Back
 AS  Vol.10 No.5 , May 2019
Occurrence of Cassava Mosaic Begomovirus New Species and Ageratum Leaf Curl Cameroon Virus on Pepper (Capsicum annuum L.) in Togo
Abstract: Cassava mosaic disease caused by the whitefly-transmitted begomoviruses (family Geminiviridae) is a major threat to cassava (Manihot esculenta Crantz) production, which can be intercropped with other plants such as pepper (Capsicum annuum L.). The aim of this study is to identify cassava begomoviruses on other crops in cassava intercropping systems. Thus, foliar samples showing typical symptoms of virus diseases in cassava intercropping systems were collected from pepper and submitted to PCR analysis and direct sequencing. Three begomovirus species ACMV, EACMV and ALCCMV were identified and characterized in samples. Isolates of these species shared respectively 90% - 93%, 74% and 80% nucleotide identities with begomoviruses. These findings show that cassava begomoviruses can infect other crops and will help in understanding the epidemiology related to whitefly-transmitted begomoviruses in cassava intercropping systems.
Cite this paper: Dansou-Kodjo, K. , Mivedor, A. , Adjata, K. , Duclercq, J. and Gumedzoe, Y. (2019) Occurrence of Cassava Mosaic Begomovirus New Species and Ageratum Leaf Curl Cameroon Virus on Pepper (Capsicum annuum L.) in Togo. Agricultural Sciences, 10, 671-682. doi: 10.4236/as.2019.105052.
References

[1]   Pita, J.S., Fondong, V.N., Sangare, A., Otim-Nape, G.W., Ogwal, S. and Fauquet, C.M. (2001) Recombination, Pseudo Recombination and Synergism of Geminiviruses Are Determinant Keys to the Epidemic of Severe Cassava Mosaic Disease in Uganda. Journal of General Virology, 82, 655-665.
https://doi.org/10.1099/0022-1317-82-3-655

[2]   Stanley, J., Bisaro, D.M., Briddon, R.W., Brown, J.K., Fauquet, C.M., Harrison, B.D., Rybicki, E.P. and Stenger, D.C. (2005) Geminiviridae. In: Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U. and Ball, L.A., Eds., Virus Taxonomy, 8th Report of the International Committee on Taxonomy of Viruses, Elsevier Academic Press, London, 301-326.

[3]   Mansoor, S., Briddon, R.W., Zafar, Y. and Stanley, J. (2003) Geminivirus Disease Complexes: An Emerging Threat. Trends in Plant Science, 8, 128-134.
https://doi.org/10.1016/S1360-1385(03)00007-4

[4]   Zhou, Y.C., Noussourou, M., Kon, T., Rojas, M.R., Jiang, H., Chen, L.F., Gamby, K., Foster, R. and Gilbertson, R.L. (2008) Evidence of Local Evolution of Tomato Infecting Begomovirus Species in West Africa: Characterization of Tomato Leaf Curl Mali Virus and Tomato Yellow Leaf Crumple Virus from Mali. Archives of Virology, 153, 693-706.
https://doi.org/10.1007/s00705-008-0042-9

[5]   Alabi, O.J., Kumar, P.L. and Naidu, R.A. (2011) Cassava Mosaic Disease: A Curse to Food Security in Sub-Saharan Africa. APSnet Feature.

[6]   Fauquet, C.M., Briddon, R.W., Brown, J.K., Moriones, E., Stanley, J., Zerbini, M. and Zhou, X. (2008) Geminivirus Strain Demarcation and Nomenclature. Archives of Virology, 153, 783-821.
https://doi.org/10.1007/s00705-008-0037-6

[7]   Adjata, K.D., Muller, E., Peterschmitt, M., Aziadekey, M. and Gumedzoe, Y.M.D. (2010) Incidence of Cassava Viral Diseases ANS First Identification of East African Cassava Mosaic Virus and Indian Cassava Mosaic Virus by PCR in Cassava (Manihot Exculenta Crantz) Fields in Togo. American Journal of plant Physiology, 5, 94-101.
https://doi.org/10.3923/ajpp.2008.73.80

[8]   Patil, B.L. and Fauquet, C.M. (2009) Review: Cassava Mosaic Geminiviruses: Actual Knowledge and Perspectives. Molecular Plant Pathology, 10, 685-701.
https://doi.org/10.1111/j.1364-3703.2009.00559.x

[9]   Séka, K., Ouattara, A., Assiri, K.P., Kra, K.D., Hoareau, M., Lefeuvre, P., Atta Diallo, H. and Lett, J.M. (2017) First Report of Pepper Yellow Vein Mali Virus Associated with Pepper Yellow Vein Disease in Cote d’Ivoire. New Disease Reports, 35, 11.
https://doi.org/10.5197/j.2044-0588.2017.035.011

[10]   Tiendrébéogo, F., Traoré, V.S.E., Barro, N., Traoré, A.S., Konaté, G. and Traoré, O. (2008) Characterization of Pepper Yellow Vein Mali Virus in Capsicum sp. in Burkina Faso. Plant Pathology Journal, 7, 155-161.
https://doi.org/10.3923/ppj.2008.155.161

[11]   Dellaporta, S.L., Wood, J. and Hicks, H.B. (1983) A Plant DNA Minipreparation: Version II. Plant Molecular Biology Reporter, 14, 19-21.
https://doi.org/10.1007/BF02712670

[12]   Fondong, V.N., Pita, J.S., Rey, M.E.C., DeKochko, A., Beachy, R.N. and Fauquet, C.M. (2000) Evidence of Synergism between African Cassava Mosaic Virus and a New Double Recombinant Geminivirus Infecting Cassava in Cameroon. Journal of general Virology, 81, 287-297.
https://doi.org/10.1099/0022-1317-81-1-287

[13]   Wyatt, S.D. and Brown, J.K. (1996). Detection of Subgroup III Geminivirus Isolates in Leaf Extracts by Degenerate Primers and Polymerase Chain Reaction. Phytopathology, 86, 1288-1293.
https://doi.org/10.1094/Phyto-86-1288

[14]   Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs. Nucleic Acids Research, 25, 3389-402.
https://doi.org/10.1093/nar/25.17.3389

[15]   Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. and Higgins, D.G. (2007) Clustal W and Clustal X Version 2.0. Bioinformatics, 23, 2947-2948.
https://doi.org/10.1093/bioinformatics/btm404

[16]   Muhire, B.M., Varsani, A. and Martin, D.P. (2014) SDT: A Virus Classification Tool Based on Pairwise Sequence Alignment and Identity Calculation. PLoS ONE, 9, e108277.
https://doi.org/10.1371/journal.pone.0108277

[17]   Padidam, M., Beachy, R.N. and Fauquet, C.M. (1995) Classification and Identification of Geminiviruses Using Sequence Comparisons. Journal of General Virology, 76, 249-263.
https://doi.org/10.1099/0022-1317-76-2-249

[18]   Fortes, I.M., Sánchez-Campos, S., Fiallo-Olivé, E., Díaz-Pendón, J.A., Navas-Castillo, J. and Moriones, E. (2016) A Novel Strain of Tomato Leaf Curl New Delhi Virus Has Spread to the Mediterranean Basin. Viruses, 8, 307.
https://doi.org/10.3390/v8110307

[19]   Navas-Castillo, J., Fiallo-Olive, E. and Sanchez-Campos, S. (2011) Emerging Virus Diseases Transmitted by Whiteflies. Annual Review of Phytopathology, 49, 219-248.
https://doi.org/10.1146/annurev-phyto-072910-095235

[20]   Garrett, K.A., Dendy, S.P., Frank, E.E., Rouse, M.N. and Travers, S.E. (2006) Climate Change Effects on Plant Disease: Genomes to Ecosystems. The Annual Review of Phytopathology, 44, 489-509.
https://doi.org/10.1146/annurev.phyto.44.070505.143420

[21]   Curnutte, L.B., Simmons, A.M. and Abd-Rabou, S. (2014) Climate Change and Bemisia tabaci (Hemiptera: Aleyrodidae): Impacts of Temperature and Carbon Dioxide on Life History. Annals of the Entomological Society of America, 107, 933-943.
https://doi.org/10.1603/AN13143

[22]   Varma, A. and Malathi, V.G. (2003) Emerging Geminivirus Problems: A Serious Threat to Crop Production. Annals of Applied Biology, 142, 145-164.
https://doi.org/10.1111/j.1744-7348.2003.tb00240.x

[23]   Burban, C., Fishpool, L.D.C., Fauquet, C., Fargette, D. and Thouvenel, J.C. (1992) Host Associated Biotypes within West African Populations of the Whitefly Bemisia tabaci (Genn.), (Hom., Aleyrodidae). Journal of Applied Entomology, 113, 416-423.
https://doi.org/10.1111/j.1439-0418.1992.tb00682.x

[24]   Tajebe, L.S., Boni, S.B., Guastella, D., Cavalieri, V., Lund, O.S., Rugumamu, C.P., Rapisarda, C. and Legg, J.P. (2015) Abundance, Diversity and Geographic Distribution of Cassava Mosaic Disease Pandemic-Associated Bemisia tabaci in Tanzania. Journal of Applied Entomology, 139, 627-637.
https://doi.org/10.1111/jen.12197

[25]   Tocko-Marabena, B.K., Silla, S., Simiand, C., Zinga, I., Legg, J., Reynaud, B. and Delatte, H. (2017) Genetic Diversity of Bemisia tabaci Species Colonizing Cassava in Central African Republic Characterized by Analysis of Cytochrome c Oxidase Subunit I. PLoS ONE, 12, e0182749.
https://doi.org/10.1371/journal.pone.0182749

[26]   Romba, R., Gnankine, O., Drabo, S.F., Tiendrebeogo, F., Henri, H., Mouton, L. and Vavre, F. (2017) Abundance of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) and Its Parasitoids on Vegetables and Cassava Plants in Burkina Faso (West Africa). Ecology and Evolution, 8, 6091-6103.
https://doi.org/10.1002/ece3.4078

[27]   Carabalia, A., Bellottia, A.C., Montoya-Lermab, J. and Cuellara, M.E. (2005) Adaptation of Bemisia tabaci Biotype B (Gennadius) to Cassava, Manihot esculenta (Crantz). Crop Protection, 24, 643-649.
https://doi.org/10.1016/j.cropro.2004.11.008

[28]   Alabi, O.J., Ogbe, F.O., Bandyopadhyay, R., Kumar, P.L., Dixon, A.G.O., Hughes, J.d’A. and Naidu, R.A. (2008) Alternate Hosts of African Cassava Mosaic Virus and East African Cassava Mosaic Cameroon Virus in Nigeria. Archives of Virology, 153, 1743-1747.
https://doi.org/10.1007/s00705-008-0169-8

[29]   Adjata, K.D., Woegan, A.Y., Mivedor, A.S., Muller, E., Peterschmitt, M., Sanda, K. and Gumedzoe, Y.M.D. (2014) Wild Plants as Sources of the Permanency of Viruses Infecting Cultivated Plants: Case of Cassava Begomoviruses in Togo. American Journal of Biochemistry and Molecular Biology, 4, 136-142.
https://doi.org/10.3923/ajbmb.2014.136.142

[30]   Ramkat, R., Calari, A., Maghuly, F. and Laimer, M. (2011) Occurrence of African Cassava Mosaic Virus (ACMV) and East African Cassava Mosaic Virus—Uganda (EACMV-UG) in Jatropha curcas. BMC Proceedings, 5, 93.
https://doi.org/10.1186/1753-6561-5-S7-P93

[31]   Leke, W.N., Brown, J.K., Ligthart, M.E., Sattar, M.N., Njualem, D.K. and Kvarnheden, A. (2012) Ageratum Conyzoides: A Host to a Unique Begomovirus Disease Complex in Cameroon. Virus Research, 163, 229-237.
https://doi.org/10.1016/j.virusres.2011.09.039

[32]   Mivedor, A.S., Dansou-Kodjo, K.A., Adjata, K.D., Duclercq, J. and Gumedzoe, Y.M.D. (2017) Molecular Characterization of Genetic Diversity of Tomato (Solanum lycopersicum L.) Begomovirus in Togo. Journal of General and Molecular Virology, 7, 1-9.
https://doi.org/10.5897/JGMV2017.0070

[33]   Maruthi, M.N., Seal, S., Colvin, J., Briddon, R.W. and Bull, S.E. (2004) East African Cassava Mosaic Zanzibar Virus—A Recombinant Begomovirus Species with a Mild Phenotype. Archives of Virology, 149, 2365-2377.
https://doi.org/10.1007/s00705-004-0380-1

 
 
Top