tissues which are useful for pharmaceutical and beauty products. They contain powerful antibacterial, antifungal and virucidal properties. Two compounds, Aloin and Emodin have analgesic properties, and also work as potent antibacterials and antivirals    .
Enzymes: Following pharmacologically important enzymes are identified from various parts of A. vera plants: bradykinase, peroxidise, lipase, aliase, cellulose, alkaline phosphatase, carboxypeptidase, amylase and catalase. Bradykinase facilitates to lessen the excessive inflammation of skin. Other enzymes help in the breakdown of fats and sugars, which help in fitness of our body by reducing the blood pressure and controls the sugar level in the blood  .
Sugars: A. vera produces many important monosaccharides and polysaccharides. These are derived from the leaf’s mucilaginous layer. One of the most
Figure 4. Metabolic components present in different A. vera leaf tissues.
important monosaccharide is mannose-6-phosphate, and polysaccharide is glucomannans [beta-(1,4)-acetylated mannan]. In recent studies, a glycoprotein with antiallergic properties, known as alprogen and novel anti-inflammatory compound, C-glucosylchromone, has been identified in A. vera gel   .
Essential fatty Acids: There are 4 kinds of plant steroids present in A. vera; lupeol, LDL cholesterol, β-sisosterol and campesterol. All of these have anti-inflammatory properties and helps in wound cure. Another fatty acid constituent lupeol also possesses antiseptic and analgesic properties and is used in various medicinal products  .
Minerals: Essential minerals required for the proper functioning of various physiological and metabolic pathways are significantly present in A. vera. These minerals include; calcium, chromium, copper, selenium, magnesium, manganese, potassium, sodium and zinc. These minerals are essential cofactors of various enzymes which are being used in different metabolic processes  .
Vitamins: Vitamins are essential supplements to neutralize the adverse effect of free radicals and act as antioxidants. Vitamins protect the cells and tissues from toxic effects of these free radicals. A fair amount of Vitamin A (beta-carotene), vitamin C, vitamin E, vitamin B12, folic acid, and choline are identified from various parts of A. vera plantlets  .
Others constituents: Out of 22 amino acids, 20 amino acids and out of 08 essential amino acids (important for human beings), 07 essential amino acids were identified in A. vera plants. Two important plant hormones, Auxins and Gibberellins are present in A. vera which assist in wound recuperation and reduce inflammation. Additionally, salicylic acid holds antibacterial and anti-inflammatory properties. Saponins, which is a major component of A. vera gel (about 3%), possesses cleansing and antiseptic properties . An inert polysaccharide lignin from A. vera is also used in skin medicines to enhance the penetrative effect of the other ingredients inside the skin tissues .
3. Mechanism of Actions
Wound healing: A mannose-rich polysaccharide, Glucomannan and a growth hormone, gibberellin, interacts with growth factor receptors at the fibroblast. Then it stimulates its activity and proliferation, which in turn notably increases collagen synthesis after oral intake. Aloe gel helps in increasing the collagen content on the wound which facilitates the penetration of other constituents inside the skin tissue   . It speeds up the wound contraction and accelerated the process of ensuing scar tissue. An increased synthesis of dermatan sulphate and hyaluronic acid in the granulation tissue of a healing wound accelerated the process of curing the wound  .
Anti-aging effects: Mucopolysaccharides present in skin tissue helps in binding moisture. A. vera sap/gel stimulates fibroblast cells which produces the elastin fibers and collagen. These two makes the skin pores less elastic and makes the skin tight and less wrinkled. Additionally, Aloe has cohesive properties which binds with the superficial epidermal tissue and stick them together and softens the skin. Another important mineral Zinc, an astringent helps to tighten the skin pores. Amino acids help in softening the hardened skin cells and nourish the skin cells. Due to its moisturizing effect A. vera has been extensively studied in the field of treatment of dry skin. It has been proven Aloe gel improved the skin integrity, reduces wrinkles and decreases erythema. Apart from these effects aloe gel also shows anti-acne impact    .
Immunity booster: Calcium influx into mast cells due to inhibition of Alprogen, by inhibiting the antigen-antibody-mediated launch of histamine and leukotriene from mast cells. Anthraquinones stimulates the host’s immune system. These actions are also act as antitumor activity. The anthraquinone and aloin inactivates various enveloped viruses like herpes simplex, varicella zoster and influenza and inhibit the progression inside the host cell  .
Laxative effects: A molecule anthraquinone present in A. vera shows laxative effects. It stimulates mucus secretion by restoring intestinal water content thus increases intestinal peristalsis  .
UV protection: It has been reported that A. vera gel have a defensive effect against radiation damage to the skin  .
Anti-inflammatory properties: A. vera reduces prostaglandin E2 manufacturing from arachidonic acid by inhibiting the cyclooxygenase pathway  .
Antiseptic properties: Six antiseptic agents; Lupeol, salicylic acid, urea nitrogen, cinnamonic acid, phenols and sulfur has been identified in A. vera. These agents show inhibitory effect against microorganisms like fungi, bacteria and viruses  .
4. Piriformospora indica: A Mutualistic Plant Growth Promoter
P. indica is root colonizing endophytic fungus, extensively studied since last two decades due to its unique plant growth promoting properties. It helps plant to acquire more nutrients from soil even under extreme physical and nutrient stress conditions  . It interacts with a wide range of hosts, including bryophytes, pteridophytes, gymnosperms and cruciferiae and large number of mono and dicot plants . The fungus promotes nutrient uptake, allows plants to survive under water, temperature and salt stresses, and confers systemic resistance to toxins, heavy metal ions, insects and pathogenic organisms. Further, it is shown to stimulate excessive production of biomass, early flowering, seed production and a potential microorganism imparting biological hardening to tissue culture-raised plants     (Figure 5).
4.1. P. indica Promotes Growth of Aloe vera with Value Additions
The study had been undertaken to investigate the effect of endophyte P. indica on the physiological growth and the essential phytochemical contents of A. vera. The results showed overall increase in plant biomass and greater shoot and root length as well as number of shoots and roots as compared to control under both in vitro and in vivo environment conditions (Figure 6). Apart from that the photosynthetic pigment (Chl a, Chl b and total Chl) and gel content were
Figure 5. (a) Ultrastructure of P. indica Chlamydospore and (b) Globe showing important properties of P. indica since the year it has been discovered.
Figure 6. Interaction of P. indica with A. vera plantlets under in vitro conditions.
observed significantly higher (93.45%, 101.61%, and 60.46% respectively) in symbiotic fungus inoculated A. vera plantlets  .
The interaction study of medicinal plant with P. indica was conducted at large scale in bio hardening faculty at Amity University Noida, India (Figure 7).
The root colonization study with P. indica showed 67.5% colonization in A. vera roots. The percentage of colonization inside the root of plants showed the affinity of host-symbiont relationship. This colonization resulted in 16.5% increment in gel content along with 100% survival rate of colonized plants. This gel content has a great commercial importance to cosmetics and medicine industries as described earlier in this article. P. indica treated plantlets also had 52.53% higher aloin content as compared to the control plantlets. The aloin content as determined by HPLC was found to be 1.28 ± 0.057 mg aloin/g of leaf exudate for the treated and 0.844 ± 0.001 for the control set, marking an overall increase of 52.23%. This aloin is a potential anticancer agent and used as a laxative. These have various therapeutic properties and are used frequently in recovery from radiation damage and wound healing etc. .
Figure 7. The green house experiments of A. vera plants. Plants have been growing in bio-hardening facility of Amity University.
Further, the colonized host had higher phenolic content (41.73 ± 3.73 mg gallic cid equivalents/g dry wt.) as well as appreciable increase in radical scavenging capacity ranging from 20.05 ± 0.43 to 70.03 ± 0.98 (% antioxidant activity or % DPPH radicals inhibited). This confirms the ability of fungus to reduce oxidative damage associated with many phyto-pathogenic diseases and impart a greater tolerance to plants against pathogens. There could have been a shift in metabolic carbon flow towards antioxidant biosynthesis  .
4.2. P. indica Facilitates Nutrient Supply and Activates Various Biological Pathways
Our laboratory at Amity University, Noida first time successfully performed the interaction study of P. indica with A. vera under in vitro conditions. After successful colonization of P. indica, it facilitates the transfer/supply of nutrients like nitrogen and phosphorus to the roots of host plant which stimulates the cascade of biological reactions to synthesize various proteins, which participated in growth promotion of plant, enhancement of secondary metabolites, boosting immune system to trigger the defense mechanism against various biotic and abiotic stresses to increase the plant fitness  .
P. indica is considered as a potential candidate to enhance the biomass production along with various value additions in the form of active ingredients. It significantly increases the vegetative growth of plant and boost immunity to sustain in diverse environment as compared to control. The pharmaceutically important metabolites were also found increased many folds in fungal treated A. vera host.
The study proves the future prospective of P. indica being used as biopriming agent for achieving better growth, better survival rate along with enhanced growth of in vitro raised plantlets along with substantial enhancement of secondary metabolites. The mechanism involved in interaction with mycobiont with host needs to be elucidated to understand the physiology behind this phenomenon.
Authors are thankful to ICAR-NASF, DST FIST and DST Nano Mission for partial funding.
 Nordqvist, C. (2017) Nine Health Benefits and Medical Uses of Aloe vera.
 Mahor, G. and Ali, S.A. (2016) Recent Update on the Medicinal Properties and Use of Aloe vera in the Treatment of Various Ailments. Bioscience Biotechnology Research Communications, 9, 277-292.
 Rajeswari, R., Umadevi, Sharmila, C., Pushpa, R., Selvavenkadesh, S. and Bhowmik, D. (2012) Aloe vera: The Miracle Plant Its Medicinal and Traditional Uses in India. Journal of Pharmacognosy and Phytochemistry, 1, 118-124.
 Chithra, P., Sajithlal, G.B. and Chandrakasan, G. (1998) Influence of Aloe vera on the Glycosaminoglycans in the Matrix of Healing Dermal Wounds in Rats. Journal of Ethnopharmacology, 59, 179-186.
 Maan, A.A., Nazir, A., Khan, M.K.I., Ahmad, T., Zia, R., Murid, M. and Abrar, M. (2018) The Therapeutic Properties and Applications of Aloe vera: A Review. Journal of Herbal Medicine, 12, 1-10.
 Varma, A., Bakshi, M., Lou, B., Hartmann, A. and Oelmueller, R. (2012a) Piriformospora indica: A Novel Plant Growth-Promoting Mycorrhizal Fungus. Agricultural Research, 1, 117-131.
 Varma, A., Sherameti, I., Tripathi, S., Prasad, R., Das, A., Sharma, M., Bakshi, M., Johnson, J.M., Bhardwaj, S., Arora, M., Rastogi, K., Agrawal, A., Kharkwal, A.C., Talukdar, S., Bagde, U.S., Bisaria, V.S., Upadhyaya, C.P., Won, P.S. and Chen, Y. (2012) The Symbiotic Fungus Piriformospora indica: Review. Mycota, 9, 231-254.
 Su, Z.Z., Wang, T., Shrivastava, N., Chen, Y.Y., Liu, X., Sun, C., Yin, Y., Gao, Q. and Lou, B.G. (2017) Piriformospora indica Promotes Growth, Seed Yield and Quality of Brassica napus L. Microbiological Research, 199, 29-39.
 Tyagi, J., Bandyopadhyay, P., Shrivastava, N., Sharma, A.K., Varma, A. and Pudake, R.N. (2017) Effect of Inoculum Concentrations of Piriformospora indica (Serendipita indica) on Phenotypic and Biochemical Characteristics of Finger Millet Plant under Drought Stress. Biochemical and Cellular Archives, 17, 427-434.
 Sharma, P., Kharkwal, A.C., Abdin, M.Z. and Varma, A. (2014) Piriformospora indica Improves Micropropagation, Growth and Phytochemical Content of Aloe vera L. Plants. Symbiosis, 64, 11-23.
 Shrivastava, N., Jiang, L., Li, P., Sharma, A.K., Luo, X., Wu, S. and Lou, B. (2018) Proteomic Approach to Understand the Molecular Physiology of Symbiotic Interaction between Piriformospora indica and Brassica napus. Scientific Reports, 8, Article No. 5773.