AJAC  Vol.2 No.8 , December 2011
Optimization of Dry Ashing of Whole Blood Samples for Trace Metal Analysis
Abstract: Dry ashing is an established method. Ashing whole blood samples are, however, often difficult to carry out with significant sample loss, and the procedure is not well documented. A new procedure has been devel- oped and optimized to dry-ash whole blood samples for trace metal analyses. The procedure reduces both the dry-ashing time by more than two thirds and sample loss. The ashed sample can be readily used in subse- quent, simultaneous or individual analysis of several metals by ICP-OES, as demonstrated in the analysis of a whole blood sample. The new procedure is simple, inexpensive, and faster than the established method.
Cite this paper: nullS. Bragg and Z. Xue, "Optimization of Dry Ashing of Whole Blood Samples for Trace Metal Analysis," American Journal of Analytical Chemistry, Vol. 2 No. 8, 2011, pp. 979-983. doi: 10.4236/ajac.2011.28114.

[1]   D. T. Burns, “Precursors to and Evolution of Elemental Organic Tube Combustion Analysis over the Last Two Hundred Years,” Analytical Procedures, Vol. 30, No. 6, 1993, pp. 272-275. doi:10.1039/ap9933000272

[2]   J. Cholak and D. M. Hubbard, “Spectrographic Determi- nation of Beryllium in Biological Material and in Air,” Analytical Chemistry, Vol. 20, No. 1, 1948, pp. 73-76. doi:10.1021/ac60013a020

[3]   J. Sysalova and V. Spevackova, “A Study of Sample Min- eralization Methods for Arsenic Analysis of Blood and Urine by Hydride Generation and Graphite Furnace Atomic Absorption Spectrometry,” Central European Journal of Chemistry, Vol. 1, No. 2, 2003, pp. 108-120. doi:10.2478/BF02479263

[4]   J. Versieck and L. Vanballenberghe, “Determination of Tin in Human Blood Serum by Radiochemical Neutron Activetion Analysis,” Analytical Chemistry, Vol. 63, No. 11, 1991, pp. 1143-1146. doi:10.1021/ac00011a016

[5]   G. Cobo, M. Gomez, C. Camara and M. A. Palacios, “Determination of Fluoride in Complex Liquid Matrices by Electrothermal Atomic Absorption Spectrometry with in-Furnace Oxygen-Assisted Ashing,” Microchimica Acta, Vol. 110, No. 1-3, 1993, pp. 103-110. doi:10.1007/BF01243991

[6]   L. Vesterberg and T. Bergstrom, “Determination of Cad- mium in Blood by Use of Atomic Absorption Spectroscopy with Crucibles―and a Rational Procedure for Dry- Ashing,” Clinical Chemistry, Vol. 23, 1977, pp. 555-559.

[7]   G. Nise and O. Vesterberg, “Blood Lead Determination by Flameless Atomic Absorption Spectroscopy,” Clinica Chimica Acta, Vol. 84, No. 1-2, 1978, pp. 129-136. doi:10.1016/0009-8981(78)90485-0

[8]   C. J. Price, P. L. Strong, F. J. Murray and M. M. Goldberg, “Blood Boron Concentrations in Pregnant Rats Fed Boric Acid Throughout Gestation,” Reproductive Toxicology, Vol. 11, No. 6, 1997, pp. 833-842. doi:10.1016/S0890-6238(97)00067-1

[9]   K. Bukhave, A. S?rensen and M. Hansen, “A Simplified Method for Determination of Radioactive Iron in Whole- Blood Samples,” Journal of Trace Elements in Medicine and Biology, Vol. 15, No. 1, 2001, pp. 56-58. doi:10.1016/S0946-672X(01)80027-4

[10]   W. Jiang, H. Tong, Z.-C. Liu, K. Wang, B.-C. Chen and C.-H. Li, “Determination of Whole Blood Lead by Dry Ashing-GFAAS,” Chinese Journal of Health Laboratory Technology (Zhongguo Weisheng Jianyan Zazhi), Vol. 18, No. 3, 2008, pp. 464-465.

[11]   L. Cao and Y. He, “Determination of Blood Lead and Cadmium Levels by Dry-Ash Graphite Furnace Atomic Absorption Spectrometry,” Occupation and Health (Zhiye Yu Jiankang), Vol. 24, No. 23, 2008, pp. 2536-2537.

[12]   J. Titze, H. Krause, H. Hecht, P. Dietsch, J. Rittweger, R. Lang, K. A. Kirsch and K. F. Hilgers, “Reduced Osmotically Inactive Na Storage Capacity and Hypertension in the Dahl Model,” American Journal of Physiology―Re- nal Physiology, Vol. 283, No. 1, 2002, pp. F134-F141.

[13]   K. Drábek and J. Kalousková, “Comparison of Recoveries of Inorganic and Organic Incorporated 75-Se by Four Mineralization Methods of Biological Material,” Journal Radioanalytical and Nuclear Chemistry, Vol. 119, No. 2, 1987, pp. 119-129. doi:10.1007/BF02169840

[14]   J. Titze, J. Rittweger, P. Dietsch, H. Krause, K. H. Schwind, K. Engelke, R. Lang, K. A. Kirsch, F. C. Luft and K. F. Hilgers, “Hypertension, Sodium Retention, Cal- cium Excretion and Osteopenia in Dahl Rats,” Journal of Hypertension, Vol. 22, 2004, pp. 803-810. doi:10.1097/00004872-200404000-00024

[15]   J. Bian, X. Zhang and W. Ni, “Determination of Aluminum in Flour Products by Microwave Digestion/Dry Ashing-Spectrophotometry,” Chemistry Research (Huaxue Yanjiu), Vol. 22, No. 2, 2011, pp. 61-64.

[16]   V. M. Tomovic, L. S. Petrovic, M. S. Tomovic, Z. S. Kevresan, M. R. Jokanovic, N. R. Dzinic and A. R. Despotovic, “Cadmium Levels of Kidney from 10 Different Pig Genetic Lines in Vojvodina (Northern Ser- bia),” Food Chemistry, Vol. 129, No. 1, 2011, pp. 100- 103. doi:10.1016/j.foodchem.2011.04.032

[17]   C. R. Brown, K. G. Haynes, M. Moore, M. J. Pavek, D. C. Hane, S. L. Love, R. G. Novy, J. C. Miller Jr., “Stability and Broad-Sense Heritability of Mineral Content in Potato: Zinc,” American Journal of Potato Research, Vol. 88, No. 3, 2011, pp. 238-244. doi:10.1007/s12230-011-9188-1

[18]   N. Sogabe, R. Maruyama, O. Baba, T. Hosoi and M. Goseki-Sone, “Effects of Long-Term Vitamin K1 (Phylloquinone) or Vitamin K2 (Menaquinone-4) Supplementation on Body Composition and Serum Parameters in Rats,” Bone, Vol. 48, No. 5, 2011, pp. 1036-1042. doi:10.1016/j.bone.2011.01.020

[19]   A. L. R. M. Rossete, J. M. T. Carneiro, H. H. Batagello, J. G. G. Oliveira and J. A. Bendassolli, “Spectrophotometric Determination of Sulfur in Plants Using Dry Ash Oxidation and Alkaline Oxidizers,” Quimica Nova, Vol. 34, No. 2, 2011, pp. 341-343. doi:10.1590/S0100-40422011000200030

[20]   J. Vogl, M. Rosner and W. Pritzkow, “Development and Validation of a Single Collector SF-ICPMS Procedure for the Determination of Boron Isotope Ratios in Water and Food Samples,” Journal of Analytical Atomic Spectrometry, Vol. 26, No. 4, 2011, pp. 861-869. doi:10.1039/c0ja00220h

[21]   L. Yong, K. C. Armstrong, R. N. Dansby-Sparks, N. A. Carrington, J. Q. Chambers and Z. Xue, “Quantitative Analysis of Trace Chromium in Blood Samples. Combination of the Advanced Oxidation Process with Catalytic Adsorptive Stripping Voltammetry,” Analytical Chemistry, Vol. 78, No. 21, 2006, pp. 7582-7587. doi:10.1021/ac060707p

[22]   R. N. Dansby- Sparks, R.-Z. Ouyang and Z.-L. Xue, “Optical and Electro-Chemical Sol-Gel Sensors for Inorganic Species,” Science in China Series B: Chemistry, Vol. 52, No. 11, 2009, pp. 1777-1788. doi:10.1007/s11426-009-0278-6

[23]   R. Bock, “A Handbook of Decomposition Methods in Analytical Chemistry,” International Textbook Company, Glasgow, 1979.

[24]   Analytical Methods Committee, “Methods for the De- struction of Organic Matter,” Analyst, Vol. 85, 1960, pp. 643-656. doi:10.1039/an9608500643

[25]   P. F. E. Van Montfort, J. Agterdenbos and B. A. H. G. Juette, “Determination of Antimony and Tellurium in Hu- man Blood by Microwave Induced Emission Spectrome- try,” Analytical Chemistry, Vol. 51, No. 9, 1979, pp. 1553-1557. doi:10.1021/ac50045a045

[26]   A. Ferrando, N. Green, K. Barnes and B. Woodward, “Microwave Digestion Preparation and ICP Determination of Boron in Human Plasma,” Biological Trace Element Research, Vol. 37, 1993, pp. 17-25. doi:10.1007/BF02789398

[27]   D. C. Harris, “Quantitative Chemical Analysis,” Mac- millan, 2003.

[28]   P. Mader, J. Száková and E. Curdová, “Combination of Classical Dry Ashing with Stripping Voltammetry in Trace Element Analysis of Biological Materials: Review of Literature Published after 1978,” Talanta, Vol. 43, No. 4, 1996, pp. 521-534. doi:10.1016/0039-9140(95)01793-3

[29]   P. Tidehag, G. Hallmans, K. Wing, R. Sj?str?m, G. ?Gren, E. Lundin and J. Zhang, “A Comparison of Iron Absorp- tion from Single Meals and Daily Diets Using Radio Fe (Fe, 59Fe),” British Journal of Nutrition, Vol. 75, 1996, pp. 281-289. doi:10.1079/BJN19960130

[30]   G. Middleton and R. E. Stuckey, “The Preparation of Bio- logical Material for the Determination of Trace Metals. Part I. A Critical Review of Existing Procedures,” Analyst, Vol. 78, 1953, pp. 532-542. doi:10.1039/an9537800532

[31]   T. T. Gorsuch, “Radiochemical Investigations on the Re- covery for Analysis of Trace Elements in Organic and Biological Materials, Report to the Analytical Methods Committee by the Society’s First Analytical Chemistry Research Scholar,” Analyst, Vol. 84, 1959, pp.135-173. doi:10.1039/an9598400135

[32]   R. E. Thiers, “Contamination in Trace Analysis and Its Control,” Methods of Biochemical Analysis, Vol. 5, 1957, pp. 273-335. doi:10.1002/9780470110218.ch6