AiM  Vol.8 No.7 , July 2018
The Effect of the Hot Water Extracts of the Paecilomyces hepiali and Cordyceps militaris Mycelia on the Growth of Gastrointestinal Bacteria
Abstract: The gastrointestinal health is very important aspect concerning human health. It refers to nutrient and vitamin absorption, food digestion and various infectious diseases. The Paecilomyces hepiali and Cordyceps militaris are highly enriched with cordycepin and ergosterol which are considered as anti bacterial substances. Present study finds out comparative effect of hot water extract of particular fungal material on growth of six species of gastrointestinal bacteria that belong to both aerobic and anaerobic and, consist with harmful and commensal categories. The appropriate concentration level of hot water extract of both strains was identified. The individual specific bacterial growing media were prepared and calculated; amounts of bacteria cultures were inoculated by using micro pipettes. The optical density and number of bacterial colonies were measured after 24 hours. The pure mycelial extract of P. hepiali with 2 × 10-3 g/ml of concentration has significant effect on depleting the growth of E. coli, E. faecalis, S. aureus, L. gasseri and B. ovatus bacteria. B. longum has no significant effect by particular extract. Same type of extract of C. militaris has significantly reduced the growth of every bacteria used in this study. Hot water extract of C. militaris cultivated on soy bean has significant growth retardation toward E. coli, E. faecalis, S. aureus and L. gasseri. It has stimulated the growth of B. ovatus and B. longum which are considered as beneficial bacteria for human gut. This study shows that extracts of both mycelia include antimicrobial substances like cordycepin and ergosterol which can be used as food supplements to enhance human gut health.
Cite this paper: Gamage, S. , Nakayama, J. , Fuyuno, Y. and Ohga, S. (2018) The Effect of the Hot Water Extracts of the Paecilomyces hepiali and Cordyceps militaris Mycelia on the Growth of Gastrointestinal Bacteria. Advances in Microbiology, 8, 490-505. doi: 10.4236/aim.2018.87034.

[1]   Liu, Y., Wang, J., Wang, W., Zhang, H., Zhang, X. and Han, C. (2015) The Chemical Constituents and Pharmacological Actions of Cordyceps sinensis. Evidence-Based Complementary and Alternative Medicine, 2015, Article ID 575063.

[2]   Chioza, A. and Ohga, S. (2014) A Review on Fungal Isolates Reported as Anamorphs of Ophiocordyceps sinensis. Journal of Mycology, 2014, Article ID 913917.

[3]   Zhang, G., Huang, Y., Bian, Y., Wong, J.H., Ng, T.B. and Wang, H. (2006) Hypoglycemic Activity of the Fungi Cordyceps militaris, Cordyceps sinensis, Tricholoma mongolicum, and Omphalia lapidescens in Streptozotocin-Induced Diabetic Rats. Applied Microbiology and Biotechnology, 72, 1152-1156.

[4]   Zhang, W., Yang, J., Chen, J., Hou, Y. and Han, X. (2004) Immunomodulatory and Antitumor Effects of Exopolysaccharide Fraction (EPSF) from a Cultivated Cordyceps sinensis Fungus on Tumor-Bearing Mice. Biotechnology and Applied Biochemistry, 42, 9-15.

[5]   Lo, H.C., Tu, S.T., Lin, K.C. and Lin, S.C. (2004) The Anti-Hyperglycemic Activity of the Fruiting Body of Cordyceps in Diabetic Rats Induced by Nicotinamide and Streptozotocin. Life Sciences, 74, 2897-2908.

[6]   Choi, S.B., Park, C.H., Choi, M.K., Jun, D.W. and Park, S. (2004) Improvement of Insulin Resistance and Insulin Secretion by Water Extracts of Cordyceps militaris, Phellinus linteus, and Paecilomyces tenuipes in 90% Pancreatectomized Rats. Bioscience, Biotechnology, and Biochemistry, 68, 2257-2264.

[7]   Yue, K., Ye, M., Zhou, Z., Sun, W. and Lin, X. (2013) The Genus Cordyceps: A Chemical and Pharmacological Review. Journal of Pharmacy and Pharmacology, 65, 474-493.

[8]   Tuli, H.S., Sharma, A.K., Sandhu, S.S. and Kashyap, D. (2013) Cordycepin: A Bioactive Metabolite with Therapeutic Potential. Life Sciences, 93, 863-869.

[9]   Ikeda, R., Nishimura, M., Sun, Y., Wada, M. and Nakashima, K. (2008) Simple HPLC-UV Determination of Nucleosides and Its Application to the Authentication of Cordyceps and Its Allies. Biomedical Chromatography, 22, 630-636.

[10]   Guan, J., Zhao, J., Feng, K., Hu, D.J. and Li, S.P. (2011) Comparison and Characterization of Polysaccharides from Natural and Cultured Cordyceps Using Saccharide Mapping. Analytical and Bioanalytical Chemistry, 399, 3465-3474.

[11]   Hooper, L.V., Wong, M.H., Thelin, A., Hansson, L., Falk, P.G. and Gordon, J.I. (2001) Molecular Analysis of Commensal Host-Microbial Relationships in the Intestine. Science, 291, 881-884.

[12]   Zhang, Y.J., Li, S., Gan, R.Y., Zhou, T., Xu, D.P. and Li, H.B. (2015) Impacts of Gut Bacteria on Human Health and Diseases. International Journal of Molecular Sciences, 16, 7493-7519.

[13]   Ahn, Y.J., Park, S.J., Lee, S.G., Shin, S.C. and Choi, D.H. (2000) Cordycepin: Selective Growth Inhibitor Derived from Liquid Culture of Cordyceps militaris against Clostridium spp. Journal of Agricultural and Food Chemistry, 48, 2744-2748.

[14]   Yang, L. (2008) Biorelevant Dissolution Testing of Colon-Specific Delivery Systems Activated by Colonic Microflora. Review. Journal of Controlled Release, 125, 77-86.

[15]   Mitsuoka, T. (1992) The Human Gastrointestinal Tract. In: Wood, B.J.B., Eds., The Lactic Acid Bacteria, Vol. 1, Springer, Boston, M.

[16]   Savage, D.C. (1986) Gastrointestinal Microflora in Mammalian Nutrition. Annual Review of Nutrition, 6, 155-178.

[17]   Guarner, F. and Malagelada, J.R. (2003) Gut Flora in Health and Disease. The Lancet, 361, 512-519.

[18]   Walker, D.K. and Gilliland, S.E. (1993) Relationships among Bile Tolerance, Bile Salt Deconjugation, and Assimilation of Cholesterol by Lactobacillus acidophilus. Journal of Dairy Science, 76, 956-961.

[19]   Wagner, R., Mitchell, D.A., Sassaki, G.L. and Amazonas, M.A.L.A. (2004) Links between Morphology and Physiology of Ganodrma lucidum in Submerged Culture for the Production of Exopolysaccharide. Journal of Biotechnology, 114, 153-164.

[20]   Hsieh, C., Hsu, T.H. and Yang, F.C. (2005) Production of Polysaccharides of Ganoderma lucidum (CCRC36021) by Reusing Thin Stillage. Process Biochemistry, 40, 909-916.

[21]   Ng, T.B. and Wang, H.X. (2005) Pharmacological Actions of Cordyceps, a Prized Folk Medicine. The Journal of Pharmacy and Pharmacology, 57, 1509-1519.

[22]   Huang, Y.L., Leu, S.F., Liu, B.C., Sheu, C.C. and Huang, B.M. (2004) In Vivo Stimulatory Effect of Cordyceps sinensis Mycelium and Its Fractions on Reproductive Functions in Male Mouse. Life Sciences, 75, 1051-1062.

[23]   Liu, N., Chen, X.G., Park, H.J., Liu, C.G., Liu, C.S., Meng, X.H. and Yu, L.J. (2006) Effect of MW and Concentration of Chitosan on Antibacterial Activity of Escherichia coli. Carbohydrate Polymers, 64, 60-65.

[24]   Kim, H.O. and Yun, J.W. (2005) A Comparative Study on the Production of Exopolysaccharides between Two Entomopathogenic Fungi Cordyceps militaris and Cordyceps sinensis in Submerged Mycelial Cultures. Journal of Applied Microbiology, 99, 728-739.

[25]   Song, C.H., Jeon, Y.J., Yang, B.K., Ra, K.S. and Sung, J.M. (1998) The Anti-Complementary Activity of Exo-Polymers Produced from Submerged Mycelial Cultures of Higher Fungi with Particular Reference to Cordyceps militaris. Journal of Microbiology and Biotechnology, 8, 536-539.

[26]   Yang, F.C., Huang, H.C. and Yang, M.J. (2003) The Influence of Environmental Conditions on the Mycelial Growth of Antrodia cinnamomea in Submerged Cultures. Enzyme and Microbial Technology, 33, 395-402.

[27]   Hwang, H.J., Kim, S.W., Xu, C.P., Choi, J.W. and Yun, J.W. (2003) Production and Molecular Characteristics of Four Groups of Exopolysaccharides from Submerged Culture of Phellinus gilvus. Journal of Applied Microbiology, 94, 708-719.

[28]   Kim, S.W., Xu, C.P., Hwang, H.J., Choi, J.W., Kim, C.W. and Yun, J.W. (2003) Production and Characterization of Exopolysaccharides from an Entomopathogenic Fungus Cordyceps militaris NG3. Biotechnology Progress, 19, 428-435.

[29]   Xiao, J.H., Chen, D.X., Liu, J.W., Wan, W.H., Fang, N., Xiao, Y., Qi, Y. and Liang, Z.Q. (2004) Optimization of Submerged Culture Requirements for the Production of Mycelial Growth and Exopolysaccharide by Cordyceps jiangxiensis JXPJ 0109. Journal of Applied Microbiology, 96, 1105-1116.

[30]   Yang, F.Q., Li, D.Q., Feng, K., Hu, D.J. and Li, S.P. (2010) Determination of Nucleotides, Nucleosides and Their Transformation Products in Cordyceps by Ion-Pairing Reversed-Phase Liquid Chromatography-Mass Spectrometry. Journal of Chromatography A, 1217, 5501-5510.

[31]   Paterson, R.R.M. (2008) Cordyceps—A Traditional Chinese Medicine and Another Fungal Therapeutic Biofactory? Phytochemistry, 69, 1469-1495.

[32]   Xiao, J.H., Qi, Y. and Xiong, Q. (2013) Nucleosides, a Valuable Chemical Marker for Quality Control in Traditional Chinese Medicine Cordyceps. Recent Patents on Biotechnology, 7, 153-166.

[33]   Qian, G.M., Pan, G.F. and Guo, J.Y. (2012) Anti-Inflammatory and Antinociceptive Effects of Cordymin, a Peptide Purified from the Medicinal Mushroom Cordyceps sinensis. Natural Product Research, 26, 2358-2362.

[34]   Zhou, X., Luo, L., Dressel, W., Shadier, G., Krumbiegel, D., Schmidtke, P., Zepp, F. and Meyer, C.U. (2008) Cordycepin Is an Immunoregulatory Active Ingredient of Cordyceps sinensis. The American Journal of Chinese Medicine, 36, 967-980.

[35]   Li, Y.H. and Li, X.L. (1991) Determination of Ergosterol in Cordyceps sinensis and Cordyceps Black-Bone Chicken Capsules by HPLC. Acta Pharmaceutica Sinica, 26, 768-771.

[36]   Kitchawalit, S., Kanokmedhakul, K., Kanokmedhakul, S. and Soytong, K. (2014) A New Benzyl Ester and Ergosterol Derivatives from the Fungus Gymnoascus reessii. Natural Product Research, 28, 1045-1051.

[37]   Rajput, S.B. and Karuppayil, S.M. (2013) Small Molecules Inhibit Growth, Viability and Ergosterol Biosynthesis in Candida albicans. Springer Plus, 2, 26.

[38]   Yang, F.Q., Ge, L., Yong, J.W.H., Tan, S.N. and Li, S.P. (2009) Determination of Nucleosides and Nucleobases in Different Species of Cordyceps by Capillary Electrophoresis-Mass Spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 50, 307-314.

[39]   Vinderola, C.G. and Reinheimer, J.A. (2003) Lactic Acid Starter and Probiotic Bacteria: A Comparative ‘‘in Vitro’’ Study of Probiotic Characteristics and Biological Barrier Resistance. Food Research International, 36, 895-904.