GEP  Vol.7 No.10 , October 2019
Efficacy of Purple Non Sulphur Bacterium Rhodobacter sphaeroides Strain UMSFW1 in the Utilization of Palm Oil Mill Effluent
Abstract: Sustainable use of palm oil mill effluent (POME) has been the major focus in the recent development in palm oil industry due to the fact that environmental issue brought by POME. The purpose of this study was to determine the optimum incubation period of purple non-sulphur bacterium (PNSB) in reduction of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) in settled POME and to determine the dry cell weight, TN, TP and cell yield of PNSB. Pure isolate of Rhodobacter sphaeroides strain UMSFW1 was cultured in settled POME under anaerobic condition at 2500 lux illumination on light intensity at a temperature of 30°C ± 2°C for 144-h. Parameters such as COD (mg/L), dry cell biomass (g/L), TP (mg/L) and TN (mg/L) in settled POME and bacterial cells were analyzed. A total reduction of TN (43.9%) in settled POME and a total increase of TN (43.2%) in bacterial cell were recorded at the end of experiment. At the same time the reduction of 51.5% chemical oxygen demand was determined from the POME. The highest dry cell weight of 2.44 g/L with cell yield 0.39 (mg/cell/mg COD) was achieved at the end of experiment. A total 24.7% of TP reduction in settled POME was achieved in 144-h culture, but while a maximum 10% of TP in bacterial cell was achieved in 48-h culture. This study shows that PNSB Rhodobacter sphaeroides strain UMSFW1 grows well by using settled POME as substrate and is capable to remove TN in the settled POME and assimilate into bacterial biomass. This study could provide us a further insight in the nutrient removal and COD removal in the bioremediation process by bacterium Rhodobacter sphaeroides strain UMSFW1.
Cite this paper: Azad, S. , Chin, F. and Lal, M. (2019) Efficacy of Purple Non Sulphur Bacterium Rhodobacter sphaeroides Strain UMSFW1 in the Utilization of Palm Oil Mill Effluent. Journal of Geoscience and Environment Protection, 7, 1-12. doi: 10.4236/gep.2019.710001.

[1]   Ahmad, A. L., Ismail, S., & Bhatia, S. (2003). Water Recycling from Palm Oil Mill Effluent (POME) Using Membrane Technology. Desalination, 157, 87-95.

[2]   APHA (2005). Standard Methods for the Examination of Water and Wastewater (21st ed.). Washington DC: American Public Health Association, American Water Works Association and Water Environment Federation.

[3]   Azad, S. A., & Shaleh, S. R. M. (2015). Inoculum Sizes of Locally Isolated Phototrophic Bacterium on the Utilization of Palm Oil Mill Effluent. British Biotechnology Journal, 8, 1-11.

[4]   Azad, S. A., Soon, T. K., & Ransangan, J. (2013). Effects of Light Intensities and Photoperiods on the Growth and Proteolytic Activity in Purple Non-Sulfur Marine Bacterium Afifella marina Strain ME (KC205142). Advances in Biosciences and Biotechnology, 4, 919-924.

[5]   Azad, S. A., Vikineswary, S., Chong, V. C., & Ramachandran, K. B. (2003). Rhodovulum sulfidophilum in the Treatment and Utilization of Sardine Processing Wastewater. Letters in Applied Microbiology, 38, 13-18.

[6]   Azad, S. A., Vikineswary, S., Chong, V. C., & Ramachandran, K. B. (2001). Growth of Phototrophic Bacterium Rhodovulum sulfidophilum in Sardine Processing Wastewater. Letter in Applied Microbiology, 33, 264-268.

[7]   De Lima, L., Ponsano, E., & Pinto, M. (2011). Cultivation of Rubrivivax gelatinosus in Fish Industry Effluent for Depollution and Biomass Production. World Journal of Microbiology and Biotechnology, 27, 2553-2558.

[8]   Department of Environmental (DOE) (1999). Industrial Processes & the Environment: Crude Palm Oil Industry. Handbook No. 3. Kuala Lumpur: Aslita Sdn Bhd.

[9]   Hulsen, T., Batstone, D. J., & Keller, J. (2014). Phototrophic Bacteria for Nutrient Recovery from Domestic Wastewater. Water Research, 50, 18-26.

[10]   Kantachote, D., Torpee, S., & Umsakul, K. (2005). The Potential Use of Anoxygenic Phototrophic Bacteria for Treating Latex Rubber Sheet Wastewater. Journal of Biotechnology, 8, 314-323.

[11]   Khatipov, E., Miyake, M., Miyake, J., & Asada, Y. (1998). Accumulation of Poly-β-Hy- droxybutyrate by Rhodobacter sphaeroides on Various Carbon and Nitrogen Substrates. FEMS Microbiology Letters, 162, 39-45.

[12]   Kim, M. K., Choi, K. M., Yin, C. R., Lee, K. Y., Im, W. T., Lim, J. H., & Lee, S. T. (2004). Odorous Swine Wastewater Treatment by Purple Non-Sulfur Bacteria, Rhodopseudomonas palustris, Isolated from Eutrophicated Ponds. Biotechnology Letters, 26, 819-822.

[13]   Kobayashi, M., & Kobayashi, M. (1995). Waste Remediation and Treatment Using Anoxygenic Photosynthetic Bacteria. In R. E. Blankensh, M. T. Madigan, & C. E. Bauer (Eds.), Anoxygenic Photosynthetic Bacteria (pp. 1269-1282). Boston, London: Kluwer Academic Publisher.

[14]   Liang, M. C., Hung, C. H., Hsu, S. C., & Yeh, I. C. (2010). Purple Non-Sulphur Bacteria Diversity in Activated Sludge and Its Potential Phosphorus-Accumulating Ability under Different Cultivation Conditions. Applied Microbiology and Biotechnology, 86, 709-719.

[15]   Loo, P. L., Vikineswary, S., & Chong, V. C. (2013). Nutritional Value and Production of Three Species of Purple Non-Sulfur Bacteria Grown in Palm Oil Mill Effluent and Their Application in Rotifer Culture. Aquaculture Nutrition, 19, 895-907.

[16]   Merugu, R., Pratap Rudra, M. P., Girisham, S., & Reddy, S. M. (2012). Biotechnological Applications of Purple Non-Sulphur Phototrophic Bacteria: A Mini Review. International Journal of Applied Biology and Pharmaceutical Technology, 3, 376-384.

[17]   Middleburg, J. J., & Nieuwenhuize, J. (2000). Nitrogen Uptake by Heterotrophic Bacteria and Phytoplankton in the Nitrate-Rich Thames Estuary. Marine Ecology Progress Series, 203, 13-21.

[18]   Prasertsan, P., Jaturapornpipat, M., & Siripatana, C. (1997). Utilization and Treatment of Tuna Condensate by Photosynthetic Bacteria. Pure and Applied Chemistry, 69, 2439-2445.

[19]   Sasaki, K., & Nagai, S. (1979). The Optimum pH and Temperature for the Aerobic Growth of Rhodopseudomonas gelatinosa, and Vitamin B12 and Ubiquinone Formation on a Starch Medium. Journal of Fermentation Technology, 57, 383-386.

[20]   Sasaki, K., Noparatnaraporn, N., & Nagai, S. (1991). Use of Photosynthetic Bacteria for the Production of SCP and Chemicals from Agroindustrial Wastes. In A. M. Martin (Ed.), Bioconversion of Waste Materials to Industrial Products (pp. 225-264). London: Elsevier Applied Science.

[21]   Sawada, H., Parr, R. C., & Roger, P. L. (1977). Photosynthetic Bacteria in Wastewater Treatment. Journal of Fermentation Technology, 55, 326-336.

[22]   Soon, T. K., Azad, S. A., & Ransangan, J. (2013). Effect of Light Intensities and Photoperiod on Production of Extracellular Nucleic Acids in Purple Non-Sulfur Marine Bacterium Afifella marina Strain ME (KC205142). International Journal of Research in Pure and Applied Microbiology, 3, 53-57.

[23]   Suwansaard, M., Choorit, W., Zeilstra-Ryalls, J. H., & Prasertan, P. (2009). Isolation of Anoxygenic Photosynthetic Bacteria from Songkhla Lake for Use in a Two-Staged Biohydrogen Production Process from Palm Oil Mill Effluent. International Journal of Hydrogen Energy, 34, 7523-7529.

[24]   Tan, K. M., Liew, W. L., Muda, K., & Kassim, M. A. (2015). Microbiological Characteristic of Palm Oil Mill Effluent. In International Congress on Chemical, Biological, and Environmental Sciences (pp. 186-200). Kyoto.

[25]   Wei, H. Y. (2016). Application of Photosynthetic Bacteria: Biocontrol of Pathogenic Root Rot Fungus and Other Applications. Ph.D. Thesis, Kagoshima: University Kagoshima.