Mount Everest (known also as Sagarmatha and Chomolungma), the highest mountain in the world, is located in the Sagarmatha National Park, Nepal (27˚56'N, 86˚44'E), which extends from 2610 m to 8848 m elevation. Communities in the Sagarmatha National Park and associated Buffer Zone (from here on known as the combined SNPBZ, Figure 1), some of the highest-altitude villages in the world experience poor health outcomes due to contaminated drinking water. Diseases initiated by contaminated water are some of the most common causes of death in developing nations. According to the World Health Organization , unsafe drinking water and inadequate sanitation practices are responsible for more than 80% of diseases globally, and specifically, contaminated drinking water contributes to more than one billion cases of diarrhea each year; yet, over 800 million people lack basic drinking water service . These problems are more prevalent in developing countries where unsafe drinking water contributes to a number of health issues .
Availability and access to clean drinking water in the SNPBZ is a complex challenge, involving environmental degradation, economics, climate change, and governance. The park was established in 1976 with an area of 1148 km2, declared as a World Natural Heritage Site in 1979, and added a protective buffer zone of ~275 km2 in 2002. Community, regional, and national governance within the park is multifaceted. The spectacular scenery attracts trekkers who simultaneously
Figure 1. Location map showing the regional topography and drainage basins, with vegetation in green and snow/ice cover in white, for the SNP and surrounding area. The map also shows the location of Jiri where precipitation data is collected, major villages in the SNP and sampling locations.
drive the local economy as well as contribute significantly to environmental degradation. The region has a local population of about 6000 people, while tourist numbers rose to peak numbers of 36,518 in 2012, not even counting a large number of service personnel such as porters and guides who accompany the tourists  . The refuse generated by tourists within the SNPBZ is causing substantial anthropogenic pressure with serious environmental consequences to the unique ecosystems   . Although the impact is visible primarily along the more popular trekking routes, unmanaged or poorly managed solid waste disposal and open defecation have resulted in contamination of major rivers    and many drinking water sources throughout the region   .
In recent years, local communities have become more aware of and concerned about drinking water safety and water security    . The onus of managing SNP’s drinking water quality, and of managing sewage and waste, has devolved from the national government to citizens and communities across Nepal, including those of the SNPBZ. Healthy ecosystems and community welfare are interdependent and are at the heart of community-based ecosystem management . Unfortunately, both the tourism industry’s unchecked development and climate change are impacting ecosystem health, particularly drinking water quality, and people’s quality of life; further compromising their ability to manage water resources in ways beneficial to the greater community. Hence, understanding a range of factors from household water use through localized hydrology is necessary in order to facilitate community-driven management and sustainability practices that support water quality risk mitigation and improve human health .
Water resources in the SNPBZ are not just important to local Sherpa communities. The Himalayan Mountains are the headwaters for major river systems such as the Ganges, Yangtze and Indus Rivers which provide water resources to approximately 1.4 billion people, hence understanding all aspects of the hydrologic cycle is important. The current and future impact of climate change, particularly glacial melting and changing precipitation patterns  and references therein], will affect all those dependent on Himalayan water resources. The threat to both water and food security in the region is significant, and understanding the impacts within the headwaters of the region must be fully explored in order that communities, regions and countries can act to reduce long-term vulnerability to climate change.
The study area begins in the SNPBZ township of Lukla, follows the main trekking route towards Mt. Everest base camp, and then drops back down to Lukla (Figure 1). Tourist and resident population numbers are highest at the lower elevations. The entire route is characterized by rugged terrain and topography, and ranges in altitude from 2610 m to 5300 m. The SNPBZ has a temperate climate characterized by cold winters, warm summers and clear seasonality, with temperature ranges from 37˚C in summer to −17˚C in winter. Maximum rainfall generally occurs between June and September, coinciding with the Indian Monsoon (Table 1).
Water samples were collected during the pre-monsoon season: late April and early May 2016, 2017 and 2018. Sampling sites are located along the main trekking route between Lukla and Lobuche (Figure 1) and were selected based on access to and availability of the water for local and tourist use as drinking water. Where possible, the actual source (generally springs) of drinking water was sampled. Sample sites include 16 samples of subsurface-fed springs (either directly from the spring or from flowing standpipes (water brought via tubing from springs at higher elevations), and 8 stream samples. Only water currently being used as drinking water was sampled, hence the major rivers are not included in this study. None of the samples were collected from standing or stored water.
Fecal contamination in water sources poses significant health risks; the presence of coliform bacteria and E. coli can be used as an indicator of fecal contamination of water and potential danger to human health. In natural systems, microorganisms are widely distributed and their diversity and abundances may be used as an indicator for suitable water sources . Although there is a wide range of pathogenic microorganisms that can be transmitted to humans via water contaminated with fecal material (see  and references therein), the isolation and identification of these organisms are complicated and seldom quantitative  . It is not practical to test water for all of these organisms, and measurement of coliform bacteria (total coliform bacteria and/or fecal coliforms) can be used as an indirect approach based on the assumption that groups of normal enteric organisms will indicate the level of fecal contamination of the water supply     .
Temperature, pH and conductivity were measured in the field using a FisherSci Ap85 pH/conductivity meter. Samples for bacteria analyses were collected in sterile syringes and 100 mL of sample was passed through a 0.45 µm filter at the sample site. After filtration the filter paper was placed in a sterile test card (manufactured by Micrology Labs®) containing a medium which uses two color-producing chemicals, one for the detection of the enzyme glucuronidase (produced by E. coli strains but not by general coliforms) and one for the detection of galactosidase (produced by all coliforms, including E. coli). The samples were then placed into a portable field incubator and kept as close to 35˚C as possible for 24 hours (for detailed field methods refer to  ). Sample counts were done using a magnifying glass and a 10× geological hand lens. E. coli colonies are royal blue/purple and coliform bacteria colonies appear to be light green. Duplicate samples were run on every tenth sample and each sample was counted twice.
Samples were taken from 24 different localities in April-May 2016, 2017 and 2018 (Figure 1). For the purpose of this study the samples will be discussed in terms of (a) overall trends (n = 24), (b) surface water (n = 8), and (c) springs (n = 16).
3.1. Physical Parameters
Physical parameters, plotted against altitude, are shown in Figure 2. The overall
Figure 2. Physical parameters of (a) pH, (b) Temperature and (c) Conductivity plotted against altitude. 2016: filled diamonds and dotted line, 2017: filled circles and dashed line, 2018: filled triangles and solid line.
trend for all samples is decreasing temperature and conductivity, and increasing pH with altitude. This same pattern is seen when the samples are separated in the surface water and springs (Figure 2). In 2016 average drinking water temperatures were 10.1 (±3.6)˚C (surface water 9.7 (±5.0)˚C and spring water 10.3 (±2.9)˚C). In all instances surface water is colder than spring water. In 2017 average drinking water temperatures were 8.5 (±3.5)˚C (surface water 7.5 (±4.0)˚C, spring water 9.1 (±3.1)˚C), while in 2018 average drinking water temperatures were 9.8 (±4.3)˚C (surface water 10.1 (±3.6)˚C, spring water 11.1 (±3.3)˚C). Water temperatures decrease with increasing altitude, with 2017 standing out with the steepest trend in temperature decrease.
In all years, pH shows a steady increase with altitude. 2016 had consistently lower pH levels than 2017 or 2018, with an average pH of all drinking water samples of 6.2 (±0.4) (vs 7.0 (±0.46) in 2017 and 6.8 (±0.4) in 2018). The average pH of surface water in 2016 was 6.3 (±0.5) (vs. 6.9 (±0.5) in 2017 and 6.8 (±0.4) in 2018) and the average for spring water was 6.2 (±0.4) (vs. 7.0 (±0.4) in 2017 and 6.7 (±0.4) in 2018).
The range in conductivity in the samples was relatively small with a maximum of 173.4 µS in 2016 and a minimum of 8.6 µS in 2017. As with pH, conductivity shows a weak positive correlation with altitude. 2016 had consistently higher conductivity levels than 2017 or 2018, with an average for all drinking water samples of 107.0 (±38.4) µS (2017: 46.1 (±23) µS; 2018: 43.3 (±19.58) µS), an average conductivity of surface water of 110.0 (±41.1) µS (2017: 58.1 (±19.1) µS; 2018: 38.2 (±26.7) µS), and the average for spring water was 105.4 (±38.5) µS (2017: 39.7 (±22.9) µS; 2018: 39.2 (±14.9) µS).
3.2. E. coli and Coliform Bacteria
All 24 drinking water samples were analyzed for Escherichia coli (E. coli) and Total Coliform bacteria, see Figure 3 and Figure 4, respectively. E. coli CFU (colony forming units per 100 ml sample) shows a weak positive correlation with altitude. The maximum number of E. coli CFU (144 CFU) were found in samples taken from a small tributary used for drinking water in the village of Toktok (~2890 m). Only one village source, Debuche (~3740 m), tested negative (0 CFU) for E. coli all three years.
Samples collected in 2016, in general, had the lowest levels of E. coli with a range between 0 and 60 CFU and an average of 7.7 (±14.3) CFU for all drinking water samples. Surface water samples (16.4 (±22.7) CFU) contain more E. coli than spring water (3.9 (±6.7) CFU). In 2017 and 2018 E. coli levels were higher. 2017 samples contained between 0 and 91 CFU and an average of 17.0 (±24.4) CFU for all samples, with surface water averaging 18.4 (±22.0) CFU and spring water averaging 16.3 (±26.0) CFU. In 2018 E. coli values ranged between 0 and 144 CFU and an average of 20.1 (±32.8) CFU for all samples, with surface water averaging 33.1 (±52.5) CFU and spring water averaging 12.6 (±15.9) CFU.
Results for CFU of Total Coliforms are similar to E. coli (Figure 4). Samples collected in 2016, in general, had the lowest levels of Total Coliforms with a range between 0 and 200 CFU and an average of 65 (±72) CFU for all drinking water samples. 2017 samples contained between 3 and 1125 CFU with an average of 322 (±341) CFU, and in 2018 Total Coliform values ranged between 0 and 377 CFU and an average of 80 (±103) CFU for all samples.
3.3. Precipitation Data for 2016, 2017 and 2018
In order to fully understand the interplay between precipitation and water contamination it is important to look at precipitation as both rain and snow. It is also important to look at both the long-term and the short-term impact of precipitation
Figure 3. E. coli plotted against location (in order of increasing altitude); (a) all samples, (b) surface water and (c) spring samples. 2016: filled diamonds and dotted line, 2017: filled circles and dashed line, 2018: filled triangles and solid line.
Figure 4. Total Coliforms plotted against location (in order of increasing altitude); (a) all samples, (b) surface water and (c) spring samples. 2016: filled diamonds and dotted line, 2017: filled circles and dashed line, 2018: filled triangles and solid line.
prior to sampling. Hence, as our sampling occurred in 2016, 2017 and 2018, precipitation data for 2015-2018 is included (Table 1). The data given here is sourced from the Government of Nepal, Ministry of Energy, Water Resources and Irrigation, Department of Hydrology and Meteorology  and comes from the meteorological station located in the village of Jiri; 1905 m asl, 27˚38'N 86˚14'E (Figure 1).
Average yearly precipitation (as total rainfall) in Jiri between 1960 and 1990 was 2353 mm . In 2015 the average precipitation was 1869 mm, in 2016 the
Table 1. Precipitation data .
Note: 2018 total annual precipitation and peak monsoon data is not included as it post-dates all sampling and is therefore not relevant.
average precipitation was 2302 mm and in 2017 the average precipitation was 1660 mm. As such, rainfall in 2016 approached normal (average) values for the region, whereas 2015 and 2017 both experienced less rainfall. The same trend is found in peak monsoon rain whereby 2015 and 2017 experienced 669 mm and 634 mm, respectively, but peak rainfall in July 2016 was over 838 mm. Similarly, snowfall in 2016 was >200 cm higher than in 2015 and 2017. In summary, yearly precipitation for 2016 was significantly higher than for 2015 and 2017 .
Short term precipitation, directly prior to sampling, showed a different trend. Looking at data for three months prior to sampling means that for 2016 and 2017 the data includes January, February and March, and for 2018 the data includes February, March and April. In 2016, the total precipitation (as rainfall) prior to sampling was 64 mm. In 2017 the total precipitation (as rainfall) prior to sampling was 73 mm, and in 2018 the three months prior to sampling experienced a total rainfall of 247 mm. The data for the month prior to sampling shows the same trend whereby 2018 experienced the most rainfall, 199 mm, whereas 2017 and 2016 were progressively dryer with 58 mm and 30 mm respectively. In respect to short term precipitation prior to sampling, 2016 was the driest of the three years sampled and 2018 was the wettest.
Looking at precipitation trends in the High Himalayas, it has been noted by several authors   that although extreme rainfall events have been increasing over the past 50 years, precipitation during the winter, pre-monsoon and post-monsoon seasons has been decreasing. GON  shows that with respect to data collected between 1960 and 1990, extreme precipitation events in the region have increased during the monsoon season, but decreased throughout the remainder of the year. This is particularly evident in 2016 whereby the pre-monsoon rains were very low, but peak monsoon rain and the overall yearly precipitation were high. In addition, overall precipitation has increased at a rate of 6.6 mm/yr since 1971, while simultaneously maximum daily temperatures have also increased by 0.52˚C per year. Current precipitation trends in the region show that more rain now falls during the monsoon season, and less precipitation (and therefore less snow) during the remainder of the year. The result is more flooding and less glacial growth  .
The quantitative assessment of drinking water in the SNPBZ is relatively new. The only previous work, by Sharma et al.  and Ghimire et al.  , focused entirely on the major rivers and lakes within the park which are not commonly used as sources for drinking water. The first studies of E. coli and coliform bacteria in drinking water conducted by Nicholson et al.   . These studies have shown that much of the drinking water, and all of the rivers, contained E. coli and coliform bacteria, especially at lower altitudes. Ghimire et al.  suggested a link between unmanaged and/or poorly managed solid waste disposal, open defecation, and water contamination, which was supported by Nicholson et al.   . This study is the first longitudinal study of drinking water quality in the SNPBZ and the results suggest a strong relationship between precipitation and drinking water contamination, which lends further support to the conclusions of Ghimire et al. .
4.1. Nepali and WHO Drinking Water Quality Standards
Nepali National Drinking Water Quality Standards allow no fecal coliforms (E. coli) in drinking water. Only one drinking water source, in the village for Debuche, met the Nepali National Drinking Water Quality standards of 0 CFU E. coli in all three years. WHO  provides a scale whereby 0 CFU E. coli is deemed compliant, 1 - 10 CFU is considered low risk, 10 - 100 CFU is considered moderate risk, and above 100 CFU is considered high risk. The majority of samples, across all years, falls into the low-moderate risk categories . 2016 samples show the least amount of fecal contamination with 7 samples containing 0 CFU and 8 samples containing fewer than 10 CFU. In fact, in 2016 all but 2 spring samples fell into the WHO low risk or compliant categories. Both 2017 and 2018 samples contained more fecal contamination, with only 2 samples in 2017 and 1 sample in 2018 containing 0 CFU of E. coli. However, again the majority of the samples are low to moderate risk. Only one sample, Toktok, contained more than 100 colonies and was classified as high risk .
There is a weak negative correlation between E. coli and altitude in both surface and spring water samples. This is interpreted to be related to both decreasing temperature with altitude and decreasing population with altitude. Lower altitude regions are warmer, have a higher population and significantly more tourists. Hence at higher altitudes, water samples are less affected by fecal contamination. These higher-altitude samples are more likely to be in low or no risk  and meet Nepali National Drinking Water Quality Standards. However, it should be noted that this does not apply to stored water as is typically found in households and teahouses.
All of the samples tested met drinking water standards (both Nepali and WHO) for physical parameters. Temperature directly correlates with altitude, hence scatter in the data is likely to be the result of surface temperature fluctuations and/or the amount of surface water in the sample tested. All of three years of spring sample temperatures decrease with altitude, with similar trend lines, and there is little difference between their average high and low temperatures. However, 2017 samples have the lowest temperatures at the highest altitudes which support the hypothesis that 2017 samples have the highest surface water contribution.
Nicholson et al.  tentatively interpreted the relationship between increasing pH with increasing altitude as being the result of the geology and water-rock interactions; however, this study presents evidence contrary to this conclusion. There are two things to consider here: firstly, all three years show an increase in pH with altitude and, secondly, 2016 consistently has the lowest pH. Given that the highest altitude rocks in the sample area are limestone, which produces higher pH water than the metamorphic units more commonly found through the field area, it is possible that contact with the limestone rock unit has resulted in the increase in pH. However, the pH of samples from 2016 is consistently lower than those from 2017 and 2018. The 2016 samples also have higher conductivity which indicates an increased water-rock reaction. If the limestone was responsible for the increasing pH then the 2016 samples should have the highest pH of all three years. Recent work by Sundriyal et al.  measured the pH of snow on the central Himalayan Dokriani Glacier. They found that the pH of pre-monsoon glacial snow ranged between 5.10 and 7.09 (with a mean of 6.25) in 2013. They returned and resampled the same locations in 2015 and found that the pre-monsoon glacial snow had a pH range between 6.32 and 8.07 with a mean of 7.01. Sundriyal et al.  do not directly discuss the implications of their pH data but they do conclude that the main source of deposition of cations on the glacier are valley dust and sea salt aerosol while the source of the major anions is mainly human activities and scavenging action of the atmosphere. The high pH of glacial snow in the Himalayan Mountains is relevant to this study as this information (combined with the fact that the surface water in the region has a higher pH than the corresponding spring water, at the same elevations) suggests that the pH of the precipitation is dominating the pH of the drinking water. This supports the interpretation that the 2016 spring samples have less surface contamination; and suggests that longer residence time in the aquifer, and long interaction with the predominately lower pH metamorphic units, has resulted in a decrease in pH.
4.2. Link between Precipitation and Bacterial Contamination
The short-term and annual precipitation data is important to the results of this study as they help elucidate community vulnerability to climate change, and emphasize the long-term risks to the >1.4 billion people living downstream of the High Himalaya Mountains.
Figure 3 clearly shows that 2016 has the lowest bacterial contamination (both E. coli and Total Coliforms) and also the lowest pH and the highest conductivity of the three years sampled. Overall Total Coliform contamination is the highest in 2017 and E. coli contamination is highest in 2018; however, for spring water 2017 has the highest contamination of both E. coli and Total Coliforms. In terms of physical parameters (Figure 2), both 2017 and 2018 are very similar with regards to pH and conductivity, and have higher pH and lower conductivity than 2016. 2018 shows a very flat elevation profiles for surface water physical parameters, particularly temperature and conductivity, whereas 2017 data shows a steep negative correlation between temperature and altitude. In contrast, data from springs in 2018 and 2017 are remarkably similar.
Based on the precipitation data (Table 1), whereby both the entire year of 2015 and the three months preceding the 2016 sampling (only 30 mm precipitation) were very dry, we suggest that the shallow springs and the surface water used for drinking water in 2016 have a higher percentage of melt water and less surface water than the two succeeding years, resulting lower levels of bacterial contamination in 2016. This also ties in with the physical parameters whereby 2016 samples have the highest conductivity and the lowest pH suggesting a longer residence time in the aquifer.
The 2017 samples clearly have the highest overall bacterial contamination. The 2016 monsoon rain was heavy, the total precipitation during 2016 was very high, and the three months preceding the sampling had a precipitation of 73 mm; this suggests that 2017 water samples include a higher percentage of precipitation/surface water than the 2016 samples.
The divergence in data from 2017 to 2018 is indicative of the different impacts of annual versus short-term precipitation. Figure 3 and Figure 4 best illustrate this difference, as the data from 2017 has the highest Total Coliform contamination in surface water?, whereas 2018 has the highest E. coli contamination in surface water, whereas both years have similar levels of E. coli in springs. Annual precipitation for 2016 approached normal levels and was significantly higher than 2017 precipitation (total precipitation of 2302 mm and 1660 mm respectively). However, the month directly preceding 2018 sampling had 199 mm of precipitation, which is almost 4 times more than in 2017 (58 mm) and 6 times more than in 2016 (30 mm). As such, the elevated levels of E. coli in the 2018 surface water reflect the higher levels of surface run-off. The levels of E. coli in the spring water data for 2018 and 2017 show a diverging patter where the contamination is similar at lower altitudes but at higher altitudes the 2018 water has less fecal contamination.
Combining the precipitation data, physical parameters, and bacterial contamination shows that there are two major factors which impact drinking water quality in the SNPBZ: annual precipitation and extreme precipitation. The higher the levels of monsoon precipitation the more fecal contamination is found the following year in the drinking water. Similarly, after a short-term extreme precipitation event fecal contamination is higher. Given that the number of extreme precipitation events during the monsoon season—and in general, overall monsoon precipitation—is rising, this poses a threat to communities during the monsoon and post-monsoon season due to both flooding  and associated water contamination. However, as the climatic trends suggest a decrease in non-monsoon precipitation  , the drinking water quality throughout the remainder of the year should be safer. Unfortunately, the decrease in non-monsoon precipitation is contributing to an overall decrease in glacial discharge  . The 2016 data suggests that during periods of little precipitation, drinking water has a higher percentage of meltwater, and a decrease in Himalayan glaciers is likely to heighten dry-season water scarcity, i.e. drought conditions and crop failure as outlined by Gain et al. . Again, this will impact not only the people of the SNPBZ and the Higher Himalayan region, but also the millions of people dependent on the Himalayan Mountains for their water resources.
4.3. Implications for the Future
Few studies have focused on seasonal precipitation patterns, catchment storage dynamics, and groundwater recharge in mountainous regions within the framework of global warming . Dettinger and Earman  state that the challenges of understanding climate-change effects on groundwater are unprecedented as neither the direct nor the indirect effects on hydrological processes and groundwater resources have been sufficiently explored. Hence the SNPBZ’s reliance on shallow groundwater (in the form of shallow springs) puts these communities in a particularly vulnerable and difficult to predict situation.
Water contamination and availability in the SNPBZ are complicated by several facets including economics, governance, environmental degradation, and climate change. Locally, there is much debate about how to develop a sustainable economy without exacerbating environmental and cultural degradation. Since 2005, the Park Management Plan   has emphasized community self-governance, including local stewardship of natural resources  and in 2018, new local governance committees and leaders within the Sherpa community are making safe drinking water a priority. Providing stakeholders with water quality data generated from studies such as this will serve to strengthen their ability to address these issues as a community and build resilience for the future.
The issue of climate change is of particular concern to the future of the region, especially the impact water on resources, and hence how water resources are managed is incredibly important. Like many mountain systems around the world, the Himalaya Mountains form the headwaters for several major river systems and provide critical water resources to people downstream  through the mechanisms of water retention and storage dominated by snowmelt runoff   . Currently, climate change has resulted in glacial melting and recession   , and resulted in changes to regional climate patterns which impact surface and ground water . The resulting changes are predicted to cause a reduction in dry-season water discharge, an increase in peak discharge , and a general decrease in water resources . Despite these threats, there are few detailed studies of the current and future impacts of global warming on Himalayan Mountain water resources.
The results of this study clearly show that the communities of the high Himalayan region, specifically the SNPBZ, are vulnerable to increased water contamination during period of high and/or extreme precipitation. These same communities are likely encounter increased water scarcity during the winter, pre- and post-monsoon seasons. It is likely that mountain communities around the globe are facing similar situations. By using quantitative water quality data to better understand how local water resources respond to climate change, government agencies will have better tools to manage these water resources, both in the high mountain regions and in downstream regions.
1) Only one drinking water source, in the village of Debuche, met the Nepali National Drinking Water Quality standards of 0 CFU E. coli across all three years. The majority of samples, across all years, fall into the low-moderate risk categories  and fail Nepali National Drinking Water Standards.
2) 2016 samples show the least amount of fecal contamination; with all but 2 spring samples in the WHO low risk or compliant categories. Both 2017 and 2018 samples contained more fecal contamination, with the majority of the samples are low to moderate risk .
3) Drinking water sourced from springs generally contains less fecal contamination than drinking water sourced from surface water.
4) The overall trend for all samples, in all years, are decreasing temperature and conductivity, and increasing pH with altitude.
5) Combining the precipitation data, physical parameters and bacterial contamination, it shows that there are two major factors which impact drinking water quality in the SNPBZ: annual precipitation and extreme precipitation. The higher the levels of monsoon precipitation are, the more fecal contamination is found the following year in the drinking water. Similarly, after a short-term extreme precipitation event, fecal contamination is higher. Fecal contamination of springs is lower after periods of low precipitation.
6) The data suggests that during a period of little precipitation, drinking water has a higher percentage of meltwater.
The long-term and downstream implications of our data suggest that drinking water resources in the region are vulnerable to climate change, particularly changes in precipitation and glacial melting. Decreasing Himalayan glaciers are likely to heighten dry-season water scarcity, while increased monsoon and extreme precipitation events increase water contamination (and flooding). This will impact not only the people of the SNPBZ and the Higher Himalayan region, but also the ~1.4 billion people dependent on the Himalayan Mountains for their water resources.
This study is located within the Sagarmatha National Park and Sagarmatha National Park Buffer Zone, Nepal (27˚56'N 86˚44'E).
The authors are grateful to the following people and organizations for their support: Micrology Labs®, Ang Norbu Sherpa (Holiday Namche Hotel), Ang Pema Sherpa (Valley View Khumjung Hotel), the Sagarmatha National Park Service and everyone that helped with sampling. This study was financially supported by Ball State University.