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 JBM  Vol.7 No.10 , October 2019
Is Alpha-Glucosidase Inhibition a Mechanism of the Antidiabetic Action of Garlic (Allium sativum)?
Abstract: Objective: There has been a global surge in the number of diabetic cases. Many of the agents used as antidiabetic are either expensive or have side effects. Researchers are now turning their attention to phytotherapy as a viable alternative in the treatment of hyperglycemia. The aim of this study was to examine the inhibition of α-glucosidase as a possible mechanism of antidiabetic action of garlic. Method: The inhibitory effect of different concentrations of garlic was examined for alpha-glucosidase inhibitory activity in a 96-well micro plate. Saccharomyces cerevisiae was used as the source of alpha-glucosidase and the assay was analyzed with a Thermo Scientific® Multiskan Spectropho-meter at an absorbance of 400 nm. Result: The extracts of garlic exhibited a dose-dependent inhibition of alpha-glucosidase in comparison to acarbose. The IC50 of acarbose was 3.19 ± 0.42 mg/ml, for garlic, the IC50 was 16.93 mg/ml. Conclusion: In this study, garlic oil showed some promise as an antidiabetic agent with a mechanism of action similar to acarbose and miglitol that are currently used as antidiabetics. It is hoped that carrying out further research on garlic will elucidate other mechanisms of action.
Cite this paper: Obih, P. , Obih, J. and Arome, O. (2019) Is Alpha-Glucosidase Inhibition a Mechanism of the Antidiabetic Action of Garlic (Allium sativum)?. Journal of Biosciences and Medicines, 7, 42-49. doi: 10.4236/jbm.2019.710004.
References

[1]   Facts about Diabetes. World Health Organization.
https://www.who.int/features/factfiles/diabetes

[2]   Statistics about Diabetes. American Diabetes Association.
https://www.diabetes.org/resources/statistics/statistics-about-diabetes#sthash.F8fRkPqd.dpuf

[3]   American Diabetes Association.
https://www.diabetes.org/resources/statistics/statistics-about-diabetes#sthash.F8fRkPqd.dpuf
https://www.who.int/features/factfiles/diabetes/en


[4]   Nolte, M.S., Kennedy and Umesh Masharani (2018) Pancreatic Hormones & Antidiabetic Drugs; Bertram G. Katzung, Basic & Clinical Pharmacology, 14e.

[5]   Choudhury, H., Pandey, M., Hua, K., Mun, C., Jing, J., Kong, L., Liang Yee Ern, L., Ashraf, N., Kit, S., Yee, T., Pichika, M., Gorain, B. and Kesharwania, P. (2018) An Update on Natural Compounds in the Remedy of Diabetes Mellitus: A Systematic Review. Journal of Traditional and Complementary Medicine, 8, 361-376.
https://doi.org/10.1016/j.jtcme.2017.08.012

[6]   Harvey, A. (2010) Plant Natural Products in Anti-Diabetic Drug Discovery. Current Organic Chemistry, 14, 1670-1677.
https://doi.org/10.2174/138527210792927681

[7]   Lebovitz, H.E. (1997) A-Glucosidase Inhibitor. Endocrinology & Metabolism Clinics of North America, 26, 539-551.
https://doi.org/10.1016/S0889-8529(05)70266-8

[8]   Fugita, H., Yamagami, T. and Ohshima, K. (2001) Long-Term Ingestion of a Fermented Soybean-Derived Touchi Extract with Alpha-Glucosidase Inhibitory Activity Is Safe and Effective in Humans with Borderline and Mild Type-2 Diabetes. The Journal of Nutrition, 131, 1211.
https://doi.org/10.1093/jn/131.8.2105

[9]   Ramírez, G., Zavala, M., Pérez, J. and Zamilpa, A. (2012) In Vitro Screening of Medicinal Plants Used in Mexico as Antidiabetics with Glucosidase and Lipase Inhibitory Activities. Evidence-Based Complementary and Alternative Medicine, 2012, Article ID: 701261.
https://doi.org/10.1155/2012/701261

[10]   Wehmeier, U.F. and Piepersberg, W. (2004) Biotechnology and Molecular Biology of the α-Glucosidase Inhibitor Acarbose. Applied Microbiology and Biotechnology, 63, 613-625.
https://doi.org/10.1007/s00253-003-1477-2

[11]   Ríos, J.L., Francini, F. and Schinella, G.R. (2015) Natural Products for the Treatment of Type 2 Diabetes Mellitus. Planta Medica, 81, 975-994.
https://doi.org/10.1055/s-0035-1546131

[12]   Akshatha, V.J., Nalini, M.S., D'Souza, C. and Prakash, H.S. (2014) Streptomycete Endophytes from Anti-Diabetic Medicinal Plants of the Western Ghats Inhibit α-Amylase and Promote Glucose Uptake. Letters in Applied Microbiology, 58, 433-439.
https://doi.org/10.1111/lam.12209

[13]   Melzig, M.F. and Funke, I. (2007) Pflanzliche Alpha-Amylasehemmer eine Möglichkeit zur Phyto-therapie bei Diabetes Mellitus Typ II? Wiener Medizinische Wochenschrift, 157, 320-324.
https://doi.org/10.1007/s10354-007-0433-7

[14]   Rahimzadeh, M., Jahanshahi, S., Moein, S. and Moein, M.R. (2014) Evaluation of Alpha-Amylase Inhibition by Urtica dioica and Juglans regia Extracts. Iranian Journal of Basic Medical Sciences, 17, 465-469.

[15]   Bayan, L., Koulivand, P. and Gorji, A. (2014) Garlic: A Review of Potential Therapeutic Effects. Avicenna Journal of Phytomedicine, 4, 1-14.

[16]   Padiya, R. and Banerjee, S.K. (2013) Garlic as an Anti-Diabetic Agent: Recent Progress and Patent Reviews. Recent Patents on Food, Nutrition & Agriculture, 5, 105-127.
https://doi.org/10.2174/18761429113059990002

[17]   Ramirez, G., Zavala, M., Perez, J. and Zamilpa, A. (2012) In Vitro Screening of Medicinal Plants Used in Mexico as Antidiabetics with Glucosidase and Lipase Inhibitory Activity. Evidence-Based Complementary and Alternative Medicine, 2012, Article ID: 701261.
https://doi.org/10.1155/2012/701261

[18]   Wang, S., Li, M., Wang, X., Li, X., Yin, H., Jiang, L., Han, W., Irving, G., Zeng, T. and Xie, K. (2017) Diallyl Trisulfide Attenuated n-Hexane Induced Neurotoxicity in Rats by Modulating P450 Enzymes. Chemico-Biological Interactions, 265, 1-7.
https://doi.org/10.1016/j.cbi.2017.01.013

[19]   Lee, H.S., Lim, W.C., Lee, S.J., Lee, S.H., Lee, J.H. and Cho, H.Y. (2016) Anti-Obesity Effect of Garlic Extract Fermented by Lactobacillus Plantarum bl2 in Diet-Induced Obese Mice. Journal of Medicinal Food, 19, 823-829.
https://doi.org/10.1089/jmf.2016.3674

[20]   Eidi, A., Eidi, M. and Esmaeili, E. (2005) Antidiabetic Effect of Garlic (Allium sativum L.) in Normal and Streptozotocin-Induced Diabetic Rats. Phytomedicine, 13, 624-629.
https://doi.org/10.1016/j.phymed.2005.09.010

[21]   Poonam, T., Prakash, G.P. and Kumar, L.V. (2013) Influence of Allium sativum Extract on the Hypoglycemic Activity of Glibenclamide: An Approach to Possible Herb-Drug Interaction. Drug Metabolism and Drug Interactions, 28, 225-230.
https://doi.org/10.1515/dmdi-2013-0031

[22]   Shang, A., Cao, S.Y., Xu, X.Y., Gan, R.Y., Tang, G.Y., Corke, H., Mavumengwana, V. and Li, H.B. (2019) Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.). Foods, 8, pii: E246.
https://doi.org/10.3390/foods8070246

[23]   Wang, J., Zhang, X., Lan, H.H. and Wang, W. (2017) Effect of Garlic Supplement in the Management of Type 2 Diabetes Mellitus (T2DM): A Meta-Analysis of Randomized Controlled Trials. Food & Nutrition Research, 61, Article ID: 1377571.
https://doi.org/10.1080/16546628.2017.1377571

[24]   Ashraf, R., Aamir, K., Shaikh, A.R., et al. (2005) Effects of Garlic on Dyslipidemia in Patients with Type 2 Diabetes Mellitus. Journal of Ayub Medical College Abbottabad, 17, 60-64.

[25]   Sobenin, I.A., Nedosugova, L.V., Filatova, L.V., et al. (2008) Metabolic Effects of Time-Released Garlic Powder Tablets in Type 2 Diabetes Mellitus: The Results of Double-Blinded Placebo-Controlled Study. Acta Diabetologica, 45, 1-6.
https://doi.org/10.1007/s00592-007-0011-x

[26]   Ashraf, R., Khan, R.A. and Ashraf, I. (2011) Effects of Garlic on Blood Glucose Levels and HbA1c in Patients with Type 2 Diabetes Mellitus. Journal of Medicinal Plants Research, 5, 2922-2928.

[27]   Atkin, M., Laight, D. and Cummings, M.H. (2016) The Effects of Garlic Extract upon Endothelial Function, Vascular Inflammation, Oxidative Stress and Insulin Resistance in Adults with Type 2 Diabetes at High Cardiovascular Risk. A Pilot Double Blind Randomized Placebo Controlled Trial. Journal of Diabetes and its Complications, 30, 723-727.
https://doi.org/10.1016/j.jdiacomp.2016.01.003

[28]   Tsai, C.-W., Chen, H.-W., Sheen, L.-Y. and Lii, C.-K. (2012) Garlic: Health Benefits and Actions. BioMedicine, 2, 17-29.
https://doi.org/10.1016/j.biomed.2011.12.002

[29]   Hayat, S., Cheng, Z., Ahmad, H., Ali, M., Chen, X. and Wang, M. (2016) Garlic from Remedy to Stimulant: Evaluation of Antifungal Potential Reveals Diversity in Phytoalexin Allicin Content among Garlic Cultivars; Allicin Containing Aqueous Garlic Extracts Trigger Antioxidants. Frontiers in Plant Science, 7, 1235.
https://doi.org/10.3389/fpls.2016.01235

[30]   Percival, S.S. (2016) Aged Garlic Extract Modifies Human Immunity. The Journal of Nutrition, 146, 433S-436S.
https://doi.org/10.3945/jn.115.210427

 
 
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