AiM  Vol.8 No.7 , July 2018
Establishment of Real-Time Fluorescent PCR Method for Detection of D Epizootic Hemorrhagic Disease of Deer
Abstract: Epizootie hemorrhagic disease of deer virus (EHDV), is a member of the family Reoviridae virus (Orbi virus), and a double-stranded RNA virus from arthropods (mainly Culicoides), which is very similar in morphology and structure to Blue tongue virus (BTV). Serologically, there is a clear cross between the blue tongue virus and EHDV. EHD (caused by EHDV) is a seasonal, non-contact viral infectious disease. Under natural conditions, EHDV can cause infection of cattle, sheep and other domestic animals as well as many domesticated and wild ruminants such as white tail deer, Mi, large horned antelope and so on. The virus has been isolated from white-tailed deer, black-tailed deer, sheep, goats, cattle, bison and Culicoides. White tail deer infection is the most serious, and clinical characteristics are transient elevated body temperature, symptoms of shock, mucosal, serous bleeding and death in a coma [1]. At present, there is no effective vaccine and treatment for this disease. To control and eliminate EHD, it is necessary to spend a lot of manpower and material resources. Export trade is restricted, which brings great economic losses to the countries with EHD epidemic. Moreover, because of the complexity of antigen structure and the diversity of clinical symptoms of EHDV, it is difficult to diagnose in laboratory. Therefore, developing a quick and precise laboratory diagnostic method becomes more and more significant among researchers.
Keywords: Loop-Mediated
Cite this paper: Wu, B. , Wan, C. , Su, L. and Guan, P. (2018) Establishment of Real-Time Fluorescent PCR Method for Detection of D Epizootic Hemorrhagic Disease of Deer. Advances in Microbiology, 8, 476-481. doi: 10.4236/aim.2018.87032.

[1]   Ruan, Z.X., Yang, J.X., et al. (2010) Establishment of Multiple RT-PCR Detection Method for Four Animal Insect-Borne Viruses. Advances in Animal Medicine, No. 2, 25-31.

[2]   Jia, J.J. and Li, W.G. (2003) PCR Technique Was Used to Detect Deer Epidemic Hemorrhage Virus. Animal Husbandry and Veterinarian, 35, 24-26.

[3]   Chen, J.C., et al. (2010) Establishment of a Real-Time Fluorescent PCR Method for Rapid Detection of Deer Epidemic Hemorrhage Virus. Modern Journal of Integrated Chinese and Western Medicine, 19, 156-158.