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 AiM  Vol.8 No.10 , October 2018
Study on the Cultivation of Agaricus blazei (Almond Mushroom) Grown on Compost Mixed with Selected Agro-Residues
Abstract: The Agaricus blazei strain (KUMB 1221) from Forest Production Control Laboratory, Kyushu University was grown on the basal media of compost mixed with selected agro-residues (sawdust, woodchips and corncob) in the ratio of 25%, 50%, 75% and 100% (by weight). The data was collected on the colony diameter of mycelial growth, days required for spawn run (colonization), days required for primordial formation, whiteness measurement, days required for fruit body formation, number, size and fresh weight of mushrooms, biological efficiency (BE) and mushroom production (MP). It was observed that the mycelial growth showed two types of mycelia according the level of mycelial density; compact (C) and somewhat compact (SC). The fastest spawn run (17 days) was found in woodchips (50%) among all the different treatments while the least spawn run took 26 days in corncob (75%). Sawdust substrates promoted longer days for primordial formation and fruit body development, if compared with woodchips substrates and corncob substrates. With regard to yield, 100%, 75% and 50% mixture with compost were superior to 25% mixture with compost in each group of selected agro-residues and it indicated that adding more compost gave the increased yield. Interestingly, it was noted that mushroom size on compost (100%) was double to the mushroom size of other treatments. In conclusion, it was clearly showed that compost (100%), woodchips (25%) and corncob (25%) could produce better yields among all treatments, and it was also possible to obtain acceptable yields of good quality almond mushroom using main substrates of compost mixed with different agro-residues at various concentrations.
Cite this paper: Win, T. and Ohga, S. (2018) Study on the Cultivation of Agaricus blazei (Almond Mushroom) Grown on Compost Mixed with Selected Agro-Residues. Advances in Microbiology, 8, 778-789. doi: 10.4236/aim.2018.810051.
References

[1]   Linde, G.A., Alessandra, T., Nunes, R., Domingues, G., Figueiredo, E.F., Santin, K.C. and Colauto, N.B. (2014) Mushroom Acceptability and Consumption Intention for the Main Mushrooms Produced in Brazil. Postgraduate Program in Biotechnology Applied to Agriculture, Paranaense University, Umuarama, 627-632.

[2]   Mizuno, T. (2002) Medicinal Properties and Clinical Effects of Culinary-Medicinal Mushroom Agaricus blazei Murrill (Agaricomycetideae) (Review). International Journal of Medicinal Mushrooms, 4, 35-44.
https://doi.org/10.1615/IntJMedMushr.v4.i4.30

[3]   Silva, C.F., Azevedo, R.S., Braga, C., Silva, R. and Dias, E.S. (2009) Microbial Diversity in a Bagasse-Based Compost Prepared for the Production of Agaricus brasiliensis. Brazilian Journal of Microbiology, 40, 590-600.
https://doi.org/10.1590/S1517-83822009000300023

[4]   Gregori, A., Pahor, B., Glaser, R. and Pohleven, F. (2008) Influence of Carbon Dioxide, Inoculum Rate, Amount and Mixing of Casing Soil on Agaricus blazei Fruiting Bodies Yield. Acta Agriculturae Slovenica, 91, 371-378.
https://doi.org/10.2478/v10014-008-0017-2

[5]   Stijve, B.T., Angela, M., Amazonas, L.D.A. and Florestas, E. (2004) The Royal Sun Agaricus or Himematsutake—An Update. Mushroom Science, 22, 47-50.

[6]   Llarena-Hernández, C.R., Largeteau, M.L., Ferrer, N., Regnault-Roger, C. and Savoie, J.M. (2014) Optimization of the Cultivation Conditions for Mushroom Production with European Wild Strains of Agaricus subrufescens and Brazilian Cultivars. Journal of the Science of Food and Agriculture, 94, 77-84.
https://doi.org/10.1002/jsfa.6200

[7]   De Siqueira, F.G., Dias, E.S., Silva, R., Tokuda, E. and Rinker, D.L. (2009) Cultivation of Agaricus blazei ss. Heinemann Using Different Soils as Source of Casing Materials. Victoria, 66, 827-830.

[8]   Stamets, P. (1993) Growing Gourmet and Medicinal Mushrooms. Ten Speed Press, Berkeley.

[9]   Wang, Q., Li, B.B., Li, H. and Han, J.R. (2010) Yield, Dry Matter and Polysaccharides Content of the Mushroom Agaricus blazei Produced on Asparagus Straw Substrate. Scientia Horticulturae, 125, 16-18.
https://doi.org/10.1016/j.scienta.2010.02.022

[10]   Horm, V. and Ohga, S. (2008) Potential of Compost with Some Added Supplementary Materials on the Development of Agaricus blazei Murill. Journal of the Faculty of Agriculture, Kyushu University, 53, 417-422.

[11]   Pokhrel, C.P. and Ohga, S. (2007) Cattle Bedding Waste Used as a Substrate in the Cultivation of Agaricus blazei Murill. Journal of the Faculty of Agriculture, Kyushu University, 52, 295-298.

[12]   Chu, J.N., Young, C.C., Tan, C.C., Wu, S.P. and Young, L. (2012) Improvement of Productivity and Polysaccharide-Protein Complex in Agaricus blazei. Pesquisa Agropecuária Brasileira, 47, 96-102.
https://doi.org/10.1590/S0100-204X2012000100013

[13]   De Mendonca, M., Kasuya, M.C., Cadorin, A. and Vieira, A.J. (2005) Shiitake Cultivation Part II Mushroom for Better Life.

[14]   Lentinula, S. and Spawn, E. (2003) Supplementation of Sugarcane Bagasse with Rice Bran and Sugarcane Molasses for Shiitake. Brazilian Journal of Microbiology, 34, 61-65.
https://doi.org/10.1590/S1517-83822003000100013

[15]   Pardo-Giménez, A., Zied, D.C. and Pardo-González, J.E. (2010) Utilización de compost agotado de champiñón como capa de coberturas en nuevos ciclos de producción. Pesquisa Agropecuaria Brasileira, 45, 1164-1171.
https://doi.org/10.1590/S0100-204X2010001000016

[16]   Stamets, P. (2000) Growing Gourmet and Medicinal Mushrooms. 3rd Edition, Ten Speed Press, Berkeley, 208-216.

[17]   Colauto, N.B., Da Eira, A.F. and Linde, G.A. (2012) Cryopreservation of Agarius blazei in Liquid Nitrogen Using DMSO as Cryoprotectant. Bioscience Journal, 28, 1034-1037.

[18]   Colauto, N.B., da Silveria, A.R., da Eira, A.F. and Linde, G.A. (2011) Production Flush of Agaricus blazei on Brazilian Casing Layers. Brazilian Journal of Microbiology, 42, 616-623.

[19]   Da Eira, A.F. (2003) Cultivo do cogumelo medicinal Agaricus blazei (Murril) ss. Heinemann ou Agaricus brasiliensis (Wasser et al.) CPT.
https://books.google.co.jp/books?id=WZFIAAAACAAJ

[20]   De Andrade, M.C.N., Kopytowski Filho, J., Minhoni, M.T.D.A., Coutinho, L.N. and Figueiredo, M.B. (2007) Productivity, Biological Efficiency, and Number of Agaricus blazei Mushrooms Grown in Compost in the Presence of Trichoderma sp. and Chaetomium olivacearum Contaminants. Brazilian Journal of Microbiology, 38, 243-247. https://doi.org/10.1590/S1517-83822007000200010

[21]   Kerrigan, R.W. (2005) Agaricus subrufescens, a Cultivated Edible and Medicinal Mushroom, and Its Synonyms. Mycologia, 97, 12-24.
https://doi.org/10.1080/15572536.2006.11832834

[22]   Kim, H.H., Na, J.G., Chang, Y.K., Chun, G.T., Lee, S.J. and Jeong, Y.H. (2004) Optimization of Submerged Culture Conditions for Mycelial Growth and Exopolysaccharides Production by Agaricus blazei. Journal of Microbiology and Biotechnology, 14, 944-951.

[23]   Wang, H., Fu, Z. and Han, C. (2013) The Medicinal Values of Culinary-Medicinal Royal Sun Mushroom (Agaricus blazei Murrill), 2013.

[24]   Zied, D.C., Pardo-Giménez, A., de Almeida Minhoni, M.T., Villas Boas, R.L., Alvarez-Orti, M. and Pardo-González, J.E. (2012) Characterization, Feasibility and Optimization of Agaricus subrufescens Growth Based on Chemical Elements on Casing Layer. Saudi Journal of Biological Sciences, 19, 343-347.
https://doi.org/10.1016/j.sjbs.2012.04.002

 
 
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