Back
 AiM  Vol.9 No.5 , May 2019
Production of Xylanase and β-Xylosidase Enzymes by Pseudozyma hubeiensis in Solid State Fermentation
Abstract: Xylanase is an important enzyme with potential application in the degradation of xylan component in the lignocellulosic biomass. There are very few reports on the production of cellulase free xylanases especially by yeast strains which have great potential in paper and pulp industry in removing the hemicellulose from the treated or untreated pulp. In this study, P. hubeiensis NCIM 3574 isolated in our laboratory produced significant levels of extracellular cellulase free xylanase (2480 IU/g DSS) in solid state fermentation (SSF) using wheat bran and xylan. It also produced high levels of β-xylosidase (198 IU/g DSS) when grown in SSF using ground nut oil cake and xylan. These highest activities were obtained when fermented Koji was extracted with 1% NaCl supplemented with 0.5% of Triton X-100. These are the highest activities reported so far from yeast strains in the available literature. The crude xylanase preparation of P. hubeiensis produced xylooligosaccharides (XOS) without xylose proving its potential for XOS production with no further requirement of downstream processing. The XOS as prebiotic show beneficial effect on gut microflora such as Lactobacilli and Bifidobacteria which suppress the activity of pathogenic organisms. This xylanase also has a potential application as a bio-bleaching agent in paper and pulp industry.
Cite this paper: Mhetras, N. , Mapare, V. and Gokhale, D. (2019) Production of Xylanase and β-Xylosidase Enzymes by Pseudozyma hubeiensis in Solid State Fermentation. Advances in Microbiology, 9, 467-478. doi: 10.4236/aim.2019.95028.
References

[1]   Gokhale, D.V., Puntambekar, U.S., Vyas, A.K., Patil, S.G. and Deobagkar, D.N. (1984) Hyper Production of β-Glucosidase by an Aspergillus sp. Biotechnology Letters, 6, 719-722.
https://doi.org/10.1007/BF00133063

[2]   Gokhale, D.V., Puntambekar, U.S. and Deobagkar, D.N. (1986) Xylanase and β-Xylosidase Production by an Aspergillus niger NCIM 1207. Biotechnology Letters, 8, 137-138.
https://doi.org/10.1007/BF01048472

[3]   Khisti, U. and Gokhale, D.V. (2013) Purification and Characterization of β-Glucosidases and β-Xylosidase of Aspergillus niger NCIM 1207. Biofuels, 4, 203-217.
https://doi.org/10.4155/bfs.12.92

[4]   Bao, L., Huang, Q., Chang, L., Sun, Q., Zhou, J. and Lu, H. (2012) Cloning and Characterization of Two β-Glucosidase/Xylosidase Enzymes from Yak Rumen Metagenome. Applied Biochemistry and Biotechnology, 166, 72-86.
https://doi.org/10.1007/s12010-011-9405-x

[5]   Alonso, D.M., Hakim, S.K., Zhou, S., Won, W., Hosseinaei, O., et al. (2017) Increasing the Revenue from Lignocellulosic Biomass: Maximizing Feedstock utilization. Science Advances, 3, e1603301.
https://doi.org/10.1126/sciadv.1603301

[6]   Bastawde, K.B., Puntambekar, U.S. and Gokhale, D.V. (1994) Optimization of Cellulase-Free Xylanase Production by a Novel Yeast Strain. Journal of Industrial Microbiology, 13, 220-224.
https://doi.org/10.1007/BF01569752

[7]   Mhetras, N., Liddell, S. and Gokhale, D. (2016) Purification and Characterization of an Extracellular β-Xylosidase by Pseudozyma hubeiensis NCIM 3574 (PhXyl), an Unexplored Yeast. AMB Express, 6, Article ID: 1326.
https://doi.org/10.1186/s13568-016-0243-7

[8]   Gokhale, D.V., Patil, S.G. and Bastawde, K.B. (1998) Potential Application of Yeast Cellulase-Free Xylanase in Agro-Waste Material Treatment to Remove Hemicellulose Fraction. Bioresource Technology, 63, 187-191.
https://doi.org/10.1016/S0960-8524(97)00062-X

[9]   Adsul, M.G., Bastawde, K.B. and Gokhale, D.V. (2009) Biochemical Characterization of Two Xylanases from Pseudozyma hubeiensis Producing Only Xylooligosaccharides. Bioresource Technology, 100, 6488-6495.
https://doi.org/10.1016/j.biortech.2009.07.064

[10]   Rodriguez, C.S. and Sanroman, M.A. (2005) Application of Solid-State Fermentation to Ligninolytic Enzyme Production. Biochemical Engineering Journal, 22, 211-219.
https://doi.org/10.1016/j.bej.2004.09.013

[11]   Adsul, M.G., Terwadkar, A.P., Varma, A.J. and Gokhale, D.V. (2009) Cellulases from Penicillium janthinellum Mutants: Solid State Production and Their Stability in Ionic Liquids. Bioresources, 4, 1670-1681.

[12]   Mahadik, N.D., Puntambekar, U.S., Bastawde, K.B., Khire, J.M. and Gokhale, D.V. (2002) Production of Acidic Lipase by Aspergillus niger in Solid State Fermentation. Process Biochemistry, 38, 715-721.
https://doi.org/10.1016/S0032-9592(02)00194-2

[13]   Wu, H., Cheng, X., Zhu, Y., Zeng, W., Chen, G. and Liang, Z. (2018) Purification and Characterization of Cellulase-Free, Thermostableendo-Xylanase from Streptomyces griseorubens LH-3 and Its Use in Bio-Bleaching on Eucalyptus Craft Pulp. Journal of Bioscience and Bioengineering, 125, 46-51.
https://doi.org/10.1016/j.jbiosc.2017.08.006

[14]   Feniskova, R.V., Tikhomrova, A.S. and Rakhleeva, B.E. (1960) Conditions for Forming Amylase and Proteinase in Surface Culture of Bacillus subtilis. Mikrobiologica, 29, 745-748.

[15]   Ghosh, K. and Mandal, S. (2015) Nutritional Evaluation of Groundnut Oil Cake in Formulated Diets for Rohu, Labeo rohita (Hamilton) Fingerlings after Solid State Fermentation with a Tannase Producing Yeast, Pichia kudriavzevii (GU939629) Isolated from Fish Gut. Aquaculture Reports, 2, 82-90.
https://doi.org/10.1016/j.aqrep.2015.08.006

[16]   Pokorni, D., Cimmerma, A. and Steiner, W. (1997) Aspergillus niger Lipases: Induction, Isolation and Characterization of Two Lipases from MZKI A116 Strain. Journal of Molecular Catalysis B: Enzyme, 2, 215-222.
https://doi.org/10.1016/S1381-1177(96)00031-8

[17]   Al-Asheh, S. and Duvnjak, Z. (1994) The Effect of Surfactants on the Phytase Production and the Reduction of the Phytic Acid in Canola Meal by Aspergillus carbonarius Using a Solid State Fermentation Process. Biotechnology Letters, 16, 183-188.
https://doi.org/10.1007/BF01021668

[18]   Ebune,A., Al-Asheh, S. and Duvnjak, Z. (1995) Effect of Phosphates, Surfactants and Glucose on Phytase Production and Hydrolysis of Phytic Acid in Canola Meal by Aspergillus ficcum during Solid State Fermentation. Bioresource Technology, 54, 241-247.
https://doi.org/10.1016/0960-8524(95)00133-6

 
 
Top