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 AiM  Vol.9 No.3 , March 2019
Impact of Manure Storage Time and Temperature on Microbial Composition and Stable Fly (Diptera: Muscidae) Development
Abstract: Samples are often frozen for preservation until needed for use. It has been a common practice to store fresh dairy manure in the freezer until needed for fly development studies. However, conflicting data have suggested that freezer temperature and duration of manure may impact fly development studies, and it is likely due to the change in microbial comminutes due to the freezer conditions. In this study manure storage conditions were assessed to ascertain how temperatures impact stable fly, Stomoxys calcitrans L., survival to pupation and determine which bacterial populations impacted fly development using massively-parallel sequencing and 16S metagenomic analysis. Stable fly survival to pupation was greater in manure that was stored warm (27˚C) or frozen (-20˚C or -80˚C) for 24 days as compared to fresh manure samples. Refrigeration (4˚C) of the manure for 24 days did not affect fly development and slightly decreased the pupal weights. Over 80 bacterial families were detected by sequencing allowing for a more thorough assessment of changes in bacterial populations. Only minor shifts were observed in bacterial family composition in the manure when refrigerated or frozen for 24 days, but significant population changes were observed when the manure was incubated for 24 days at 27˚C. Since it is the temperature and incubation time that yielded the greatest pupation rate, it is hypothesized that the manure microbial community impacts the growth and development of stable flies. This study has determined suggested freezer conditions for the best storage of manure samples to maintain bacterial diversity and retain the closest bacterial populations to freshly collected manure. Although untouched, aged (20 days) manure is best to use to assess fly development, it is not always feasible in laboratory experimentations. This study demonstrates the importance of preservation techniques on manure samples, which could also confer to storage of other biological specimens that contain resident microbes.
Cite this paper: Speshock, J. , Brady, J. , Eastman, J. , Roach, T. , Hays, S. and Kattes, D. (2019) Impact of Manure Storage Time and Temperature on Microbial Composition and Stable Fly (Diptera: Muscidae) Development. Advances in Microbiology, 9, 248-265. doi: 10.4236/aim.2019.93018.
References

[1]   Romero, A., Broce, A. and Zurek, L. (2006) Role of Bacteria in the Ovipostion Behavior and Larval Development of Stable Flies. Medical and Veterinary Entomology, 20, 115-121.
https://doi.org/10.1111/j.1365-2915.2006.00602.x

[2]   Taylor, D.B., Moon, R.D. and Mark, D.R. (2012) Economic Impact of Stable Flies (Diptera: Muscidae) on Dairy and Beef Cattle Production. Journal of Medical Entomology, 49, 198-209.
https://doi.org/10.1603/ME10050

[3]   Campbell, J.B., Skoda, S.R., Berkebile, D.R., Boxler, D.J., Thomas, G.D., Adams, D.C. and Davis, R. (2001) Effects of Stable Flies (Diptera: Muscidae) on Weight Gains of Grazing Yearling Cattle. Journal of Economic Entomology, 94, 780-783.
https://doi.org/10.1603/0022-0493-94.3.780

[4]   Talley, J.A., Broce, B. and Zurek, L. (2009) Characterization of Stable Fly (Diptera: Muscidae) Larval Development Habitat at Round Hay Bale Feeding Sites. Journal of Medical Entomology, 46, 1310-1319.
https://doi.org/10.1603/033.046.0609

[5]   Broce, A.B. and Haas, M. (1999) Relation of Cattle Manure Age to Colonization by Stable Fly and House Fly (Diptera: Muscidae). Journal of the Kansas Entomological Society, 72, 60-72.

[6]   Zurek, L. and Albuquerque, T. (2008) Microbial Ecology of Stables Flies: Effect of Bacterial Community of Aging Horse Manure on Stable Fly Fitness. 17th Annual K-State Research Forum, Manhattan, KS, 8 March 2012.

[7]   Lysyk, T.J., Kalischuk-Tymensen, L., Selinger, L.B., Lancaster, R.C., Wever, L. and Cheng, K.J. (1999) Rearing Stable Fly Larvae (Diptera: Muscidae) on an Egg Yolk Medium. Journal of Medical Entomology, 36, 382-388.
https://doi.org/10.1093/jmedent/36.3.382

[8]   Caporaso, J.G., Lauber, C.L., Walters, W.A., et al. (2012) Ultra-High-Throughput Microbial Community Analysis on the Illumina HiSeq and MiSeq Platforms. The ISME Journal, 6, 1621-1624.
https://doi.org/10.1038/ismej.2012.8

[9]   Myers, H.M., Tomberlin, J.K., Lambert, B.D. and Kattes, D. (2008) Development of Black Soldier Fly (Diptera: Stratiomyidae) Larvae Fed Dairy Manure. Environmental Entomology, 37, 11-15.
https://doi.org/10.1093/ee/37.1.11

[10]   Moon, R.D., Hinton, J.L., O’Rourke, S.D. and Schmidt, D.R. (2001) Nutritional Value of Fresh and Composted Poultry Manure for House Fly (Diptera: Muscidae) Larvae. Journal of Economic Entomology, 94, 1308-1317.
https://doi.org/10.1603/0022-0493-94.5.1308

[11]   Geden, C.J. (1999) Host Location by House Fly (Diptera: Muscidae) Parasitoids in Poultry Manure at Different Moisture Levels and Host Densities. Environmental Entomology, 28, 755-760.
https://doi.org/10.1093/ee/28.4.755

[12]   Brady, J.A., Faske, J.B., Castañeda-Gill, J.B., King, J.L. and Mitchell, F.L. (2011) High-Throughput DNA Isolation Method for Detection of Xylella fastidiosa in Plant and Insect Samples. Journal of Microbiological Methods, 86, 310-312.
https://doi.org/10.1016/j.mimet.2011.06.007

[13]   Herlemann, D.P.R., Labrenz, M., Jurgens, K., Bertilsson, S., Waniek, J.J. and Andersson, A.F. (2011) Transitions in Bacterial Communities along the 2000 km Salinity Gradient of the Baltic Sea. The ISME Journal, 5, 1571-1579.
https://doi.org/10.1038/ismej.2011.41

[14]   Klindworth, A., Pruesse, E., Schweer, T., Peplies, J., Quast, C., Horn, M. and Glöckner, F.O. (2012) Evaluation of General 16S Ribosomal RNA Gene PCR Primers for Classical and Next-Generation Sequencing-Based Diversity Studies. Nucleic Acids Research, 41, e1.
https://doi.org/10.1093/nar/gks808

[15]   Illumina (2013) 16S Metagenomic Sequencing Library Preparation.

[16]   Caporaso, J.G., Kuczynski, J., Stombaugh, J., et al. (2010) QIIME Allows Analysis of High-Throughput Community Sequencing Data. Nature Methods, 7, 335-336.
https://doi.org/10.1038/nmeth.f.303

[17]   Edgar, R.C. (2010) Search and Clustering Orders of Magnitude Faster than BLAST. Bioinformatics, 26, 2460-2461.
https://doi.org/10.1093/bioinformatics/btq461

[18]   Hannon. FASTX Toolkit.
http://hannonlab.cshl.edu/fastx_toolkit/

[19]   DeSantis, T.Z., Hugenholtz, P., Larsen, N., et al. (2006) Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB. Applied and Environmental Microbiology, 72, 5069-5072.
https://doi.org/10.1128/AEM.03006-05

[20]   Lozupone, C. and Knight, R. (2005) UniFrac: A New Phylogenetic Method for Comparing Microbial Communities. Applied and Environmental Microbiology, 71, 8228-8235.
https://doi.org/10.1128/AEM.71.12.8228-8235.2005

[21]   Anderson, M.J. (2001) A New Method for Non-Parametric Multivariate Analysis of Variance. Austral Ecology, 26, 32-46.

[22]   Anderson, M.J., Crist, T.O., Chase, J.M., et al. (2011) Navigating the Multiple Meanings of Beta Diversity: A Roadmap for the Practicing Ecologist. Ecology Letters, 14, 19-28.
https://doi.org/10.1111/j.1461-0248.2010.01552.x

[23]   Schmidtmann, E.T. and Martin, P.A.W. (1992) Relationship between Selected Bacteria and the Growth of Immature House Flies, Musca domestica, in an Axenic Test System. Journal of Medical Entomology, 29, 232-235.
https://doi.org/10.1093/jmedent/29.2.232

[24]   Zurek, L., Schal, C. and Watson, D.W. (2000) Diversity and Contribution of the Intestinal Bacterial Community to the Development of Musca domestica (Diptera: Muscidae) Larvae. Journal of Medical Entomology, 37, 924-928.
https://doi.org/10.1603/0022-2585-37.6.924

[25]   Wade, W. (2002) Unculturable Bacteria—The Uncharacterized Organisms That Cause Oral Infections. Journal of the Royal Society of Medicine, 95, 81-83.

[26]   Rettedal, E.A., Gumpert, H. and Sommer, M.O.A. (2014) Cultivation-Based Multiplex Phenotyping of Human Gut Microbiota Allows Targeted Recovery of Previously Uncultured Bacteria. Nature Communications, 5, Article No. 4714.
https://doi.org/10.1038/ncomms5714

[27]   Jami, E. and Mizrahi, I. (2012) Composition and Similarity of Bovine Rumen Microbiota across Individual Animals. PLoS ONE, 7, e33306.
https://doi.org/10.1371/journal.pone.0033306

[28]   Wust, P.K., Horn, M.A. and Drake, H.L. (2011) Clostridiaceae and Enterobacteriaceae as Active Fermentaers in Earthworm Gut Content. The ISME Journal, 5, 92-106.
https://doi.org/10.1038/ismej.2010.99

[29]   Tian, Z., Cabrol, L., Ruiz-Filippi, G. and Pullammanappallil, P. (2014) Microbial Ecology in Anaerobic Digestion at Agitated and Non-Agitated Conditions. PLoS ONE, 9, e109769.
https://doi.org/10.1371/journal.pone.0109769

[30]   Ladnolt, P.J., Cha, D.H. and Zack, R.S. (2015) Synergistic Trap Response of the False Stable Fly and Little House Fly (Diptera: Muscidae) to Acetic Acid and Ethanol, Two Principle Sugar Fermentation Volatiles. Environmental Entomology, 44, 1441-1448.
https://doi.org/10.1093/ee/nvv119

[31]   Meehan, C.J. and Beiko, R.G. (2014) A Phylogenomic View of Ecological Specialization in the Lachnospiraceae, a Family of Digestive Tract-Associated Bacteria. Genome Biology and Evolution, 6, 703-713.
https://doi.org/10.1093/gbe/evu050

[32]   Qi, M., Nelson, K.E., Daugherty, S.C., Nelson, W.C., Hance, I.R., Morrison, M. and Forsberg, C.W. (2005) Novel Molecular Features of the Fibrolytic Intestinal Bacterium Fibrobacter intestinalis Not Shared with Fibrobacter succinogenes as Determined by Suppressive Subtractive Hybridization. Journal of Bacteriology, 187, 3739-3751.
https://doi.org/10.1128/JB.187.11.3739-3751.2005

[33]   Zackular, J.P., Baxter, N.T., Iverson, K.D., Sadler, W.D., Petrosino, J.F., Chen, G.Y. and Schloss, P.D. (2013) The Gut Microbiome Modulates Colon Tumorigenesis. mBio, 4, e00692-13.
https://doi.org/10.1128/mBio.00692-13

[34]   Kim, M., Morrison, M. and Yu, Z. (2011) Status of the Phy-logenetic Diversity Census of Ruminal Microbiomes. FEMS Microbiology Ecology, 76, 49-63.
https://doi.org/10.1111/j.1574-6941.2010.01029.x

[35]   Vandamme, P. and De Ley, J. (1991) Proposal for a New Family, Campylobacteraceae. International Journal of Systematic and Evolutionary Microbiology, 41, 451-455.
https://doi.org/10.1099/00207713-41-3-451

[36]   Khan, S.T., Horiba, Y., Yamamoto, M. and Hiraishi, A. (2002) Members of the Family Comamonadaceae as Primary Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)- Degrading Denitrifiers in Activated Sludge as Revealed by a Polyphasic Approach. Journal of Applied & Environmental Microbiology, 68, 3206-3214.
https://doi.org/10.1128/AEM.68.7.3206-3214.2002

[37]   Angelos, J.A. and Ball, J.M. (2007) Differentiation of Moraxella bovoculi sp. nov. from Other Coccoid Moraxellae by the Use of Polymerase Chain Reaction and Restriction Endonuclease Analysis of Amplified DNA. Journal of Veterinary Diagnostic Investigation, 19, 532-534.
https://doi.org/10.1177/104063870701900511

 
 
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