Due to the population growth and the industrial development, world energy consumption considerably increases in the last decades. The use of fossil fuels is still the main energy sources. However, fossil fuels are not renewable energies, further, they are highly polluting, and their production tends to decrease during the next few decades  . Yet, the increased energy consumption based on fossil fuels affects our environment through the greater amounts of greenhouse gas emissions, the environmental pollution of water, air and soil, and the climate changes, which dramatically influence the quality of life and the health of people. Therefore, it is important to develop new sustainable energy supply systems that cover the increasing energy demand from renewable sources. Reducing greenhouse gas emissions through renewable energy production is of rising importance  .
Biogas, produced by anaerobic digestion of wastewater, organic waste, agricultural waste, industrial waste, and animal by-products is a potential source of renewable energy  .
Anaerobic digestion is a series of biological process in which microorganism breaks down biodegradable material in the absence of oxygen. It is a step by step process where the organic carbon is mainly converted to carbon dioxide and methane. Figure 1 shows the process of producing biogas divided into hydrolysis, acidogenesis, acetogenesis and methanogenesis  .
Biogas is mainly methane and carbon dioxide and small amounts of hydrogen sulfide. The gases methane, hydrogen, and carbon monoxide can be combusted or oxidized with oxygen. This energy release allows biogas to be used as a fuel  . It can also be used in a gas engine to convert the energy in the gas into electricity and heat and even for internal combustion engines. Biogas can be compressed, the same way natural gas is compressed. When it becomes bio-methane, biogas can be upgraded to natural gas standards  .
The rate of biogas production depends greatly on the temperature for digesters. Anaerobic digestion process generally occurs at three temperature ranges. These ranges are defined as psychrophilic (15˚C - 20˚C), mesophilic (30˚C - 40˚C) and thermophilic (50˚C - 60˚C).
During biological decomposition and conversion processes, the rate of chemical
Figure 1. Flow chart of anaerobic digestion process.
reaction increases with the increase in the surrounding temperature. However, there should not be rapid temperature fluctuations during the digestion process, due to that methanogenic bacteria are sensitive to temperature conditions. A sudden change in temperature will hamper the methanogenic bacteria resulting in decreased biogas production. The optimum temperature for anaerobic digestion and methanogenic bacteria is found to be between 30˚C to 40˚C  . The relative growth rate of methanogens under different temperature conditions is presented in Figure 2.
2. Aim of the Study
The main objective of this research is investigation of biogas production by decomposition cow dung is needed for the students’ level. It is also trusted worth saying that the cow dung is generally available in the Arab counties.
3. Components of the Experiment
1) Two plastic tanks 18 L. The first tanks for digester and another tank for fill water.
2) Graduated cylinder 2000 mL for measure volume biogas produce.
4) Rubber tube.
4. Experimental Set-Up
This research was made to a simple demonstration of biogas production by decomposition of cow dung. The digester made of plastic tank of 18 liters capacity was used for set-up. The experimental the set-up is shown in Figure 3. The full setup for this experimental was the connection of the digester to the water displacement setup for the gas collection. The water displacement method of gas collection is a method in which gas is allowed to replace water at equal volume of water displaced and this was used to determine the volume of gas produced daily. The mode of feeding used was a batch feeding (discontinued feeding). This
Figure 2. Relation between temperature and growth rate of methanogens.
Figure 3. The experimental setup.
simply means loading the digester at once and maintaining a closed environment throughout the retention period. The procedures taken during feeding of the digester are as follow:
1) 5 kg of the cow dung was weighed and 5 liters of water was mixed thoroughly with of the waste in the ratio of 1:1.
2) The mixture of the waste was poured into digester.
The digesters were operated at temperature in the mesophilic range with a hydraulic retention time of 30 days.
5. Measurement Methods
Biogas production from the digesters was measured daily by graduated cylinder. Measure pH was analytical and food laboratory at Omar Al-Mukhtar University by using pH meter.
6. Results and Discussion
This experiment was carried out using cow dung. The details of the experiment are illustrated in Table 1.
Figure 4 shows the volume biogas produced from cow dung within the retention period 30 days. For biogas produced in cow dung, biogas was not produced for the first 8 days since it takes more time for cow dung to decompose afterward which gas is being produced. This is forecast because non growth of methanogenic bacteria. This can also be traced to the fact that most cows feed on fibrous materials and microorganisms require a longer time to degrade fibrous materials. Production of gas from cow dung started on day 9 of the retention period by producing average biogas of 30 ml, thereafter increases to 100 ml on day 10 and reduces to 50 ml on day 12. At day 13, the biogas produced was 160 ml in which decreases back to 45 ml on the next day and increases thereafter until it reached the peak on day 22 with 340 ml biogas production after which it begins to reduce till the completion of the retention period.
Figure 5 illustrates the pH of cow dung within the 30 days retention period.
Figure 4. Volume of biogas against number of days.
Figure 5. pH of Slurry against number of days.
Table 1. The details of the experiment.
The pH for cow dung fluctuate from the first day to the tenth day between 5 and 7.4, after which begins to decrease gradually for the remaining days of the retention period. As it was observed in the first few days, the pH of cow dung decreases due to high volatile fatty acid. The gradual reduction explains the gradually change of stage of the production of biogas, from hydrolysis to acidogenesis in which the slurry become acidic and form substrate after which it produces biogas.
This research shows that biogas can be produced from cow dung through anaerobic digestion. This dung is continuously offered in our location and can be used as an alternative energy resource. The research exposed further that cow dung as animal waste has large possibilities for production of biogas. The application should be encouraged due to high volume of biogas produces.
In the future, an experiment would be expanded for including all types of animal waste.
The researches would like to thank the Department of Sustainable and Renewable Energy Engineering, Omar Al-Mukhtar University, El-Beida Libya for their financial support.
 Gazali, T. and Moqsud, M. (2017) The Effectiveness of Animal Dungs and Leaf Mold for Bioelectricity Generation Using Microbial Fuel Cell with Soils. Journal of Sustainable Bioenergy Systems, 7, 165-181.
 Femi, T., Ahmed, I., Helen, O. and Satyavolu, J. (2018) Production of Ethanol from Cassava Peelings Using a Developed Percolation Reactor. Journal of Sustainable Bioenergy Systems, 8, 107-115.
 Benali, M., Hamad, T., Hamad, Y. and Belkhair, A. (2019) The Hydrogen Energy Potential of Solid Waste: A Case Study of Misrata City. Advances in Biological Chemistry, 9, 45-53.
 Al Mamun, M.R., Tasnim, A., Bashar, S. and Uddin, M.J. (2018) Potentiality of Biomethane Production from Slaughtered Rumen Digesta for Reduction of Environmental Pollution. AIMS Energy, 6, 658-672.
 Benali, M., Hamad, T., Belkhair, A. and Hamad, Y. (2019) Investigating the Use of Combined Hydrogen, Heat and Power System for Omar Al-Mukhtar University Campus. Advances in Biological Chemistry, 9, 31-44.
 Jardine, J., Sundfeld, E. and Durães, F.M. (2013) Availability of Information about Sustainable Bioenergy Technologies: The Agro-Energy Tree of Knowledge. Journal of Sustainable Bioenergy Systems, 3, 260-264.