ABSTRACT Phytosterols are a group of steroids alcohols which had been regarded as a functional factor. An unknown compound in phytosterol samples and phytosterol standard samples was detected by HPLC using symmetry C18 column. The quan- tity of the compound was increased with the enrichment of β-sitosterol. After being collected and analyzed by GC-MS and compared with standard diagram from Wiley and Nist standard chart library, it proved to be γ-sitosterol, a 24β epimer of β-sitosterol.
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nullSheng, Y. and Chen, X. (2009) Isolation and identification of an isomer of β-sitosterol by HPLC and GC-MS. Health, 1, 203-206. doi: 10.4236/health.2009.13034.
 V. Piironen, D. G. Lindsay, T. A. Miettinen, etc. (2000) Plant sterols: Biosynthesis, biological function and their importance to human nutrition. J. Sci. of Food and Agric., 80, 939-966.
M. J. Thompson, W. E. Robbins, G. L. Baker, etc. (1963) The nonhomogeneity of soybean-Gamma-Sitosterol. [J]. Steroids., 29(6), 407-418.
I. Nishioka, N. Ikekawa, A. Yagi, ect. (1965) Studies on the plant sterols and triterpenes. [J]. Chem. Pharm. Bull., 11(5), 579-584.
L. J. Goad and T. Akihisa, (1997) Analysis of sterol. Pub-lished by Blackie Academic & Professional, London.