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 JBM  Vol.7 No.11 , November 2019
Long-Term Nikotine Administration Impairs Blood Parameters and Femoral Bone Tissue Structure in an Osteoporosis Model of Rats
Abstract: Osteoporosis is a systemic bone disease that results in the loss of bone mass and impared bone structure. Animal models for osteoporosis are generated by ovariectomy. Adult female Sprague-Dawley rats were divided into control, bilateral ovariectomy and bilateral ovariectomy and subcutaneous nicotine administered (received nicotine sulphate, 2 mg/kg) groups, daily for 28 days. At the end of the period, rats were sacrified under anesthesia blood samples were taken and femoral tissues were dissected. Estrogen, calcium and alkaline phosphatase levels were measured. Tissue samples were prepared for histopathogical examination. Sections were stained with Hematoxylin and Eosin and examined under light microcope. Biochemical parameters were decreased depending on the overiectomy, also decrement was notable with nicotine intake. In the ovariectomy group; increased inflammatory cells with degenerative changes around the femoral compact bone and dilatation of osteon structures in bone trabeculae and apoptotic changes in osteocyte cells in bone lacuna were apparent. In the ovariectomy with nicotine administration group, excessive dilatation of the havers lamellae in the compact bone region, increased osteoclastic activity, picnosis and apoptotic nucleus of the osteoclastic cells located in the lacuna, and increased collagen fibers in the matrix were observed. We suggest that ovariectomy and nicotine administration together effect estrogen and calcium metabolism negatively, stimulate alterations in the structural properties of bone matrix, also affect osteocyte development and bone lamellar structure that may accelerate osteoporosis development.
Cite this paper: Baloglu, M. and Özkorkmaz, E. (2019) Long-Term Nikotine Administration Impairs Blood Parameters and Femoral Bone Tissue Structure in an Osteoporosis Model of Rats. Journal of Biosciences and Medicines, 7, 1-9. doi: 10.4236/jbm.2019.711001.
References

[1]   Ishimi, Y. (2015) Osteoporosis and Lifestyle. Journal of Nutritional Science and Vitaminology, 61, S139-S141.
https://doi.org/10.3177/jnsv.61.S139

[2]   Ochi, H. and Takeda, S. (2011) Animal Models for Bone and Joint Disease. The Genetically-Modified Mice as a Tool for Osteoporosis Research. Clinical Calcium, 21, 226-232.

[3]   Oheim, R., Schinke, T., Amling, M. and Pogoda, P. (2016) Can We Induce Osteoporosis in Animals Comparable to the Human Situation? Injury, 47, S3-S9.
https://doi.org/10.1016/S0020-1383(16)30002-X

[4]   Quinn, R. (2005) Comparing Rat’s to Human’s Age: How Old Is My Rat in People Years? Nutrition, 21, 775-777.
https://doi.org/10.1016/j.nut.2005.04.002

[5]   Komori, T. (2015) Animal Models for Osteoporosis. European Journal of Pharmacology, 759, 287-294.
https://doi.org/10.1016/j.ejphar.2015.03.028

[6]   Christiansen, C. and Lindsay, R. (1990) Estrogens, Bone Loss and Preservation. Osteoporosis International, 1, 7-13.
https://doi.org/10.1007/BF01880410

[7]   Pacifici, R. (2008) Estrogen Deficiency, T Cells and Bone Loss. Cellular Immunology, 252, 68-80.
https://doi.org/10.1016/j.cellimm.2007.06.008

[8]   Saldanha, J.B., Pimentel, S.P., Casati, M.Z., Sallum, A.W., Sallum, E.A. and Nociti, F.H. (2004) Histologic Evaluation of the Effect of Nicotine Administration on Bone Regeneration. A Study in Dogs. Brazilian Oral Research, 18, 345-349.
https://doi.org/10.1590/S1806-83242004000400013

[9]   Baron, J.A. (1996) Beneficial Effects of Nicotine and Cigarette Smoking: The Real, the Possible and the Spurious. British Medical Bulletin, 52, 58-73.
https://doi.org/10.1093/oxfordjournals.bmb.a011533

[10]   Yorgancilar, E., Tunik, S., Deveci, E., Gun, R., Bakir, S., Kinis, V., et al. (2012) The Effects of Systemic Use of Nicotine on the Rat Nasal Mucosa: A Histopathologic and Immunohistochemical Study. International Journal of Morphology, 30, 829-833.
https://doi.org/10.4067/S0717-95022012000300010

[11]   Bhandari, M., Fong, K., Sprague, S., Williams, D. and Petrisor, B. (2012) Variability in the Definition and Perceived Causes of Delayed Unions and Nonunions. The Journal of Bone and Joint Surgery, 94, 1091-1096.
https://doi.org/10.2106/JBJS.K.01344

[12]   Berman, D., Oren, J.H., Bendo, J. and Spivak, J. (2017) The Effect of Smoking on Spinal Fusion. International Journal of Spine Surgery, 11, 229-238.
https://doi.org/10.14444/4029

[13]   National Research Council (2011) National Research Council Guide for the Care and Use of Laboratory Animals. 8th Edition, The National Academies Press, Washington DC.

[14]   Park, S.B., Lee, Y.J. and Chung, C.K. (2010) Bone Mineral Density Changes after Ovariectomy in Rats as an Osteopenic Model: Stepwise Description of Double Dorso-Lateral Approach. Korean Neurosurgery Society, 48, 309-312.
https://doi.org/10.3340/jkns.2010.48.4.309

[15]   Acar, Z., Deger, U., Görkem, Ü., Togrul, C., Deveci, E., Kaya, S. and Ekinci, S. (2016) Effects of Potentilla fulgens on Tuba Uterina in Ovariectomized Rats. International Journal of Morphology, 34, 460-464.
https://doi.org/10.4067/S0717-95022016000200008

[16]   Rizzoli, R., Bruyere, O., Cannata-Andia, J.B., et al. (2009) Management of Osteoporosis in the Elderly. Current Medical Research and Opinion, 25, 2373-2387.
https://doi.org/10.1185/03007990903169262

[17]   Watts, N.B., Lewiecki, E.M., Miller, P.D. and Baim, S. (2008) National Osteoporosis Foundation 2008 Clinician’s Guide to Prevention and Treatment of Osteoporosis and the World Health Organization Fracture Risk Assessment Tool (FRAX): What They Mean to the Bone Densitometrist and Bone Technologist. Journal of Clinical Densitometry, 11, 473-477.

[18]   Li, M., Shen, Y. and Wronski, T.J. (1997) Time Course of Femoral Neck Osteopenia in Ovariectomized Rats. Bone, 20, 55-61.
https://doi.org/10.1016/S8756-3282(96)00317-1

[19]   Wronski, T.J., Cintron, M. and Dann L.M. (1988) Temporal Relationship between Bone Loss and Increased Bone Turnover in Ovariectomized Rats. Calcified Tissue International, 43, 179-183.
https://doi.org/10.1007/BF02571317

[20]   Wronski, T.J., Dann, L.M. and Horner, S.L. (1989) Time Course of Vertebral Osteopenia in Ovariectomized Rats. Bone, 10, 295-301.
https://doi.org/10.1016/8756-3282(89)90067-7

[21]   Lelovas, P., Xanthos, T., Thoma, S., Lyritis, G. and Dontas, I. (2008) The Laboratory Rat as an Animal Model for Osteoporosis Research. Comparative Medicine, 58, 424-430.

[22]   Shen, V., Birchman, R., Liang, X.G., Wu, D.D., Lindsay, R. and Dempster D.W. (1997) Prednisolone Alone, or in Combination with Estrogen or Dietary Calcium Deficiency or Immobilization, Inhibits Bone Formation but Does Not Induce Bone Loss in Mature Rats. Bone, 21, 345-335.
https://doi.org/10.1016/S8756-3282(97)00153-1

[23]   Jee, W.S.S. and Yao, W. (2001) Overview: Animal Models of Osteopenia and Osteoporosis. Journal of Musculoskeletal and Neuronal Interactions, 1,193-207.

[24]   Turner, R.T., Lotinun, S., Hefferan, T., Evans, G.L., Zhang, M. and Sibonga, J.D. (2001) Animal Models for Osteoporosis. Reviews in Endocrine and Metabolic Disorders, 2, 117-127.
https://doi.org/10.1023/A:1010067326811

[25]   Danielsen, C., Mosekilde, L. and Svenstrup, B. (1993) Cortical Bone Mass, Composition, and Mechanical Properties in Female Rats in Relation to Age, Long-Term Ovariectomy, and Estrogen Substitution. Calcified Tissue International, 52, 26-33.
https://doi.org/10.1007/BF00675623

[26]   Wong, L.S., Green, H.M., Feugate, J.E., Yadav, M., Nothnagel, E.A. and Martins-Green, M. (2004) Effects of “Second-Hand” Smoke on Structure and Function of Fibroblasts, Cells that Are Critical for Tissue Repair and Remodeling. BMC Cell Biology, 5, 13.
https://doi.org/10.1186/1471-2121-5-13

[27]   Ma, L., Zwahlen, R.A., Zheng, L.W. and Sham, M.H. (2011) Influence of Nicotine on the Biological Activity of Rabbit Osteoblasts. Clinical Oral Implants Research, 22, 338-342.
https://doi.org/10.1111/j.1600-0501.2010.02088.x

 
 
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