AiM  Vol.8 No.12 , December 2018
Profile of Turbidity and Glucose Formation from Underutilised Wild, Edible Bean during In-Vitro Gastro Intestinal Digestion and Fermentation
Abstract: Fermentation takes place throughout the gastrointestinal tract of all animals, but the intensity and products of fermentation depend on number and types microbes, which are generally highest in the large bowel. Large intestinal epithelial cells do not produce digestive enzymes, but contain huge numbers of bacteria which have the enzymes to digest and utilize many substrates. The seeds of beans (Otili, Feregede, Pakala and Oloyin) analyzed in this present study contain indigestible fraction called dietary fiber which helps to maintain functioning of the digestive system. Fermentation of indigestible fraction (IF) of these beans was mimicked through in-vitro method which leads to biochemical changes in the samples. During this experiment, increase in acidity and turbidity was observed. The glucose concentration decreases with some exceptions, such as Pakala fermented by Lactobacillus acidophilus which had the value of 6.260 mmol/L at 6 hr and increased to 6.616 mmol/L after 18 hours fermentation, Otili fermented by various microorganisms which had its turbidity increased by 50%. Lactobacillus acidophilus fermenting Pakala had the highest glucose concentration during the fermentation period. The increase in turbidity could be as a result of increase in microbial flora or production of metabolites, such as glucose. The approach followed here may be used as a predictive model to assess the metabolic implications of food substrates present in the traditional Nigerian orphan beans.
Cite this paper: Omodara, T. , Awoyinka, O. , Oladele, F. , Aina, O. and Olaiya, M. (2018) Profile of Turbidity and Glucose Formation from Underutilised Wild, Edible Bean during In-Vitro Gastro Intestinal Digestion and Fermentation. Advances in Microbiology, 8, 994-1004. doi: 10.4236/aim.2018.812067.

[1]   Hermsdorff, H.H.M., Zulet, M.A., Abete, I. and Martínez, J.A. (2011) A Legume-Based Hypocaloric Diet Reduces Proinflammatory Status and Improves Metabolic Features in Overweight/Obese Subjects. European Journal of Nutrition, 50, 61-69.

[2]   Marques, F.Z., Nelson, E., Chu, P.-Y., Horlock, D., Fiedler, A., Ziemann, M. and El-Osta, A. (2017) High-Fiber Diet and Acetate Supplementation Change the Gut Microbiota and Prevent the Development of Hypertension and Heart Failure in Hypertensive Mice Clinical Perspective. Circulation, 135, 964-977.

[3]   Wikoff, W.R., Anfora, A.T., Liu, J., Schultz, P.G., Lesley, S.A., Peters, E.C., et al. (2009) Metabolomics Analysis Reveals Large Effects of Gut Microflora on Mammalian Blood Metabolites. Proceedings of the National Academy of Sciences of the United States of America, 106, 3698-3703.

[4]   Hood-Niefer, S.D., Warkentin, T.D., Chibbar, R.N., Vandenberg, A. and Tyler, R.T. (2012) Effect of Genotype and Environment on the Concentrations of Starch and Protein in, and the Physicochemical Properties of Starch from, Field Pea and Fababean. Journal of the Science of Food and Agriculture, 92, 141-150.

[5]   Louis, P., El Aidy, S., van den Abbeele, P., van de Wiele, T. and Kleerebezem, M. (2013) Intestinal Colonization: How Key Microbial Players Become Established in This Dynamic Process: Microbial Metabolic Activities and the Interplay between the Host and Microbes. BioEssays, 35, 913-923.

[6]   Sharon, G., Garg, N., Debelius, J., Knight, R., Dorrestein, P.C. and Mazmanian, S.K. (2014) Specialized Metabolites from the Microbiome in Health and Disease. Cell Metabolism, 20, 719-730.

[7]   Richards, L.B., Li, M., vanEsch, B.C.A.M., Garssen, J. and Folkerts, G. (2016) The Effects of Short Chain Fatty Acids on the Cardiovascular System. Pharma Nutrition, 4, 68-111.

[8]   Gibson, G.R., Macfarlane, G.T., Beatty, E. and Cummings, J.H. (1992) Estimation of Short-Chain Fatty Acid Production from Protein by Human Intestinal Bacteria Based on Branched-Chain Fatty Acid Measurements. FEMS Microbiology Ecology, 101, 81-88.

[9]   Shanahan, F. (2002) The Host-Microbe Interact within the Gut. Best Practice & Research: Clinical Gastroenterology, 16, 915-931.

[10]   Santacruz, A., Collado, M.C., Garcia-Valdes, L., Segua, M.T., Martin-Lagos, J.A., Anjos, T., Marti-Romero, M., Lopez, R.M., Florido, J., Campoy, C. and Sanz, Y. (2010) Gut Microbiota Composition Is Associated with Body Weight, Weight Gain and Biochemical Parameters in Pregnant Women. British Journal of Nutrition, 104, 83-92.

[11]   Roediger, W.E. (1980) Role of Anaerobic Bacteria in the Metabolic Welfare of the Colonic Mucosa in Man. Gut, 21, 793-798.

[12]   Russell, W.R., Hoyles, L., Flint, H.J. and Dumas, M.E. (2013) Colonic Bacterial Metabolites and Human Health. Current Opinion in Microbiology, 16, 246-254.

[13]   Luis, V., Sean, H. and Samantha, C. (2015) Metabolic Interactions in the Gastrointestinal Tract (GIT): Host, Commensal, Probiotics, and Bacteriophage Influences. Microorganisms, 3, 913-932.

[14]   Hamer, H.M., Jonkers, D., Venema, K., Vanhoutvin, S., Troost, F.J. and Brummer, R.J. (2008) Review Article: The Role of Butyrate on Colonic Function. Alimentary Pharmacology & Therapeutics, 27, 104-119.

[15]   Geier, M.S., Butler, R.N. and Howarth, G.S. (2007) Inflammatory Bowel Disease: Current Insights into Pathogenesis and New Therapeutic Options; Probiotics, Prebiotics and Synbiotics. International Journal of Food Microbiology, 115, 1-11.

[16]   Flint, H.J., Duncan, S.H., Scott, K.P. and Louis, P. (2007) Interactions and Competition within the Microbial Community of the Human Colon: Links between Diet and Health. Environmental Microbiology, 9, 1101-1111.

[17]   Danese, S. and Fiocchi, C. (2006) Etiopathogenesis of Inflammatory Bowel Diseases. World Journal of Gastroenterology, 12, 4807-4812.

[18]   Breuer, R.I., Soergel, K.H., Lashner, B.A., Christ, M.L., Hanauer, S.B., Vanagunas, A., Harig, J.M., Keshavarzian, A. and Robinson, M. (1997) Short-Chain Fatty Acid Rectal Irrigation for Left-Sided Ulcerative Colitis: A Randomized, Placebo-Controlled Trial. Gut, 40, 485-491.

[19]   Bajka, B.H., Clarke, J.M., Cobiac, L. and Topping, D.L. (2008) Butyrylated Starch Protects Colonocyte DNA against Dietary Protein-Induced Damage in Rats. Carcinogenesis, 29, 2169-2174.

[20]   Aparicio-Fernandez, X., Yousef, G.G., Loarca-Pina, G., De-Mejia, E. and Lila, M.A. (2005) Characterization of Polyphenolic in the Seed Coat of Black Jamapa Bean (Phaseolus vulgaris). Journal of Agricultural and Food Chemistry, 53, 4615-4622.

[21]   Benoit, S.C., Kemp, C.J., Elias, C.F., Abplanalp, W., Herman, J.P., Migrenne, S., Lefevre, A.L., Cruciani-Guglielmacci, C., Magnan, C., Yu, F., Niswender, K., Irani, B.G., Holland, W.L. and Clegg, D.J. (2009) Palmitic Acid Mediates Hypothalamic Insulin Resistance by Altering PKC-θ Subcellular Localization in Rodents. Journal of Clinical Investigation, 119, 2577-2587.

[22]   Mentor-Marcel, R.A., Bobe, G., Barrett, K.G., Young, M.R., Albert, P.S., Bennink, M.R., et al. (2009) Inflammation-Associated Serum and Colon Markers as Indicators of Dietary Attenuation of Colon Carcinogenesis in ob/ob Mice. Cancer Prevention Research, 2, 60-69.

[23]   Riviere, A., Marija, S. and Luc-De, V. (2016) Bifidobacteria and Butyrate Producing Colon Bacteria: Importance and Strategies for Their Stimulation in the Human Gut. Frontiers in Microbiology, 7, 979.

[24]   Baron, E., Peterson, L. and Finegold, S. (1994) Non-Fermentative Gram-Negative Bacilli and Coccobacilli. In: Bailey & Scott’s Diagnostic Microbiology, 9th Edition, Mosby-Year Book, St. Louis, 386-405.

[25]   Benachinmardi, K.K., Padmavathy, M., Malini, J. and Naveneeth, B. (2014) Prevalence of Non Fermenting Gram-Negative Bacilli and Their in Vitro Susceptibility Pattern at a Tertiary Care Teaching Hospital. Journal of the Scientific Society, 41, 162-166.

[26]   Blondel-Hill, E., Henry, E.A. and Speert, D.P. (2007) Pseudomonas. In: Manual of Clinical Microbiology, 9th Edition, American Society for Microbiology, Washington DC, 734-748.

[27]   Chang, S., Cui, X., Guo, M., Tian, Y., Xu, W., Huang, K. and Zhang, Y. (2017) Insoluble Dietary Fiber from Pear Pomace Can Prevent High-Fat Diet Induced Obesity in Rats Mainly by Improving the Structure of Gut Microbiota. Journal of Microbiology and Biotechnology, 27, 856-867.

[28]   Campos-Vega, R., Reynoso-Camacho, R., Pedraza-Aboytes, G., Acosta-Gallegos, J.A., Guzman Maldonado, S.H., Paredes-Lopez, O., Oomah, B.D. and Loarca-Piña, G. (2009) Chemical Composition and in Vitro Polysaccharide Fermentation of Different Beans (Phaseolus vulgaris L.). Journal of Food Science, 74, T59.

[29]   Zamora-Gasga, V.M., álvarez-Vidal, C., Montalvo-González, E., Loarca-Piña, G., Vázquez-Landaverde, P.A., Bello-Pérez, L.A., Tovar, J. and Sáyago-Ayerdi, S.G. (2018) Gut Metabolites Associated with pH and Antioxidant Capacity during in Vitro Colonic Fermentation of Mexican Corn Products. Cereal Chemistry, 95, 399-410.

[30]   Falony, G., Vlachou, A., Verbrugghe, K. and De Vuyst, L. (2006) Cross-Feeding between Bidobacterium longum BB536 and Acetate-Converting, Butyrate Producing Colon Bacteria during Growth on Oligofructose. Applied and Environmental Microbiology, 72, 7835-7841.

[31]   Mathers, J.C. and Annison, E.F. (1993) Stoichiometry of Polysaccharide Fermentation in the Large Intestine. In: Samman, S. and Annison, G., Eds., Dietary Fibre and Beyond-Australian Perspectives, Nutrition Society of Australia Occasional Publications, Vol. 1, 123-135.

[32]   Mazotto, A.M., Rodrigues-Coelho, R.R., Lage-Cedrola, S.M., Lima, M.F., Couri, S., Paraguai de Souza, E. and Vermelho, A.B. (2011) Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feathers as Substrate in a Submerged Fermentation. Enzyme Research, 2011, Article ID: 523780.

[33]   Hosoi, T., Ametani, A., Kiuchi, K. and Kaminogawa, S. (2000) Improved Growth and Viability of Lactobacilli in the Presence of Bacillus subtilis (Natto), Catalase, or Subtilisin. Canadian Journal of Microbiology, 46, 892-897.