Health  Vol.1 No.3 , November 2009
Protective role of buffalo pineal proteins on arsenic-induced oxidative stress in blood and kidney of rats
Abstract: Objective: Exposure to various toxic metals has become an increasingly recognized source of ill- ness in human and animals, worldwide. Arsenic (As) and its compounds cause adverse health effects in animals and humans. Recently, it has been suggested that the pineal gland may also have antioxidants role due to secretary product other than melatonin. With keeping this view, pre-sent investigation tested effect of buffalo (Bubalus bubalis) pineal proteins (PP) on arsenic-induced oxidative stress in RBCs (Red blood cells) and kidney of rats. Methods: Eighteen adult female Wistar rats were grouped into group-I (Control), group-II (Arsenic control), and group-III (Arsenic + Pineal proteins). Experimental rats were given 100 ppm arsenic (p.o.) for 4 weeks alone or along with pineal proteins at a dose of 100 µg/kg body weight (i.p.). Results: Interestingly, arsenic ex-posure led to the stimulation of kidney catalase (CAT) activity, but inhibition of RBCs CAT activ-ity and significantly (P<0.05) increased the RBCs and kidney lipid peroxidation level (LPO). How-ever, arsenic treatment caused depletion of glu- tathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) in kidney tissues. In RBCs, only GR and CAT activity were significantly (P<0.05) declined. These changes were significantly (P<0.05) reversed by PP treatment in arsenic exposed animals. Conclusion: Therefore, present study indicated the significant protecting effect of buf-falo (Bubalus bubalis) PP against arsenic in-duced-oxidative stress through antioxidant de-fense systems in rats.
Cite this paper: nullK. Bharti, V. and Srivastava, R. (2009) Protective role of buffalo pineal proteins on arsenic-induced oxidative stress in blood and kidney of rats. Health, 1, 167-172. doi: 10.4236/health.2009.13027.

[1]   S. J. S. Flora, (1999) Arsenic induced oxidative stress and its reversibility following combined administration of N- acetyl cysteine and meso 2, 3-dimercaptosuccinic acid in rats. Clinical and Experimental Pharmacology Physiology, 26, 865-869.

[2]   S. Bhadauria and S. J. S. Flora, (2007) Response of arse-nic-induced oxidative stress, DNA damage, and metal imbal-ance to combined administration of DMSA and monoisoa-myl-DMSA during chronic arsenic poisoning in rats. Cell Biology and Toxicology, 23, 91-104.

[3]   M. Modi, M. Mittal, and S. J. S. Flora, (2007) Combined administration of selenium and meso-2, 3-dimercapto-succinic acid on arsenic mobilization and tissue oxidative stress in chronic arsenic-exposed male rats. Indian Journal of Pharmacology, 39, 107-114.

[4]   B. Halliwell and J. M. Gutteridge, (1992) Free radicals, antioxidants, and human disease: where are we now. Journal of Laboratory and Clinical Medicine, 119, 598-620.

[5]   B. Halliwell, (2006) Oxidative stress and neurodegenera-tion: where are we known. Journal of Neurochemistry, 97, 1634-1658.

[6]   J. A. Varner, K. F. Jenson, W. Horvath, and R. L. Isaacson, (1998) Chronic administration of aluminum fluoride or sodium fluoride to rats in drinking water: alteration in neuronal and cerebrovascular integrity. Brain Research, 784, 284-298.

[7]   R. J. Reiter, (1991) Pineal melatonin: cell biology of its synthesis and of its physiological interactions. Endocri-nology Review, 12, 151-180.

[8]   R. J. Reiter, D. Melchiorri, E. Sewerynek, and B. Poeggler, (1995) A review of the evidence supporting melatonin’s role as an antioxidant. Journal of Pineal Research, 23, 43-50.

[9]   V. Simonneaux and C. Ribelayga, (2003) Generation of the melatonin endocrine message in mammals: A Review of the complex regulation of melatonin synthesis by norepinephrine, peptides, and other pineal transmitters. Pharmacological Review, 55, 325-395.

[10]   M. Tandon, R. S. Srivastava, S. K. Meur, and M. Saini, (2006) Proteins and peptides present in pineal gland and other brain structures of buffaloes. Indian Journal of Animal Science, 76(5), 383-394.

[11]   V. K. Bharti and R. S. Srivastava, (2009a) Pineal proteins up-regulate specific antioxidant defense systems in the brain. Oxidative Medicine and Cellular Longevity, 2, 88-92.

[12]   M. Ramasamy, (2006) Studies on bubaline pineal pro-teins/peptides below 20 kDa and their immunopotentia-tion in guinea pigs. Ph.D. Thesis. Indian Veterinary Re-search Institute, Izatnagar, India.

[13]   V. Sejian, (2006) Studies on pineal-adrenal relationship in goats (Capra hircus) under thermal stress. Ph.D.Thesis. Indian Veterinary Research Institute, Izatnagar, India.

[14]   V. K. Bharti, (2008) Studies on buffalo (Bubalus bubalis) pineal proteins on fluoride-induced oxidative stress and apoptosis in rats. Ph.D. Thesis. Indian Veterinary Re-search Institute, Izatnagar, India.

[15]   V. K. Bharti and R. S. Srivastava, (2009b) Fluoride-in duced oxidative stress in rat's brain and its amelioration by buffalo (Bubalus bubalis) pineal proteins and melatonin. Biological Trace Element Research, 130, 131-140.

[16]   S. Rehman, (1984) Lead-induced regional lipid peroxida-tion in brain. Toxicology Letter, 21 (3), 333-337.

[17]   J. Sedlak and R. H. Lindsay, (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry, 25(1), 192-205.

[18]   H. K. Prins and J. A. Loos, (1969) In Glutathione, Bio-chemical methods in red cell genetics, edited by J. J. Yunis. Academic Press, New York, 127-129.

[19]   H. U. Bergmayer, (1983) UV method of catalase assay. In Methods of Enzymatic Analysis, 3, Weinheim Deer field Beach, Florida, Bansal, 273.

[20]   M. Madesh and K. A. Balasubramanian, (1998) Microtitre plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Bio-physics, 35, 184-188.

[21]   D. E. Paglia and W. N. Valentine, (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158-169.

[22]   D. M. Goldberg and R. J. Spooner, (1983) Glutathione

[23]   Reductase, J. Bergmeyer, M. Grassi, eds, Methods in En-zymatic Analysis, VCH Weinheim, Germany, 258-265. O. H. Lowry, N. J. Rosebrough, A. I. Farr, and R. J. Randall, (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265-275.

[24]   P. D. Josephy, B. Mannervik, and P. O. Montellano, (1997) Oxidative stress in the erythrocyte. Molecular Toxicology, First Edition, Oxford University Press, New York, USA.