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 JBM  Vol.7 No.11 , November 2019
Comparative Analysis on the Haematological Parameters and Antioxidant Levels of Parboiled and Un-Parboiled Dichlorvos (DDVP)-Treated Beans
Abstract: This study comparatively evaluates the haematological and antioxidant effects of differently prepared (parboiled and un-parboiled) beans treated with sniper (i.e. a dichlorvos insecticide) and fed to wistar albino rats. Thirty (30) male wistar albino rats of known body weight were assigned into six (6) groups of 5 each. The groups received: 70 g and 30 g of un-parboiled beans (groups 1 & 2), 70 g and 30 g (groups 3 & 4) of parboiled beans compounded in the pellets as feed stock, beans only and standard feed for a period of 30 days. The rats were euthanized, and blood samples were collected after the termination of the study. Dichlorvos significantly decreased (P < 0.05) the concentration of the antioxidant enzymes (GSH, GPx, SOD, CAT) in the groups fed with un-parboiled beans unlike the groups fed with parboiled beans which were non-significantly decreased. There was significant increase in the malondialdehyde concentration of the groups fed with un-parboiled beans. Dose dependent variations were seen in the packed cell volume (PCV), white blood cell (WBC), haemoglobin (Hb) and platelet. But a reduced concentration of red blood cell count (RBC) for the un-parboiled groups and an increase in the parboiled group were seen, although both were not significant (P < 0.05).
Cite this paper: Chinedu, N. and Darlington, N. (2019) Comparative Analysis on the Haematological Parameters and Antioxidant Levels of Parboiled and Un-Parboiled Dichlorvos (DDVP)-Treated Beans. Journal of Biosciences and Medicines, 7, 65-75. doi: 10.4236/jbm.2019.711006.
References

[1]   Damalas, C.A. and Eleftherohorinos, I.G. (2011) Pesticide Exposure, Safety Issues, and Risk Assessment Indicators. International Journal of Environmental Research and Public Health, 8, 1402-1419.
https://doi.org/10.3390/ijerph8051402

[2]   Banjo, A. D., Aina, S. A., and Rije, O. I. (2010) Farmers’ Knowledge and Perception towards Herbicides and Pesticides Usage in Fadama Area of Okun-Owa, Ogun State of Nigeria. African Journal of Basic and Applied Science, 2, 188-194.

[3]   Olabode, O.S., Adeshina, G.O. and Olapeju, T.R. (2011) A Survey of Agricultural Chemicals Available to Farmers in South Western Nigeria. International Journal of Agricultural Economic and Rural Development, 4, 12.

[4]   Delcour, I., Spanoghe, P. and Uyttendaele, M. (2015) Literature Review: Impact of Climate Change on Pesticide Use. Food Research International, 68, 7-15.
https://doi.org/10.1016/j.foodres.2014.09.030

[5]   Aprea, C., Strambi, M., Novelli, M.T., Lunghini, L. and Bozz, N. (2000) Biologic Monitoring of Exposure to Organophosphorus Pesticides in 195 Italian Children. Environmental Health Perspectives, 108, 521-525.
https://doi.org/10.1289/ehp.00108521

[6]   Oyenuga, V.A. (1968) Nigeria’s Foods and Feeding Stuffs, Their Chemistry and Nutritive Value. 3rd Edition, Ibadan University Press, Ibadan, 99.

[7]   Hung, Y.C., Mc Watters, K.H., Phillips, R.D. and Chinnan, M.S. (1990) Effects of Predecortications Drying Treatment on the Microstructure of Cowpea Products. Journal of Food Science, 55, 774-807.
https://doi.org/10.1111/j.1365-2621.1990.tb05227.x

[8]   Das, S. (2013) A Review of Dichlorvos Toxicity in Fish. Current World Environment, 8, 143-149.
https://doi.org/10.12944/CWE.8.1.08

[9]   Musa, U., Hati, S.S., Mustapha, A. and Magaji, G. (2010) Dichlorvos Concentrations in Locally Formulated Pesticide (Otapiapia) Utilized in Northeastern Nigeria. Scientific Research and Essay, 5, 49-54.

[10]   Koutros, S., Mahajan, R., Zheng, T., Hoppin, J.A., Ma, X., Lynch, C.F., Blair, A. and Alavanja, M.C. (2008) Dichlorvos Exposure and Human Cancer Risk: Results from the Agricultural Health Study. Cancer Causes & Control, 19, 59-65.
https://doi.org/10.1007/s10552-007-9070-0

[11]   Hazarika, A., Sarkar, S.N., Hajare, S., Kataria, M. and Malik, J.K. (2003) Influence of Malathion Pretreatment on the Toxicity of Anilofos in Male Rats: A Biochemical Interaction Study. Toxicology, 185, 1-8.
https://doi.org/10.1016/S0300-483X(02)00574-7

[12]   Mitchell, D.C., Lawrence, F.R., Hartman, T.J. and Curran, J.M. (2009) Consumption of Dry Beans, Peas, and Lentils Could Improve Diet Quality in the US Population. Journal of the American Dietetic Association, 109, 909-913.
https://doi.org/10.1016/j.jada.2009.02.029

[13]   Sharma, P. and Singh, R. (2012) Dichlorvos and Lindane Induced Oxidative Stress in Rat Brain: Protective Effects of Ginger. Pharmacognosy Research, 4, 27-32.
https://doi.org/10.4103/0974-8490.91031

[14]   Verma, R.S., Mehta, A. and Srivastava, N. (2009) Comparative Studies on Chlorpyrifos and Methyl Parathion Induced Oxidative Stress in Different Parts of Rat Brain: Attenuation by Antioxidant Vitamins. Pesticide Biochemistry and Physiology, 95, 152-158.
https://doi.org/10.1016/j.pestbp.2009.08.004

[15]   Gultekin, F., Delibas, N., Yasar, S. and Kilinc, I. (2001) In Vivo Changes in Antioxidant Systems and Protective Role of Melatonin and a Combination of Vitamin C and Vitamin E on Oxidative Damage in Erythrocytes Induced by Chlorpyrifos-Ethyl in Rats. Archives of Toxicology, 75, 88-96.
https://doi.org/10.1007/s002040100219

[16]   Obida, M.G., Hati, S.S., Dimari, G.A. and Ogugbuaja, V.O. (2012) Pesticide Residues in Bean Samples from Northeastern Nigeria. ARPN Journal of Science and Technology, 2, 79-84.

[17]   Wallin, B., Rosengren, B., Shertzer, H.G. and Camejo, G. (1993) Lipoprotein Oxidation and Measurement of Thiobarbituric Acid Reacting Substances Formation in a Single Microtiter Plate: Its Use for Evaluation of Antioxidants. Analytical Biochemistry, 208, 10-15.
https://doi.org/10.1006/abio.1993.1002

[18]   Arthur, J.R. and Boyne, R. (1985) Superoxide Dismutase and Glutathione Peroxidase Activities in Neutrophils from Selenium Deficient and Copper Deficient Cattle. Life Sciences, 36, 1569-1575.
https://doi.org/10.1016/0024-3205(85)90381-9

[19]   Sinha, A.K. (1972) Colorimetric Assay of Catalase. Analytical Biochemistry, 47, 389-394.
https://doi.org/10.1016/0003-2697(72)90132-7

[20]   Paglia, D.E. and Valentine, W.N. (1967) Studies on the Quantitative and Qualitative Characterization of Erythrocyte Glutathione Peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158-169.

[21]   Exner, R., Wessner, B., Manhart, N. and Roth, E. (2000) Therapeutic Potential of Glutathione. Wiener klinische Wochenschrift, 112, 610-616.

[22]   Ochei, J. and Kolhatkar, A. (2008) Medical Laboratory Science, Theory and Practices. Tata McGraw-Hill, 311-347.

[23]   Wani, W.Y., Gudup, S., Sunkaria, A., Bal, A., Singh, P.P., Kandimalla, R.J., Sharma, D.R. and Gill, K.D. (2011) Protective Efficacy of Mitochondrial Targeted Antioxidant Mitochondria against Dichlorvos Induced Oxidative Stress and Cell Death in Rat Brain. Neuropharmacology, 61, 1193-1201.
https://doi.org/10.1016/j.neuropharm.2011.07.008

[24]   Kanu, K.C., Ijioma, S. and Atiata, O. (2016) Haematological, Biochemical and Antioxidant Changes in Wistar Rats Exposed to Dichlorvos Based Insecticide Formulation Used in Southeast Nigeria. Toxics, 4, 28.
https://doi.org/10.3390/toxics4040028

[25]   Oral, B., Guney, M., Demirin, H., Ozguner, M., Giray, S.G., Take, G., Mungan, T. and Altuntas, I. (2006) Endometrial Damage and Apoptosis in Rats Induced by Dichlorvos and Ameliorating Effect of Antioxidant Vitamins E and C. Reproductive Toxicology, 22, 783-790.
https://doi.org/10.1016/j.reprotox.2006.08.003

[26]   Prakasam, A., Sethupathy, S. and Lalitha, S. (2001) Plasma and RBCs Antioxidant Status in Occupational Male Pesticide Sprayers. Clinica Chimica Acta, 310, 107-112.
https://doi.org/10.1016/S0009-8981(01)00487-9

[27]   Hsu, D.Z., Hsu, C.H., Huang, B.M. and Liu, M.Y. (2001) Abamectin Effects on Aspartate Aminotransferase and Nitric Oxide in Rats. Toxicology, 165, 189-193.
https://doi.org/10.1016/S0300-483X(01)00434-6

[28]   Poljsak, B. and Jamik, P. (2010) Methodology for Oxidative State Detection in Biological Systems. In: Kozyrev, D. and Slutsky, V., Eds., Handbook of Free Radicals: Formation, Types and Effects, Cell Biology Research Progress Series, Nova Science, New York.

[29]   de Lamirande, E., Jiang, H., Zini, A., Kodama, H. and Gagnon, C. (1997) Reactive Oxygen Species and Sperm Physiology. Reviews of Reproduction, 2, 48-54.

[30]   Dwivedi, N. and Flora, S.J. (2015) Sub-Chronic Exposure to Arsenic and Dichlorvos on Erythrocyte Antioxidant Defense Systems and Lipid Peroxidation in Rats. Journal of Environmental Biology, 36, 383-391.

[31]   Edem, V.F., Kosoko, A., Akinyoola, S.B., Owoeye, O., Rahamon, S.K. and Arinola, O.G. (2012) Plasma Antioxidant Enzymes, Lipid Peroxidation and Hydrogen Peroxide in Wistar Rats Exposed to Dichlorvos Insecticide. Archives of Applied Science Research, 4, 1778-1781.

[32]   Ojo, A.O., Oyinloye, B.E., Ajiboye, B.O., Ojo, A.B., Akintayo, C.O. and Okezie, B. (2014) Dichlorvos Induced Nephrotoxicity in Rat Kidney: Protective Effects of Alstonia Boonei Stem Bark Extract. International Journal of Pharmacognosy, 1, 429-437.

[33]   Tiryaki, O. and Temur, C. (2010) The Fate of Pesticide in the Environment. Journal of Biodiversity and Environmental Sciences, 4, 29-38.

[34]   Akomas, S.C., Okafor, A.I. and Ijioma, S.N. (2014) Glucose Level, Haematological Parameters and Lipid Profile in Ficus. sur Treated Diabetic Rats. Comprehensive Journal of Agricultural and Biological Science, 2, 5-11.

[35]   Alia, M., Horcajo, C., Bravo, L. and Goya, L. (2003) Effect of Grape Antioxidant Dietary Fiber on the Total Antioxidant Capacity and the Activity of Liver Antioxidant Enzymes in Rats. Nutr. Res, 23, 1251-1267.
https://doi.org/10.1016/S0271-5317(03)00131-3

 
 
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