Consistent feed consumption by the sow is critical to sustained health of the animal through the stressful time of gestation and lactation, as well as the health and growth of the piglets. A sow that maintains body weight during gestation/lactation will be in better health and more likely to achieve a healthy litter the next breeding cycle. Feeds are many times produced utilizing ingredients from different production facilities, different carriers, and/or the plant-derived ingredients are from different growing regions, any of which may alter the taste of the feed. A key aspect to commercial swine production is the consistent consumption of feed throughout the growth process. Changes in the organoleptic qualities of the feed may often occur, either as stated above or when feed composition is changed as the pig moves from one life stage (e.g., piglet) to another (e.g., growing/finishing), which decreases overall feed intake  . Piglets that were exposed to feed flavors prenatally through the maternal diet recognized the flavor during reexposure up to 23 days after birth, indicating that the addition of flavors or flavor modifiers to provide a consistent flavor profile can reduce inhibition of feed intake, resulting in less stress on the sow and on the piglets . Plant-based organoleptic feed ingredients, such as essential oils, herbs and other phytogenic substances are being added to swine feed to help maintain or increase feed palatability and overall health and growth of the swine . SangrovitÒ is the trade name of a feed ingredient containing a Macleaya cordata extract (MCE) within a carrier (MCE preparation; MCEP), standardized to contain a minimum concentration of 1.5% sanguinarine in the final product. SangrovitÒ provides a consistent flavor profile to feed, which helps maintain consistent feed intake over the life of the swine. Previous studies have evaluated the consumption of MCEP by growing and finishing swine   , with SangrovitÒ added to the diet at 50 ppm providing a positive effect on body weight gain and feed conversion rate in weanling and growing swine   ; however, little is known on the consumption of SangrovitÒ by sows during gestation and lactation. To that end, feeding trials have been conducted with SangrovitÒ consumed in the diet by sows during the late gestation phase through piglet weaning.
2. Material and Methods
2.1. Preliminary Field Trials
For future preparation of a more controlled trial, two preliminary field trials were conducted in Vietnam that evaluated the addition of SangrovitÒ to the diets of sows during gestation and lactation  . The first preliminary trial (Trial #1) was conducted under field conditions in which the sows (N = 9/group; breed not specified) were fed either the control diet (nutrient/ingredient data not retained), or the control diet supplemented with SangrovitÒ (100 ppm), starting two weeks prior to farrowing, until weaning at 28 days of age . The control diet during the gestation phase contained chlortetracycline (3000 ppm), Bacitracine-Methylene-Disalicylate (500 ppm), copper-sulfate (25%, 100 ppm), De-odoraseÒ1 (100 ppm) and a mycotoxin binder (specific binder not specified in the internal report; 1000 ppm). During the lactation phase, the control diet contained added amoxycilline (99%, 300 ppm), Bacitracine-Methylene-Disalicylate (500 ppm), copper-sulfate (25%, 100 ppm), De-odoraseÒ (100 ppm) and a mycotoxin binder (specific binder not specified in the internal report; 1000 ppm).
The second preliminary trial (Trial #2) was conducted under field conditions in which the sows (Yorkshire/Landrace × Duroc/Landrace cross breed; N = 10/group) were fed either a control diet or the control diet to which SangrovitÒ was added at 100 ppm feed . The control diet formulation is provided in Table 1. The sows were fed with either the control or SangrovitÒ―containing diet from four weeks prior to farrowing until weaning (average weaning age of the piglets: 25 days). Endpoints evaluated included: number of piglets/litter (total and live), piglet weight at birth and weaning, survival rate (%) and the daily rate of sick piglets (sickness parameters included evaluation of piglets for diarrhea, leg weakness and arthritis).
2.2. Confirmatory Field Trial
A confirmatory field trial (Trial #3) was conducted in Thailand using a least squares (LSD) design through two consecutive parities, utilizing 90 Yorkshire*Landrace sows (average initial body weight at 211 kg) . The experiment consisted of two phases: late gestation and lactation. The basal diet provided to the sows during these two periods is provided in Table 2.
During the late gestation phase, second- and third-parity sows (N = 30/group) were randomly allocated to three groups. Each block represented a similar initial farrowing date. The three groups were: 1) control basal diet, 2) control diet +
Table 1. Composition of basal diets, on an as fed basis (%) (Trial #2).
Table 2. Composition of basal diets, on an as fed basis (%) in Trial #3.
SangrovitÒ (50 ppm), and 3) control diet + SangrovitÒ (100 ppm). The sows were housed in a confinement gestation facility and fed individually in stalls. The individual gestation stalls (2.1 m × 0.5 m) had 0.9 m of concrete in the front and 1.2 m of slotted floor (i.e., 15.2 cm concrete slat and 2.5 cm slot) in the back of the stall. An average of 2.5 kg feed was provided to the sow daily. Body weight change and feed intake were recorded from Day 100 of gestation through farrowing (approximately Day 114).
At farrowing, the sows were placed in farrowing crates (2.1 m × 0.6 m) that had an area for the piglets (2.1 m × 0.5 m) on each side of the stall (lactation phase). The floor was woven wire and each crate contained an electric heat box (0.6 m × 0.3 m) in the pig area and a 0.6 m × 0.6 m rubber pad located below the sow shoulder area. The sows were kept to their original three groups, receiving their respective diets: 1) control, basal diet, 2) control diet + SangrovitÒ (50 ppm), and 3) control diet + SangrovitÒ (100 ppm). Sows were hand-fed the diet to appetite twice daily throughout the three-week lactation period. Changes in sow body weight and feed intake from farrowing to weaning at 21 days of piglet age were recorded. At 3 days of age, the litters were standardized to ten piglets/sow. Culled piglets were randomly selected and placed with another litter of similar age but not as part of the study. Within the experiment, the piglets remained in the same treatment groups defined by their dams. Piglet birth and weaning weights were recorded. Statistical analysis was conducted utilizing ANOVA procedure within SAS (1988)2, with treatment effects tested for linear and quadratic responses. Pseudo standard error was utilized to evaluate significance .
3. Results and Discussion
3.1. Preliminary Trials
Sows fed SangrovitÒ at 100 ppm from late gestation (two weeks before farrowing) through weaning of the resultant piglets (Day 28 post birth) in the first preliminary trial (Trial #1) resulted in a slightly increased feed intake by the sows (statistical analysis not conducted), which corresponded to increased piglet weights both at birth and at weaning (Table 3). The slight increase in sow feed intake may have resulted in increased milk production, which could have been the reason for the increased number of piglets reaching weaning age (Table 3).
The second preliminary trial (Trial #2) had an increased gestation period (four weeks feeding of control diet or control diet plus SangrovitÒ at 100 ppm), but a slightly decreased weaning age (average of 25 days post birth), compared to the previous trial. Study results focused on piglet growth, with the apparent assumption that increased piglet health and growth parameters was dependent on better health of the sows fed 100 ppm SangrovitÒ in the diet. The SangrovitÒ-fed group had a higher number of piglets/sow (4.7% greater than control), which was correlated with a decreased body weight/piglet (Table 4). However, the weight at weaning (3.8%) and overall weight gain (5.4%) was increased, relative to values found in the control group, and a decreased percentage of piglets during the trial period (Table 4).
3.2. Confirmatory Trial
In the confirmatory trial (Trial #3), no differences in sow reproductive performance were observed among the dietary treatments (Table 5). Average daily feed intake (ADFI) was slightly increased with increasing concentrations of SangrovitÒ, although the increases did not reach statistical significance (P > 0.05). Although there were no significant differences between treatment groups and the control group on sow body weight change from Day 0 through sow weight at weaning (P > 0.05), there was an observable inhibition of a decrease in bodyweight between dose groups, which was also reflected in an inhibition of decreasing change in sow back fat (Table 5). At the start of the study (Day 100 of gestation), the sow body condition score did not differ (P > 0.10). Overall, the
Table 3. Effect of SangrovitÒ consumption by sows on piglet growth (Trial #1).
*Percent relative to control.
Table 4. Piglet growth and survival during lactation on sows consuming Sangrovit diet (Trial #2).
*Percent relative to control.
Table 5. Effect of SangrovitÒ in lactation diets on sow performance (Trial #3).
a,bMeans within a row with different superscripts differ (P < 0.05); PSE = Pseudo Standard Error; analyzed as least square means.
body condition scores for the sows consuming diets containing 50 and 100 ppm SangrovitÒ were significantly (P < 0.05) greater than the control group, indicating that even a small increase in feed intake has a positive effect on the maintenance of the sow during late gestation and lactation phases of the life cycle.
The inclusion of SangrovitÒ in the sow diets had no effects on farrowed litter size or the litter size at weaning (Table 6), but the percent mortality prior to weaning was significantly (P < 0.05) decreased in the groups fed SangrovitÒ, compared to the control group. The increased consumption of feed by the sows had a positive effect on the growth of the piglets, resulting in significantly (P <
Table 6. Effect of SangrovitÒ in sow lactation diets on litter performance (Trial #3).
a,bMeans within a row with different superscripts tend to differ (P < 0.05); PSE = Pseudo Standard Error; analyzed as least square means.
0.05) increased litter weight gain and litter weight at weaning.
Evaluation of the piglet growth during the study found that sow consumption of SangrovitÒ had no effect on piglet growth seven days after birth, but by 14 days the addition of SangrovitÒ to the sow diet significantly (P < 0.05) increased piglet body weight (Table 7), and was maintained until the end of the study (i.e., weaning at Day 21).
Small increases in sow feed consumption can have significant effects on piglet growth, presumably through increased milk production, as the piglet weight gain from birth through Day 7 of life was significantly increased in the sow groups fed Sangrovit® at both 50 and 100 ppm, compared to the control group. The positive piglet weight gain was maintained through weaning (Table 8).
Field trials are able to provide an indication of potential effects of the addition of flavoring ingredients to swine feed. Trials were conducted on the addition of SangrovitÒ, a phytogenic feed ingredient on the growth and health of sows during late gestation and lactation and their piglets found slight increases in feed intake by the sows in the preliminary trials and corresponding increases in piglet body weights prior to weaning. The results from these field trials (e.g., decreased mortality, increased piglet body weight) indicate that consistent feed consumption by the sows also helped maintain the health of the piglets. In the confirmatory trial, even this slight increase in feed intake resulted in increased sow body condition score and an inhibition of the decrease in sow back fat, an indicator of sow health. Increasing concentration of SangrovitÒ in the diet decreased the loss of sow body weight during the study, while maintaining the growth and wellbeing of the piglets, as indicated by increased litter weight at weaning and overall litter weight gain. These field trials show that SangrovitÒ fed to sows during late gestation and during lactation help to maintain the health of the sows and the
Table 7. Piglet body weight from day at birth to weaning (Trial #3).
*Mean ± SD. abMeans within a row with different superscripts tend to differ (P < 0.05); **Day indicates day of age; #Day 21 was date of weaning.
Table 8. Piglet weight gain from birth - Day 7 of age and from Day 7 - Day 21 (weaning) (Trial #3).
*Mean ± SD. a,bMeans within a row with different superscripts tend to differ (P < 0.05); **Piglet mortality rate calculated from birth to Day 7.
resulting piglets. Future rigorous, placebo-controlled, randomized and blinded studies that are statistically evaluated would be helpful in confirming the results of these field trials.
1Limestone; 2KemzymeÒ = stabilized, multi-enzyme feed ingredient; https://kemin.com/eu/en/products/kemzyme
2SAS Institute Inc., Cary, NC U.S.A.
 Dong, G.Z. and Pluske, J.R. (2007) The Low Feed Intake in Newly-Weaned Pigs: Problems and Possible Solutions. Asian-Australasian Journal of Animal Sciences, 20, 440-452.
 Clouard, C. and Val-Laillet, D. (2014) Impact of Sensory Feed Additives on Feed Intake, Feed Preferences, and Growth of Female Piglets during the Early Postweaning Period. Journal of Animal Science, 92, 2133-2140.
 Oostindjer, M., Bolhuis, J.E., van den Brand, H. and Kemp, B. (2009) Prenatal Flavor Exposure Affects Flavor Recognition and Stress-Related Behavior of Piglets. Chemical Senses, 34, 775-787.
 Jacela, J.Y., DeRouchey, J.M., Tokach, M.D., Goodband, R.D., Nelssen, J.L., Renter, D.G. and Dritz, S.S. (2010) Feed Additives for Swine: Fact Sheets—Flavors and Mold Inhibitors, Mycotoxin Binders, and Antioxidants. Journal of Swine Health and Production, 18, 27-32.
 Zhao, L., Matulka, R.A., von Alvensleben, S. and Morlacchini, M. (2017) Residue Study for a Standardized Macleaya cordata Extract in Growing-Finishing Swine. Open Journal of Animal Sciences, 7, 93-104.
 Zhao, L., von Alvensleben, S., Fusconi, G. and Morlacchini, M. (2017) Safety Evaluation of a Standardized Macleaya cordata Extract in a Ninety Day Feeding Study in Weaned Piglets. Open Journal of Animal Sciences, 7, 213-231.
 Jeroch, H., Kozlowski, K., Jeroch, J., Lipinski, K., Zdunczyk, Z. and Jankowski, J. (2009) Efficacy of the Phytogenic (Papaveraceae) Additive Sangrovit in GrowingMonogastric Animals. Zuchtungskunde, 81, 279-293.
 Kantas, D., Papatsiros, V.G., Tassis, P.D., Athanasiou, L.V. and Tzika, E.D. (2015) The Effect of a Natural Feed Additive (Macleaya cordata), Containing Sanguinarine, on the Performance and Health Status of Weaning Pigs. Animal Science Journal, 86, 92-98.