AJPS  Vol.10 No.1 , January 2019
Seasonal Flavonoid Profile and Kaempferitrin Content in the Leaf Extracts of Bauhinia forficata Subspecies forficata from Two Locations in Southeastern Brazil
Abstract: The leaves of Bauhinia forficata L. (Fabaceae) are used in Brazilian folk medicine mainly as antidiabetic agent. They are rich in polyphenols, and kaempferitrin and other flavonoids are currently considered as basic chemical criteria for the quality and the effectiveness of extracts and phytopharmaceuticals derived from them. Aiming to expand the scope of current data of the chemical profile of B. forficata subsp. forficata, the present study employed HPLC-DAD and HPLC-DAD-ESI/MS to compare the flavonoid profile and kaempferitrin content in the leaves of specimens collected seasonally during one year, in two different locations in Southeastern Brazil: Rio de Janeiro (RJ) and São Paulo (SP) states. The data showed a variation in the flavonoid profile and in the kaempferitrin content depending on the place of collection and the season of the year. The target compound, kaempferitrin, was found to be only a minor constituent in RJ (0.21 - 1.02 μg/mg), and was mostly absent or occurred as trace in SP location (0.00 - 0.12 μg/mg). Some degree of similarity was observed in the flavonoid profiles according to the region of collection. In agreement with some published reports, the low and quite variable kaempferitrin content as well the wide variability of the flavonoid profiles brings into question the dubious matter of using these compounds as chemical markers for this medicinal species.
Cite this paper: Tostes, J. , Siani, A. , Monteiro, S. , Melo, V. , Costa, J. and Valente, L. (2019) Seasonal Flavonoid Profile and Kaempferitrin Content in the Leaf Extracts of Bauhinia forficata Subspecies forficata from Two Locations in Southeastern Brazil. American Journal of Plant Sciences, 10, 208-220. doi: 10.4236/ajps.2019.101016.

[1]   Lorenzi, H. and Matos, F.J.A. (2008) Plantas Medicinais no Brasil: Nativas e exóticas. Instituto Plantarum de Estudos da Flora LTDA, Nova Odessa.

[2]   Cechinel, V.F. (2009) Chemical Composition and Biological Potential of Plants from the Genus Bauhinia. Phytotherapy Research, 23, 1347-1354.

[3]   Nogueira, A.C. and Sabino, C.V.S. (2012) Revision of the Genus Bauhinia Addressing the Scientific Aspects of Species Bauhinia forficata Link and Bauhinia variegata L. of Interest to the Pharmaceutical Industry. Revista Fitos, 7, 77-84.

[4]   Trojan-Rodrigues, M., Alves, T.L.S., Soares, G.L.G. and Ritter, M.R. (2012) Plants Used as Antidiabetics in Popular Medicine in Rio Grande do Sul, Southern Brazil. Journal of Etnopharmacology, 139, 155-163.

[5]   Batista, P.N., Oliveira, T.C., Leal, F.R., Batista, A.N., Medeiros, M.G.F. and Nunes, L.C.C. (2013) The Influence of Using Bauhinia forficata Link in Glycemic, Lipid and Toxicological Profile in in Vivo Experimental Models: A Systematic Review. Journal of Medicinal Plants Research, 7, 2343-2348.

[6]   Silva, M.I.G., Melo, C.T.V., Vasconcelos, L.F., Carvalho, A.M.R. and Sousa, F.C.F. (2012) Bioactivity and Potential Therapeutic Benefits of Some Medicinal Plants from the Caatinga (Semi-Arid) Vegetation of Northeast Brazil: A Review of the Literature. Revista Brasileira de Farmacognosia, 22, 193-207.

[7]   Brasil (2009) RENISUS—Relação Nacional de Plantas Medicinais de Interesse ao SUS. Espécies vegetais.

[8]   Salgueiro, A.C.F., Leal, C.Q., Bianchini, M.C., Prado, I.O., Mendez, A.S.L., Puntel, R.L., Folmer, V., Soares, F.A., Avila, D.S. and Puntel, G.O. (2013) The Influence of Bauhinia forficata Link subsp. pruinosa Tea on Lipid Peroxidation and Non-Protein SH Groups in Human Erythrocytes Exposed to High Glucose Concentrations. Journal of Ethnopharmacology, 148, 81-87.

[9]   Silva, F.R.M.B., Szpoganicz, B., Pizzolatti, M.G., Willrich, M.A.V. and de Sousa, E. (2002) Acute Effect of Bauhinia forficata on Serum Glucose Levels in Normal and Alloxan-Induced Diabetic Rats. Journal of Ethnopharmacology, 83, 33-37.

[10]   Jorge, A.P., Horst, H., de Sousa, E., Pizzolatti, M.G. and Silva, F.R.M.B. (2004) Insulinomimetic Effects of Kaempferitrin on Glycemia and on 14C-Glucose Uptake in rat Soleus Muscle. Chemico-Biological Interactions, 149, 89-96.

[11]   Sousa, E., Zanatta, L., Seifriz, I., Creezynski-Pasa, T.B., Pizzolatti, M.G., Szpoganiez, B. and Silva, F. (2004) Hypoglycemic Effect and Antioxidant Potential of Kaempferol-3,7-O-(alpha)-dirhamnoside from Bauhinia forficata Leaves. Journal of Natural Products, 67, 829-832.

[12]   da Silva, K.L., Biavatti, M.W., Leite, S.N., Yunes, R.A., Delle Monache, F. and Cechinel Filho, V. (2000) Phytochemical and Pharmacognostic Investigation of Bauhinia forficata Link (Leguminosae). Zeitschrift für Naturforschung C, 55, 478-480.

[13]   de Sousa, E., Zanatta, L., Seifriz, I., Creczynski-Pasa, T.B., Pizzolatti, M.G., Szpoganicz, B. and Silva, F.R.M.B. (2004) Hypoglycemic Effect and Antioxidant Potential of Kaempferol-3,7-O-(α)-dirhamnoside from Bauhinia forficata Leaves. Journal of Natural Products, 67, 829-832.

[14]   Pinheiro, T.S.D.B., Johansson, L.A.P., Pizzolatti, M.G. and Biavatti, M.W. (2006) Comparative Assessment of Kaempferitrin from Medicinal Extracts of Bauhinia forficata Link. Journal of Pharmaceutical and Biomedical Analysis, 41, 431-436.

[15]   Engel, I.C., Ferreira, R.A., Cechinel-Filho, V. and Meyre-Silva, C. (2008) Quality Control of Drugs from Bauhinia forficata Link (Fabaceae). Revista Brasileira de Farmacognosia, 18, 258-264.

[16]   Pizzolatti, M.G., Cunha Jr., A., Szpoganicz, B., Sousa, E.D., Braz-Filho, R. and Schripsema, J. (2003) Flavonoids Glycosides from Leaves and Flowers of Bauhinia forficata (Leguminosae). Química Nova, 26, 466-469.

[17]   Farias, L.D.S. and Mendez, A.S. (2014) LC/ESI-MS Method Applied to Characterization of Flavonoids Glycosides in B. forficata subsp. pruinosa. Quimica Nova, 37, 483-486.

[18]   Ferreres, F., Gil-Izquierdo, A., Vinholes, J., Silva, S.T., Valentão, P. and Andrade P.B. (2012) Bauhinia forficata Link Authenticity Using Flavonoids Profile: Relation with Their Biological Properties. Food Chemistry, 134, 894-904.

[19]   Farag, M.A., Sakna, S.T., El-fiky, N.M., Shabana, M.M. and Wessjohann, L.A. (2015) Phytochemical, Antioxidant and Antidiabetic Evaluation of Eight Bauhinia L. Species from Egypt Using UHPLC-PDA-qTOF-MS and Chemometrics. Phytochemistry, 119, 41-50.

[20]   González-Mujica, F. (2011) Antihiperglycaemic Activity of Bauhinia megalandra. In: Rasooli, I., Ed., Phytochemicals-Bioactivities and Impact on Health, InTech, Croatia, 333-356.

[21]   Frankish, N., Menezes, F.S., Mills, C. and Sheridan, H. (2010) Enhancement of Insulin Release from the β-Cell Line INS-1 by an Ethanolic Extract of Bauhinia variegata and Its Major Constituent Roseoside. Planta Medica, 76, 995-997.

[22]   Toloza-Zambrano, P., Avello, M. and Fernández, P. (2015) Determinación de rutina y trigonelina en extractos de hojas de Bauhinia forficata subsp. pruinosa y evaluación del efecto hipoglicemiante en humanos. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 14, 1.

[23]   Miceli, N., Buongiorno, L.P., Celi, M.G., Cacciola, F., Dugo, P., Donato, P., Mondello, L., Bonacorsi, I. and Taviano, M.F. (2016) Role of the Flavonoid-Rich Fraction in the Antioxidant and Cytotoxic Activities of Bauhinia forficata Link. (Fabaceae) Leaves Extract. Natural Product Research, 30, 1229-1239.

[24]   Da Cunha, A.M., Menon, S., Menon, R., Couto, A.G., Bürger, C. and Biavatti, M.W. (2010) Hypoglycemic Activity of Dried Extracts of Bauhinia forficata Link. Phytomedicine, 17, 37-41.

[25]   Peixoto-Sobrinho, T.J.S., Gomes, T.L.B., Cardoso, K.C.M., Albuquerque, U.P. and Amorim, E.L.C. (2012) Teor de flavonóides totais em produtos contendo pata-de-vaca (Bauhinia L.) comercializados em farmácias de Recife/PE. Revista Brasileira de Plantas Medicinais, 14, 586-591.

[26]   Marques, G.S., Leao, W.F., Lyra, M.A.M., Peixoto, M.S., Monteiro, R.P.M., Rolim, L.A., Xavier, H.S., Neto, P.J.R. and Soares, L.A.L. (2013) Comparative Evaluation of UV/VIS and HPLC Analytical Methodologies Applied for Quantification of Flavonoids from Leaves of Bauhiniaforficata. Revista Brasileira de Farmacognosia, 23, 51-57.

[27]   Santos, M., Fortunato, R.H. and Spotorno, V.G. (2018) Analysis of Flavonoid Glycosides with Potential Medicinal Properties on Bauhinia uruguayensis and Bauhinia forficata Subspecies pruinosa. Natural Product Research, 1-5.

[28]   Arigony, A.L.V. (2005) Determinação química e biológica de Bauhinia forficata Link subespécie pruinosa (pata-de-vaca-Leguminosae). MSc Thesis, Universidade Federal do Rio Grande do Sul, Porto Alegre.

[29]   Ardila, J.A., Funari, C.S., Andrade, A.M., Cavalheiro, A.J. and Carneiro, R.L. (2015) Cluster Analysis of Commercial Samples of Bauhinia spp. Using HPLC-UV/PDA and MCR-ALS/PCA without Peak Alignment Procedure. Phytochemical Analysis, 26, 367-373.

[30]   Paixão, D. (2010) Desenvolvimento por planejamento fatorial e validação de metodologia analítica utilizando extração em fase sólida e cromatografia líquida de alta eficiência na quantificação do flavonoide campferitrina em Uncaria guianensis (Rubiaceae). MSc Thesis, Universidade Federal do Rio de Janeiro.

[31]   Mabry, T.J., Markham, K.R. and Thomas, M.B. (1970) The Systematic Identification of Flavonoids. Springer, Berlin.

[32]   Marques, G.S., Monteiro, R.P.M., Leao, W.F., Lyra, M.A.M., Peixoto, M.S., Rolim-Neto, P.J., Xavier, H.S. and Soares, L.A.L. (2012) Evaluation of Procedures for Spectrophotometric Quantification of Total Flavonoids in Leaves of Bauhinia forficata Link. Química Nova, 35, 517-522.

[33]   Marques, G.S., Lyra, M.A.M., Peixoto, M.S., Monteiro, R.P.M., Leao, W.F., Xavier, H.S., Soares, L.A.L. and Rolim, N.P.J. (2012) Phytochemical Characterization and Physical Chemistry of the Leaves of Bauhinia forficata Link Collected in Two Brazilian Regions. Revista de Ciências Farmacêuticas Básica e Aplicada, 33, 57-62.

[34]   Gobbo-Neto, L. and Lopes, N.P. (2007) Plantas medicinais: Fatores de influência no conteúdo de metabólitos secundários. Química Nova, 30, 374-381.

[35]   Dicke, M. and Hilker, M. (2003) Induced Plant Defences: From Molecular Biology to Evolutionary Ecology. Basic and Applied Ecology, 4, 3-14.

[36]   Winkel-Shirley, B. (2002) Biosynthesis of Flavonoids and Effects of Stress. Current Opinion in Plant Biology, 5, 218-223.

[37]   Sahoo, N., Manchikanti, P. and Dey, S. (2010) Herbal Drugs: Standards and Regulation. Fitoterapia, 81, 462-471.

[38]   Fortunato, R.H. (1986) Revision del genero Bauhinia (Cercideae, Caesalpinioidea, Fabaceae) para la Argentina. Darwiniana, 27, 527-557.

[39]   Fortunato, R.H. (1996) Bauhinia affinis (Fabaceae) uma nueva cita para la flora Argentina. Darwiniana, 34, 405-409.

[40]   Vaz, A.M.S. and Tozzi, A.M.G. (2005) Synopsis of Bauhinia sect. Pauletia (Cav.) DC. (Leguminosae: Caesalpinioideae: Cercideae) in Brazil. Brazilian Journal of Botany, 28, 477-491.

[41]   Moranza, H.G., Scanavaca Junior, L., Mataqueiro, M.I. and Ferraz, G. (2017) Chronic Administration of Bauhinia forficata Link Reduced Exploratory Activity without Affecting Blood Glucose in Healthy Rats. Plant, 5, 27-32.