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 JBM  Vol.7 No.11 , November 2019
Mutagenic Potential of Medicinal Polyherbal Preparations
Abstract: Extracts of twelve medicinal polyherbal preparations in powder form sold at retail shops were tested for mutagenicity using Ames in vitro test. Five of the polyherbal preparations were found to be mutagenic (p ≤ 0.05) at 250 μg/ml. Two mutagenic preparations comprised of traditionally used medicinal plants with no mutagenicity property being reported. However, one polyherbal preparation which contained one mutagenic plant was found not mutagenic. Under the conditions of this study, it can be concluded that some polyherbal preparations were potentially mutagenic and mutagenicity of polyherbal preparation cannot always be deduced from the mutagenicity status of each individual plant components of the polyherbal preparations.
Cite this paper: Razak, M. (2019) Mutagenic Potential of Medicinal Polyherbal Preparations. Journal of Biosciences and Medicines, 7, 10-19. doi: 10.4236/jbm.2019.711002.
References

[1]   Van der Hoven, J.C. (1985) Occurrence and Detection of Natural Mutagens and Modifying Factors in Food Products. Princess Takamatsu Symposium, 16, 119-137.

[2]   Guil, J.L., Rodriguez-Garcia, I. and Torija, E. (1997) Nutritional and Toxic Factors in Selected Wild Edible Plants. Plant Foods for Human Nutrition, 51, 99-107.
https://doi.org/10.1023/A:1007988815888

[3]   Yen, G.C., Chen, H.F. and Peng, H.H. (2001) Evaluation of the Cytotoxicity, Muta-genicity and Antimutagenicity of Emerging Edible Plants. Food and Chemical Toxicology, 39, 1045-1053.
https://doi.org/10.1016/S0278-6915(01)00053-9

[4]   Morimoto, I., Watanabe, F., Osawa, T., Okitsu, T. and Kada, T. (1982) Mutagenicity Screening of Crude Drugs with Bacillus subtilisrecassay and Salmonella/Microsome Reversion Assay. Mutation Research, 97, 81-102.
https://doi.org/10.1016/0165-1161(82)90007-3

[5]   Riazuddin, S., Malik, M.M. and Nasim, A. (1987) Mutagenicity Testing of Some Medicinal Herbs. Environmental and Molecular Mutagenesis, 10, 141-148.
https://doi.org/10.1002/em.2850100204

[6]   Yin, X.-J., Liu, D.-X., Hecknan, W. and Yu, Z. (1991) A Study on the Mutagenicity of 102 Raw Pharmaceuticals Used in Chinese Traditional Medicine. Mutation Research, 260, 73-82.
https://doi.org/10.1016/0165-1218(91)90082-W

[7]   Schimmer, O., Kruger, A., Paulini, H. and Haefele, F. (1994) An Evaluation of 55 Commercial Plant Extracts in the Ames Mutagenicity Test. Pharmazie, 49, 448-451.

[8]   Ames, B.N. (1983) Dietary Carcinogenesis and Anticarcinogens. Science, 221, 1256-1264.
https://doi.org/10.1126/science.6351251

[9]   Ames, B.N., McCann, J. and Yamasaki, E. (1975) Methods for Detecting Carcinogens and Mutagens with Salmonella/Mammalian-Microsome Mutagenicity Test. Mutation Research, 31, 347-364.
https://doi.org/10.1016/0165-1161(75)90046-1

[10]   McCann, J., Choi, E., Yamasaki, E. and Ames, B.N. (1975) Detection of Carcinogens in the Salmonella/Microsome Test. Assay of 300 Chemicals. Proceedings of the National Academy of Sciences of the United States of America, 72, 5135-5139.
https://doi.org/10.1073/pnas.72.12.5135

[11]   Zeiger, E., Hasemann, J.K., Shelby, M.D., Margolin, B.H. and Tennant, R.W. (1990) Evaluation of Four in Vitro Genetic Toxicity Tests for Predicting Rodent Carcinogenicity: Confirmation of Earlier Results with 41 Additional Chemicals. Environmental Molecular Mutagenesis, 16, 1-14.
https://doi.org/10.1002/em.2850160502

[12]   Mohd Fuat, A.R., Aidoo, K.E., Calvert, T.W. and Candlish, A.A.G. (2006) Mycoflora, Cytotoxicity and DNA Interaction of Polyherbal Products from Malaysia. Pharmaceutical Biology, 44, 23-31.
https://doi.org/10.1080/13880200500530500

[13]   Valledo, L., Monica, E., Monica, M., Ella, N., Maria, J.C. and Wolfram, W. (2014) A Universal Protocol for the Combined Isolation of Metabolites, DNA, Long RNAs, Small RNAs, and Proteins from Plants and Microorganisms. The Plant Journal, 79, 173-180.
https://doi.org/10.1111/tpj.12546

[14]   Prozesky, E.A., Meyer, J.J.M. and Louw, A.I. (2001) In Vitro Antiplasmodial Activity and Cytotoxicity of Ethnobotanically Selected South African Plants. Journal of Ethnopharmacology, 76, 239-245.
https://doi.org/10.1016/S0378-8741(01)00245-8

[15]   Zeiger, E. and Mortelmans, K. (1999) The Salmonella (Ames) Test for Mutagenicity. Current Protocols in Toxicology, 3, 1-29.
https://doi.org/10.1002/0471140856.tx0301s00

[16]   Gilbert, R.I. (1980) The Analysis of Fluctuation Tests. Mutation Research, 74, 283- 289.
https://doi.org/10.1016/0165-1161(80)90168-5

[17]   De Carvalho, M.C.R.D., Barca, F.N.T.V., Lima, L.F.A. and De Medeiros, S.R.B. (2003) Evaluation of Mutagenic Activity in an Extract of Pepper Tree Stem Bark (Schinus terebinthifolius Raddi). Environmental and Molecular Mutagenesis, 42, 185-191.
https://doi.org/10.1002/em.10183

[18]   Mohd-Fuat, A.R., Aidoo, K.E. and Candlish, A.G.G. (2007) Mutagenic and Cytotoxic Properties of Three Herbal Plants from Southeast Asia. Tropical Biomedicine, 24, 49-59.

[19]   Cui, R., He, J., Wang, B., Zhang, F., Chen, G., Yin, S. and Shen, H. (2003) Suppressive Effects of Astragalus membranaceus Bunge on Chemical Hepatocarcinogenesis in Rats. Cancer Chemotherapy and Pharmacology, 51, 75-80.
https://doi.org/10.1007/s00280-002-0532-5

[20]   Caesar, L.K. and Cech, N.B. (2019) Synergy and Antagonism in Natural Product Extracts: When 1 + 1 Does Not Equal 2. Natural Product Reports, 36, 869-888.
https://doi.org/10.1039/C9NP00011A

[21]   Sarkar, D., Talukder, G. and Sharma, A. (1996) Plant Extracts as Modulators of Genotoxic Effects. The Botanical Review, 62, 275-300.
https://doi.org/10.1007/BF02856614

 
 
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