Soil pollution refers to the presence of chemical or substance out of place and/or present at higher than the normal concentration that has adverse effects on any non-targeted organism , and poses an increasing threat to human health and environmental quality  . Industrialization, wars, mining, and intensification in agriculture have left a legacy of contaminated soils around the world      . The term “heavy metals” refers to the group of metals and metalloids which are naturally occurring in soil , and relatively high atomic mass (>4.5 g/cm3) such as Pb, Cd, Cu, Hg, Sn, and Zn, that can cause toxicity problems     . Their concentrations increased by geological and anthropogenic activates, the metal from anthropogenic tend to be more mobile than those from pedogenic or lithogenic sources  such as the excess use of agrochemicals , sewage sludge and municipal waste disposal   . Soil contamination with heavy metals Sprawls in many regions of the world     . Unlike organic contaminants, heavy metals cannot be degraded   and easy to contaminate the food chain    . Soil can easily be polluted by heavy metal from many sources, such as atmospheric deposition, sewage irrigation, improper stacking of the industrial solid waste, mining activities, the use of pesticides and fertilizers   . Globally, 80 percent of municipal wastewater is discharged into water bodies untreated, and industry is responsible for dumping millions of tonnes of heavy metals, solvents, toxic sludge and other wastes into water bodies each year  . Agriculture, which accounts for 70 percent of water abstractions worldwide, plays a major role in soil pollution, as the use of industrial or municipal wastewater in agriculture is a common practice in many parts of the world. Fifteen years ago, at least 20 million hectares in 50 countries were irrigated with un- or partially treated wastewater   . In fact, wastewater use varies considerably from one region to another, in Hanoi, Vietnam, for instance, up to 80 percent of vegetables produced are irrigated with wastewater  . Irrigation plays a crucial role in agriculture in arid and semi-arid climate zones , it is very important for food security. In zones where annual precipitation was insufficient for rain-fed agriculture, such as the areas with a Mediterranean climate in the Near East, irrigation could make a major economic difference  . In Orontes basin, the accelerated demand for water pushes local farmers to use non-conventional water for irrigation as the sources of fresh water became short. The non-conventional water in last decades becomes more polluted because of climate change, population growth, and increasing of human activities. Wastewater irrigation has opposite aspects; beneficial which providing a reliable source of water supply, when the availability of good quality water becomes scarce , its reliability in arid areas  , adding valuable plant nutrients and organic matter to soil   . Despite these advantages, it has detrimental aspect where it can profoundly affect the soil moisture, aeration regime, surface properties, biological activity, toxic chemicals like heavy metals and pesticides in the water  and many other environmentally important functions of the soil system. The use of treated and non-treated wastewater for agricultural irrigation, which is rich in heavy metal, is common in arid and semiarid regions as a solution to water scarcity    . Although soil often acts as a filter, purifying and immobilizing many of the impurities deposited in it, its capacity is limited. The cumulative effects of atmospheric pollutants, agrochemicals and fertilizers, industrial and domestic solid residues, and toxic and radioactive materials, can negatively affect the soil  . Some Soil properties affected metal availability such as soil pH , moisture content and water holding capacity  . Soil contamination with heavy metals may also cause changes in the composition of soil microbial community, adversely affecting soil characteristics     . On the other hand, the mobility of heavy metals is slow in clayey alkaline soils with a high content of calcium carbonate and their utility is limited due to immobilization  .
Geostatistical methods are often used for prediction mappings of soil properties, because it can calculate unbiased estimates at un-sampled locations  -  . Since pollution in soil presents a continuum in their spatial variations, geostatistical methods are widely used to display this variation and to reduce costs of investigation and to recognize the contamination on sources    . The aims of this study are: 1) to investigate the contents of four heavy metal (Cu, Zn, Cd and Pb) in both river water and soil of pilot area of Orontes basin, 2) to characterize the spatial distributions of this heavy metal, 3) to provide an insight into possible heavy metal contamination by generation of the predicted maps.
2. Study Area
The study area: The Orontes basin is an important agricultural area of irrigated land in Midwest of Syria, with a total area of 23,967 km2, extended from NE of Lebanon through Syria to the South of Turkey. Orontes basin plays an important role in the agriculture economy of Syria, whereas irrigated winter wheat, sugar beet and cotton are growing depending mainly on Orontes river water, which is highly polluted by both domestic sewerage and sludge sewerages  . Orontes river is considered one of the most polluted rivers in the eastern Mediterranean due to high evapotranspiration, a large number of different industries and lack of sewage water treatment plants.
The area of Orontes basin extended around Hama city (northwest to the southwest) was chosen as a pilot area 35.05 - 35.20N and 36.85 - 36.67E. The area is characterized by intensive agricultural and industrial activity, and includes the city of Hama and its environs and residential communities in Ghor Al-Assi, Genan Al-Asi, Sreiheen, Al Daheria, Kazo, Shirraaya, Barza, and Khattab (Figure 1).
In recent decades, agricultural intensification has occurred in parallel with industrial (Metallurgical and Chemical) and urban expansion; these exert a high pressure on the soils and could result in contamination. Agricultural practices used in this area, such as the intensive use of agrochemical products and wastewater, for soil irrigation, result in contamination that requires in-depth investigation. In addition, the concentration of industrial areas near villages and the presence of small industries in the agricultural zones could increase the concentration of pollutants, which should be re-assessed.
The land-use patterns are typical and representative of Mediterranean semiarid climate with 342 mm precipitation, maximized in winter and 19.3˚C annual temperature. The summer is hot dry in which the evapotranspiration exceeds precipitation, with 144 dry days starting from 25 May to 15 of October (Figure 2). According to aridity index, most of the area is dry, which suggests huge demand for water for irrigation.
3. Materials and Methods
3.1. Field Work and Data Collection
In order to provide an insight into possible heavy metal contamination that may be occurring in the study area, the spatial distribution of heavy metal has been mapped for the pilot area using data from river water and soil samples, the data were interpolated using ordinary kriging and prediction mappings of polluted areas were generated.
Water samples have been taken in the irrigation session along the flow of the Asi River from the southeast to the northwest from five monitoring sites (Al Gassalat, Genan Al Asi, Al ArbaaNawheer, Al Daheria, and Arza). Bulk soil samples of 93 observation sites were collected from 30 cm of the plow layer and from 30 - 60 cm of the subsoil; the data from the last depth did not present in this study. The grid net survey of 0.5 km intervals was conducted (60 grid SE, 60 grid NW) (Figure 3).
Location coordinates for all sampled sites were determined using Global Positioning System (GPS) where data are to be used by Geographical Information System (GIS) for data processing. Before analysis, the soil samples were drying in an oven at 50˚C for overnight, grounded finely and sieved through a 63 µm plastic sieve. Analyses of total content of heavy metals (Cd, Pb, Zn, Cu) were performed by using the high quality concentrated (70% w/v) nitric acid, hydrogen peroxide (35%) and hydrochloric acid (38%). the samples were placed in Teflon tubes and digested with HNO3, HF, and HClO4. Then the solutions were diluted with 2% (v/v) HNO3 to a final volume of 50 ml, and then the samples were analyzed using an atomic absorption spectrophotometer. Particle-size distribution of bulk soil was determined by the sieve-hydrometer method  . The organic carbon content in fine earth was determined by dichromate oxidation  . The soil pH was determined in 1:2 soil/water solution.
Figure 1. Location of the study area.
Figure 2. Ombrothermic diagrams of Hama metrological station.
Figure 3. The grid net of water and soil sampling.
3.2. Pollution Assessment
By applying Geo-accumulation Index (Igeo) ,
where Cn is the measured content of an element, and Bn is the background or pristine value of the element. The numerical results are indicative of different pollution classes. The degree of metal pollution is assessed in terms of seven contamination classes based on the increasing numerical value of the index as follows:  .
· I-geo < 0 means unpolluted
· 0 ≤ I-geo < 1 means unpolluted to moderately polluted
· 1 ≤ I-geo < 2 means moderately polluted
· 2 ≤ I-geo < 3 means moderately to strongly polluted
· & 3 ≤ I-geo < 4 means strongly polluted
· & 4 ≤ I-geo < 5 means strongly to very strongly polluted
· & I-geo ≥ 5 means very strongly polluted.
Enrichment Factor (EF) to assess the presence and intensity of anthropogenic contaminant deposition on surface soil ,
EF = (Metal/RE)soil/(Metal/RE)background
Where RE is the value of metal, adopted as Reference Element. The five contamination categories are recognized on the basis of the enrichment factor as follows  :
· EF < 2 is deficiency to minimal enrichment
· EF = 2 - 5 is moderate enrichment
· EF = 5 - 20 is significant enrichment
· EF = 20 - 40 is very high enrichment
· EF > 40 is extremely high enrichment
3.3. Pollution Mapping
Geostatistical analyses were applied through the analysis of semivariograms of the selected individual variables. Experimental semivariograms were obtained from the omnidirectional semivariances, γ(h), as a set of spatial observations, Z(xi), which were calculated as:
where: and are experimental measures of any two points separated by the vector h, and N(h) is the number of experimental pairs separated by h. In practice, a semivariogram simply enumerates the relationship between the degree of similarity between two measurements of some variable Z(xi) separated by distance h, which is termed the lag.
4. Results and Discussion
4.1. Pollutants in the River’s Water
The Physicochemical parameters and heavy metal concentrations of Orontes river water were shown in Table 1.
The water pH is indicating moderately alkaline nature, while the Electrical Conductivity (EC) has referred to increasing problem. There was no irrigation problem related to ammonium nitrogen and phosphate phosphorous.
Table 1. Chemical analysis of Orontes River Water.
According to Geo-accumulation Index (Igeo), the concentration of heavy metal denoted a moderately to strong pollution for Cd and Pb, and very strongly pollution for Cu, which is higher of  recommended threshold. Although the index is for sediment, it gives the indication that accumulation will increase in soils when the soil is irrigated with such irrigation water
4.2. The Physicochemical Properties of the Soil
The pH and organic matter content of the soils are very important factors in controlling mobility and concentration of elements in the soils. The pH values ranged from 7.5 to 8.6 (Figure 4). The results indicate that the soils within the study area are alkaline; (higher pH values) not favor the availability of cations in soil  . That might be explained by the calcareous origin of the parent material as the soil total calcium carbonate content ranging from 23% to up 44%. The Organic matter (OM) content in soil showed high value comparing to soil existing in the area, ranging from less than 1% to 9.8% (Figure 4).
The higher content near sewage stations supports the idea of higher content due to direct irrigation with sewage sludge water, and this indicates that metals are more likely to be bound to organic matter to form metal-chelate complexes, and this would also result in less availability of metals to plants   .
Clay content is high (clay ranges from 12% to more than 40%) and the soil has clay loam to clayey texture. This may be the result of continuous deposition of alluvium on the riverbed in Orontes River (Figure 5).
The absence of clay migration process is a result of continuous soil cultivating and extensive agriculture rotation. In addition, oxidation and terraces of redox-morphic features observed, this is related to irrigation method (flood irrigation) applying by local farmers.
Clayey and high calcium carbonate soil gives the area a high potential for adsorption of heavy metals.
4.3. Soil Heavy Metals Concentration and Distribution
The spatial variations of heavy metal in soils were more significant than that in river water. Geostatistical analysis showed that data did not well-distributed because of the high variation between the studied elements data.
Figure 4. The soil pH and Organic matter (OM) content.
Figure 5. The soil clay content.
The (Cu) concentrations were high in some area (Al Dahreah, near the steel factory and Al Genan) and reach to 127 μg·g−1 (Figure 6), which is higher than allowable limit of heavy metal concentration in soil (Germany 40.0, and the USA 75.0 μg·g−1  ). In addition, a high concentration of (Cu) was significantly associated with organic matter content. However, as (Cu) is considered more readily soluble. The (I geo) was 1.49 refers to moderate pollution, and (EF) was 4.24 refers to moderate enrichment.
The highest concentrations of (Cd) (9.8) μg·g−1 were near Zourblehassenand near Srehen, where Steel and Wheel factories exist (Figure 7) (Germany 1.0, and the USA 1.9 μg·g−1). The (I geo) was 6.64 refers to very strongly pollution, and (EF) was 6.64 refers to significant enrichment. The spatial distribution of Cdwas irregular; it may be that is the result of land use and land management (irrigated, rainfed) and the irrigation method.
The concentration of (Pb) was in general within the limits, except for riverbanks where the values were higher, in some site like Al Dahrieh, Sraheen, and Khattab the values exceeded 95 μg·g−1 (Figure 8) (Germany 40.0, and the USA 15.0 μg·g−1). The (I geo) was 4.22 refers strongly to very strongly pollution, and (EF) was 3.5 refers to moderate enrichment.
The high concentrations of Pbin some points are due to the indiscriminate firing of solid household rubbish (dry batteries) and the presence of paint manufacturing workshops.
Total (Zn) concentration was higher variability and values ranged from 13 to 760 μg·g−1, its environmental risk will be more serious (Figure 9) (Germany 150.0, and USA 140.0 μg·g−1). The (I geo) was 4.7 refers strongly to very strongly pollution, and (EF) was 6.6 refers to significant enrichment. The high concentrations of Zn at Jinan and Arza sites, explained by the existing of sewage treatment plant, while a significant increase in values was found even to the depth of 60 cm in Al Dahreah, near the sewage station. The spatial distributions of Zn concentrations in soil were un-regular; the higher concentration near riverbanks than in the rest soils could be the result of periodical waterlogging and the long period of water stagnation near riverbanks.
Figure 6. Spatial distribution of Cu concentration in the upper soil layer.
Figure 7. Spatial distribution of Cd concentration in the upper soil layer.
Figure 8. Spatial distribution of Pb concentration in the upper soil layer(Pb).
Figure 9. Spatial distribution of Zn concentration in the upper soil layer.
Mapping of four heavy metals (Cd, Cu, Pb, and Zn) in middle Orontes basin gave a comprehensive view and saved time, labor and money consuming, and showed a significant accumulation of studied elements in both river water and surrounding soil. The concentration of four heavy metals was higher in soil samples nearby center area than that in boundary samples except for Zn. The spatial distribution of Cu and Pb concentrations in soil was irregular; while as the Cu was preferentially found in the soil with high organic matter content. These results require constant monitoring and identification of these pollutants accurately so that pollution can be prevented and heavy metals can be disposed of. The correlations for these heavy metals are related to the geographical distributions in the study area, indicating a trend of spatial concentrations increasing with the direction of river flow from southeast to northwest.
The research was funded by the Philip Schwarz Initiative. Many thanks to the Hama Center for Agricultural Scientific Research for conducting laboratory analyzes.
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