JBM  Vol.6 No.10 , October 2018
Heparin Sodium Was Prepared from Pig Intestinal Mucosa by Dialysis and Spray Drying
Abstract: A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method; the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.
Cite this paper: Shu, S. , Mi, Q. , Yang, C. , Bao, C. , Wang, Z. and Niu, J. (2018) Heparin Sodium Was Prepared from Pig Intestinal Mucosa by Dialysis and Spray Drying. Journal of Biosciences and Medicines, 6, 5-11. doi: 10.4236/jbm.2018.610002.

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