Pollinators provide an essential service to the food production and natural ecosystems, Apis mellifera is the main species used for pollination of agricultural and horticultural crops because their body parts are modified to effect pollination, visit a wide variety of flower types and are relatively abundant and manageable   . The pollination services offered by this species in North American crops were estimated at US $11.68 billion in the year 2009, similarly, on crops dependent in Brazil, the economic contribution of all pollinators corresponded to US $12 billion or 30% of the total production   . Interspecific interactions between A. mellifera and wild bees may modify the behaviour and increase the pollination value of individual species  . However, many studies have reported declines in bee populations around the world and have been the subject of intense research due to the ecological and economic damages resulting from the loss of pollination services     .
Bee pollination increases the quality and yield of many agricultural crops     , however, in Brazil, the beekeeping is mostly focused on the production of honey, propolis and pollen   . One of the great barriers of beekeeping is the cold and dry season, when the supply of floral resources in the environment decreases, causing a decrease in the development of the colonies  . Some of the alternatives for this period are the supplementation of the colonies  and the planting of melliferous flora   . Among the species used for flowering in the winter period is the Dombeya wallichii.
The genus Dombeya of the family Malvaceae has a paleotropical distribution with 206 species, of which 173 are endemic to the islands of Madagascar and Comoros  ; one of them, D. wallichii was introduced and adapted in Brazil, due to similar tropical conditions. It presents a shrub tree size of three to nine meters, forming large dense canopies with perennial life cycle, flowering period in fall/winter and flowering peak between June and July, with the influence of location and climatic conditions  .
The efficiency in angiosperm reproduction is related to strategies developed to attract pollinators  ; D. wallichii has floral characteristics such as colour, odour and nutritional rewards (nectar and pollen) to attract several floral visitors and thus to succeed in pollination   . Among these visitors, bees are the dominant group, mainly A. mellifera and Trigona spinipes, in addition to other insects of the Hymenoptera, Lepidoptera, Coleoptera, Hemiptera and Diptera order    .
Due to the lack of information on the interaction between the flower of D. wallichii and floral visitors, the present study evaluated the pollination behaviour of A. mellifera and Scaptotrigona bipunctata on D. wallichii, as well as the sugar concentration in the nectar along the day, in flower and in the honey crop of Africanized honeybee.
2. Material and Methods
The experiment was developed at the Experimental Farm of Iguatemi (Figure 1), State University of Maringá, Maringá-State of Paraná, in the South of Brazil,
Figure 1. Location of the study area.
where the climate is subtropical, with warm, humid, mesothermal summer and the average temperature of the hottest month exceeding 22˚C, i.e., Cfa according to the Köppen classification  .
During the peak of flowering of D. wallichii, from 8 am to 5 pm, ten flowers and ten bees were collected to analyze the sugar concentration (˚Brix) in the nectar and in the honey crop, with a capillary tube (10 μL) and refractometer (Atago Refractometer). Also, the foraging behaviour of Apis mellifera and Scaptotrigona bipunctata was recorded, following the focal plant method (126.96.36.199) described by  in which were observed for 10 - 20 minutes per hour of pollinator activity his behaviour of a particular individual. For each visit, the following variables were observed: visitation rate; length of visit; type of floral resource collected (nectar or pollen) and if there was contact with anthers and stigma.
The floral constancy was evaluated in four times (8 and 11 am, 2 and 5 pm) by pollen analysis collected from the pollen basket  , staining the pollen grains with 1% acetic carmine and comparing their external morphology with the pollen grains collected directly on the anthers of the flower buds. 500 grains were counted under an optical microscope, and the percentage of pollen of D. wallichii and other floral species was calculated. In addition, the climatic variables, temperature (˚C), humidity (%) and solar radiation (100X Lux) were measured on 28 June, 4 and 9 July 2018 using a thermo hygrometer and a digital lux meter.
A. mellifera honeybees collecting nectar were more abundant (74.3%) than those collecting pollen (4.7%) and those collecting both resources (21.0%). At 4 pm, all A. mellifera individuals were collecting nectar and at 1 pm, 50% were collecting nectar and 50%, nectar and pollen (Figure 2(a)). In S. bipunctata, a higher number of bees were observed collecting nectar (69.3%) compared to pollen foragers (17.0%) and collecting both resources (13.7%). At 11 am, there was the highest percentage of nectar foragers (83.3%) and at 5 pm, 66.7% of the bees were collecting pollen (Figure 2(b)).
The sugar concentration in the nectar ranged from 9.7 to 15.7 ˚Brix, while the content of the honey crop presented higher values, from 12.2 to 16.3 ˚Brix; at 12 pm, the highest sugar concentration was observed in both the plant and the honey crop (Figure 2(c)). There was a positive interaction between temperature and luminosity, in which, in the period from 12 to 1 pm the peaks in the two variables were found, around 24˚C and 550 (100X Lux), respectively. The humidity was inversely proportional to temperature and luminosity (Figures 2(d)-(e)).
A. mellifera visited on average 4.2 flowers per minute with a length of 9.2 seconds in each flower, and S. bipunctata, 1.1 flowers per minute, with a length of 34.2 seconds per visit. However, the dynamics of S. bipunctata specimens with the reproductive structures of the flower is less effective for plant fertilization, since 42.0% of the bees collected the nectar without touching the anther or the stigma. On the other hand, A. mellifera came into contact with the two reproductive structures in 80% of visits (Table 1).
The A. mellifera and S. bipunctata presented high constancy for the flower of D. wallichii, since the percentages of pollen grains of this species contained in the bees’ pollen basket were 98.6% and 94.1%, respectively. In relation to the other plant species found in the pollen load, A. mellifera had a higher amount of pollen grains from Emilia sonchifolia and in S. bipunctata, pollen from Raphanus sativus L. After 10 am, we observed the highest constancy of A. mellifera, reaching 100%, while in S. bipunctata, it varied throughout the day from 50% to 100%.
Pollination by bees depends on climatic conditions, as they influence both the crop and the bees  , from 11 to 12 although there is an increase in sugar concentration in nectar, the data show a reduction in nectar collection, coinciding with temperature increase (22˚C - 23˚C), luminosity (632.5 - 662 100X lux) and relative humidity reduction (50% - 46%). However, the high temperature is not the only factor that interferes with the collection behaviour of the bees, so it is necessary to interact with the relative humidity and the luminosity. Given that at 3 to 4 pm, there is an increase in the nectar collected by bees, even with a decrease in sugar concentration in nectar, temperature, luminosity and increase in relative humidity (Figure 2). Temperature has a significant effect on foraging activity of pollinators, in which high temperatures favour the presence of floral visitors while high relative humidity suppresses activity  . Therefore, in colder periods there was less presence of floral visitors, this same behaviour was observed in two species of stingless bees, Melipona beecheii and Melipona fasciata  .
Figure 2. (a)—Foraging behaviour of Apis mellifera. (b)—Foraging behaviour of Scaptotrigona bipunctata. (c)—Sugar concentration in the nectar of the flower and in the honey crop of Apis mellifera. (d)-(e)—Climatic data of the evaluated period.
Table 1. Foraging behaviour of Apis mellifera and Scaptotrigona bipunctata on the flower of Dombeya wallichii.
Regardless of the time, A. mellifera and S. bipunctata collected mainly nectar (74.3% and 69.3%, respectively), with a lower frequency of pollen harvesting (4.7% and 17.0%) or both resources (21.0% and 13.7%). Similar data were observed in canola pollination, in which A. mellifera collected mainly nectar (67.7% - 90.0%), followed by pollen (3.0% and 11.7%) and nectar and pollen (7.0% and 20.6%)   . In Tetragonisca angustula, higher percentages were observed (74.3% nectar, 24.8 pollen and 0.4% of both resources), thus corroborating the results obtained in this study. These changes in the collection behaviour may be related to the supply of available pollen, the reduction of pollen and nectar hoarding and the consumption of the colony, occurring a greater demand and therefore a greater number of foragers when the storage is low or when the energy needs of the colony increase   .
During the evaluation period, the A. mellifera and S. bipunctata were constant on the flowers of D. wallichii, and only a small part of the pollen analyzed (1.4% and 6.0% respectively) does not belong to the species under study. Forager bees visit other flowers to follow the changes in rewards over time; their preference is altered in response to a sequence of low rewards or reduced availability of the preferred flower  . A. mellifera visited on average 4.2 flowers per minute, with a length of 9.2 seconds per flower while S. bipunctata visited 1.1 flowers with a length of 34.2 seconds per flower. An effective pollinator sequentially visits the flowers, transporting and transferring the pollen to the stigma during the visit   , A. mellifera was considered a pollinator because it visited a greater number of flowers per minute and in only 3.0% of the visits it had no contact with the reproductive structures of the plant.
Chambo et al.  analyzed pollination of canola and observed a visitation rate of 12.9, with a length of 4.2 seconds in each flower for A. mellifera, also reporting, that Trigona spinipes bee spend more time visiting the flower (36.0 s). However, in Momordi cacharantia (Cucurbitaceae) the visitation rate of A. mellifera is 2.2 with a length of 5.2 seconds per flower and for the genus Trigona sp., the visitation rate is 5.4 with 23.2 seconds per flower  . Thus, the visitation rate and the time of permanence of the bees vary depending on the biology and floral morphology of each plant and the species of visiting bees, usually stingless bees stay longer visiting the flower.
The distribution of floral visitors on the plant will depend on the age of the flower, because it interferes with the nectar volume and the sugar concentration  . This concentration is influenced by several factors, including environmental conditions  . Thus, high temperatures contribute to high sugar concentrations in the nectar secreted by the plant  . However, when the sugar concentration in the nectar decreases, visitors continue to attend the flower, since the D. wallichii nectar contains lipid and phenolic substances, protein compounds and acidic and neutral polysaccharides that are nutritive for bees  .
In this study, we observed that the sugar concentration in the nectar of D. wallichii was not constant throughout the day, with a positive relationship with temperature and luminosity, this characteristic is considered an evolutionary strategy of plants to attract the insect pollinator for visiting other flowers  . Likewise, the sugar concentration in the honey crop varied with environmental conditions, agreeing with  who reported a positive correlation of sugar concentration in the honey crop with temperature for Melipona ferruginea and Hypotrigona gribodoi. Besides, the concentration of sugars is higher in the honey crop (14.2%) when compared to the concentration of nectar produced by the flower (12.3%), which is related to the behaviour of the worker bee that initiates the process of physical evaporation of the nectar and adds enzymes for conversion of sucrose into glucose and fructose  .
The foraging behaviour of the two bee species was influenced by temperature, relative humidity and luminosity. Both species had a preference for collecting nectar throughout the day, and in the hottest times of the day, the bees collected both resources (pollen and nectar). A. mellifera is a potential pollinator because it has visited more flowers per minute, touching the two reproductive structures of the flower. However, S. bipunctata was considered like floral visitor because, even staying more time in each flower, it collects the nectar without touching the reproductive structures. Finally, the sugar concentration was higher in the honey crop of the bee than in the flower, with higher concentration in times with the highest temperatures.
We thank Coordination of Improvement of Higher Level Personnel (CAPES) and National Council for Scientific and Technological Development (CNPq), process number 311663/2014-1for financial support.
 Abrol, D.P. (2012) Honeybee and Crop Pollination. In: Pollination Biology: Biodiversity Conservation and Agricultural Production, Springer Science & Business Media, Berlin, Heidelberg, 85-110.
 Calderone, N.W. (2012) Insect Pollinated Crops. Insect Pollinators and US Agriculture: Trend Analysis of Aggregate Data for the Period 1992-2009. PLoS ONE, 7, e37235.
 Giannini, T.C., Cordeiro, G.D., Freitas, B.M., Saraiva, A.M. and Imperatriz-Fonseca, V.L. (2015) The Dependence of Crops for Pollinators and the Economic Value of Pollination in Brazil. Journal of Economic Entomology, 108, 849-857.
 Brittain, C., Williams, N., Kremen, C. and Klein, A.M. (2013) Synergistic Effects of Non-Apis Bees and Honey Bees for Pollination Services. Proceedings of the Royal Society B: Biological Sciences, 280, Article ID: 20212767.
 Costa-Maia, F.M., Lourenço, D.A.L. and Toledo, V.A.A. (2010) Aspectos Econômicos e Sustentáveis da Polinização por Abelhas. In: Newton Martin, T., Jaquiel Waclawovsky, A., Kuss, F., Signor Mendes, A. and José Brun, E., Eds., Sistemas de Produção Agropecuaria (Ciências Agrárias, Animais e Florestais), Editora UTFPR, Florianópolis, 45-67.
 Potts, S.G., Biesmeijer, J.C., Kremen, C., Neumann, P., Schweiger, O. and Kunin, W.E. (2010) Global Pollinator Declines, Trends, Impacts and Drivers. Trends Ecology & Evolution, 25, 345-353.
 Zee, V.D.R., Pisa, L., Andonov, S., Brodschneider, R., Charrière, J.D., Chlebo, R., Coffey, M.F., Crailsheim, K., Dahle, B., Gajda, A., Wilkins, S., et al. (2012) Managed Honey Bee Colony Losses in Canada, China, Europe, Israel and Turkey, for the Winters of 2008-9 and 2009-10. Journal of Apicultural Research, 51, 100-114.
 Kulhanek, K., Steinhauer, N., Rennich, K., Caron, D.W., Sagili, R.R., Pettis, J.F., Ellis, J.D., Wilson, M.E., Wilkes, J.T., Tarpy, D.R., Rose, R., Lee, K., Rangel, J. and vanEngelsdorp, D. (2017) A National Survey of Managed Honey Bee 2015-2016 Annual Colony Losses in the USA. Journal of Apicultural Research, 56, 328-340.
 Chiari, W.C., Toledo, V.A.A., Ruvolo-Takasusuki, M.C.C., Oliveira, A.J.B., Sakaguti, E.S., Attencia, V.M., Costa, F.M. and Mitsui, M.H. (2005) Pollination of Soybean (Glycine max L. Merril) by Honeybees (Apis mellifera L.). Brazilian Archives of Biology and Technology, 48, 31-36.
 Chambó, E.D., Oliveira, N.T.E., Garcia, R.C., Ruvolo-Takasusuki, M.C.C. and Toledo, V.A.A. (2014) Pollination of Rapeseed (Brassica napus) by Africanized Honeybees (Hymenoptera: Apidae) on Two Sowing Dates. Anais da Academia Brasileira de Ciências, 86, 2087-2100.
 Chambó, E.D., Camargo, S.C., Garcia, R.C., Carvalho, C.A.L., Ruvolo-Takasusuki, M.C.C., Ronqui, L., Junior, C.S., Santos, P.R. and Toledo, V.A.A. (2018) Benefits of Entomophile Pollination in Crops of Brassica napus and Aspects of Plant Floral Biology. In: Brassica Germplasm-Characterization, Breeding and Utilization, IntechOpen, London, 95-106.
 Camargo, S.C., Garcia, R.C., Feiden, A., Vasconcelos, E.S., Pires, B.G., Hartleben, A.M., Moraes, F.J., Oliveira, l., Giasson, J., Mittanck, E.S., Gremaschi, J.R. and Pereira, D.J. (2014) Implementation of a Geographic Information System (GIS) for the Planning of Beekeeping in the West Region of Paraná. Anais da Academia Brasileira de Ciências, 86, 955-971.
 Araújo, K.S.S., Araújo, B. M., Viana, D.C., Honorato, J., Hunaldo, V.K.L., Cardoso, I.R.M., Neto dos Santos, D.L., de Oliveira, L.M., Pacheco, E., Dias, C.L., Nascimento, I., Silva, D.S., Salgado, G., Costa, J.R.M., Lobato, J.S.M. and Mariano, S.M.B. (2018) Beekeeping in Brazil: A Bibliographic Review. In: Apiculture, IntechOpen, London, 1-14.
 Vidal, M., Santana, N. and Vidal, D. (2008) Apicultural Flora and Apiary Management in the Reconcavo—South Bahia Region. Revista Acadêmica: Ciências Agrárias e Ambientais, 6, 503-509.
 Sereia, M.J. and Toledo, V.A.A. (2013) Quality of Royal Jelly Produced by Africanized Honeybees Fed a Supplemented Diet. Ciência e Tecnologia de Alimentos, 33, 304-309.
 Kovács-Hostyánszki, A., Földesi, R., Báldi, A., Endrédi, A. and Jordán, F. (2019) The Vulnerability of Plant-Pollinator Communities to Honeybee Decline: A Comparative Network Analysis in Different Habitat Types. Ecological Indicators, 97, 35-50.
 Sattler, D., Raedig, C., Hebner, A. and Wesenberg, J. (2019) Use of Native Plant Species for Ecological Restoration and Rehabilitation Measures in Southeast Brazil. In: Nehren, U., Schlter, S., Raedig, C., Sattler, D. and Hissa, H., Eds., Strategies and Tools for a Sustainable Rural Rio de Janeiro, Springer, Cham, 191-204.
 Toledo, V.A.A., Fritzen, A.E.T., Neves, C.A., Ruvolo-Takasusuki, M.C.C., Silva, H.S. and Terada, Y. (2003) Plants and Pollinating Bees in Maringá, State of Paraná, Brazil. Brazilian Archives of Biology and Technology, 46, 705-710.
 Barbosa, B.C., Paschoalini, M., Maciel, T.T. and Prezoto, F. (2016) Floral Visitors and Their Temporal Patterns of Activity in Flowers of Dombeya wallichii (Lindl.) K. Schum (Malvaceae). Entomotropica, 31, 131-136.
 Crislei, D. (2004) Estudo da Degradação dos Solos da Fazenda Experimental de Iguatemi (UEM) Distrito de Iguatemi-Maringá-PR. Mestrado em geografia, Dissertação, Faculdade de Geografia, Universidade Estadual de Maringá, Maringá.
 Chambó, E.D. (2013) Biologia Floral e Polinização em Canola (Brassica napus L.) Por Abelhas Africanizadas (Hymenoptera: Apidae) em Duas épocas de Semeadura. Ph.D. Thesis, University State of Maringá, Maringá.
 Gautam, P.P. and Kumar, N. (2018) Pollinator Diversity and Relative Abundance of Insect Pollinators on Ridge Gourd (Luffa acutangula) Flowers in Bihar (India). Journal of Entomology and Zoology Studies, 6, 1177-1181.
 Biesmeijer, J.C., Richter, J.A.P., Smeets, M.A.J.P. and Sommeijer, M.J. (1999) Niche Differentiation in Nectar-Collecting Stingless Bees: The Influence of Morphology, Floral Choice and Interference Competition. Ecological Entomology, 24, 380-388.
 Lima, E.G., Camargo, S.C., da Rosa Santos, P., Oliveira, J.W.S. and Toledo, V.A.A. (2016) Regulation of Pollen Foraging Activity in Apis mellifera Africanized Honeybees Colonies. Agricultural Sciences, 7, 335-340.
 Konzman, S. and Lunau, K. (2014) Divergent Rules for Pollen and Nectar Foraging Bumblebees—A Laboratory Study with Artificial Flower as Offering Diluted Nectar Substitute and Pollen Surrogate. PLoS ONE, 9, e91900.
 Adler, L.S. and Irwin, R.E. (2005) Comparison of Pollen Transfer Dynamics by Multiple Floral Visitors: Experiments with Pollen and Fluorescent Dye. Annals of Botany, 97, 141-150.
 Wang, S., Fu, W.L., Du, W., Zhang, Q., Li, Y., Lyu, Y.S. and Wang, X.F. (2018) Nectary Tracks as Pollinator Manipulators: The Pollination Ecology of Swertia bimaculata (Gentianaceae). Ecology and Evolution, 8, 3187-3207.
 Farkas, A., Molnár, R. Morschhauser, T. and Hahn, I. (2011) Variation in Nectar Volume e Sugar Concentration of Allium ursinum L. ssp. Ucrainicum in Three Habitats. The Scientific World Journal, 2012, Article ID: 138579.
 Dmitruk, M., Weryszko-Chmielewska, E. and Sulborska, A. (2018) Flowering and Nectar Secretion in Two Forms of the Moldavian Dragonhead (Dracocephalum moldavica L.)-A Plant with Extraordinary Apicultural Potential. Journal of Apicultural Science, 62, 97-110.
 Kajobe, R. (2007) Botanical Sources and Sugar Concentration of the Nectar Collected by Two Stingless Bee Species in a Tropical African Rain Forest. Apidologie, 38, 110-121.