Data from the US Department of Agriculture show that the world consumes approximately 9 million tons of coffee per year. Coffee is an important product for the Brazilian and World economies. Brazil is the largest producer and exporter of coffee in the world and has two economically important species: arabica coffee and canephora coffee (conilon), with arabica coffee accounting for 81% and conilon for 19% of the area under cultivation in the country  .
In order for the coffee to reach the final consumers’ table, a series of processes take place, from harvesting, processing, drying and storage, to industrialization. However, the crucial point in the last decades has been in the wet processing, with the introduction of induced fermentation  and those with spontaneous fermentation studies  .
When done correctly, wet processing with induced fermentation ensures that the intrinsic qualities of the coffee beans are preserved for a long time, producing a homogeneous coffee with few defective grains. Thus, coffee produced by this method is generally considered to be of better quality and commands the highest prices in the global market  .
These processes are commonly complex; each coffee grower has its variations in post-harvest methodologies. Often, no agreement and exact standardization were observed for wet processing  . The shortcomings in controlling coffee processes on site, particularly in wet processing (fermentation), lead to defects and lack of consistency in product quality, causing losses to producers  .
However, there is a great advance in the technology used in coffee plantations and new methods have been used to improve the quality of the grain, mainly in postharvest  . After this stage, coffee needs to be evaluated, so that quality is determined. At this time, the procedure of cup tasting, through tasters, using personal opinion and tasting experience accumulated over the years   . The tasters are judges who perform the sensory analysis and are in charge of evaluating the coffee quality, consequently influencing the evaluation according to their perceptions. Often, it is up to these professionals to determine the final quality, thus interfering with the price of the product  .
Based on the brief outline described above, the question is: What is the potential for acceptance of fermented Arabica coffee in the perception of North American tasters?
This study sought to understand and describe the potential for acceptance of spontaneous and induced fermented Arabica coffee from the perspective of graders from the United States of America. The study was conducted in Austin, Texas, in 2017, with a 6-judge panel that evaluated different methods employed in wet processing.
2. Material and Methods
2.1. Sample Preparation: Wet Processing
The producers, respecting the experimental design in previously demarcated areas, harvested coffee beans of the Catuaí Vermelho variety, from June to September of 2016. The raw materials used in the formulation of the worts were coffee pulp, coffee husk from peeling, water and yeast (Saccharomyces cerevisiae). Fifteen kilograms of coffee were harvested per experimental plot in both experiments, presenting 85% of ripe fruits. After harvest, the fruits were processed according to established procedures.
The raw materials used in the formulation of the musts were composed of: coffee pulp, bark from peeling, water and yeast (Saccharomyces cerevisiae). Twenty kilograms of coffee were harvested per experimental plot in all experiments, and after harvest the fruits were processed according to established procedures. 6 points were selected to develop this study in different places in Espírito Santo Coffee Single Origin.
The five processes had the following methodologies:
Process 1: fermentation must with water (W―Washed), 15 kg of peeled cherry coffee (pulp), 10 kg of bark and 10 liters of water.
Process 2: fermentation must with yeast starter culture (Saccharomyces cerevisiae-YF―Yeast fermentation) in a 1% (part of volume) of must, 15 kg of coffee pulped (pulp) 10 kg of bark, 10 liters of water.
Process 3: dry fermentation must (FWWY―Fully washed without yeast), 15 kg of peeled cherry coffee (pulp) and 10 kg of peel, without additional process water.
Process 4: dry fermentation must with yeast (FWY―Fully washed with yeast), fermentation must with yeast starter culture (Saccharomyces cerevisiae), in the proportion of 1% (p/v) of the must, 15 kg of peeled cherry coffee (pulp), 5 kg of bark, without added water.
Process 5: semi-dry (SD―Semi-dry) process, fermentation with 15 kg peeled cherry coffee (pulp), without water. Drying in African Bed System (spontaneous fermentation).
In the four proposed methods, a wort was prepared with yeast culture (Saccharomyces cerevisiae) and coffee husk. For the experiments, worts 1 and 2 received addition of water to the processing at the temperature of 38˚C  , and remained immersed in fermentation tanks in the processing laboratory of the Federal Institute of Espírito Santo (IFES) for 36 hours. Wort 3 received only coffee husk, from the wet processing, remaining in fermentation for 36 hours. Wort 4 only received husk and yeast (Saccharomyces cerevisiae) without addition of water. After the fermentation period, worts 1, 2, 3 and 4 were washed and taken to dry in a suspended and covered yard  .
2.2. Sensory Analysis
The experiments were conducted in the Laboratory of Sensory Analysis of Casa Brasil Specialty Coffee with North American judges from May to July of 2017.
Roasting was conducted using the Laboratto TGP-2 roaster with the Agtron-SCAA disk set. The roasting point of these samples was set based on grain color between the colors determined by disks #65 and #55 for specialty coffees  .
Roasting was executed within 24 hours of cupping and it was allowed to rest for 8 hours before grinding. All samples were roasted from 9 to 10 minutes. After roasting and cooling, the samples remained stored in sealed containers, according to the methodology of sensory analysis established by the SCAA.
The coffee samples were ground with Mahlkoenig EK-40 electric grinder, in the medium to coarse particle size range. Five cups of each batch of coffee were prepared for cupping. The optimal ratio of 8.25 g of coffee per 150 ml of water was adopted, as this conforms to the mid-point of the balance chart, optimal to achieve the Golden Cup  . The infusion was performed after the water reached 92˚C - 95˚C. The Q-Graders began the evaluation when the temperature of the cups reached 55˚C, respecting the time of 4 minutes from the infusion to the evaluation.
Sample Evaluation Method
A centesimal numerical scale expresses the quality of a given batch of coffee, when evaluated through the SCAA method. It is expected that coffees that get high scores should, evidently, be better than coffees that receive lower scores, thus demonstrating the consistency of the method of descriptive sensory analysis.
The tasting form provides the possibility of evaluating eleven (11) important attributes for coffee: Fragrance/Aroma, Uniformity, Absence of Defects (Clean Cup), Sweetness, Flavor, Acidity, Body, Aftertaste, Balance, Defects and Overall evaluation. Highly positive results arise from the perception of a balanced group formed by the evaluated attributes.
A panel of six (6) cupping tasters carried out the sensory analysis of the coffees, aiming at reducing the subjectivity in the evaluation process   .
2.3. Experimental Design
The experiments were conducted in a randomized block design with five replicates, where five treatments were evaluated. For the statistical analyzes, joint analyzes of the experiments were carried out, in which the means were compared by the Tukey test, the regression models tested using the F test and the estimators by t test. The sensory data were analyzed by the Complete Linkage Hierarchical Clustering method. The goal is to maximize the homogeneity of objects within groups, while maximizing the heterogeneity between the groups  . Statistical analyzes were performed using the software IBMSPSSStatistics Base 22.0.
3. Results and Discussion
1Cupping taster: In the coffee production chain, it is common for the evaluators, or tasters, to carry out the sensory analysis of the samples that are commercialized, from the sale of the raw material, to the final consumer. The cupping taster practice describes the method of sensory analysis that is used in evaluating coffee quality, which is usually employed by tasters or Q-graders with extensive experience.
The sensorial results expressed in Table 1 indicate the perception of North American cupping tasters1. During the sensorial tests, it was possible to observe the
Table 1. Global score means evaluated in five treatments and at six altitudes.
Means followed by the same letter vertically do not differ from each other by the Tukey test at 5% probability.
potential for the wet-processed fermented coffee with the use of yeasts. No significant functional relationships were observed between global score and altitude, at 5% probability.
Based on the data of Table 1, for the altitude of 907 meters, the five proposed treatments did not present statistical differences by the Tukey test at 5% of significance. In the second altitude range, at 969 meters, treatment 4―dry fermentation with yeasts (F.W.Y), showed statistical difference from the other treatments, at 5% of significance by the Tukey test. Thereafter, treatment 2 did not differ from treatment 3 and 5, but differed from treatment 1 and 4, at 5% significance. Finally, treatment 1 showed the worst sensory results in terms of absolute numbers.
In the third experimental range, at 1005 meters altitude, the treatments did not present statistical differences among themselves at 5% of significance. In the 1021-meter range, treatment 4, dry fermentation with yeasts (F.W.Y) differed from the others at 5% significance and consequently had the best sensory results.
In the experimental range of 1052 meters of altitude, treatment 4 did not differ from treatment 2, at 5% of significance, but it differed from the others. It is observable that both treatments that used yeasts had higher scores than the other methods used in the wet processing.
Finally, in the last experimental range, treatment 4 did not differ from treatments, 2, 3 and 5, but differed from treatment 1, at 5% significance. Once again, treatment 4 presented the best sensory results relative to the overall scores.
In the overall mean of the experiments, treatment 4 differed from the other treatments, at 5% significance. Among treatments 2, 3 and 5 there are no statistical differences; however, treatment 2 differs from treatment 1, at 5% significance.
It is possible to infer from the sensory results that all coffees submitted to spontaneous or induced fermentation appear in groups of Specialty coffees, as directed by the Specialty Coffee Association of America, SCAA, that is, they are above the cutoff of 80 points. We observed that treatment 4 (dry fermentation with yeast culture) stands out from the other treatments. It was possible to understand that in the sensory perception, the results found are in agreement with the results of  and the according with   these differences in sensory attributes can probably be attributed to the fermentation process through wet processing.
However, the effects of fermentation during wet processing on the aroma profile of coffee are not completely elucidated and are often neglected, as the literature has argued that the main function of fermentation is the removal of mucilage  . The commercially available starter cultures for fermentation are not designed directly to ferment coffee beans, mainly due to the physical and chemical peculiarities of this process  , reinforcing the need for further studies on the microbiota and the exact conditions of use.
In a complementary way, a multivariate analysis was performed using dendrograms to observe clustering in area (altitude/processing). Figure 1 shows the graphical distribution of the studied points. The dendrogram of Figure 1 suggests the existence of three homogeneous clusters: cluster A, formed by points (altitude/treatment) 9, 19, 24, 27 and 29; cluster B, formed by point 6 and cluster C, formed by the other points.
Observing the data dispersion in Figure 1, cluster A grouped the best sensory results, being constituted by: point 9―altitude 2―fully washed with yeast method; followed by point 19―altitude 4 and FWY method; point 24―altitude 5 and FWY method; point 27, altitude 6 and yeast fermentation method; and point 29, altitude 6 and FWY method.
The second cluster, composed of point 6, isolated the method of spontaneous fermentation with water from all other process ses, at 969 meters, indicating that the use of starter cultures was favorable for the other study areas. The same qualitative relationship was observed for the spontaneous fermentation method in warmer and lower altitude zones. Lastly, all other points compose cluster C. Wecould only observe distancing in point 10, relative to altitude 2, method 5
Figure 1. Distribution of clusters with 24 points, 6 altitudes and 5 processes.
(semi-dry). In this experimental group, the worst results are clustered, therefore, it is necessary to relativize, that in this case, the observed low scores cannot reflect directly on the methods, but rather on the raw material of this area  .
For the construction of the dendrograms, the variables that contributed the most to the clustering were fragrance (23.4%), body (18.6%), aftertaste (10.6%), acidity (7.13%), balance (5.76%), flavor (3.68%), and overall score (29.9%). Based on this indication, it is possible to infer about the cupping tasters’ choice profile, which draws attention to the strong contribution of the variables overall, fragrance and body as decisive factors of choice. The consumer preferences for a particular type of coffee, or even for the method of preparation, may be influenced by several factors, such as culture, lifestyle, social and work environment, daily habits and financial aspects, besides, of course, the flavor and hence, the importance of calibration and international certification. In the case of the cupping tasters used in this study, it was observed that most of the coffees that are evaluated in Casa Brasil Coffees in relation to preference belong to the group of naturals2, that is, sensorial profile that much resembles the fermented coffee using yeasts  .
Thus, two relevant observations can be considered, the first on the possibility of modification of the metabolic routes and consequently change of sensory profile   . The according to  , one of the main problems in using experts in sensory evaluation is that the expert qualification (cupping tasters) is not well defined. In accordance with ISO 856-2 (1994), experience is not only the main criterion of a specialist, since it must be trained and have high sensory sensitivity.
The results demonstrated potential for wet-processed fermented coffee from the perspective of North American cupping tasters. In sequence, we suggest the comparison of the evaluated coffees by Brazilian cupping tasters, to measure the possible associations of preference according to the different types of prepared coffees.
Dry fermented coffee beans with cultures of Saccharomyces cerevisiae showed a higher sensory profile than all other methods. The advantage in using this technique is directly related to the reduction of water consumption by 89%, in the post-harvest stage.
All groups of yeast-fermented coffees presented clustering homogeneity, regardless of the experimental range, indicating a potential of preference of this sensory profile for North American graders.
2Naturals: postharvest processing method; at this stage, the fruits are only washed, where the floaters are separated and the other fruits are taken for drying. In Brazil, this method is commonly known as “via-seco”.
Future research should be carried out to measure the level of acceptance of Brazilian cupping tasters as well as internal and external consumers about the potential for acceptance of wet processed coffee under different methods.
We thank the Federal Institute of Espírito Santo, Coffe Analysis and Research Laboratory―LAPC, Venda Nova do Imigrante Campus, Casa Brasil Coffees for the disposal of the laboratories and cupping tasters for the accomplishment of the experiments and studies and National Council for Scientific and Technological Development (CNPq)―number 469058/2014-5.