JBM  Vol.7 No.3 , March 2019
Kanggan Granule Ameliorates Dexamethasone-Induced Immunosuppression in Mice
Abstract: Objective: This study was to investigate the effect of the Chinese herbal compound Kanggan granule (KG) on immune function in a mouse model of immunosuppression and its possible mechanism of action. Method: ICR mice were randomly divided into a normal control group (untreated non-immunosuppressed, Control), untreated immunosuppressed group (Model), positive control group (immunosuppressed and treated with 1.6 g/kg astragalus granule [AG]), high-dose KG group (immunosuppressed and treated with 24 g/kg, KG-24), and low-dose KG group (immunosuppressed and treated with 6 g/kg, KG-6). Each group received intragastric administration once daily for 7 days. Immunosuppression was induced by an intraperitoneal injection of dexamethasone (25 mg/kg) once daily beginning on day 1 for 3 days. To illuminate the mechanism of immunomodulatory, we studied the effects of KG on nonspecific immunity, humoral immunity and cellular immunity in mice respectively. Results: KG improved organ weights and improved the phagocytic ability of mononuclear macrophages in immunosuppressed mice (p < 0.01 or p < 0.05). The proliferation of spleen lymphocytes and number of peripheral blood leukocytes were enhanced after KG treatment in immunosuppressed mice (p < 0.05). KG increased the CD4+/CD8+ ratio in immunosuppressed mice (p < 0.01) and increased serum IL-2 and IgG levels (p < 0.01). Conclusions: KG can improve immune function in immunosuppressed mice. Nonspecific immunity, humoral immunity, and cellular immunity were all enhanced.
Cite this paper: Gan, Y. , Gu, M. , Liu, D. , Zhou, H. , Zeng, C. , Yang, T. , Li, H. , Geng, F. and Du, J. (2019) Kanggan Granule Ameliorates Dexamethasone-Induced Immunosuppression in Mice. Journal of Biosciences and Medicines, 7, 80-91. doi: 10.4236/jbm.2019.73008.

[1]   Rios, J.L. (2010) Effects of Triterpenes on the Immune System. Journal of Ethnopharmacology, 128, 1-14.

[2]   Benton, T., Lynch, K., Dube, B., Gettes, D.R., Tustin, N.B., Ping Lai, J., Metzger, D.S., Blume, J., Douglas, S.D. and Evans, D.L. (2010) Selective Serotonin Reuptake Inhibitor Suppression of HIV Infectivity and Replication. Psychosomatic Medicine, 72, 925-932.

[3]   Jellema, R., Passos, V., Zwanenburg, A., et al. (2013) Cerebral Inflammation and Mobilization of the Peripheral Immune System Following Global Hypoxia-Ischemia in Preterm Sheep. Journal of Neuroinflammation, 10, 807.

[4]   Liu, Y.N. and Zeng, G. (2012) Cancer and Innate Immune System Interactions: Translational Potentials for Cancer Immunotherapy. Journal of immunotherapy, 35, 299-308.

[5]   Chao, J., Dai, Y., Verpoorte, R., et al. (2017) Major Achievements of Evidence-Based Traditional Chinese Medicine in Treating Major Diseases. Biochemical pharmacology, 139, 94-104.

[6]   Tsai, Y.T., Lai, J.N., Lo, P.C., Chen, C.N. and Lin, J.G. (2017) Prescription of Chinese Herbal Products Is Associated with a Decreased Risk of Invasive Breast Cancer. Medicine, 96, e7918.

[7]   Hsu, H.F., Hsiao, P.C., Kuo, T.C., et al. (2016) Antioxidant and Anti-Inflammatory Activities of Lonicera japonica Thunb. var. sempervillosa Hayata Flower Bud Extracts Prepared by Water, Ethanol and Supercritical Fluid Extraction Techniques. Industrial Crops and Products, 89, 543-549.

[8]   Ou, C.B., Zhang, Q., Wu, G.J., Shi, N.N. and He, C. (2015) Dryocrassin ABBA, a Novel Active Substance for Use against Amantadine-Resistant H5N1 Avian Influenza Virus. Frontiers in microbiology, 6, 592.

[9]   Lee, S., Kwon, Y., Son, K., Kim, H. and Heo, M. (2005) Antioxidative Constituents from Paeonia lactiflora. Archives of Pharmacal Research, 28, 775-783.

[10]   Li, R.D., Guo, W.Y., Fu, Z.R., Ding, G.H., Zou, Y. and Wang, Z.X. (2011) Hepatoprotective Action of Radix Paeoniae Rubra Aqueous Extract against CCl4-Induced Hepatic Damage. Molecules, 16, 8684-8694.

[11]   Yang, X.R. (2012) Clinical Observation on Treatment of 40 Cases of Upper Respiratory Tract Infection in Children. Guide of China Medicine, 10, 283-284.

[12]   Ma, Z.P. and Li, G.G. (2011) The Efficacy of Kanggan Granule in Treating 36 Children with Hand-Foot-and-Mouth Disease. Chinese Journal of Experimental Traditonal Medical Formulae, 17, 269-270.

[13]   Li, Y.H., Zheng, B., Tian, H.J., et al. (2017) Yupingfeng Powder Relieves the Immune Suppression Induced by Dexamethasone in Mice. Journal of Ethnopharmacology, 200, 117-123.

[14]   Cheng, X.B., Wang, C.L., Su, Y.Q., et al. (2018) Enhanced Opsonization-Independent phagocytosis and Highresponse Ability to Opsonized Antigen-Antibody Complexes: A New Role of Kupffer Cells in the Accelerated Blood Clearancephenomenon upon Repeated Injection of PEGylated Emulsions. Molecular Pharmaceutics, 15, 3755-3766.

[15]   Xu, S.Y. (2002) Experimental Methodology of Pharmacology. People’s Medical Publishing House, Beijing.

[16]   Xu, P.F., Sun, J.H., Gao, F.Y. and Liu, G.L. (2007) Experimental Study of Astragalus Granule on Immune Function of Mice. Chinese Journal of Information on Traditional Chinese Medicine, 14, 27-28.

[17]   Johansen, P., Weiss, A., Bunter, A., Waeckerle-Men, Y., Fettelschoss, A., Odermatt, B. and Kundig, T.M. (2011) Clemastine Causes Immune Suppression through Inhibition of Extracellular Signal-Regulated Kinase-Dependent Proinflammatory Cytokines. The Journal of Allergy and Clinical Immunology, 128, 1286-1294.

[18]   Sierra-Honigmann, M.R. and Murphy, P.A. (1992) T Cell Receptor-Independent Immunosuppression Induced by Dexamethasone in Murine T Helper Cells. The Journal of Clinical Investigation, 89, 556-560.

[19]   de Almeida, D.A., Rosa, S.I., da Cruz, T.C., et al. (2017) Mandevilla Longiflora (Desf.) Pichon Improves Airway Inflammation in a Murine Model of Allergic Asthma. Journal of Ethnopharmacology, 200, 51-59.

[20]   Maes, M., Lambrechts, J., Bosmans, E., et al. (1992) Evidence for a Systemic Immune Activation during Depression: Results of Leukocyte Enumeration by Flow Cytometry in Conjunction with Monoclonal Antibody Staining. Psychological Medicine, 22, 45-53.

[21]   Liu, J., Wang, L., Feng, Z., Geng, D., Sun, Y. and Yuan, G. (2017) Dynamic Changes of Laboratory Parameters and Peripheral Blood Lymphocyte Subsets in Severe Fever with Thrombocytopenia Syndrome Patients. International Journal of Infectious Diseases, 58, 45-51.

[22]   Zhu, Z.Y., Meng, M., Sun, H., Li, Y., Ren, Y.Y. and Zhang, Y. (2016) Immunostimulatory Activity of Glycopeptides from Paecilomyces sinensis under Normal and Cyclophosphamide Induced Immunosuppressive Conditions in Mice Models. Food & Function, 7, 3566-3576.

[23]   Jarosz, L., Kwiecien, M., Marek, A., Gradzki, Z., Winiarska-Mieczan, A., Kalinowski, M. and Laskowska, E. (2016) Effects of Feed Supplementation with Glycine Chelate and Iron Sulfate on Selected Parameters of Cell-Mediated Immune Response in Broiler Chickens. Research in Veterinary Science, 107, 68-74.

[24]   Matos, L., Munoz, M.D.C., Molina, J.M., et al. (2017) Humoral Immune Responses of Experimentally Eimeria Ninakholyakimovae-Infected Goat Kids. Comparative Immunology, Microbiology and Infectious Diseases, 51, 60-65.