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 Health  Vol.10 No.9 , September 2018
Effect of 528 Hz Music on the Endocrine System and Autonomic Nervous System
Abstract: This study examined the stress-reducing effect on the endocrine system and the autonomic nervous system of music with a frequency of 528 Hz, which has recently attracted attention as a “healing” type of music. Nine healthy participants (one man and eight women, aged 26 - 37 years) listened to 528 Hz and standard 440 Hz music on separate days. We measured salivary biomarkers of stress (cortisol, chromogranin A, and oxytocin) before and after exposure to music, and continuously recorded the activity of the autonomic nervous system. The Profile of Mood State, 2nd edition, was also administered as a subjective indicator of stress. In the 528 Hz condition, mean levels of cortisol significantly decreased, chromogranin A tended to decrease, and oxytocin significantly increased after music exposure. However, no significant change was observed in any salivary biomarkers in the 440 Hz condition. The ratio of low frequency to high frequency autonomic nervous system activity significantly decreased after exposure to both types of music, and the coefficient of variation of R-R intervals also significantly decreased, but only after exposure to 528 Hz music. Tension-anxiety and Total Mood Disturbance scores were significantly reduced after exposure to 528 Hz music, while there was no significant difference following 440 Hz music. These results suggest that the influence of music on the autonomic nervous system and endocrine system varies depending on the frequency of the music, and furthermore, that 528 Hz music has an especially strong stress-reducing effect, even following only five minutes of exposure.
Cite this paper: Akimoto, K. , Hu, A. , Yamaguchi, T. and Kobayashi, H. (2018) Effect of 528 Hz Music on the Endocrine System and Autonomic Nervous System. Health, 10, 1159-1170. doi: 10.4236/health.2018.109088.
References

[1]   Kai, M., Ichikawa, S., Nimi, M. and Iwanaga, M. (2006) The Effects of Acoustic Aspects of Music to Musical Impression. Hiroshima Daigaku Daigakuin Sogo Kagaku Kenkyuka Kiyo. Ningen Kagaku Kenkyu, 1, 27-37. (In Japanese)

[2]   Akiyama, K. and Sutoo, D. (2011) Effect of Different Frequencies of Music on Blood Pressure Regulation in Spontaneously Hypertensive Rats. Neuroscience Letters, 487, 58-60.
https://doi.org/10.1016/j.neulet.2010.09.073

[3]   Nakajima, Y., Tanaka, N., Mima, T. and Izumi, S.I. (2016) Stress Recovery Effects of High- and Low-Frequency Amplified Music on Heart Rate Variability. Behavioural Neurology, 2016, Article ID: 5965894.
https://doi.org/10.1155/2016/5965894

[4]   Miluk-Kolasa, B., Obminski, Z., Stupnicki, R. and Golec, L. (1994) Effects of Music Treatment on Salivary Cortisol in Patients Exposed to Pre-Surgical Stress. Experimental and Clinical Endocrinology & Diabetes, 102, 118-120.
https://doi.org/10.1055/s-0029-1211273

[5]   Khalfa, S., Bella, S.D., Roy, M., Peretz, I. and Lupien, S.J. (2003) Effects of Relaxing Music on Salivary Cortisol Level After Psychological Stress. Annals of the New York Academy of Sciences, 999, 374-376.
https://doi.org/10.1196/annals.1284.045

[6]   Saruta, J., Tsukinoki, K., Sasaguri, K., Ishii, H., Yasuda, M., Osamura, Y.R., et al. (2005) Expression and Localization of Chromogranin A Gene and Protein in Human Submandibular Gland. Cells Tissues Organs, 180, 237-244.
https://doi.org/10.1159/000088939

[7]   Nakane, H. (2008) Systemic Diseases Seen from Dentistry Stress Measurement Evaluation by Salivary Chromogranin A. Journal of Clinical Laboratory Medicine, 52, 451-454. (In Japanese)

[8]   Nakayama, H., Kanehira, T., Kashiwazaki, H., Matsushita, T., Yamaguchi, T. and Takehara, J. (2010) A Study on Biological Effects of Music Listening: Using Salivary Stress Markers as Indicators. Japanese Journal of Music Therapy, 10, 210-216. (In Japanese)

[9]   Nishimura, A., Ohira, T. and Iwai, M. (2003) Effects of Appreciating Music on Salivary Cortisol and Chromogranin A. Japanese Journal of Music Therapy, 3, 150-156. (In Japanese)

[10]   Ooishi, Y., Mukai, H., Watanabe, K., Kawato, S. and Kashino, M. (2017) Increase in Salivary Oxytocin and Decrease in Salivary Cortisol after Listening to Relaxing Slow-Tempo and Exciting Fast-Tempo Music. PLoS One, 12, e0189075.
https://doi.org/10.1371/journal.pone.0189075

[11]   Ohisa, N., Yoshida, K., Yanabe, T. and Kaku, M. (2006) Effect of Autonomic Nervous System Activity While Listening to Music. Autonomic Neuroscience: Basic and Clinical, 130, 63.
https://doi.org/10.1016/j.autneu.2006.08.016

[12]   Smith, J.L. and Noon, J. (1998) Objective Measurement of Mood Change Induced by Contemporary Music. Journal of Psychiatric and Mental Health Nursing, 5, 403-408.
https://doi.org/10.1046/j.1365-2850.1998.00148.x

[13]   Otsuji, M. and Sato, N. (2017) Relationship between Listeners’ Mental Health and Music Preference. Japanese Journal of Psychosomatic Medicin, 57, 160-172. (In Japanese)

[14]   Den, R., Toda, M., Nagasawa, S., Kitamura, K. and Morimoto, K. (2007) Circadian Rhythm of Human Salivary Chromogranin A. Biomedical Research, 28, 57-60.
https://doi.org/10.2220/biomedres.28.57

[15]   Morimoto, K., Toda, M. and Isshiki, Y. (2004) Evaluation of Endocrinological Stress Reactions Utilizing Sensitive Salivary Stress Markers Cortisol and Chromogranin A. Job Stress Research, 11, 205-209. (In Japanese)

[16]   Heuchert, J.P. and McNair, D.M. (2015) POMS 2 Japanese Version Manual. Kanekoshobo Ltd., Tokyo.

[17]   Izawa, S., Shirotsuki, K., Sugaya, N., Ogawa, N., Suzuki, K. and Nomura, S. (2007) The Application of Saliva to an Assessment of Stress: Procedures for Collecting and Analyzing Saliva and Characteristics of Salivary Substances. Japanese Journal of Complementary and Alternative Medicine, 4, 91-101. (In Japanese)
https://doi.org/10.1625/jcam.4.91

[18]   Kodama, T., Abe, T., Kanehira, T., Morita, M. and Funahashi, F. (2010) Analysis of Fluctuations of Stress Markers in Saliva. Hokkaido Journal of Dental Science, 31, 52-61. (In Japanese)

[19]   Takahashi, T. (2016) The Gut-Brain Axis from Various Mediators and Intestinal Flora to Food Functionality (Part 2) A Physiologically Active Substance Related to the Gut-Brain Axis Oxytocin. The Japanese Journal of Clinical Nutrition, 128, 759-765. (In Japanese)

[20]   Uvnäs-Moberg, K. (1998) Oxytocin May Mediate the Benefits of Positive Social Interaction and Emotions. Psychoneuroendocrinology, 23, 819-835.
https://doi.org/10.1016/S0306-4530(98)00056-0

[21]   Kudo, Y. (2013) Neuroscience. Yodosha Company, Tokyo.

[22]   Yuasa, S. (2009) Emotion Emotional Neural Circuit Functional Consideration Centering on the Amygdala. Brain Medical, 21, 321-328. (In Japanese)

[23]   Inoue, M. and Ikeda, M. (2014) The Trend of Clinical Study Using Salivary Chromogranin A. Journal of Kochi Women’s University Academy of Nursing, 40, 24-30. (In Japanese)

[24]   Harada, T., Ishizaki, F., Hamada, M., Horie, N., Nitta, Y., Nitta, K., et al. (2009) Circadian Rhythm of Heart-Rate Variability and Autonomic Cardiovascular Regulation in Parkinson’s Disease. The Autonomic Nervous System, 46, 333-340.

[25]   Ito, S., Harada, T., Yamamoto, R., Niyada, K., Ishizaki, F. and Miyazaki, Y. (2017) Effects of High-Resolution Music Box Sound via Headphones on Autonomic Functions. The Autonomic Nervous System, 54, 130-136. (In Japanese)

[26]   Kageyama, S., Mochio, S., Taniguich, I. and Abe, M. (1981) A Proposal of a Quantitative Autonomic Function Test. Jikeikai Medical Journal, 28, 81-85.

[27]   Shoda, H. and Adachi, M. (2012) Effects of the Listening Context on the Audience’s Heart-Rate Variability in the Piano Performance. Ongaku Shinrigaku Ongaku Ryoho Kenkyu Nenpo, 41, 148. (In Japanese)

 
 
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