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 JWARP  Vol.10 No.9 , September 2018
Enzyme Aided Low Temperature Evaporation for Concentration of Active Proteins from Potato Fruit Juice
Abstract: Extraction of starch from potato leads to formation of potato fruit juice (PFJ), which consists of proteins, fibers, starch and water. PFJ contains 1% - 3% [w/w] of proteins, including protease inhibitors that are potentially valuable for various applications, and could thus bring added value to the potato industry. The use of proteins of PFJ in bioactive form is limited by lack of benign and cost-efficient concentration technologies. The present approach combines a previously introduced low-temperature mechanical vapor compression evaporation technology with option to enzymatic viscosity management in case of high-viscosity fluids. In pilot-scale evaporation, an increase of solid content from 10% to 40% was achieved without major technical challenges. The proposed method offers a low-energy means for the concentration potato industry wastewater and reclamation of valuable proteins in active form.
Cite this paper: Ahokas, M. , Taskila, S. and Tanskanen, J. (2018) Enzyme Aided Low Temperature Evaporation for Concentration of Active Proteins from Potato Fruit Juice. Journal of Water Resource and Protection, 10, 846-856. doi: 10.4236/jwarp.2018.109048.
References

[1]   Alt, V., Steinhof, R., Lotz, M., Ulber, R., Kasper, C. and Scheper, T. (2005) Optimization of Glycoalkaloid Analysis for Use in Industrial Potato Fruit Juice Down Streaming. Engineering in Life Sciences, 5, 562-567.
https://doi.org/10.1002/elsc.200520107

[2]   Ralet, M.C. and Gueguen, J. (1999) Potato Proteins: Composition, Recovery and Functional Properties. Sciences des Aliments, 19, 147-165.

[3]   Ahokas, M., Välimaa, A.L., Lötjönen, T., Kankaala, A., Taskila, S. and Virtanen, E. (2014) Resource Assessment for Potato Biorefinery: Side Stream Potential in Northern Ostrobothnia. Agronomy Research, 12, 695-704.

[4]   Schieber, A. and Saldana, M.A. (2009) Potato Peels: A Source of Nutritionally and Pharmacologically Interesting Compounds—A Review. Food, 3, 23-29.

[5]   Bergthaller, W., Witt, W. and Goldau, H. (1999) Potato Starch Technology. Starch- Stärke, 51, 235-242.
https://doi.org/10.1002/(SICI)1521-379X(199907)51:7<235::AID-STAR235>3.0.CO;2-7

[6]   Straetkvern, K.O. and Schwarz, J.G. (2012) Recovery of Native Potato Protein Comparing Expanded Bed Adsorption and Ultrafiltration. Food and Bioprocess Technology, 5, 1939-1949.
https://doi.org/10.1007/s11947-010-0494-2

[7]   Schoenbeck, I., Graf, A.M., Leuthold, M., Pastor, A., Beutel, S. and Scheper, T. (2013) Purification of High Value Proteins from Particle Containing Potato Fruit Juice via Direct Capture Membrane Adsorption Chromatography. Journal of Biotechnology, 168, 693-700.
https://doi.org/10.1016/j.jbiotec.2013.09.018

[8]   Taskila, S., Ahokas, M., Järvinen, J., Toivanen, J. and Tanskanen, J.P. (2017) Concentration and Separation of Active Proteins from Potato Industry Waste Based on Low-Temperature Evaporation and Ethanol Precipitation. Scientifica.

[9]   Schmidt, J.M., Greve-Poulsen, M., Damgaard, H., Hammershöj, M. and Larsen, L.B. (2016) Effect of Membrane Material on the Separation of Proteins and Polyphenol Oxidase in Ultrafiltration of Potato Fruit Juice. Food and Bioprocess Technology, 9, 822-829.
https://doi.org/10.1007/s11947-015-1670-1

[10]   Dabestani, S., Arcot, J. and Chen, V. (2017) Protein Recovery from Potato Processing Water: Pre-Treatment and Membrane Fouling Minimization. Journal of Food Engineering, 195, 85-96.
https://doi.org/10.1016/j.jfoodeng.2016.09.013

[11]   Srichuwong, S., Fujiwara, M., Wang, X., Seyama, T., Shiroma, R., Arakane, M., et al. (2009) Simultaneous Saccharification and Fermentation (SSF) of Very High Gravity (VHG) Potato Mash for the Production of Ethanol. Biomass and Bioenergy, 33, 890-898.
https://doi.org/10.1016/j.biombioe.2009.01.012

[12]   Ribatski, G. and Jacobi, A.M. (2005) Falling-Film Evaporation on Horizontal Tubesa—A Critical Review. International Journal of Refrigeration, 28, 635-653.
https://doi.org/10.1016/j.ijrefrig.2004.12.002

[13]   Iida, Y., Tuziuti, T., Yasui, K., Towata, A. and Kozuka, T. (2008) Control of Viscosity in Starch and Polysaccharide Solutions with Ultrasound after Gelatinization. Innovative Food Science & Emerging Technologies, 9, 140-146.
https://doi.org/10.1016/j.ifset.2007.03.029

[14]   Braddock, R.J. and Kesterson, J.W. (1976) Enzyme Use to Reduce Viscosity and Increase Recovery of Soluble Solids from Citrus Pulp-Washing Operations. Journal of Food Science, 41, 82-85.
https://doi.org/10.1111/j.1365-2621.1976.tb01106.x

[15]   Poonsrisawat, A., Wanlapatit, S., Paemanee, A., Eurwilaichitr, L., Piyachomkwan, K. and Champreda, V. (2014) Viscosity Reduction of Cassava for Very High Gravity Ethanol Fermentation Using Cell Wall Degrading Enzymes from Aspergillus aculeatus. Process Biochemistry, 49, 1950-1957.
https://doi.org/10.1016/j.procbio.2014.07.016

[16]   Zhang, L., Zhao, H., Gan, M., Jin, Y., Gao, X., Chen, Q., et al. (2011) Application of Simultaneous Saccharification and Fermentation (SSF) from Viscosity Reducing of Raw Sweet Potato for Bioethanol Production at Laboratory, Pilot and Industrial Scales. Bioresource Technology, 102, 4573-4579.
https://doi.org/10.1016/j.biortech.2010.12.115

[17]   Eriksson, L., Johansson, E., Kettaneh-Wold, N., Wikström, C. and Wold, S. (2000) Design of Experiments. Principles and Applications, Learn Ways AB, Stockholm.

[18]   Kangas, A., Laine, A, Niskanen, M., Salo, Y., Vuorinen, M., Jauhiainen, L., et al. (2008) Results of Official Variety Trials 2001-2008. ISBN 978-952-487-210-2. MTT Agrifood Research Finland.

[19]   Ralet, M.C. and Gueguen, J. (2000) Fractionation of Potato Proteins: Solubility, Thermal Coagulation and Emulsifying Properties. Lebensmittel-Wissenschaft Und- Technologie-Food Science and Technology, 33, 380-387.
https://doi.org/10.1006/fstl.2000.0672

 
 
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