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 JACEN  Vol.8 No.3 , August 2019
Iodine Fortification Study of Some Common African Vegetables
Abstract: Iodine Deficiency Diseases (IDDs) occupy important positions in the health problems of developing countries. Salt Iodisation has been the common approach to solving these problems. However, apart from the problems of lack of compliance by salt manufacturers, and inculturation of the consumers, health conditions aggravated by high salt intake by humans have become increasingly relevant. These problems can be eliminated if the commonly produced and consumed plants are fortified with Iodine. The prospects are in the inclusion of Iodine-containing compounds in the inorganic fertilizers used by farmers. In this study, Potassium Iodide and Potassium Iodate were used as inoculants. Five different concentrations0.1 M, 0.2 M, 0.3 M, 0.4 M, and 0.5 M of Potassium Iodide and Potassium Iodate solutions were used to inoculate the soils on which the following edible African plants were planted: Murraya koenigii; Ocimum gratissimum; Cucurbita pepo; Solanum nigrum; Amaranthus hybridus and Abelmoschus esclentus, Corchorous olitoruis, Solanum lycopersicum, Zingiber officinale, Telfairia occidentalis, Talinium triangulare, Solanum melongena. Controls were also planted. After 14 days, alkaline dry ash method was used to determine the Iodine concentrations in the plants. The results showed that Murraya koenigii showed the highest absorption of Iodine 6.90 mg/kg at 0.3 M using KI, followed by Amaranthus hybridus 6.40 mg/kg at 0.1 M. Solanum nigrum, Ocimum gratissimum and Zingiber officinale also showed good absorption. Other plants except Murraya koenigii, Ocimum gratissimum, Solanum nigrum and Zingiber officinale showed very low tolerance to KI absorption. The result also showed that Telfairia occidentalis showed the highest absorption of iodine 8.20 mg/kg at 0.2 M of KIO3 followed by Cucurbita pepo 6.40 mg/kg at also 0.2 M of KIO3. Murraya koenigii, Ocimum gratissimum, Solanum nigrum, Zingiber officinale also showed good absorption of KIO3. Some of the plants were not able to tolerate the absorption at higher concentration for both KI and KIO3. All the plants were poisoned at concentration of 0.5 M for both Ki and KIO3. Murraya koenigii, Ocimum gratissimum, Solanum nigrum, Zingiber officinale can be used in iodine biofortification using KI and KIO3 at concentration < 0.5 M. The overall result may be very significant, when it is considered that Iodine is a micronutrient, with a daily intake requirement of 100 - 150 μg/kg. It can be seen that there is hope in achieving this kind of biofortification.
Cite this paper: Anarado, C. , Anarado, C. , Areh, R. , Ifoh, N. , Eze, E. and Ikeakor, E. (2019) Iodine Fortification Study of Some Common African Vegetables. Journal of Agricultural Chemistry and Environment, 8, 172-183. doi: 10.4236/jacen.2019.83014.
References

[1]   Olum, S., Gellynck, X., Okello, C., Webale, D., Odongo, W., Ongeng, D. and De Steur, H. (2018) Stakeholders’ Perceptions of Agronomic Iodine Biofortification: A SWOT-AHP Analysis in Northern Uganda. Nutrients, 10, 407.
https://doi.org/10.3390/nu10040407

[2]   Winkler, J.T. (2011) Biofortification: Improving the Nutritional Quality of Staple Crops. 100-112.

[3]   Zimmermann, M.B., Jooste, P.L. and Pandav, C.S. (2008) Iodine-Deficiency Disorders. The Lancet, 372, 1251-1262.
https://doi.org/10.1016/S0140-6736(08)61005-3

[4]   Kiferle, C., Gonzali, S. and Holwerda, H.T. (2013) Tomato Fruits: A Good Target for Iodine Biofortification. Frontiers in Plant Science, 27, 205.
https://doi.org/10.3389/fpls.2013.00205

[5]   Tonacchera, M., Dimida, A., De Servi, M., Frigeri, M., Ferrarini, E., De Marco, G., Grasso, L., Agretti, P., Piaggi, P., Aghini-Lombardi, F., Perata, P., Pinchera, A. and Vitti, P. (2013) Iodine Fortification of Vegetables Improves Human Iodine Nutrition: In Vivo Evidence for a New Model of Iodine Prophylaxis. The Journal of Clinical Endocrinology & Metabolism, 98, E694-E697.
https://doi.org/10.1210/jc.2012-3509

[6]   Weng, H.X., Hong, C.L., Xia, T.H., et al. (2013) Iodine Biofortification of Vegetable Plants—An Innovative Method for Iodine Supplementation. Chinese Science Bulletin, 58, 2066-2072.
https://doi.org/10.1007/s11434-013-5709-2

[7]   Yaseen, M. (2013) Iodine Biofortification through Plant Biotechnology. Journal of Nutrition, 29, 1431.
https://doi.org/10.1016/j.nut.2013.04.009

[8]   Khalid, S.M.N. (2017) Iodine Bio-Fortification of Crops for Solving Iodine Deficiency Disorder Problem in Afghanistan. Journal of Food and Nutritional Disorders, 6, 3.
https://doi.org/10.4172/2324-9323.1000228

[9]   Signore, A., Renna, M., D’Imperio, M., Serio, F. and Santamaria, P. (2018) Preliminary Evidences of Biofortification with Iodine of “Carota di Polignano”: An Italian Carrot Landrace. Frontiers in Plant Science, 9, 170.
https://doi.org/10.3389/fpls.2018.00170

[10]   Zimmermann, M.B. (2008) Iodine Requirements and the Risks and Benefits of Correcting Iodine Deficiency in Populations. Journal of Trace Elements in Medicine and Biology, 22, 81-92.
https://doi.org/10.1016/j.jtemb.2008.03.001

[11]   Delange, F. (1994) The Disorders Induced by Iodine Deficiency. Thyroid, 4, 107-128.
https://doi.org/10.1089/thy.1994.4.107

[12]   Gonzali, S., Kiferle, C. and Perata, P. (2017) Iodine Biofortification of Crops: Agronomic Biofortification, Metabolic Engineering and Iodine Bioavailability. Current Opinion in Biotechnology, 44, 16-26.
https://doi.org/10.1016/j.copbio.2016.10.004

[13]   Lyons, G. (2018) Biofortification of Cereals with Foliar Selenium and Iodine Could Reduce Hypothyroidism. Frontiers in Plant Science, 9, 730.
https://doi.org/10.3389/fpls.2018.00730

[14]   Philip, J.W. and Martin, R.B. (2009) Biofortification of Crops with Seven Mineral Elements Often Lacking in Human Diets—Iron, Zinc, Copper, Calcium, Magnesium, Selenium and Iodine. New Phytologist, 182, 49-84.
https://doi.org/10.1111/j.1469-8137.2008.02738.x

[15]   Ujowundu, C.O., Ukoha, A.I., Agha, N.C., Nwachukwu, N. and Igwe, K.O. (2009) Iodine Biofortification of Selected Plants Using Potassium Iodide. Nigerian Journal of Biochemistry and Molecular Biology, 24, 17-21.

[16]   Landini, M., Gonzali, S. and Perata, P. (2011) Iodine Biofortification in Tomato. Journal of Plant Nutrition and Soil Science, 174, 480-486.
https://doi.org/10.1002/jpln.201000395

[17]   Prasad, B.V.G., Mohanta, S., Rahaman, S. and Prerna, B. (2015) Bio-Fortification in Horticultural Crops. Journal of Agricultural Engineering and Food Technology, 2, 95-99.

[18]   Rosell, C.M. (2016) Fortification of Grain-Based Foods. Reference Module in Food Science. Elsevier, Amsterdam.
https://doi.org/10.1016/B978-0-08-100596-5.00074-3

[19]   Montalvo, D., Degryse, F., da Silva, R.C., Baird, R. and McLaughlin, M.J. (2016) Chapter Five-Agronomic Effectiveness of Zinc Sources as Micronutrient Fertilizer. Advances in Agronomy, 139, 215-267.
https://doi.org/10.1016/bs.agron.2016.05.004

[20]   Penelope, N., Howarth, E.B., Meenakshi, J.V. and Wolfgang, P. (2006) Symposium: Food Fortification in Developing Countries Biofortification of Staple Food Crops. American Society for Nutrition, Rockville, 1064-1067.
https://doi.org/10.1093/jn/136.4.1064

[21]   Amy, S., Meike, S.A., et al. (2016) Biofortification Techniques to Improve Food Security. Reference Module in Food Science. Elsevier, Amsterdam.
https://doi.org/10.1016/B978-0-08-100596-5.03078-X

[22]   Taylor, J.R.N. and Duodu, K.G. (2017) Sorghum and Millets: Grain-Quality Characteristics and Management of Quality Requirements. In: Wrigley, C., Batey, I. and Miskelly, D., Eds., Cereal Grains: Assessing and Managing Quality Woodhead Publishing Series in Food Science, Technology and Nutrition, 2nd Edition, Elsevier, Amsterdam, Chapter 13, 317-351.
https://doi.org/10.1016/B978-0-08-100719-8.00013-9

[23]   Howarth, E.B. and Amy, S. (2017) Improving Nutrition through Biofortification: A Review of Evidence from HarvestPlus, 2003 through 2016. Global Food Security, 12, 49-58.
https://doi.org/10.1016/j.gfs.2017.01.009

[24]   Anarado, C.E., Anarado, C.J.O., Okeke, M.O., Ezeh, C.E., Umedum, N.L. and Okafor, P.C. (2019) Leafy Vegetables as Potential Pathways to Heavy Metal Hazards. Journal of Agricultural Chemistry and Environment, 8, 23-32.
https://doi.org/10.4236/jacen.2019.81003

[25]   Prasan, R.B. (2012) Curry Leaf (Murraya koenigii) or Cure Leaf: Review of Its Curative Properties. Journal of Medical Nutrition and Nutraceuticals, 1, 92-97.
https://doi.org/10.4103/2278-019X.101295

[26]   Iwu, M.M. (1993) Handbook of African Medicinal Plants. URP Press, Bocarolon, 229.

[27]   Vera, K.M., Senka, M.P., Zuzana, P., Ljiljana, R. and Draginja, P. (2011) Phenolic Acids in Pumpkin (Cucurbita pepo L.) Seeds. In: Preedy, V.R., Watson, R.R. and Patel, V.B., Eds., Nuts and Seeds in Health and Disease Prevention, Elsevier, Amsterdam, 925-932.
https://doi.org/10.1016/B978-0-12-375688-6.10109-4

[28]   Mohamed Saleem, T.S., Madhusudhana Chetty, C., Ramkanth, S., Alagusundaram, M., Gnanaprakash, K., Thiruvengada Rajan, V.S. and Angalaparameswari, S. (2009) Solanum nigrum Linn.—A Review. Pharmacognosy Review, 3, 342-345.

[29]   Akubugwo, I.E., Obasi, N.L., Chinyere, G.C. and Ugbogu, A.E. (2007) Nutritional and Chemical Value of Amaranthus hybridus L. Leaves from Afikpo, Nigeria. African Journal of Biotechnology, 6, 2833-2839.
https://doi.org/10.5897/AJB2007.000-2452

[30]   Priya, S., Varun, C., Brahm, K.T., Shubhendra, S.C., Sobita, S., Bilal, S. and Abidi, A.B. (2014) N Overview on Okra (Abelmoschus esculentus) and It’s Importance as a Nutritive Vegetable in the World. International Journal of Pharmacy and Biological Sciences, 4, 227-233.

[31]   Habib, O.A., Léonard, E.A., et al. (2018) Ethnobotanical Knowledge of Jute (Corchorus olitorius L.) in Benin. European Journal of Medicinal Plants, 26, 1-11.
https://doi.org/10.9734/EJMP/2018/43897

[32]   Trivedi, M., Singh, R., Shukla, M. and Tiwari, R.K. (2016) GMO and Food Security. In: Omkar, Ed., Ecofriendly Pest Management for Food Security, Elsevier, Amsterdam, 703-726.
https://doi.org/10.1016/B978-0-12-803265-7.00023-3

[33]   Mbaveng, A.T. and Kuete, V. (2017) Zingiber officinale. In: Medicinal Spices and Vegetables from Africa, Elsevier, Amsterdam, 637-639.
https://doi.org/10.1016/B978-0-12-809286-6.00030-3

[34]   Ajayi, S.A., Berjak, P., Kioko, J.I., Dulloo, M.E. and Vodouhe, R.S. (2006) Responses of Fluted Pumpkin (Telfairia occidentalis Hook. f.; Cucurbitaceae) Seeds to Desiccation, Chilling and Hydrated Storage. South African Journal of Botany, 72, 544-550.
https://doi.org/10.1016/j.sajb.2006.02.001

[35]   Okoli, B.E. and Mgbeogu, C.M. (1983) Fluted Pumpkin, Telfairia occidentalis: West African Vegetable Crop. Economic Botany, 32, 145-149.
https://doi.org/10.1007/BF02858775

[36]   Aja, P.M., Okaka, A.N.C., Onu, P.N., Ibiam, U. and Urako, A.J. (2010) Phytochemical Composition of Talinum triangulare (Water Leaf) Leaves. Pakistan Journal of Nutrition, 9, 527-530.
https://doi.org/10.3923/pjn.2010.527.530

[37]   Knapp, S., Vorontsova, M.S. and Prohens, J. (2013) Wild Relatives of the Eggplant (Solanum melongena L.): Solanaceae, New Understanding of Species Names in a Complex Group. PLoS ONE, 8, e57039.
https://doi.org/10.1371/journal.pone.0057039

[38]   Hirakawa, H., Shirasawa, K., Miyatake, K., et al. (2014) Draft Genome Sequence of Eggplant (Solanum melongena L.): The Representative Solanum Species Indigenous to the Old World. DNA Research, 21, 649-660.
https://doi.org/10.1093/dnares/dsu027

[39]   Ranganathan, S. (1995) Iodised Salt Is Safe. Indian Journal of Public Health, 39, 164-171.

[40]   Shetty, A., Rao, C.R., Kamath, A., Sp, V. and Reddy, T.S.K. (2019) Goiter Prevalence and Interrelated Components from Coastal Karnataka. The Indian Journal of Pediatrics, 86, 159-164.
https://doi.org/10.1007/s12098-018-2757-2

[41]   Yadav, S., Gupta, S.K., Godbole, M.M., Jain, M., Singh, U., Pavithran, V.P., Boddula, R., Mishra, A., Shrivastava, A., Tandon, A., Ora, M., Chowhan, A., Shukla, M., Yadav, N., Babu, S., Dubey, M. and Awasthi, P.K. (2010) Persistence of Severe Iodine-Deficiency Disorders Despite Universal Salt Iodization in an Iodine-Deficient Area in Northern India. Public Health Nutrition, 13, 424-429.
https://doi.org/10.1017/S1368980009990280

[42]   Dat, T.Q., Giang, L.N.H., Bao, P.V. and Tuyen, N.T.H. (2019) Prevalence of Goiter among Children Aged 8-10 in Binh Dinh Province, Vietnam in 2016-2017. AIMS Public Health, 6, 184-194.
https://doi.org/10.3934/publichealth.2019.2.184

[43]   Delshad, H. and Azizi, F. (2017) Review of Iodine Nutrition in Iranian Population in the Past Quarter of Century. International Journal of Endocrinology and Metabolism, 15, e57758.
https://doi.org/10.5812/ijem.57758

[44]   Yusuf, H.K., Rahman, A.M., Chowdhury, F.P., Mohiduzzaman, M., Banu, C.P., Sattar, M.A. and Islam, M.N. (2008) Iodine Deficiency Disorders in Bangladesh, 2004-05: Ten Years of Iodized Salt Intervention Brings Remarkable Achievement in Lowering Goitre and Iodine Deficiency among Children and Women. Asia Pacific Journal of Clinical Nutrition, 17, 620-628.

[45]   Heydon, E.E., Thomson, C.D., Mann, J., Williams, S.M., Skeaff, S.A., Sherpa, K.T. and Heydon, J.L. (2009) Iodine Status in a Sherpa Community in a Village of the Khumbu Region of Nepal. Public Health Nutrition, 12, 1431-1436.
https://doi.org/10.1017/S1368980008004242

[46]   De Benoist, B. and Delange, F. (2002) Iodine Deficiency: Current Situation and Future Prospects. Sante, 12, 9-17.

[47]   Nyenwe, E.A. and Dagogo-Jack, S. (2009) Iodine Deficiency Disorders in the Iodine-Replete Environment. The American Journal of the Medical Sciences, 337, 37-40.
https://doi.org/10.1097/MAJ.0b013e31817baaf0

[48]   Pearce, E.N., Andersson, M. and Zimmermann, M.B. (2013) Global Iodine Nutrition: Where Do We Stand in 2013? Thyroid, 23, 523-528.
https://doi.org/10.1089/thy.2013.0128

[49]   Rossi, L. and Branca, F. (2003) Salt Iodisation and Public Health Campaigns to Eradicate Iodine Deficiency Disorders in Armenia. Public Health Nutrition, 6, 463-469.
https://doi.org/10.1079/PHN2003461

[50]   Saha, S., Abu, B.A.Z., Jamshidi-Naeini, Y., Mukherjee, U., Miller, M., Peng, L.L. and Oldewage-Theron, W. (2019) Is Iodine Deficiency Still a Problem in Sub-Saharan Africa? A Review. Proceedings of the Nutrition Society, 11, 1-13.
https://doi.org/10.1017/S0029665118002859

[51]   Izzeldin, S.H., Crawford, M.A. and Ghebremeskel, K. (2009) Salt Fortification with Iodine: Sudan Situation Analysis. Nutrition and Health, 20, 21-30.
https://doi.org/10.1177/026010600902000103

[52]   Chuot, C.C., Galukande, M., Ibingira, C., Kisa, N. and Fualal, J.O. (2014) Iodine Deficiency among Goiter Patients in Rural South Sudan. BMC Research Notes, 7, 751.
https://doi.org/10.1186/1756-0500-7-751

[53]   Mezgebu, Y., Mossie, A., Rajesh, P. and Beyene, G. (2012) Prevalence and Severity of Iodine Deficiency Disorder among Children 6-12 Years of Age in Shebe Senbo District, Jimma Zone, Southwest Ethiopia. Ethiopian Journal of Health Sciences, 22, 196-204.

[54]   Fereja, M., Gebremedhin, S., Gebreegziabher, T., Girma, M. and Stoecker, B.J. (2018) Prevalence of Iodine Deficiency and Associated Factors among Pregnant Women in Ada District, Oromia Region, Ethiopia: A Cross-Sectional Study. BMC Pregnancy Childbirth, 18, 257.
https://doi.org/10.1186/s12884-018-1905-z

[55]   Harika, R., Faber, M., Samuel, F., Kimiywe, J., Mulugeta, A. and Eilander, A.(2017) Micronutrient Status and Dietary Intake of Iron, Vitamin A, Iodine, Folate and Zinc in Women of Reproductive Age and Pregnant Women in Ethiopia, Kenya, Nigeria and South Africa: A Systematic Review of Data from 2005 to 2015. Nutrients, 9, pii: E1096.
https://doi.org/10.3390/nu9101096

[56]   Ogbera, A.O. and Kuku, S.F. (2011) Epidemiology of Thyroid Diseases in Africa. Indian Journal of Endocrinology and Metabolism, 15, 82-88.
https://doi.org/10.4103/2230-8210.83331

[57]   Mshelia, D.S., Bakari, A.A., Mubi, B.M., Ali, N., Musa, A.H., Gali, R.M. and Mamza, Y.P. (2016) Biochemical Pattern of Thyroid Disorders in Maiduguri, Northeastern Nigeria. World Journal of Medicine and medical Science Research, 4, 32-38.

[58]   Ogbera, A.O., Fasanmade, O. and Adediran, O. (2007) Pattern of Thyroid Disorders in the Southwestern Region of Nigeria. Ethnicity & Disease, 17, 327-330.

[59]   Salami, B.A., Odusan, O., Ebili, H.O. and Akintola, P.A. (2016) Spectrum and Prevalence of Thyroid Diseases Seen at a Tertiary Health Facility in Sagamu, South-West Nigeria. Nigerian Postgraduate Medical Journal, 23, 137-140.
https://doi.org/10.4103/1117-1936.190345
http://www.npmj.org/text.asp?2016/23/3/137/190345

[60]   Sarmugam, R. and Worsley, A. (2014) Current Levels of Salt Knowledge: A Review of the Literature. Nutrients, 6, 5534-5559.
https://doi.org/10.3390/nu6125534

 
 
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