The Lubigi Feacal Sludge and Wastewater Treatment Plant in Kampala, Uganda was commissioned in May 2014 and has a capacity to treat 400 m3 of feacal sludge and 5000 m3 of wastewater per day   . Faecal sludge (FS) comes from onsite sanitation technologies (pit latrines, unsewered public ablution blocks, septic tanks, aqua privies, and dry toilets) and has not been transported through a sewer. It is raw or partially digested, a slurry or semisolid, and results from the collection, storage or treatment of combinations of excreta  .
Sewage sludge is waste coming from municipal wastewater treatment plants and it contains at least 80% of water and nutrient-rich organic materials, inorganic compounds, including traces of non-biodegradable heavy metals such as lead (Pb), silver (Ag), cadmium (Cd) and others  . Hence the sludge has a high potential to be used for fertilization of gardens  . However, if the sewage sludge has heavy metals in concentrations higher than the permissible limits, it is not suitable for reuse in gardens and other activities as it is hazardous to the environment  . This is because the soluble heavy metal ions bioaccumulate in organisms through food chain posing harmful health threats to the organisms and the environment   . For instance, leafy and non-leafy vegetables of food plants are good accumulators of heavy metals and the bioaccumulation pattern in non-leafy vegetables increases in the order; leaf > root = stem >tuber. These heavy metals end up in human beings and animals who consume the vegetables. Heavy metals are also known to have strong influence on nutritional values of plants. Plants grown on metal-contaminated soils are nutrient deficient and consumption of such vegetables may lead to nutritional deficiency in the population  .
Lubigi feacal sludge and wastewater treatment receive feacal sludge from toilets and latrines in Kampala city. The plant handles wastewater from Mulago hospital, Makerere University and other different locations around Kampala city with many business activities including education institutions, laboratories, garages, workshops and markets. These activities may be an important source of Pb and Cd contamination through storm runoff especially of electronic wastes from electronic workshops, corrosion of old buildings painted with Pb containing paints, and battery (Lead acid batteries) wastes from battery workshops around town including hospital wastes. However, treated and dried sludge from Lubigi feacal sludge and wastewater treatment plant is used as organic fertilizer by some farmers for enriching agricultural soils with nutrients especially nitrogen and phosphorus  . Much as previous studies of feacal sludge at Lubigi sewage treatment plant showed reduced detectable levels of E. coli, bacteriophages and Ascaris eggs throughout the time of storage when treated with urea and lime  , there is limited monitoring of the levels of heavy metals in sludge from the treatment plant before being used as fertilizer. Therefore, this study assessed the levels of Cd and Pb metals in the treated dried sewage sludge generated by the Lubigi feacal sludge and wastewater treatment plant.
2. Materials and Methods
2.1. Study Area
The study was carried out on the Lubigi feacal sludge and wastewater treatment plant (GPS; N0.33998˚; E32.56032˚). It is located on Lubigi wetland in Kawempe division, Kampala district along the northern bypass Hoima road about 5 km from Kampala city square in Uganda (Figure 1). Lubigi wetland has had most of the natural vegetation cleared due to anthropogenic activities especially in the northern part of the wetland. The socio-economic activities around the treatment plant include agriculture, road construction, settlements and collection of fodder and building materials for sale  . Lubigi waste-water and Sewage Treatment Plant serves Kawempe, Bwaise, Katanga, Makerere, Nsooba and includes hotspots like Mulago Hospital, Public Service and Wandegeya areas and all these have many business activities. Lubigi plant is the second treatment plant in Kampala  . The plant receives and treats wastewater from the piped network and feacal sludge brought by private cesspool emptier trucks  .
Figure 1. Map showing location of Lubigi fecal sludge and wastewater treatment plant. Source: Generated from QGIS 3.2.
2.2. Sample Sites and Sample Collection
With the formal permission from the research management of both National Water and Sewerage Corporation and Lubigi feacal sludge and wastewater treatment plant, sludge samples were randomly collected from different drying beds of the plant in June 2017. Gloved hands were used to pick the sludge samples from the beds and these were put in clean, sterile plastic containers, labeled and then placed in a cool box with ice. After transport, the samples were placed in a refrigerator at 4˚C awaiting further analysis.
Two batches of sludge samples were collected with a time interval of two weeks between the collection of the first batch and second. Two batches of samples were collected to ensure that two different sludge collections at the plant are tested to check for the continuity of the presence of these metals in the sludge generated by the plant. Each batch comprised of 10 samples making a total number of 20 samples.
2.3. Preparation of the Aqua Regia
The aqua regia is a mixture of HCl and HNO3   in a ratio of 3:1 respectively  . A volume of 250 ml of the aqua regia was prepared for each batch of samples following a standard method  . An amount of 187.5 ml of 37% HCl was put in a clean 1000 ml measuring cylinder and 62.5 ml of 65% HNO3 was added to the same measuring cylinder to form a mixture. Freshly prepared aqua regia was used for each batch of samples.
2.4. Preparation of Samples
The samples were prepared according to the method of  and  . The samples were subjected to acid digestion using the aqua regia. A mass of 1.250 g of each sample was ground, air dried, and transferred to a respectively labelled destruction tube (digestion tube). The tubes were placed on a digestion block. Distilled water (50 ml) was then added to each sample in the digestion tube followed by 50 ml of the aqua regia. The digestion block was later switched on and the tubes heated to 100˚C for 1 hour. The heating temperature was increased to 125˚C and heated for 15 minutes followed by 150˚C for 15 minutes and 175˚C for 15 minutes. The heating temperature was further increased to 200˚C and heated until there was no volume of the mixture left, then 5 ml of 65% HNO3 was added. The solution in the tubes was then concentrated to about 5ml. After cooling, 1 ml of 30% H2O2 solution was added and the mixture destructed for 10 minutes. After cooling, 3 ml of 30% H2O2 solution was added and the mixture destructed again for another 10 minutes. Distilled water (50 ml) and 25 ml of 37% HCl were then added and the mixture heated till boiling. The samples were then cooled and filtered into 50 ml volumetric flasks, made up to the mark with 1% HNO3, and transferred into 50 ml falcon tubes. The samples were then allowed to settle for at least 15 hours. The absorbance of the clear supernatant was then measured using an Atomic Absorption Spectrophotometer (AAS). For each run of samples, a blank sample was added and treated the same way as the samples.
2.5. Preparation of Standards
Standard solutions of Pb (10 ml) and Cd (10 ml) were each pipetted into separate 1000 ml volumetric flasks and 20 ml of nitric acid added. Distilled water was then added up to the mark. The solutions were mixed thoroughly and stored for use.
2.6. Metal Analysis by Atomic Absorption Spectrophotometer (AA-6300)
Similar studies have used this method in quantification of trace elements in the sludge, food, soil and water      . Samples were aspirated by the instrument into a burner, desolvated, atomized, and excited to a higher energy electronic state. The use of a flame during analysis required fuel and oxidant, typically in the form of gases. The gas acetylene was the flame used and a hollow cathode lamp of the corresponding element was the resonance. The use of special light sources and careful selection of wavelength allowed the specific quantitative determination of individual elements in the presence of others. Light detectors in the instrument detect light with the analysis information coming from the flame. The information is amplified by a photomultiplier and displayed on the computer. The instrument was designed in such a way that it analyses each sample in triplicate and then computes the average concentration of the metals in each sample. The AA-6300 reported the trace element concentrations in milligrams per kilogram.
2.7. Data Analysis
Microsoft excel version 2011 was used to calculate the descriptive statistics (minimum, maximum, mean, standard deviation) of the concentrations of Pb and Cd in the sludge. The descriptive statistics and the raw values of Pb and Cd in the two sample batches of the sludge are presented in Table 1. Comparison of the mean concentrations of Pb and Cd in batch 1 and batch 2 samples was done using computer software IBM SPSS Version 20 (IBM Corp. Armonk, NY: Released 2011) for independent samples t-test at 5% level of significance and the results also summarized in Table 1.
3. Results and Discussion
Concentration of Pb and Cd in Sludge from Lubigi Sludge Treatment Plant
The results from the batch 1 of samples collected from Lubigi sludge treatment plant showed presence of Pb but Cd was absent (Table 1). The concentration of Pb in the sludge of batch 1 samples ranged from 4.10 to 20.87 mg/kg dry mass with a mean value of 11.80 ± 6.30 mg/kg (Table 1).
Batch 2 samples were collected after 2 weeks interval to monitor if there was any change in the concentration of heavy metals in the sludge. The amount of Pb
Table 1. Concentration (mg/kg dry mass) of Pb and Cd in sludge from Lubigi sludge treatment plant and the EPA maximum permissible limit.
Key: Lbg: Lubigi, SD: Standard Deviation, EPA: Environmental Protection Agency, mg: Milligram, Kg: Kilogram, No: Number, df: Degree of Freedom, Min.: Minimum, Max.: Maximum
in batch 2 samples ranged from 0.93 to 9.18 mg/kg dry mass with a mean concentration of 5.30 ± 2.84 mg/kg. The mean concentration of Pb in Batch 1 samples was significantly (p < 0.05) higher than that of batch 2 samples. Just like batch 1, Cd was also not detected in all the batch 2 samples. The lower levels of Pb in batch 2 than batch 1 is probably caused by the incoming wastewater that is less contaminated.
The mean concentration of Pb in the Lubigi sludge for both batch 1 and batch 2 samples were far below the EPA maximum permissible limit of 840 mg/kg  for any land application as shown in Table 1. The lower mean concentration of Pb than the EPA limit and the undetectable levels of Cd in Lubigi sludge are contrary to the findings from waste stabilization ponds in Dar es salaam, Tanzania where the concentrations of the heavy metals in the sludge were higher than permissible limits  . Hence the sludge from Lubigi pond is suitable for disposal into soil or reuse in gardens.
Furthermore, the concentration of Pb in this study was in low amounts when compared with the study in the Eastern Cape province, South Africa where Pb levels were found to range from 69 to 365 mg/kg d.m for the sewage sludge from sewage treatment plants (STPs)  . In a study in Limpopo Province, South Africa, Pb concentrations in the sludge ranged from 21.3 to 171.85 mg/kg d∙m  but high Pb concentrations in excess of DWAF (Department of Water Affairs and Forestry) guideline values  were found in towns of Polokwane and Louis Trichardt which gives the maximum threshold of Pb at 100 mg/kg d∙m.
It is important to note that though there was very low concentration of Pb in the Lubigi sludge, another study conducted in Kampala city found the Pb content in water samples from Lake Victoria and tap water ranged from 0.32 to 1.25 and 0.09 to 0.19 mg/100 ml, respectively. The Pb content in the vegetables grown alongside highways ranged from 0.53 to 0.95 mg/100 g  and this may come from other sources other than sludge.
It should be noted that the major source of Pb in the environment was probably the use of leaded petrol but since the year 2005, leaded petrol has been phased out from Sub Saharan Africa  . Therefore, the lower Pb concentrations in this study could be due to the phasing out of leaded petrol in Uganda. Lubigi fecal sludge and wastewater treatment plant are located close to high traffic roads from which Pb may enter from other sources for example from other industrial applications and anthropogenic activities.
Cadmium was not detected in all the samples and this means that it is either completely absent in the sludge or present but below detectable levels. Studies on the sewage sludge in the Eastern Cape province, South Africa found Cd levels in the ranges of 1.1 and 1.9 mg/kg  while another study found Cd amounts of 3.10 mg/kg d.m in municipal sewage sludge  . The major sources of Cd were probably the use of paints and plastics and application of rock phosphate fertilizers  and the heavy traffic roads city centres from which Cd may enter storm water drains that are connected to the sewage plant  . The absence or undetectable levels of Cd in this study could be due to the absence of Cd containing pigments/paints/batteries.
Lead concentrations in the sludge generated by the Lubigi fecal sludge and wastewater treatment plant showed significant variation in a short (2 weeks) sampling period possibly attributed to runoff of wastewater from various human activities in the city. These activities include electronic workshops, corrosion of old buildings painted with lead-containing paints, lead-acid batteries and hospital wastes among others. Nevertheless, the concentration of Pb in the sludge is low and far below the EPA maximum permissible concentration for any land application. On the other hand, Cd is either completely absent in the sludge from Lubigi sewage treatment plant or present but far below detectable concentrations. Therefore, sludge from the Lubigi treatment plant is safe for application on agricultural soils as far as Pb and Cd concentrations are concerned.
Special thanks go to the research manager National Water and Sewerage Corporation, Mr. Kanyesigye Christopher, and the manager Lubigi fecal sludge and wastewater treatment plant for granting permission to conduct this study at the plant.
Great thanks go to Mr. Ssazi Job, Mr. Orwiny Martin of Lubigi fecal sludge and wastewater treatment plant for your assistance in sample collection and also unselfishly sharing with me your knowledge that tremendously helped streamline my study.
I am indebted to Ms. Solome Birungi and Mr. Sebulime Stephen of Government Analytical Laboratory and Mr. Eragu Richard of Natural chemotherapeutics laboratory. Without whose guidance I would not have come up with this piece of work. Thank you so much for the unreserved assistance in the laboratory analysis of my research samples.
I extend my gratitude to the Department of Biological Sciences of Kyambogo University for the support and guidance in the accomplishment of this work. May the almighty God reward all of you with abundance.