JBM  Vol.6 No.12 , December 2018
Free Radical Activity as Diagnostic and Prognostic Criteria in Solid Tumors and Their Therapy
Abstract: Blood plasma of 39 cancer patients who had not previously undergone anti-tumor treatment, and 21 patients with recurrent epithelial tissues cancer before treatment and after the first course of chemotherapy were investigated. The treatment effectiveness of patients with relapse was evaluated throughout the year. The control group was the blood plasma of 14 healthy people. Free radical activity and oxidative modification of proteins were assessed in blood plasma. In malignant neoplasms of epithelial tissues, free radical activity and oxidative modification of plasma proteins are significantly higher. At the initial stages of carcinogenesis, the oxidative modification level of plasma proteins increases both due to aldehyde and ketone carbonyl derivatives. In the terminal stage of malignant tumors, an increase in the oxidative modification of proteins occurs due to aliphatic ketone dinitrophenyl hydrazones, which are the markers of protein aggregation characteristic of the late stages of oxidative stress. In case of an objective response to polychemotherapy, there is a significant decrease in free radical plasma activity after the first course of therapy and a significant increase in carbonyl derivatives recorded at a wavelength of 230 nm, which can serve as a marker of its effectiveness.
Cite this paper: Erlykina, E. , Obukhova, L. and Kopytova, T. (2018) Free Radical Activity as Diagnostic and Prognostic Criteria in Solid Tumors and Their Therapy. Journal of Biosciences and Medicines, 6, 1-12. doi: 10.4236/jbm.2018.612001.

[1]   Semiglazov, V.V. and Topuzov, E.E. (2009) Breast Cancer. MEDpress-Inform, Moscow, 176. [In Russian]

[2]   Aschele, C., Lonardi, S. and Monfardini, S. (2002) Thymidylate Synthase Expression as a Predictor of Clinical Response to Fluoropyrimidine-Based Chemotherapy in Advanced Colorectal Cancer. Cancer Treatment Reviews, 28, 27-47.

[3]   Libra, M., Navolanic, P.M., Talamini, R. and Toffoli, G. (2004) Thymidylate Synthetase mRNA Levels Are Increased in Liver Metastases of Colorectal Cancer Patients Resistant to Fluoropyrimidine-Based Chemotherapy. BMC Cancer, 1, 11-17.

[4]   Shain, А.А. (2004) Oncology. Iz-datel’skii centr Akademiya, Tyumen. [In Russian]

[5]   Miki, J., Furusato, B., Li, H., Gu, Y., Takahashi, H., Egawa, S., Sesterhenn, I.A., McLeod, D.G., Srivastava, S. and Rhim, J.S. (2007) Identification of Putative Stem Cell Markers, CD 133, and CXCR_4, in hTERT-Immortalised Primary Nonmalignant and Malignant Tumor_Derived Human Prostate Epithelial Cell Lines and in Prostate Cancer Specimens. Cancer Research, 67, 3153-3161.

[6]   Naito, Y., Lee, M.-C., Kato, Y., Nagai, R. and Yoshikazu, Y. (2010) Oxidative Stress Markers. Anti-Aging Medicine, 7, 36-44.

[7]   Jones, L.A., Holmes, J.C. and Seligman, R.B. (1956) Spectrophotometric Studies of Some 2,4-Dinitrophenylhydrazones. Analytical Chemistry, 2, 191-198.

[8]   Ho, E., Galougahi, K.K., Liu, C.-C., Bhindi, R. and Figtree, G.A. (2013) Biological Markers of Oxidative Stress: Applications to Cardiovascular Research and Practice. Redox Biology, 1, 483-491.

[9]   Kuz'mina, Е.I., Nelyubin, А.S. and Shchennikova, М.К. (1983) Application of Induced Chemoluminescence for Assessment of Free Radical Reactions in Biological Substrates. Mezhvuzovskii sbornik biokhimii i biofiziki mikroorganizmov. Gorky, 179-183. (In Russian)

[10]   Dubinina, Е.Е., Burmistrov, S.О., Khodov, D.А. and Porotov, I.G. (1995) Oxidative Modification of Human Blood Plasma Proteins, the Method of Its Determination. Voprosy meditsinskoi khimii, 1, 24-26. (In Russian)

[11]   Dubinina, Е.Е. (2006) Products of Oxygen Metabolism in Functional Activity of Cells (Life and Death, Creation and Destruction). Fiziologicheskie i kliniko-boikhimicheskie aspekty. SPb.: Meditsinskaya pressa, 440. (In Russian)

[12]   Sarsour, E.H., Kumar, M.G., Chaudhuri, L. and Goswami, P.C. (2009) Redox Control of the Cell Cycle in Health and Disease. Antioxidants & Redox Signaling, 12, 2985-3011.

[13]   Chakraborti, S., Mandal, M., Das, S., Mandal, A. and Chakraborti, T. (2003) Regulation of Matrix Metalloproteinases: An Overview. Molecular and Cellular Biochemistry, 1-2, 269-285.

[14]   Browatzki, M., Larsen, D., Pfeiffer, C.A., Gehrke, S.G., Schmidt, J., Kranzhofer, A., Katus, H.A. and Kranzhofer, R. (2005) Angiotensin II Stimulates Matrix Metalloproteinase Secretion in Human Vascular Smooth Muscle Cells via Nuclear Factor-KappaB and Activator Protein 1 in a Redox-Sensitive Manner. R.J. Vasc. Res, 5, 415-423.

[15]   Shugalei, I.V. (2000) Chain Process of Peroxidation of Proteins—A Suitable Model for Investigation of Destructive Ability of Active Forms of Oxygen. Russkii zhurnal VICh/SPID i rodstvennye problem, 1, 77-78. (In Russian)

[16]   Pasechnik, I.N. (2004) Oxidative Stress as a Component of Formation of Critical States in Surgical Patients. (In Russian)

[17]   Squier, T.C. (2001) Oxidative Stress and Protein Aggregation during Biological Aging. Experimental Gerontology, 9, 1539-1550.

[18]   Lambeth, J.D. (2007) Nox Enzymes, ROS, and Chronic Disease: An Example of Antagonistic Pleiotropy. Free Radical Biology & Medicine, 3, 332-347.

[19]   Piroddi, M., Depunzio, I., Calabrese, V., Mancuso, C., Aisa, C.M., Binaglia, L., Minelli, A., Butterfield, A.D. and Galli, F. (2007) Oxidatively-Modified and Glycated Proteins as Candidate Pro-Inflammatory Toxins in Uremia and Dialysis Patients. Amino Acids, 4, 573-592.

[20]   Winterbourn, C.C. (2000) Biomarkers of Myeloperoxidase-Derived Hypochlorous Acid Free Radic. Biology and Medicine, 5, 403-409.

[21]   Ryabov, G.А., Aziziv, G.А. and Pasechnik, I.N. (2002) Oxidative Stress and Endogenous Intoxication of Patients in Critical States. Vestnik Intensivnoi Terapii, 4, 4-7. (In Russian)

[22]   Tolochko, Z.S. and Spiridonov, V.К. (2010) Oxidative Modification of Proteins in Blood of Rats at Destruction of Capsaicin-Sensitive Nerves and Change in the Level of Nitrogen Oxide. Rossiskii fiziologicheskii zhurnal im. I.М. Sechenova, 1, 77-84. (In Russian)

[23]   Muravleva, L.Е., Molotov-Luchanskii, V.B. and Klyuev, D.А. (2010) Oxidative Modification of Proteins: Problems and Prospects of Investigation. Fundament. Issled, 1, 74-78. (In Russian)

[24]   Blokhin, Yu.D. (2004) Phenotype of Multiple Drug Stability of Tumor Cells Due to Destruction of the Program of Cell Death. Vestn. Ramn., 12, 16-20. (In Russian)