OJAS  Vol.8 No.4 , October 2018
Seroprevalence and Associated Risk Factors of Peste des Petits Ruminants among Sheep and Goats in Kassala State, Sudan
Peste des petits ruminant (PPR) is a contagious disease of small ruminants caused by a virus that belongs to the genus Morbillivirus of the family Paramyxoviridae. This study aimed to determine the seroprevalence of PPR disease in sheep and goats and its associated risk factors in Kassala State, Eastern Sudan. Across sectional study was conducted during the period from 30th August to 25th November 2015. The study was carried out using a structured questionnaire survey and a total of 918 blood samples were collected from apparently healthy unvaccinated sheep and goats in different localities in State of Kassala. A total of 546 sheep and 372 goats were tested for specific antibodies to nucleoprotein (NP) by competitive enzyme linked immunosorbent assay (cELISA). The apparent overall prevalence of PPR antibodies in Kassala was 58.2% while the true prevalence was calculated to be 61.3%. The apparent prevalence in sheep and goats was 68.1% and 43.5% respectively. Univariate analysis showed that the risk factors had significant associations with a cELISA positive status: locality, species, age, breed, husbandry system, housing mode, animals movement (p = 0.000) and animals sharing pasture and water (p = 0.003), while sex and newly introduced animals were not significant risk factors (p = 0.771) (p = 0.050) respectively. Factors found that significantly associated (p < 0.05) with increased odds of being cELISA positive in multivariate analysis were localities, species, age and newly introduced animals. The prevalence differed between localities and was the highest in the River Atbara (84.0%) locality, whereas it was lowest in Delta North (29.0%). No significant difference was observed among the sexes. However, the prevalence differed in different age groups and was 52.25% in animals of less than six months old; 49.3% were between seven months and two years old and 65.5% were above two years old. In different husbandry systems, the prevalence was 47.9%, 73.0% and 49.2% in intensive, open grazing and pastoral systems respectively. Housing type effects were also observed; the highest prevalence was in animals housed in metal fence (83.3%). The movement pattern showed significant effect, where the prevalence was the highest (81.3%) in animals that move inter-states/inter-localities. It is concluded that the disease is endemic in Kassala State, high prevalent in sheep and goats, posing a threat to animal exportation, and may have a serious economic influence. Owners and herders should compulsorily vaccinate their animals yearly and animals should be investigated periodically for implementation of crucial eradication program.
Cite this paper: Saeed, F. , Abdel-Aziz, S. and Gumaa, M. (2018) Seroprevalence and Associated Risk Factors of Peste des Petits Ruminants among Sheep and Goats in Kassala State, Sudan. Open Journal of Animal Sciences, 8, 381-395. doi: 10.4236/ojas.2018.84029.

[1]   Karrar, A.B. and Adam, E.A. (2006) Brief Overview of Sudan Economy and Future Prospects for Agricultural Development. Khartoum Food Aid Forum, Khartoum, 6-8 June 2006, 1-8.

[2]   Ministry of Animal Resources and Fisheries (2008) Department of Statistic and Information. Annual Report, Khartoum.

[3]   El Dirani, O.H., Jabbar, M.A. and Babiker, I.B. (2009) Constraint in Market Chains for Export of Sudanese Sheep and Sheep Meat to the Middle East. 16th Research Report, ILRI Publication Unit, Addis Ababa, 8-40.

[4]   Fadlalla, B. and Ahmed, F.A. (2010) Sudan Country Paper.

[5]   Office International des Epizooties (OIE) (2012) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter 2.7.11: Peste des Petits Ruminants.

[6]   Kinne, J., Kreutzer, R., Kreutzer, M., Wernery, U. and Wohlsein, P. (2010) Peste des Petits Ruminants in Arabian Wildlife. Epidemiology & Infection, 138, 1211-1214.

[7]   Ashley, C.B., Satya, P., Carrie, B., Chris, O., Oliver, K. and Libeau, G. (2010) Global Distribution of Peste des Petits Ruminant Virus Andprospects for Improved Diagnosis and Control. Journal of General Virology, 91, 2885-2897.

[8]   Murphy, F.A., Gibbs, E.P., Horzinek, M.C. and Studdert, M.J. (1999) Paramyxoviridae. In: Veterinary Virology, 3rd Edition, Academic Press, Cambridge, 411-428.

[9]   Roeder, P. and Obi, T.U. (1999) Recognizing of Peste des Petits Ruminants. In: FAO Animal Health Manual, Food and Agriculture Organization of the United Nations, Rome, 28.

[10]   Gargadennece, L. and Lalanne, A. (1942) La Peste des Petits Ruminants. Bulletin des Services Zootechnique et des Epizootie de l’Afrique Occidentale Francaise, 5, 16-21.

[11]   Braide, V.B. (1981) Peste des Petits Ruminants. A Review. World Animal Review, 39, 25-28.

[12]   Khalafalla, A.I., Saeed, I.K., Ali, Y.H., Abdurrahman, M.B., Kwiatek, O., Libeauc, G., Obeida, A.A. and Abbas, Z. (2010) An Outbreak of Peste des Petits ruminants (PPR) in the Sudan. Acta Tropica, 116, 161-165.

[13]   Murali, M., Muhammad, M., Aravindh, B., Parthiban, R., Ashley, C.B., Jingyue, B., Zhiliang, W., Chrisostom, A., Gelagay, A., Mehdi, H., Mana, M., Libeau, G., Carrie, B. and Satya, P. (2014) Molecular Evolution of Peste des Petits Ruminants Virus. Emerging Infectious Diseases, 20, 2023-2033.

[14]   Elhag Ali, B. (1973) A Natural Outbreak of Rinderpest Involving Sheep, Goats and Cattle in Sudan. Bulletin of Epizootic Diseases of Africa, 21, 421-428.

[15]   El Hag Ali, B. and Taylor, W.P. (1984) Isolation of Peste des Petits Ruminants Virus from Sudan. Research in Veterinary Science, 36, 1-4.

[16]   Rasheed, I.E. (1992) Isolation From Darfur State. Unpublished M.Sc. Thesis, University of Khartoum, Sudan.

[17]   Hassan, A.K., Ali, Y.O., Hajir, B.S., Fayza, A.O. and Hadia, J.A. (1994) Observation on Epidemiology of peste des petits Ruminant in Sudan. Sudan Journal of Veterinary Research, 13, 29-34.

[18]   Zeidan, M. (1994) Diagnosis and Distribution of PPR in Small Ruminants in Khartoum State during 1992-1994. Unpublished M.Sc. Thesis, University of Khartoum, Sudan.

[19]   El Amin, M.A.G. and Hassan, A.M. (1999) Rinderpest and PPR Surveillance in Sudan (1996-1998). Proceedings of a Research Co-Ordination Meeting, Machakos, 26-30 April 1999.

[20]   Salih, H.A.M.E., Elfadil, A.A.M., Saeed, I.K. and Ali, Y.H. (2014) Seroprevalence and Risk Factors of Pestedes Petits Ruminants in Sheep and Goats in Sudan. Journal of Advanced Veterinary Animal Research, 1, 42-49.

[21]   Enan, K.A., Intisar, K.S., Haj, M.A., Hussien, M.O., Tah, K.M., Elfahal, A.M., Ali, Y.H. and El Hussein, A.M. (2013) Seroprevalence of Two Important Viral Diseases in Small Ruminants in Marawi Province Northern State Sudan. International Journal of Livestock Production, 4, 18-21.

[22]   Ali, Y.H., Intisar, K.S. and Khalafalla, A.I. (2014) Outbreaks of Peste des petits Ruminants in Two Different Localities in Sudan. Journal of Veterinary Medicine and Animal Health, 6, 174-177.

[23]   Anon (2013-2015) Annual Reports of Kassala Veterinary Research Laboratory and Annual Reports of Department of Statistic, Ministry of Animal Resources and Fisheries, Kassala State.

[24]   Martin, S.W., Meek, A.H. and Willeberg, P. (1987) Veterinary Epidemiology: Principles and Methods. Iowa State University Press, Ames, 45.

[25]   Rogan and Gladen (1978) Estimating Prevalence from the Results of a Screening Test. American Journal of Epidemiology, 107, 71-76.

[26]   Shuaib, Y.A., El-Fadil, A.A.M., Abdel-Rahman, M.E., Negussie, H. and Zessin, K.H. (2014) Seroprevalence and Risk Factors of Peste des Petits Ruminants in Sheep in Kassala and North Kordofan States of the Sudan. International Journal of Veterinary Science, 3, 18-28.

[27]   Eldiem Taha Mostafa, S.A. and Elfadil, A.A.M. (2012) Sero-Prevalence and Risk Factors of Pesre des Petitss Ruminants (PPR) in Sheep in River Nile and White Nile States, Sudan. Journal of Veterinary Medicine and Animal Production, 3, 106-126.

[28]   Ozkul, A., Akca, Y., Alkan, F., Barrett, T., Karaoglu, T., Dagalp, S.B., Anderson, J., Yesilbag, K., Cokcaliskan, C., Gencay, A. and Burgu, I. (2002) Prevalence, Distribution, and Host Range of Peste des Petits Ruminants Virus, Turkey. Emerging Infectious Diseases Journal, 8, 709-712.

[29]   Arshadc, J. and Hussaind, M. (2008) Prevalence and Distribution of Peste des Petitsruminants Virus Infection in Small Ruminants. Small Ruminant Research, 79, 152-157.

[30]   Muse, E.A., Karimuribo, E.D., Gitao, G.C., Misinzo, G., Mellan, S.B., Msoffe, P.L.M., Swai, E.S. and Albano, M.O. (2012) Epidemiological Investigation into the Introduction and Factors for the Spread of PPR, Southern Tanzania, Onderstepoort. Journal of Veterinary Research, 79, 457-462.

[31]   Sarker, S. and Islam, H. (2011) Prevalence and Risk Factors of Peste des Petits Ruminants in Goats in Rajshai, Bangladesh. Veterinary World, 4, 546-549.

[32]   Kardjadja, M., Kouidria, B., Metrefa, D., Lukac, P.D. and Ben-Mahdib, M.H. (2015) Seroprevalence, Distribution and Risk Factor for Peste des Petits Ruminants (PPR) in Algeria. Preventive Veterinary Medicine, 122, 205-210.