Mauritia arabica is mainly distributed on the coast of southeastern China, in rocky or coral reef substrate around the low tide zone . It is harvested either in summer or autumn, and applied as the raw Chinese tradition medicine (TCM) after the flesh is removed, the left shells are washed, dried and mashed. The preparation of MAS as medicine can refer to the appendix IID in the Pharmacopoeia of the People’s Republic of China. Briefly, the small pieces of dried and mashed MAS are calcined first above 600˚C in furnaces or reactors and then cooled down to room temperature. The resulted fragile MAS are granulated into powder before extraction to make into the “medicinal cocktail”.
Research shows that the major components of MAS are calcium carbonate (95.82% - 97.24%), protein (about 0.5%) and trace elements (about 2%) etc. The main elementary of MAS extraction are Al, Fe, Na, Mn, K, Mg and Ba. Aspartic acid, Glutamic acid, Glycine and leucine are the dominating ones among the whole sixteen types of amino acids from protein . An ancient TCM book Qian Xin Yao Fang recorded that the extractions from calcined MAS have different medical functions compared to the un-calcined extractions. Therefore, it is greatly important to explore the MAS calcination processes for pharmaceutical usages and their influences on their biological functions. On the other hand, it is known that feeding hypertensive rats with the extracted water extraction from MAS led to the decreased blood pressure . However, the chemistry and physics characteristic changes between un-calcined and calcined MAS remain unclear. In this investigation, the calcined pyrolysis of MAS utilizing thermal analyses including thermogravimetry (TG) and derivative thermogravimetry (DTG) was studied. The relevant kinetic characteristics were also identified to provide potential guidelines for the optimal pyrolysis temperature to convert MAS into functional biomedicine materials.
2. Material and Methods
2.1. MAS Decomposition and TG/DTG Analysis
MAS were purchased from Tong Ren Tang Pty Ltd (China) and granulated into fine powders (particle size less than 0.149 mm, 100 mesh). The experimental apparatus performing TG/DTG analysis was a NETZSCH TG/DTA model STA-409C analyzer (Netzsch Inc., Selb, Germany). About 10 mg of MAS samples were performed in the furnace of the thermobalance under the programming temperature from room temperature to 900˚C at heating rates of 5˚C/min, 10˚C/min, 20˚C/min in air. The experiment sweeping gas was high purity N2 at a flowing rate of 70 mL/min in ambience pressure.
2.2. Kinetic Analysis of the MAS Decomposition Process
The decomposition reaction rate of MAS can be described by KAS equation   shown by Equation (1), as the main composition in MAS is calcium carbonate. And the decomposition reaction rate also can be described by Ozawa-Flynn-Wall (OFW) equation   shown by Equation (2):
where, β is the heating rate, T is the absolute temperature in K, Ea is the activation energy of the reaction, α is the degree of conversion value, A is Arrhenius parameter, R is the universal constant.
For KAS equation, each α, ln(βi/T2) is plotted vs. 1/T, gives a straight line with slope Ea/R, and thus the values Ea are obtained as a function of the conversion. For OFW equation each α, ln(βi) is plotted vs. 1/T, also gives a straight line with slope −1.0516Ea/R, and thus the values Ea are obtained.
2.3. Physicochemical Characterizations
According to the Chinese Pharmacopoeia, 600˚C is the lowest calcined temperature. To obtained the different calcined MAS, MAS was heated in air at the rate of 10˚C/min from room temperature until 600˚C and 800˚C , held for 1 h before cooling with pure nitrogen, respectively. The resulted calcined MAS samples, named MAS600 and MAS800, their physicochemical properties were further analyzed.
For FTIR spectroscopic characterization, approximately 1 mg of MAS or calcined MAS sample was grinded with approximately 100 mg of spectroscopic-grade KBr, and then was pressed into 1 mm pellets. The analysis was conducted by a FTIR spectrometer equipped with a DTGS detector (MB104, ABB Bomen Co., Quebec, Canada). Each spectrum was recorded at the resolution of 4 cm−1 with 64 co-added scans in a frequency range of 500 to 4000 cm−1. The FTIR data was collected by Spectrum v5.0 software from Perkin Elemer company (USA). OMNIC 6.0 software (Thermo Electron Corporation, Madison, WI, USA) was applied to report the data, including baseline fitting with automatic smooth and min-max normalization of 4000 cm−1.
Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) obvervation were carried out using a Scanning Electron Microscope JEOL JSM-76360LV instrument equipped with X-ray powder diffraction (XRD) tests. For SEM analysis, the operating voltage was from 10 to 20 kV. XRD patterns were collected on a diffractometer (D/max 2500, Japan, Rigaku) with Cu Kα radiation (λ = 1.5406 Å）at room temperature. The operating voltage and current were 36 kV and 30 mA, respectively, and the scan speed was 2˚/min in the two-theta range of 15˚ - 60˚.
3. Results and Discussion
3.1. MAS Decomposition Process
MAS was calcined from room temperature to 900˚C at different heating rates (β) of 5˚C/min, 10˚C/min, and 20˚C/min, respectively. TG and DTG analyses were displayed in Figure 1 and the decomposition characteristic parameters of specific MAS pyrolysis were listed in Table 1.
Figure 1. TG/ DTG curves for MAS samples at different heating rate. (a) TG curves; (b) DTG curves (inlet figure shows the scaled second stage of mass weight loss).
Table 1. Specific characteristic parameters of MAS thermal decomposition.
*Ti, the initial decomposition temperature; Tp, the temperature of maximum weight loss rate; Te, the end temperature of the stage; Mass%, the weight loss at the stage; Mt%, the total loss.
In the temperature-programmed pyrolysis process, MAS was gradually calcined with protein thermal decomposition and phase transition. As shown in Figure 1(a), the major mass weight loss commences at about 600˚C and ends at around 800˚C with different heating rates. Moreover, MAS pyrolysis has three stages (Figure 1(b)). The first stage starts from the room temperature till 142˚C with the mass weight loss of 0.04% - 0.26%; the second stage of weight loss of 1.14% - 2.37% ranging from 270˚C to 553˚C and the third weight loss of 43.27% - 44.73% between 600˚C and 800˚C. The first stage of the weight loss signified the water evaporation in the shell. Besides, the second stage is corresponding to the decomposition of organics and the endothermic phase transformation from aragonite crystal to calcite crystal. It is reported that the natural aragonite phase of CaCO3 transition temperature ranges from 450˚C to 500˚C . Moreover, the phase transition of aragonite to calcite in cockle shell, coral and other marine shells, occurs at a lower temperature than that of the phase transition in aragonite of mineral origin   . The third mass loss stage starts at around 600˚C and ends at 800˚C, which varied according to the heating rate. As shown in Figure 1(b), at the heating rate of 5˚C/min, the peak temperature is 699˚C with the maximum weight loss rate of −0.358 mg/min. When the heating rate is 10˚C/min and 20˚C /min, the peak temperature is 724˚C with maximum weight loss rate of –0.555 mg/min, and the peak temperature is 773˚C with maximum weight loss rate of −1.464 mg/min, respectively. The results indicate the total decomposition of calcium carbonate in MAS to CaO and signify the release of CO2 from calcium carbonate. Meanwhile, it was assumed that the rapid heating rate could accelerate the decomposition of the organic compounds in the shell, which in turn required higher decomposition temperatures.
3.2. Kinetic Analysis of MAS Decomposition
Further dynamics analysis was conducted by applying the KAS model to calculate the activation energy at different decomposition stages. Based on the Equations (1) and (2), the linear plots of ln(βi/T2) or ln(βi) vs. the inverse temperature and the activation energy Ea values obtained as a function of the conversion are shown in Figure 2 and Figure 3. The calculated activation energy of the second and third stages of decomposition using KAS and OFW method is shown in Figure 4.
As shown in Figure 4(a), at the second stage of protein decomposition and phase transition, the average Ea value is 58.13 kJ/mol (average R = 0.947, KAS method) and 64.34 kJ/mol (average R = 0.963, OFW method), respectively. Comparing with the literature reports, the average Ea value is higher than the biomaterial with more plant proteins, whose Ea value calculated by Coasts-Redfern (CR) method is 81 kJ/mol in 200˚C - 460˚C . The third stage of calcination corresponds to the decomposition of CaCO3. The average Ea value of MAS decomposition is 181.27 kJ/mol (average R = 0.969, KAS method) and 188.47 kJ/mol (average R = 0.972, OFW method), respectively. Comparing with the literature reports, the average Ea value of pure calcium carbonate decomposition calculated by OFW method is 203 kJ/mol and 194.1 kJ/mol (Friedman method) . It shows that the average activation energy of MAS decomposition is generally lower than that of the pure calcium carbonate decomposition, because MAS contains not only calcium carbonate, but also organic macromolecules, such as amino acid and trace elements, which may play important roles in medication. Therefore, the elements retained in MAS before and after calcination are further measured by FTIR and XDR in the following.
Figure 2. Fitting curves to kinetic model proposed by KAS (a) and OFW (b) method at the second stage of decomposition.
Figure 3. Fitting curves to kinetic model proposed by KAS (a) and OFW (b) method at the third stage of decomposition.
Figure 4. Ea valune-activation energy of MAS pyrolysis proposed by KAS and OFW method. (a) Second stage decomposition; (b) third stage decomposition.
3.3. FTIR Analyses
To fully understand the function ingredients of MAS as a medicine, the compositions of MAS before and after calcination are further identified by FTIR tests. In Figure 5, the comparison of FTIR spectra between untreated and calcined MAS in the range of 4000 - 500 cm−1 was illustrated.
The major components of MAS are calcium carbonate. The carbonate ions in the MAS are demonstrated by the internal vibration modes of the ions, 710 cm−1 (γ4 ), 860 cm−1 (γ2 ), 1082 cm−1 (γ1 ), 1477 cm−1 (γ3 ), 1789 cm−1 (γ3 ), and 2521 cm−1 (γ1 + γ3) . The characteristic absorption peaks of aragonite CaCO3 are at 862 cm−1 (γ2 ) and 712 cm−1 (γ4 ) . MAS FTIR band at 860 cm−1 (γ2 ) and 710 cm−1 (γ4 ) are attributed to the strong complexation of aragonite crystal in MAS with organic species. Compared to the untreated MAS, the absorption band of ions at 860 cm−1 (γ2 ), is shifted to 874 cm−1 in MAS600 and MAS800, and the band at 1477 cm−1 (γ3 ) is shifted to 1437 cm−1 for MAS600 and 1429 cm−1 for MAS800, respectively. The internal vibration modes of the untreated MAS ions at 1082 cm−1 (γ1 ) are not detected in MAS600 and MAS800 and 2521 cm−1 (γ1 + γ3) bands are shifted to 2514 cm−1 (γ1 ) in MAS600 and MAS800. FTIR spectra of calcite CaCO3 shows that the absorption bands are at the wavenumber 710 cm−1 (γ4 ), 875 cm−1 (γ2 ), 1427 cm−1 (γ3 ), 1800 cm−1 (γ3 ) and 2513 cm−1 (γ1 + γ3). Considering that the phase transition of aragonite temperature ranges from 270˚C to 360˚C, the CaCO3 in MAS600 and MAS800 is in the phase of calcite. Furthermore, the peak at 3639 cm−1 is assigned to the OH group and narrowed significantly, due to the water evaporation, but some remains in the porous calcinated shell . Besides, the calcination to 800˚C leads to further break down of organic species,
3.4. SEM-EDS Analysis
The morphology and structure of untreated and calcined MAS were further observed by scanning electron microscopy (SEM) and shown in Figure 6. It demonstrates the particle morphology of un-treaded MAS (Figure 6(a)). MAS is constructed as ‘‘brick-mortar’’ microstructure in which the brick is the organic-minerals containing inorganic components, such as amino acids as the mortar. The particle patterns of MAS600 and MAS800 in Figure 6(b) and Figure 6(c), indicate the decomposition products have similar particles morphology. The most obvious difference is that the “mortar” microstructure is disappeared and covered by a layer of small particles. Meanwhile, the number of the small particles is increased in MAS800.
By EDS measurements, the difference in major elements between un-calcined and calcined samples was identified (Figure 7). It can be seen that in uncalcined shell, the elements such as Ca, K, P and Na were detected in the shell, but their concentrations decrease after heating, except Ca. Moreover, the signal of element Ca, which has a significant enhancement after calcination, can be attributed to the decomposition of CaCO3 .
3.5. XRD Analysis
The X-ray powder diffraction (XRD) patterns of un-calcined and calcined MAS were collected (Figure 8), where the diffraction pattern of un-clacined MAS with the peaks of (111), (021) and (012). They match with the JCPDS PDF file No.
Figure 5. FTIR spectra of MAS samples calcined at different temperature. MAS: un-calcined Mauritia arabica shell; MAS600: Mauritia arabica shell calcined at 600˚C; MAS800: Mauritia arabica shell calcined at 800˚C.
Figure 6. SEM images of un-calcined and calcined MAS. (a) MAS: un-calcined Mauritia arabica shell; (b) MAS600: Mauritia arabica shell calcined at 600˚C; (c) MAS800: Mauritia arabica shell calcined at 800˚C.
Figure 7. Major elements contents of samples from EDS spectrum.
Figure 8. The XRD patterns of different samples.
41-1475 (aragonite -CaCO3) indicating that the phase of CaCO3 in un-calcined MAS is only aragonite. The strongest diffraction peak of aragonite in MAS is (111) crystal peak. Figure 8(b) shows the MAS600 diffraction pattern, which matches the JCPDS PDF file No. 05-0586 (calcite-CaCO3). The strong peak of (104), five medium peaks (110), (113), (202), (018), (116) and two weak peaks (012), (006) in MAS600 indicate that aragonite is disappeared and more stable phase calcite CaCO3 in MAS600 appeared. Figure 8(c) shows the diffraction pattern of MAS800. This pattern matches two JCPDS PDF files No. 05-0586 (calcite-CaCO3) and No. 04-0733 (portlandite-Ca(OH)2). The peaks of (001), (101), (002) and (111) indicate that Ca(OH)2 appeared, which resulted from two reactions CaCO3 → CaO + CO2 and CaO + H2O → Ca(OH)2     . XRD results are well consistent with FTIR results shown in Figure 5.
Mauritia arabica shell (MAS) has a laminated structure and the major components are calcium carbonate and protein. Calcination process is an important operation for taking natural Mauritia arabica shell as raw Chinese traditional medicine in pharmaceutical engineering. The pyrolysis corresponds mainly to the decomposition of protein, the phase transition of calcium carbonate from aragonite to calcite, and the decomposition of calcium carbonate. The activation energy in the heating duration is 58.13 kJ/mol for crystalline transformation and 181.27 kJ/mol for decomposition respectively, by applying the KAS model. At around 553˚C, the endothermic phase transformation from aragonite crystal to calcite crystal is completely finished. At 600˚C, the calcium carbonate in MAS begins to decompose. In conclusion, the present study provides valuable information for the determination of calcination temperatures in the pharmaceutical treatments of MAS.
This work is financially supported by the China Scholarship Fund (20135045).
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