AS  Vol.10 No.2 , February 2019
Genetic Structure of Spinibarbus caldwelli Based on mtDNA D-Loop
Abstract: Spinibarbus caldwelli was an endemic species to China, and its germplasm protection and resources utilization had become more and more concerned. To know its genetic diversity and differentiation, the mitochondrial DNA D-loop was amplified and sequenced for 148 individuals from four regions of Pearl River and Yangtze River Basin. Altogether 9 variable nucleotide sites existed among the aligned sequences of 748 bp, and 8 haplotypes were found within 148 individuals. The average nucleotide diversity (Pi) was high 0.00297, while haplotype diversity (Hd) was 0.706. The average genetic distance was 0.00298, most value occurred between LJ and CL populations, and small value occurred between HJ and QZ populations.
Cite this paper: Yuan, X. , Yang, X. , Ge, H. and Li, H. (2019) Genetic Structure of Spinibarbus caldwelli Based on mtDNA D-Loop. Agricultural Sciences, 10, 173-180. doi: 10.4236/as.2019.102015.

[1]   Fish Research Laboratory, Hubei Institute of Aquatic Biology (1995) Yangtze River Fish. Science Press, Beijing.

[2]   Huckstorf, V. (2013) Spinibarbus caldwelli. The IUCN Red List of Threatened Species 2013: e.T187914A1834637.

[3]   Lin, B.M. and Huang, M.M. (1999) Morphological and Distribution Differences of Other Species of Robinia pseudoacacia and Acridoids and Investigation of Ecological Habits of Robinia pseudoacacia. Scientific Fish Farming, 10, 39.

[4]   Jiang, L.Y., Yu, X.L., Chen, F.Y., Yang, X.M. and Huang, G.H. (2003) Age and Growth of Barbed Cockroaches under Culture Conditions. Journal of Ocean University, 23, 6-13.

[5]   Wen, C.Y., Zou, P.Y., Chen, J.R., Zhong, L.M. and Luo, Q.H. (2005) A Preliminary Study on the Ecology of Light Barbed Hedgehog in the Upper Reaches of the Beijiang River. Journal of Shaoguan University (Natural Science), 26, 79-80, 95.

[6]   Wang, F.Q., Li, Q., Yi, Z.S., Fan, Z.S., Ning, D.R., Yang, X.L., Peng, J.M., Huang, W.Y., Han, W., Zhong, L.M. and Lan, Z.J. (2013) Analysis of Morphological Differences of Different Populations of G. serrata. Anhui Agricultural Sciences, 41, 2464-2465, 2479.

[7]   Tang, Q.Y., Yang, X.P. and Liu, H.Z. (2003) Biogeographical Processes Based on Mitochondrial Cytochrome b Gene in Robinia pseudoacacia. Journal of Hydrobiology, 27, 352-356.

[8]   Huang, Z.Z., Huang, L.T., Lin, X.W. and Ling, W.Z. (2008) Analysis of Mitochondrial DNA Diversity of Black Ridge Thorn Mites. Journal of Ocean University of China (Natural Science Edition), 38, 259-262, 220.

[9]   Xiao, W.H. and Zhang, Y.P. (2000) Genetics and Evolution of Mitochondrial DNA in Fish. Acta Hydrobiologica Sinica, 24, 384-391.

[10]   Fischer, C., Koblmüller, S., Gülly, C., et al. (2013) Complete Mitochondrial DNA Sequences of the Threadfin Cichlid (Petrochromis trewavasae) and the Blunthead Cichlid (Tropheus moorii) and Patterns of Mitochondrial Fenome Evolution in Cichlid Fishes. PLoS One, 8, e67048.

[11]   Bremer, J.R.A., Mejuto, J., Greig, T.W., et al. (1996) Global Population Structure of the Swordfish (Xiphias gladius L.) as Revealed by Analysis of the Mitochondrial DNA Control Region. Journal of Experimental Marine Biology and Ecology, 197, 295-310.

[12]   Yuan, X.P., Yan, L., Xu, S.Y, et al. (2008) Genetic Diversity of Copperfish and Round-Mouthed Copper Fish in the Yangtze River. Chinese Journal of Fisheries Science, 3, 311-315.

[13]   Cann, R.L., Brown, W.M. and Wilson, A.C. (1984) Polymorphic Sites and the Mechanism of Evolution in Human Mitochondrial DNA. Genetics, 106, 479-490.

[14]   Sbisà, E., Tanzariello, F., Reyes, A., Pesole, G. and Saccone, C. (1997) Mammalian Mitochondrial D-Loop Region Structural Analysis: Identification of New Conserved Sequences and Their Functional and Evolutionary Implications. Gene, 205, 125-140.

[15]   Avise, J.C. (1991) Ten Unorthodox Perspectives on Evolution Prompted by Comparative Population Genetic Findings on Mitochondria DNA. Annual Review of Genetics, 25, 45-69.

[16]   Arise, J.C. (1994) Molecular Markers, Natural History and Evolution. Chapman and Hall, New York.

[17]   Bennetts, R.Q., Grady, J.M., Rohde, F.C., et al. (1999) Discordant Patterns of Morphological and Molecular Change in Broadtail Madtoms (Genus Noturus). Molecular Ecology, 8, 1563-1569.

[18]   Zheng, B.R. and Zhang, Y.P. (2002) Genetic Diversity of Mt DNA D-Loop Region Sequence of Yunnan Barb, Waterfowl Fishery, 15-16.

[19]   Liang, H.W., Zou, G.W., Luo, X.Z., Wang, C.Z., Hu, G.F. and Li, Z. (2009) Polymorphism and Phylogenetic Study of mtDNA D-Loop Sequences in Three Chinese Ticks. Journal of Northwest A&F University, 3, 55-59.

[20]   Dai, Y.G., Han, X. and Zhang, X.J. (2010) Sequence Variation and Genetic Diversity of mtDNA D Loop in Liujiang Population of Xiaokoubai Turtle. Journal of Zoology, 45, 115-120.