Back
 AiM  Vol.8 No.11 , November 2018
Nutritional Requirements for Mycelial Growth of Ophiocordyceps sinensis on Agar Media
Abstract: Ophiocordyceps sinensis has been used as one of the most valuable traditional Chinese Medication. This fungus parasitizes larva of Hepialus armoricanus, and converts each larva into a sclerotium form, in which the fruit body grows. Due to the geographical limitation, where O. sinensis can only be found in Himalayas region, the natural resources are limited and very expensive. This research aims to compare the growth-rate of O. sinensis mycelia with different ingredients mix with agar media using one-factor-at-a-time method. This research demonstrated the mycelial growth-rate with different carbon sources, including monosaccharide (Fructose, Glucose), disaccharide (Maltose, Sucrose), and polysaccharide (Dextrin, Malt extract), complex organic nitrogen sources, including beef extract, yeast extract, whey protein, and soy protein, and eight different carbon to nitrogen ratios. The objective of this research is to find out the suitable carbon and organic complex nitrogen sources and ratio for the O. sinensis solid cultivation. As results, O. sinensis grew best with disaccharides comparing to the other types of carbon sources. Furthermore, O. sinensis preferred whey protein in contrast to other organic complex nitrogen sources. As for the carbon to nitrogen ratios, an optimal ratio of 18:1 was observed. Based on those experiments, carbon source shows a greater influence for the mycelial growth. Hence many different types of grains and cereals would be great candidates as the main ingredients for the O. sinensis solid cultivation.
Cite this paper: Huang, P. and Ohga, S. (2018) Nutritional Requirements for Mycelial Growth of Ophiocordyceps sinensis on Agar Media. Advances in Microbiology, 8, 821-829. doi: 10.4236/aim.2018.811054.
References

[1]   Lei, W., Zhang, G., Peng, Q. and Liu, X. (2015) Development of Ophicordyceps sinensis through Plant-Mediated Interkingdon Host Colonization. International Journal of Molecular Science, 16, 17482-17493.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581204
https://doi.org/10.3390/ijms160817482


[2]   Tuli, H.S., Sandhu, S.S. and Sharma, A.K. (2014) Pharmacological and Therapeutic Potential of Cordyceps with Special Reference to Cordycepin. 3 Biotech, 4, 1-12.
https://www.ncbi.nlm.nih.gov/pubmed/28324458
https://doi.org/10.1007/s13205-013-0121-9


[3]   Chioza, A. and Ohga, S. (2014) A Review on Fungal Isolates Reported as Anamorphs of Ophiocordyceps sinensis. Journal of Mycology, 2014, Article ID: 913917.
https://www.hindawi.com/journals/jmy/2014/913917/

[4]   Zhang, Y.J., Liu, X.Z. and Wang, M. (2008) Cloning, Expression, and Characterization of Two Novel Cuticle-Degrading Serine Proteases from the Entomopathogenic Fungus Cordyceps sinensis. Research in Microbiology, 159, 462-469.
https://www.sciencedirect.com/science/article/pii/S0923250808000594?via%3Dihub
https://doi.org/10.1016/j.resmic.2008.04.004


[5]   Zou, Z.W., Liu, X. and Zhang, G.R. (2010) Revision of Taxonomic System of the genus Hepialus (Lepidoptera, Hepialidae) Currently Adopted in China. Journal of Hunan University Science Technology, 5, 114–120.

[6]   Pegler, D.N., Yao, Y.J. and Li, Y. (1994) The Chinese “Caterpillar Fungus”. Mycologist, 8, 3-5.
https://www.sciencedirect.com/science/article/pii/S0269915X09806708
https://doi.org/10.1016/S0269-915X(09)80670-8


[7]   Dong, C. and Yao, Y. (2012) Isolation, Characterization of Melanin Derived from Ophiocordyceps sinensis, and Entomogenous Fungus Endemic to the Tibetan Plateau. Journal of Bioscience and Bioengineering, 113, 474-479.
https://www.sciencedirect.com/science/article/pii/S1389172311004956?via%3Dihub
https://doi.org/10.1016/j.jbiosc.2011.12.001


[8]   Chen, X.X., Chen, P., Huang, Y., Mo, M.H., Xu, J. and Zheng, S.C. (2016) The Mechanisms of Pharmacological Activities of Ophiocordyceps sinensis Fungi. Phytotherapy Research, 30, 1572-1583.
https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.5673
https://doi.org/10.1002/ptr.5673

[9]   Zhou, X.W., Gong, Z.G., Lin, L.J., Su, Y. and Tang, K. (2009) Cordyceps Fungi: Natural Products, Pharmacological Functions and Developmental Products. Journal of Pharmacy and Pharmacology, 61, 279-291.
https://onlinelibrary.wiley.com/doi/abs/10.1211/jpp.61.03.0002
https://doi.org/10.1211/jpp.61.03.0002

[10]   Jiang, Y. and Yao, Y.J. (2002) Names Related to Cordyceps sinensis Anamorph. Mycotaxon, 84, 245-254.
https://www.researchgate.net/publication/288606447_Names_related_to_Cordyceps_sinensis_anamorph

[11]   Yao, Y. (2004) Conservation and Rational Use of the Natural Resources of Cordyceps sinensis. Science News, 15, 28-29.
https://www.researchgate.net/publication/285058628_Conservation_and_rational_use_of_the_natural_resources_of_Cordyceps_sinensis

[12]   Kuzyakov, Y., Liu, S., Schleuss, P.M., Zamanian, K. and Zarebanadkouki, M. (2017) Degradation of Tibetan Grasslands: Consequences for Carbon and Nutrient Cycles. Agriculture Ecosystems & Environment, 252, 93-104.
https://www.researchgate.net/publication/320701070_Degradation_of_Tibetan_grasslands_Consequences_for_carbon_and_nutrient_cycles

[13]   Li, W.C., Qiu, J.J. and Qiu, F. (2011) Study on Sustainable Utilization of Resources of Cordyceps sinensis in Nagqu Tibet. Ecology Economy, 6, 112-114.

[14]   Chioza, A. and Ohga, S. (2013) Mycelial Growth of Paecilomyces hepiali in Various Agar Media and Yield of Fruit Bodies in Rice Based Media. Advances in Microbiology, 3, 529-536.
https://file.scirp.org/Html/4-2270245_40127.htm
https://doi.org/10.4236/aim.2013.37071

[15]   Xerox Corporation (2005) Three Key Paper Properties. Xerox Corporation.
https://www.xerox.com/downloads/usa/en/t/ThreeKeyPaperPropertiesWhitePaper.pdf

[16]   Ge, F., Gui, L., Li, C.R. and Fan, M.Z. (2009) Studies on Solid-State Fermentation Condition of Hirsutella sinensis Anamorph of Cordyceps sinensis. Journal of Biology, 26, 22-25.
http://en.cnki.com.cn/Article_en/CJFDTOTAL-SWXZ200903010.htm

[17]   Chioza, A. and Ohga, S. (2014) Effects of Hot-Water Extract of Paecilomyces hepiali on Hypertension Parameters in Spontaneously Hypertensive Rats. Advances in Microbiology, 4, 436-443.
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=46854
https://doi.org/10.4236/aim.2014.48048


[18]   Chioza, A. and Ohga, S. (2014) A Comparative Study on Chemical Composition and Pharmacological Effects of Paecilomyces hepiali and Wild Ophiocordyceps sinensis. Advances in Microbiology, 4, 839-848.
http://www.scirp.org/journal/PaperInformation.aspx?PaperID=50113
https://doi.org/10.4236/aim.2014.412093


[19]   Choua1, S.M., Lai, J.W., Hong, T.W., Lai, J.Y., Tsai, S.H. and Chen, Y.H. (2014) Synergistic Property of Cordycepin in Cultivated Cordyceps militaris-Mediated Apoptosis in Human Leukemia Cells. Phytomedicine, 21, 1516-1524.
https://www.sciencedirect.com/science/article/pii/S0944711314003006?via%3Dihub

[20]   Yue, G.G.L., Lau, C.B.S., Fung, K.P.F., Leung, P.C. and Ko, W.H. (2008) Effects of Cordyceps sinensis, Cordyceps militaris and Their Isolated Compounds on Ion Transport in Calu-3 Human Airway Epithelial Cells. Journal of Ethnopharmacology, 117, 92-101.
https://www.sciencedirect.com/science/article/pii/S0378874108000524?via%3Dihub
https://doi.org/10.1016/j.jep.2008.01.030


 
 
Top