Graphene oxide is one of the carbon allotropes with a Nano size that gives a high surface area and the ample of oxygenic functional groups that gives the ability to react and join with active or catalysis material  . This modification on graphene oxide makes it able to save and generate energy; furthermore, these functional groups give it porous structure, so it could be used as current collector or doped material for electrodes in supercapacitor or lithium batteries  . This distinctive nano structure of graphene oxide enables us to use it in various applications like electronics, fuel cells, supercapacitor and sensors. In principle, we can’t use graphene oxide in applications that require electric conductivity. This is due to poverty of graphene oxide to electrical conductivity  . Graphene oxide is similar to structure of graphite layer, but the plane of carbon atoms in graphene oxide is heavily decorated by oxygen-containing groups  , GO has many exogenous functional groups such as epoxy (bridging oxygen atoms), hydroxy in the basal plane, carboxyl and carbonyl moieties lining the nanosheet edge  .
In this study, new Nano compound derivative is synthesized from graphene oxide and can be used in several application corrosion inhibitors, solar cell, biological, electronics, etc.
2-amine pyridine, graphite, Hydrogen peroxide, Potassium permanganate, Sodium nitrate, DCC, DAMP, 7-hydroxy-5-sulfobenzoic acid from Fluka, Hydrochloric acid, Sulphuric acid from BDH.
3. Synthesis of Graphene Oxide GO, GAP and GSO
3.1. Preparation of Graphene Oxide (GO)
Graphene oxide was prepared according to Hummers  method, 2 g of graphite was added to cool 50 ml concentrated H2SO4 and stirred in an ice bath for 15 minutes. 1 g of sodium nitrate and 6 g of potassium permanganate were added to above solution and stirred in an ice bath for 2 hours. The ice-bath removed and the temperature of the mixture was kept at 35˚C in water path for 30 minutes. After that, the mixture became pasty (deep red-brown in color). 50 ml of deionized water was then added to above mixture. The temperature then raised to 90˚C - 98˚C. The above mixture was diluted by addition 250 ml warm deionized water. Following this, 30% H2O2 (~30 ml) was added till the solution turned bright yellow. The graphite oxide powder was dried at 40˚C for 24 h   shown in Figure 1.
3.2. Preparation of GAP
The GAP derived from GO, mix of grapheme oxide (0.5 g) with 2-amino pyridine (9.4 g, 9 mmole) dissolved in DMF 100 ml sonicated for 1 h to form homogeneous solution, then added DCC (2.06 g, 10 mmole), DMAP (1.22 g, 10 mmole). Then the mixture was stirred at 45˚C for 24 hours. To quench the reaction, an equimolar amount of HCl was added to the solution to neutralize
Figure 1. Chemical equation of preparation (GO).
DMAP, after the reaction finish, the product was filter, then black powder was dried  shown in Figure 2.
3.3. Preparation of GSO
The as-synthesized GO has many carboxylic groups within its molecular structure which is perfect for esterification, First, GO (0.5 g), with 7-hydroxy-5-sulfobenzoic acid were dissolved in DMF, 100 ml at the room temperature then DCC (2.06 g, 10 mmol) and DMAP (1.22 g, 10 mmol) sonicated for 1 h to form homogeneous solution. The mixture was stirred at 45˚C for 24 hours. To quench the reaction, an equimolar amount of HCl was added to the solution to neutralize DMAP the product was filter, then black powder was dried  shown in Figure 3.
Figure 2. Chemical equation of preparation (GAP).
Figure 3. Chemical equation of preparation (GSO).
4. Characterization Graphene Oxide GO, GAP and GSO
4.1. FTIR for GO, GAP and GSO
FTIR analysis was used to identify functional groups present in the GO, GAP and GSO, Figures 4(a)-(c) show the FTIR spectrum of, GO, GAP and GSO powders respectively, the GO spectrum shows the peak at 3386.39 cm−1 due to O-H stretching vibration, 1724.36 cm−1 was strong C=O stretching band, and peak at 16,228 cm−1, 1378.85 cm−1 can be attributed to C=C stretching of aromatic ring, C-OH bending respectively, the characteristic peak at 1029.8 cm−1 due to C-O epoxy group  .
Figure 4. FTIR spectrum of (a) GO powder (b) GAP powder (c) GSO powder.
Figure 4(b) shows GAP the peak at 3434.6 cm−1 for stretching OH groups. The presence of absorption bands at 3420.4 cm−1 N-H band, 1639.2 cm−1 due to C=O amide stretching vibration ,the peak at 1559.17, 1415.49, 1122 .37 cm−1 attributed to C=C aromatic ring, C=N and C-N stretching, also 1029.8 cm−1 due to C-O epoxy group 797.421 cm−1 due to pyridine ring  .
Figure 4(c) shows GSO the peak at 3419.17 cm−1 for stretching OH groups, 1643.05 cm−1 due to C=O aster stretching vibration, the peak at 1569.77 is attributed to C=C aromatic ring, the peaks 1350.69, 1150.53 cm−1 assigned to the asymmetric and symmetric of S=O bands, also 1127.19, 1024.02, 953.627 cm−1 due to C-O, epoxy, S-O bending  .
4.2. H1NMR for GO, GAP and GSO
H1-NMR spectra of graphene oxide (GO) show the characteristic peaks at (a) at (8.08) ppm is assigned to the proton of −COOH groups, (7.50) ppm is singlet assigned to the protons of OH phenolic groups, the singlet that appear at (7) ppm is assigned to the phenyl groups. All of them is weak and broad because the deshielding of the electron density that yielded by the hydrogen bonding between the layers of GO graphene oxide that leads to reduce the chemical shift of the protons. The singlet, (b) (2.5) ppm is belong to the solvent (c) The peak at (3.75) ppm is singlet assigned to the −CH groups that also s group to effect chemical shift  (Figure 5(a)).
Figure 5(b) GAP (2.50) ppm is assigned to the solvent, (a) (3.50) ppm is assigned to the −NH groups. (b) (7.00 - 7.50) ppm a multiplet are assigned to the pyridine rings (c) (8.08) ppm a multiplet due to H-atoms of phenyl groups of GO, and (d) the peaks at (11) ppm is assigned to the phenolic groups in GO because the shielding is reduced from OH groups by the amidation of GO with 2-amino pyridine  .
Figure 5(c) GSO, the peaks at (2.5, 3.5) ppm are assigned to the solvent, (a) (7.0, 7.5, 8.0 and 8.5) ppm due to H-atoms of phenyl groups of both sulphonate ring and GO, of, and (b) (c) the peaks at (12 and 13.0) ppm are singlets assigned to the H of phenolic (in sulphonate ring and GO) of and carboxylic acid groups, respectively  .
4.3. X-Ray Diffraction (XDR) of GO, GAP and GSO
In Figure 6(a), the X-Ray Diffraction (XRD) of grapheme oxide shows a large interlayer spacing equal to 8.06 A˚ at the position (2θ = 10.97˚) disappearance of the peak at 26˚ due to completely oxidized after the chemical oxidation and exfoliation  .
Figure 6(b) GAP shows that many peaks appear (2θ = 10.97) at d-spacing 8.06 indicate to graphene oxide and other peaks 2θ = 32.28, 38.78, 49.58, 76.58, 59.61, 77.28 and 78.90 have to d-spacing 2.77, 2.31, 1.83, 1.54, 1.24, 1.23 and 1.21, respectively attributed to functionalized 2-amino pyridine at graphene oxide also intermediate layer  .
Figure 5. H1 NMR spectrum of (a) GO (b) GAP (c) GSO.
Figure 6. XRD of (a) GO, (b) GAP, (c) GSO.
Figure 6(c) of GSO shows that many peaks appear (2θ = 10.97) at d-spacing 8.06 indicate to graphene oxide and other peaks 2θ = 32.31, 34.02, 38.80, 48.96, 49.63, 57.49, 59.62, 62.10, 65.43 and 67.68 have to d-spacing 2.77, 2.63, 1.86, 1.83, 1.60, 1.55, 1.49, 1.42 and 1.38, respectively attributed to functionalized 7-hydroxy-5-sulfobenzoic acid at graphene oxide also intermediate layer  .
The Diffraction patterns of X-ray to prepared organic compound particles size is calculated using (Debye-Scherer) equation 
where; D: Particles size, λ: X-ray wave length (nm), β: Half width at half maximum (HWHM), K: is s related hape factor, normally taken as 0.9. θ is X-ray angle. From this equation the particle size of grapheme oxide (GO) (16 nm) but to calculated average particle size to prepared organic compounds GAP (23.22 nm), GSO (40.74 nm). Also calculated particles size using (Williamson-Hall) (W-H) equation 
where £ micro strain of particles, λ: X-ray wave length (nm), β: Half width at half maximum (HWHM), K: is s related hape factor, normally taken as 0.9. θ is X-ray angle where calculated to depend on XRD θ to compound in Figure 6(b) and Figure 6(c). The calculated particles size from graphic between (sinθ) on x-axis, (βcosθ) on y-axis, D calculated by intercept (Kλ/D), Figure 7, from this equation particles size of GAP (5.82 nm), and the micro strain £ value was calculated from the Equation (2) GSO (13.69 nm), GAP micro strain (−0.00494), GSO micro strain (−0.00356). The values, sinθ and βcosθ, are shown in Table 1.
Table 1. The value sinθ and (βcosθ) GAP, GSO.
Figure 7. Scheme Williamson-Hall to GAP, GSO.
4.4. Field Emission Scanning Electron Microscopy (FESEM)
The FESEM of graphene oxide (GO) very sharp edges and flat surface the dark gray areas consist of several layers of sheets also kinked and wrinkled areas  (Figure 8(a)), but GAP appeared relatively coarse very sharp edges and flat surface (Figure 8(b)), GSO the re-stacked layers and crumpling, kinked and wrinkled areas   (Figure 8(c)).
Graphene oxide is synthesized by Hummer method and derivative GAP is prepared by reaction graphene oxide with 2-amino pyridine, GSO when graphene oxide reacts with 2-hydroxy-5-sulfobenzoic acid, graphene oxide and their derivatives characterization by FTIR, H1NMR and XRD. The patterns of XRD calculated particles size using two equations (Debye-Scherer) and (Williamson-Hall) compared between (Debye-Scherer) equation and (William-son-Hall), and the average particles size using (Debye-Scherer) equation high and (Williamson-Hall) to the attributed width peaks to particles size and Internal emotion, which is small when using powders. Also graphene oxide and their derivatives characterize by FESEM observed dark gray areas consist of several layers of sheets and light grey areas represent few layers.
Figure 8. FESEM of (a) GO, (b) GAP, (c) GSO.