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 JBM  Vol.7 No.4 , April 2019
Cellular Antioxidant Activity and Peroxidase Inhibition of Infusions from Different Aerial Parts of Cassia occidentalis
Abstract: Cassia occidentalis L. is widely used in the world in traditional medicine and especially in some African countries for the treatment of various diseases. The aim of this study was to report the microscopic features, the chromatographic fingerprints and the cellular antioxidant activity and the peroxidase inhibition of infusions from different parts of this plant. Microscopically, leaf can be characterized by cells of the spongy mesophyll and parenchyma numerous cluster crystals of calcium oxalate, paracytic stomata, isolated calcium oxalate cluster crystals, covering and glandular trichomes, scalariform vessels, polyedric starch granules, lignified fibers; flowers by abundant covering and glandular trichomes, spirally thickened vessels and associated parenchyma, abundant pollen grains. Seeds were characterized by pluricellular non-glandular trichomes, epidermis of the testa with underlying oil cells, parenchymatous layers of the testa, thicker-walled cells of the endosperm, pollen grain. Phytochemical analysis revealed the presence of phenolic acids, flavonoids, iridoids, tannins and terpenes. TLC fingerprints of different parts were different and characteristic. They showed the presence of glycosylated flavonoids and phenolic acids as main phytochemicals for flowers, leaves and seedpods. ABTS and DPPH assays showed that infusion extracts have the ability to scavenge free radicals connected with their IC50 values ranging from 21.43 ± 1.25 to 566.24 ± 176.7 mg·mL-1. All extracts showed a weaker capacity to scavenge DPPH radical. Aqueous extracts displayed high cellular antioxidant activity at the concentrations range of 1 - 20 μg·mL-1 using LO-12 on monocytes HL 60. Flower and leave extracts showed more efficient effects on extracellular ROS production. Phenolic compounds could be major contributors to antioxidant activity of infusions of Cassia parts. In MPO (Myeloperoxidase) direct technique, all infusion extracts exhibited a dose-dependent inhibitory effect on MPO activity in the range concentrations of 1 to 20 μg·mL-1 with the leaves and flowers the most active. Obtained results support the potential therapeutic interest of all aerial parts of Cassia and could justify their use in traditional medicine and local nutraceutical resources.
Cite this paper: Ngombe, N. , Ngolo, C. , Kialengila, D. , Wamba, A. , Mungisthi, P. , Tshibangu, P. , Dibungi, P. , Kantola, P. and Kapepula, P. (2019) Cellular Antioxidant Activity and Peroxidase Inhibition of Infusions from Different Aerial Parts of Cassia occidentalis. Journal of Biosciences and Medicines, 7, 83-94. doi: 10.4236/jbm.2019.74009.
References

[1]   Tona, L., Cimanga, R.K., Mesia, K., Musuamba, C.T., Bruyne, T., Apers, S., Vlietinck, A.J., et al. (2004) In Vitro Antiplasmodial Activity of Extracts and Fractions from Seven Medicinal Plants Used in the Democratic Republic of Congo. Journal of Ethnopharmacology, 93, 27-32.
https://doi.org/10.1016/j.jep.2004.02.022

[2]   Yadav, J.P., Arya, V., Yadav, S., Panghal, M., Kumar, S. and Dhankhar, S. (2010) Cassia occidentalis L.: A Review on Its Ethnobotany, Phytochemical and Pharmacological Profile. Fitoterapia, 81, 223-230.
https://doi.org/10.1016/j.fitote.2009.09.008

[3]   Mbuta, K. (2014) Quelques Plantes Utiles du Province de Bas-Congo, République Démocratique du Congo Paul Latham. Mystole Publications, Canterbury.

[4]   Bahati, L.M., Kapepula, P.M., Kabamba, N.N., Moni, B., Kafuti, G.M., Mungitshi, M., Fundu, T.M., et al. (2017) Microscopic Features, Chromatographic Fingerprints and Antioxidant Property of Some Unconventional Green Leafy Vegetables Consumed in Bandundu, DR Congo. Pharmacognosy Communications, 7, 158-163.
https://doi.org/10.5530/pc.2017.4.23

[5]   Wagner, H., Bauer, R., Melchart, D., Xioa, P.-G. and Staudinger, A. (2013) Chromatographic Fingerprint Analysis of Herbal Medicinal: Thin-Layer High Performance Liquid Chromatography of Chinese Drugs. Vol. 3, Springer International Publishing, Berlin.

[6]   Kapepula, P.M., Kabamba Ngombe, N., Tshisekedi Tshibangu, P., Tsumbu, C., Franck, T., Mouithys-Mickalad, A., Frédérich, M., et al. (2017) Comparison of Metabolic Profiles and Bioactivities of the Leaves of Three Edible Congolese Hibiscus Species. Natural Product Research, 31, 2885-2892.
https://doi.org/10.1080/14786419.2017.1305382

[7]   Franck, T., Mouithys-Mickalad, A., Robert, T., Ghitti, G., Deby-Dupont, G., Neven, P. and Serteyn, D. (2013) Differentiation between Stoichiometric and Anticatalytic Antioxidant Properties of Benzoic Acid Analogues: A Structure/Redox Potential Relationship Study. Chemico-Biological Interactions, 206, 194-203.
https://doi.org/10.1016/j.cbi.2013.09.009

[8]   Gurav, S.S. and Gurav, N.S. (2014) Indian Herbal Drug Microscopy. Springer, Berlin.
https://doi.org/10.1007/978-1-4614-9515-4

[9]   Odeja, O., Obi, G., Ogwuche, C.E., Elemike, E.E. and Oderinlo, Y. (2015) Phytochemical Screening, Antioxidant and Antimicrobial Activities of Senna occidentalis (L.) Leaves Extract. Clinical Phytoscience, 1, 6.
https://doi.org/10.1186/s40816-015-0007-y

[10]   Manikandaselvi, S., Vadivel, V. and Brindha, P. (2016) Studies on Physicochemical and Nutritional Properties of Aerial Parts of Cassia occidentalis L. Journal of Food and Drug Analysis, 24, 508-515.
https://doi.org/10.1016/j.jfda.2016.02.003

[11]   Singh, V.V., Jain, J. and Mishra, A.K. (2017) Determination of Antipyretic and Antioxidant Activity of Cassia occidentalis Linn Methanolic Seed Extract. Pharmacognosy Journal, 9, 913-916.
https://doi.org/10.5530/pj.2017.6.143

[12]   Khan, A.A., Rahmani, A.H., Aldebasi, Y.H. and Aly, S.M. (2014) Biochemical and Pathological Studies on Peroxidases: An Updated Review. Global Journal of Health Science, 6, 87-98.
https://doi.org/10.5539/gjhs.v6n5p87

[13]   Shiba, Y., Kinoshita, T., Chuman, H., Taketani, Y., Takeda, E., Kato, Y., Kawai, Y., et al. (2008) Flavonoids as Substrates and Inhibitors of Myeloperoxidase: Molecular Actions of Aglycone and Metabolites. Chemical Research in Toxicology, 21, 1600-1609.
https://doi.org/10.1021/tx8000835

[14]   Gau, J., Furtmller, P.G., Obinger, C., Prvost, M., Van Antwerpen, P., Arnhold, J. and Flemmig, J. (2016) Flavonoids as Promoters of the (Pseudo-)Halogenating Activity of Lactoperoxidase and Myeloperoxidase. Free Radical Biology and Medicine, 97, 307-319.
https://doi.org/10.1016/j.freeradbiomed.2016.06.026

[15]   Ray, R.S. and Katyal, A. (2016) Myeloperoxidase: Bridging the Gap in Neurodegeneration. Neuroscience and Biobehavioral Reviews, 68, 611-620.
https://doi.org/10.1016/j.neubiorev.2016.06.031

[16]   Malle, E., Furtmüller, P.G., Sattler, W. and Obinger, C. (2007) Myeloperoxidase: A Target for New Drug Development? British Journal of Pharmacology, 152, 838-854.
https://doi.org/10.1038/sj.bjp.0707358

[17]   Arauz, J., Ramos-Tovar, E. and Muriel, P. (2016) Redox State and Methods to Evaluate Oxidative Stress in Liver Damage: From Bench to Bedside. Annals of Hepatology, 15, 160-173.

[18]   Kavishe, R.A., Koenderink, J.B. and Alifrangis, M. (2017) Oxidative Stress in Malaria and Artemisinin Combination Therapy: Pros and Cons. The FEBS Journal, 284, 2579-2591.
https://doi.org/10.1111/febs.14097

 
 
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