AS  Vol.10 No.7 , July 2019
Efficiency of Inoculation with Rhizobium in Peanuts (Arachis hypogaea L.) Grown in Brazilian Cerrado Soil
Abstract: In Brazil, the peanut culture has been expanding mainly in the state of São Paulo, responsible for 80% of the national production. It is an edible culture produces oil of excellent quality, contains proteins rich in amino acids that make up to 36% of weight of the grain. Furthermore, it is associated with nitrogen-fixing bacteria. However, the practice of inoculation is not very common in peanuts, being this species considered susceptible to nodularity with a large number of rhizobia. However, known’s that inoculation with selected strains can increase the efficiency of symbiosis and peanut yield. Thus, the present study was proposed to evaluate the development of peanut inoculated with rhizobia and grown in Cerrado’s Oxisol. The experiment was conducted in a greenhouse, in a completely randomized design, five treatments, one cultivar (IAC 505) and four replicates. The treatments were composed of three strains of rhizobia and two controls, one absolute and the other with nitrogen fertilizer (50 mg⋅N⋅dm3). Each experimental plot has been consisted an 8 dm3 pot with three plants. The inoculation was done, applying 3 mL of bacterial broth (108 mL−1 cells) in the root system of each plant. The SPAD index, number and dry weight of nodules, dry mass of shoots and roots, number and weight of grains were analyzed. The response of Peanuts inoculated was significantly, as all inoculated plants showed similar development to nitrogen-fertilized, emphasizing the potential for supplying nitrogen in peanut development.
Cite this paper: Guimarães, S. , Bonfim-Silva, E. , de Souza, A. and Simeon, B. (2019) Efficiency of Inoculation with Rhizobium in Peanuts (Arachis hypogaea L.) Grown in Brazilian Cerrado Soil. Agricultural Sciences, 10, 948-956. doi: 10.4236/as.2019.107072.

[1]   Bolonhezi, D., Mutton, M.Â. and Martins, A.L.M. (2007) Conservation Tillage to Peanut Crop in Rotation with Green Harvest Sugarcane. Pesquisa Agropecuária Brasileira, 42, 939-947.

[2]   Junior, A.F.C., de Oliveira, L.A. and de Oliviera, A.N. (2009) Indole-Acetic Acid Production by Rhizobia Isolated from Cowpea. Microbiology, 812-817.

[3]   De Medeiros, E.V., Martins, C.M., Lima, J.A.M., Fernandes, Y.T.D., De Oliveira, V.R. and Borges, W.L. (2009) Morphological Diversity of Rhizobia from Cowpea Cultivated in Rio Grande Do Norte State Soils. Acta Scientiarum. Agronomy, 31, 529-535.

[4]   Angelini, J., Ibáñez, F., Taurian, T., Tonelli, M.L., Valetti, L. and Fabra, A. (2011) A Study on the Prevalence of Bacteria that Occupy Nodules within Single Peanut Plants. Current Microbiology, 62, 1752-1759.

[5]   Fabra, A., et al. (2010) Interaction among Arachis hypogaea L. (Peanut) and Beneficial Soil Microorganisms: How Much Is It Known. Critical Reviews in Microbiology, 36, 179-194.

[6]   Embrapa (2013) Brazilian Soil Classification System. 3rd Edition. Embrapa, Brasilia.

[7]   Sousa, E. and Lobato, D.M.G (2002) Correction of Soil and Fertilization Planaltina. Embrapa Cerrados, Brasilia, 185-226.

[8]   Godoy, L.K., Carvalho, I.J., Martins, C.L., Bolonhezi, A.L.M., Freitas, D., Kasai, R.S., Ticelli, F.S., Santos, M., Oliveira, J.F. and Morais, E.J. (2009) IAC 503 and IAC 505: Peanut Cultivars with the Characteristic “High Oleic”. 5th Brazilian Congress on Plant Breeding, Guarapari.

[9]   Hungria, M. and Nogueira (2014) Tecnologia de coinoculação: Rizobium e Azospirillum em soja e feijoeiro.

[10]   Ferreira (2011) Sisvar: A Computer Statistical Analysis System. Ciencia e Agrotecnologia (UFLA), 35, 1039-1042.

[11]   Smeal, D. and Zhang, H. (1994) Chlorophyll Meter Evaluation for Nitrogen Management in Corn. Communications in Soil Science and Plant Analysis, 25, 1495-1503.

[12]   Chapman, S.C. and Barreto, H.J. (1997) Using a Chlorophyll Meter to Estimate Specific Leaf Nitrogen of Tropical Maize during Vegetative Growth. Agronomy Journal, 89, 557-562.

[13]   Santos, C.E.R.S., et al. (2009) Effectiveness of Rhizobia Isolated from Soils of the Northeast Region of Brazil in the Fixation of N2 in Peanuts (Arachis hypogaea L.). Acta Scientiarum. Agronomy, 27, 301-307.

[14]   Vieira, C.L., Freitas, A.D., Silva, A.F., Sampaio, E.V. and Araújo, M.S. (2011) Inoculation of Local Cowpea Varieties with Selected Rhizobia Strains. Revista Brasileira de Engenharia Agrícola e Ambiental, 14, 1170-1175.

[15]   Santos Andrade, J.A., Viana, J.S., Cordeiro Junior, J.J.F., da Silva, A.C., Gonçalves, E.P. and Costa, D.S. (2015) Production of Peanut Intercropped with Forage Palm in Pernambuco State, Brazil. American Journal of Plant Sciences, 6, 818-825.

[16]   Ramos, A.S., Santos, T.M.C., Santana, T.M., et al. (2010) Action of Azospirillum lipoferum on the Development of Corn Plants. Revista Verde, 5, 113-117.

[17]   Reis, V.R.R., Souza, L.R.S., Vieira, G.L.S., Coelho, K.B.S., Carmo Filho, A.D.S., and Silva, M.R.M. (2019) Vegetative Growth of Cowpea with Alternative Inoculant. Revista Verde de Agroecologia e Desenvolvimento Sustentável, 13, 466.

[18]   Veronezi, S.D.F., Costa, F.M., SIlva, M.R. and Mercante, A.T. (2012) Co-Inoculation of Rhizobium and Azospirillum brasilense in Common Bean (Phaseolus vulgaris L.). Cadernos de Agroecologia, 7, 1-5.

[19]   Fernandes, M.F., Fernandes, R.P.M. and Hungria, M. (2003) Selection of Natives Rhizobia to the Cowpea, Pigeonpea and Jackbean Crops in the Coastal Tableland of Sergipe, Brazil. Pesquisa Agropecuária Brasileira, 38, 835-842.

[20]   Ruschel, A.P., Suhet, A.R., Vianni, D. and Almeida, L.R. (1975) Effect of Different Nitrogen Sources and Inoculation on Seed, Protein and Oil Production in Two Soybean Cultivars. Pesquisa Agropecuária Brasileira, 10, 19-23.

[21]   Zilli, J.é., Gianluppi, V., Campo, R.J., Rouws, J.R.C. and Hungriin-Furrow, M. (2010) Inoculation with Bradyrhizobium Alternatively to Seed Inoculation of Soybeana. The Revista Brasileira de Ciência do Solo, 34, 1875-1881.

[22]   Hoffman, L.V., Jacome, R.G. and Suassuna, T.M.F. (2007) Selection of Rhizobia Strains for Nodulation of Peanuts. Revista Brasileira de Oleaginosa e Fibrosa, 11, 107-111.

[23]   Cavalcante, A., Cássio, A., Cavalcante, P., Guilherme, A., Alexandre, M. and Neto, D. (2016) Nitrogen-Fixing Bacteria and Organic Substrates on Growth and Chlorophyll Indices of Peanut. Agropecuária Técnica, 37, 1-8.

[24]   Almeida, A.L.G., Alcantara, M.C.M., Nóbrega, A.L., Leite, S.A. and Silva, F.C. (2010) Productivity of Cowpea cv BR 17 Gurguéia Inoculated with Symbiotic Diazotrophic Bacterial in Piauí. Revista Brasileira de Ciências Agrárias, 5, 364-369.