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 AJAC  Vol.2 No.8 , December 2011
Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy
Abstract: A rapid resolution liquid chromatographic (RRLC) method for the determination of eight polyphenols in to-bacco was developed. Polyphenols were extracted from tobacco samples by methanol/ water in an ultrasonic bath, then subjected to clean up by solid phase extraction. The separation was performed on a 50 × 4.6 mm, 1.8 μm ZORBAX Eclipse XDB-C18 column. Compared with conventional HPLC method, the analysis time for eight polyphenols by RRLC method was reduced from 20 to 5 min without sacrificing resolution, and the sensitivity was improved. This method appears simple, accurate and precious. The relative standard devia-tions (RSD) of overall analysis procedure for eight tobacco polyphenols were less than 2% with the recover-ies ranging from 94% to 107%. This method could be applied to the rapid determination of major polyphe-nols in tobacco with satisfactory results.
Cite this paper: nullF. Xie, A. Yu, D. Hou, H. Liu, L. Ding and S. Zhang, "Rapid and Sensitive Analysis of Eight Polyphenols in Tobacco by Rapid Resolution Liquid Chromatogarphy," American Journal of Analytical Chemistry, Vol. 2 No. 8, 2011, pp. 929-933. doi: 10.4236/ajac.2011.28107.
References

[1]   T. J. Zuo, “Production, Physicology and Biochemistry of Tobacco,” Shanghai Far East Press, Shanghai, 1993.

[2]   L. Bazinet, Y. De Grandpre and A. Porter, “Electromigration of Tobacco Polyphenols,” Separation and Purification Technology, Vol. 41, No. 1, 2005, pp. 101-107. doi:10.1016/j.seppur.2004.05.003

[3]   C. Gong, A. Wang and S. Wang, “Changes of Polyphenols in Tobacco Leaves during the Flue-Curing Process and Correlation Analysis on Some Chemical Components,” Agricultural Sciences in China, Vol. 12, 2006, p. 928932.

[4]   L. Bazinet, Y. De Grandpre and A. Porter, “Enhanced Tobacco Polyphenol Electromigration and Impact on Membrane Integrity,” Journal of Membrane Science, Vol. 254, No. 1-2, 2005, pp. 111-118. doi:10.1016/j.memsci.2004.11.029

[5]   F. J. C. Roe, M. H. Salaman and J. Cohen, “Incomplete Carcinogens in Cigarette Smoke Condensate: Tumour- promotion by a Phenolic Fraction,” British Journal of Cancer, Vol. 13, 1959, pp. 623-633. doi:10.1038/bjc.1959.68

[6]   S. J. Sheen, “Colorimetric Determination of Chlorogenic Acid and Rutin in Tobacco Leaves,” Tobacco Science, Vol. 15, 1971, pp. 116-120.

[7]   F. Li, Q. Liu, W. Cai and X. G. Shao, “Analysis of Scopoletin and Caffeic Acid in Tobacco by GC-MS after a Rapid Derivatization Procedure,” Chromatographia, Vol. 69, No. 7-8, 2009, pp. 743-748. doi:10.1365/s10337-008-0938-2

[8]   H. Cui, C. He and G. Zhao, “Determination of Polyphenols by High-Performance Liquid Chromatography with Inhibited Chemiluminescence Detection,” Journal of Chromatography A, Vol. 855, No. 1, 1999, pp. 171-179. doi:10.1016/S0021-9673(99)00670-6

[9]   Y. Chen, Q. J. Yu, X. Li, Y. Luo and H. Liu, “Extraction and HPLC Characterization of Chlorogenic Acid from Tobacco Residuals,” Separation Science and Technology, Vol. 42, No. 15, 2007, pp. 3481-3492. doi:10.1080/01496390701626677

[10]   J. Gu, X. Zeng, B. Kong, Y. Mao, W. Liu and W. Wei, “Rapid Determination of Polyphenols in Tobacco by MLC,” Chromatographia, Vol. 71, No. 9-10, 2010, pp. 769-774. doi:10.1365/s10337-010-1533-x

[11]   H. Wang, M. Zhao, B. Y. Yang and G. Jiang, “Identification of Polyphenols in Tobacco Leaf and Their Antioxidant and Antimicrobial Activities,” Food Chemistry, Vol. 107, No. 4, 2008, pp. 1399-1406. doi:10.1016/j.foodchem.2007.09.068

[12]   H. Jiang, Y. He, H. Zhao and Y. Hu, “Determination of Chlorogenic Acid and Rutin in Cigarettes by an Improved Capillary Electrophoresis Indirect Chemiluminescence System,” Analytica Chimica Acta, Vol. 512, No. 1, 2004, pp. 111-119. doi:10.1016/j.aca.2004.02.021

[13]   A. Garrido Frenich and J. L. Martínez Vidal, “High- Throughput Determination of Pesticide Residues in Food Commodities by Use of Ultra-Performance Liquid Chro- matography-Tandem Mass Spectrometry,” Analytical and Bioanalytical Chemistry, Vol. 389, No. 6, 2007, pp. 1-14.

[14]   M. Barreda, J. V. Sancho and F. Hernández, “Multiresidue Pesticide Analysis of Fruits by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry,” Analytical and Bioanalytical Chemistry, Vol. 389, 2007, pp. 1765-1771. doi:10.1007/s00216-007-1407-8

[15]   R. Batlle, J. Romero and C. Nerín, “UPLC-MS as a Powerful Technique for Screening the Nonvolatile Contaminants in Recycled PET,” Analytical and Bioanalytical Chemistry, Vol. 388, No. 5-6, 2007, pp. 1031-1038. doi:10.1007/s00216-007-1341-9

[16]   T. Zhang, X. Dong and F. Wu, “Microcolumn High Performance Liquid Chromatography and Spectrometry Method for the Determination of Polyphenols in Tobacco,” Chinese Journal Instrumental Analysis, Vol. 23, 2004, pp. 274-275.

[17]   F. Li, W. Cai and X. Shao, “Analysis of Polyphenols in Tobacco Using Reversed-Phase HPLC,” Chinese Journal of Chromatography, Vol. 25, No. 4, 2007, pp. 565-585.

 
 
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