AJPS  Vol.10 No.8 , August 2019
Potential Technological Use of Reserves of Jatropha curcas and J. macrocarpa Griseb. Seeds
Abstract: J. curcas and J. macrocarpa are useful for restoring degraded areas and their seeds contain oils for biodiesel production. The aim of the work was to determine the reserve substances in the endosperm and the embryo of J. curcas and J. macrocarpa which is important in understanding the germination process, the establishment of these species and its industrial employment. Seeds were imbibed in distilled water for 24 h, to facilitate removal of seed coat with the aim to separate the embryo and nutritive tissues. In both species, the endosperm contained aleurone grains consisting of a crystalloid and globoid, lipids of red color and the starch was not observed. Four major fatty acids were determined in J. curcas seed: oleic, palmitic, stearic, palmitoleic and oleic fatty acid represents about 70% oil content. Oleic acid was the most abundant in J. macrocarpa seeds, while, there was not palmitoleic acid. Seed with predominantly unsaturated fatty acids is ideal for biodiesel industry. The means of the sugar content were: 14.3 μg/mg in endosperm and 104.76 μg/mg in embryo of J. curcas and 6.48 μg/mg in endosperm and 59.20 μg/mg in embryo of J. macrocarpa. The means of the protein content were: 4.2 μg/mg in endosperm and 45.02 μg/mg in embryo of J. curcas and 3.26 μg/mg in endosperm and 31.08 μg/mg in embryo of J. macrocarpa. Sugar and protein contents of Jatropha seeds were significantly higher in embryo in both species (p < 0.1), which suggests early mobilization towards the embryo during imbibition period.
Cite this paper: de las M. Tavecchio, N. , Dardanelli, M. , Reguera, Y. , Reinoso, H. , Terenti, O. , Garbero, M. and Pedranzani, H. (2019) Potential Technological Use of Reserves of Jatropha curcas and J. macrocarpa Griseb. Seeds. American Journal of Plant Sciences, 10, 1444-1456. doi: 10.4236/ajps.2019.108102.

[1]   Wassner, D., Larran, A. and Rondanini, D. (2012) Evaluation of Jatropha macrocarpa as Oil Crop for Biodiesel Production in Arid Lands in the Dry Chaco, Argentina. Journal of Arid Environments, 77, 153-156.

[2]   Achten, W.M., Verchot, L., Franken, Y.J., Mathijs, E., Singh, V.P., Aerts, R. and Muys, B. (2008) Jatropha Bio-Diesel Production and Use. Biomass and Bioenergy, 32, 1063-1084.

[3]   Costa, B.J. and Oliveira, S.M.M. (2006) Biodiesel Production. Instituto de tecnologia do Paraná.

[4]   Arruda, F.P. (2004) Pinhao Manso (Jatropha curcas L.) Cultivation as an Alternative to the Northeastern Semiarid. Revista brasileira de oleaginosas e fibrosas, 8, 789-799.

[5]   Graham, I.A. (2008) Seed Storage Oil Mobilization. Annual Review of Plant Biology, 59, 115-142.

[6]   Suda, C.N.K. and Giorgini, J.F. (2000) Seed Reserve Composition and Mobilization during Germination and Initial Seedling Development. Revista Brasileira de Fisiologia Vegetal, 12, 226-245.

[7]   Souza, A.D.V., Fávaro, S.P., ítavo, L.C.V. and Roscoe, R. (2009) Chemical Characterization of Pinhao Manso, Nabo-Forrageiro and Crambre Seeds. Pesquisa Agropecuária Brasileira, 44, 1328-1335.

[8]   Souza Lopes, L., Gallao, M.I. and Campos, C.H. (2013) Mobilisation of Reserves during Germination of Jatropha Seeds. Revista Ciência Agronomica, 44, 371-378.

[9]   Johansen, D.A. (1940) Plant Microtechnique. McGraw-Hill, New York, London, 523.

[10]   D’Ambrogio de Argüeso, A. (1986) Technological Manual on Plant Histology. Hemisferio Sur. S.A., Buenos Aires, 54-59.

[11]   Bligh, E.G. and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917.

[12]   Morrison, W.R. and Smith, L.M. (1964) Preparation of Fatty Acid Methyl Esters and Dimethylacetals from Lipids with Boron Fluoride-Methanol. Journal of Lipid Research, 5, 600-608.

[13]   Kates, M. (1973) Techniques of Lipidology. North Holland Publishing Company, Amsterdam, London, 465-469.

[14]   DuBois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28, 350-356.

[15]   Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72, 248-254.

[16]   Bewley, J.D. (2013) Seeds, Physiology of Development, Germination and Dormancy. 3th Edition, Springer, New York, 392 p.

[17]   Tavecchio, N., Reinoso, H., Rufini Castiglione, M., Spanò, C. and Pedranzani, H.E. (2016) Anatomical Studies of Two Jatropha Species with Importance for Biodiesel Production. Journal of Agricultural Science, 8, 84-94.

[18]   Texeira, S.P. and Machado, S.R. (2008) Storage Sites in Seeds of Caesalpinia echinata and C. ferrea (Leguminosae) with Considerations on Nutrients Flow. Brasilian Archives of Biology and Technology, 51, 127-136.

[19]   Ahmad, S. and Sultan, S.M. (2015) Physiological Changes in the Seeds of Jatropha curcas L.) at Different Stages of Fruit Maturity. Brazilian Archives of Biology and Technology, 58, 118-123.

[20]   Oliveira Paula, S., Alencar de Sousa, J., Sousa de Brito, E. and Gallno, M.I. (2016) The Morphological Characterization of the Dry Seeds and Reserve Mobilization during Germination in Morinda citrifolia L. Revista Ciência Agronomica, 47, 556-563.

[21]   Casotti, W. (2008) Jatropha. Perspectives for Argentine Republic. Atesia, Argentina.

[22]   Pramanik, K. (2003) Properties and Use of Jatropha curcas Oil and Diesel Fuel Blends in Compression Ignition Engine. International Journal of Renewable Energy, 28, 239-248.

[23]   Nahar, K. and Sunny, S.A. (2016) Biodiesel, Glycerin and Seed-Cake Production from Roof-Top Gardening of Jatropha curcas L. Current Environmental Engineering, 3, 18-31.

[24]   Linder, C.R. (2000) Adaptive Evolution of Seed Oils in Plants: Accounting for the Biogeographic Distribution of Saturated and Unsaturated Fatty Acids in Seed Oils. The American Naturalist, 156, 442-458.

[25]   Ovando-Medina, I., Salvador-Figueroa, M. and Adriano-Anaya, M.L. (2015) Evolutionary Role of Seed Oils in Plants: The Case of Jatropha curcas L. Scientific Research and Essays, 10, 544-548.

[26]   Chaitanya, B.S.K, Kumar, S., Anjaneyulu, E., Prasad, R.B.N., Sastry, P.S. and Reddy, A.R. (2015) Pivotal Role of Sugar Fluxes between the Inner Integument and Endosperm in Lipid Synthesis during Seed Ontogeny in Jatropha curcas L. Industrial Crops and Products, 76, 1106-1113.

[27]   Pritchard, S.L., Charlton, W.L. and Graham, I.A. (2002) Germination and Storage Reserve Mobilization Are Regulated Independently in Arabidopsis. The Plant Journal, 31, 639-647.

[28]   Liu, Y., Sun, J., Tian, Z., Hakeen, A., Wang, F., Jiang, D., Cao, W., Adkins, S.W. and Dai, T. (2017) Physiological Responses of Wheat (Triticum aestivum L.) Germination to Elevated Ammonium Concentrations: Reserve Mobilization, Sugar Utilization, and Antioxidant Metabolism. Plant Growth Regulation, 81, 209-220.

[29]   Shah, M., Soares, E.L., Carvalho, P.C., Soares, A.A., Domont, G.B., Nogueira, F.C.S. and Campos, F.A.P. (2015) Proteomic Analysis of the Endosperm Ontogeny of Jatropha curcas L. Seeds. Journal of Proteome Research, 14, 2557-2568.

[30]   Ekman, A. (2008) Carbon Partitioning between Oil and Carbohydrates in Developing Oat (Avena sativa L.) Seeds. Journal of Experimental Botany, 59, 4247-4257.