Primary fallopian tube cancer is extremely rare, accounting for 0.14% - 1.8% of malignancies in women annually     . Although it is hard to differentiate histopathologically and clinically from ovarian cancer, the management of both is similar  . The etiology of this malignancy is unknown. However, fallopian tube cancer has been reported in high-risk breast-ovarian cancer families with germ-line mutations of BRCA-1 and BRCA-2  . The CA-125 antigen is often expressed by this malignancy, and serum level of CA-125 is elevated in >80% of patients with this disease pretreatment  .
Several case reports described usefulness of cervical or vaginal smears for the diagnosis of fallopian tube cancer. However, positive Papanicolaou (Pap)-stained smears have been reported in only 0% - 2.3% of cases  . The diagnosis of this malignancy is usually first made by a pathologist on histopathological examinations. Since it is difficult to differentiate fallopian tubal cancer from ovarian cancer, the patients with at least one of the following criteria  should be diagnosed as this malignancy: 1) the main tumor is in the tube and develops from the endosalpinx; 2) the pattern histopathologically reproduces the tubal epithelium and often shows a papillary pattern; 3) if the wall is involved, the transition between normal and malignant epithelium should be observed; and 4) the ovaries and endometrium are either normal or contain less tumor than the tube.
We report here a case of the right fallopian tube cancer, which was discovered by the vaginal smear during the close inspection of para-aortic adenopathy found when general searching metastasis of the right upper lobe lung cancer. Although the tubal cancer was very small and the patient had no significant clinical signs, we noticed its metastasis to the para-aortic lymph node.
2. Case Presentation
This case report presents a 47-year-old Japanese female housewife. She was nulliparous and had no previous known gynecological problems. Her past history included removal of thyroid tumor at 43 years old. In August, 2017, her medical checkup revealed lung cancer (well-differentiated bronchioloalveolar carcinoma) of the right upper lobe (Figure 1). A week later, positron emission tomography (PET)-computed tomography (CT) examination revealed abnormal 18-fluorodeoxyglucose (FDG) uptake in the para-aortic lymph node, suggesting lymph node metastasis of lung cancer. Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) examination of the lymph node confirmed poorly-differentiated adenocarcinoma on the Pap-stained cytologic (Figure 2(A)) and on the H & E-stained histopathologic specimens (Figure 2(B)). However, this metastatic lesion was histopathologically different from lung cancer. Therefore, we suspected the metastatic lesion was derived from malignancy in organ other than lung. One month later, her vaginal smear was positive for adenocarcinoma (Figure 3(A)), although the pelvic contrast magnetic resonance imaging (MRI) examination did not show any abnormalities in the uterine corpus, uterine cervix, and bilateral adnexa. Cervical Pap smear reported CIN1 with koilocytosis. In order to determine the primary site of the lymph node malignancy, we tried to diagnose endometrial cytology and histology, but we were not able to
Figure 1. Biopsy specimens of lung tumor in the right upper lobe show bronchioloalveolar carcinoma, so-called BAC. H & E-stain, bar = 50 μm.
Figure 2. EUS-FNA specimens from para-aortic lymph node shows papillar adenocarcinoma on (A) the Papanicolaou-stained smears and (B) H & E-stained histological section. Bars = (A) 20 μm and (B) 50 μm.
Figure 3. The 1st vaginal smear shows papillary adenocarcinoma (A). Papillary clusters of atypical glandular cells with increased nucleo-cytoplasmic ratio, hyperchromatic nuclei, and large nucleoli. On the 2nd vaginal smear (B), only one papillary cluster consisted of atypical glandular cells with enlarged nuclei and nucleoli, suggesting adenocarcinoma. (A) and (B), Papanicolaou stain, bars = 20 μm.
obtain samples because cervical duct was closed down. Serum levels of tumor markers, including CA-125 (10.7 U/ml, reference value: ~35 U/ml), CA19-9 (<2.0 U/ml, reference value: ~37 U/ml), SCC (0.6 ng/ml, reference value: ~1.5 ng/ml)were normal range. Re-examination of vaginal smear on October 11, 2017 was also positive for adenocarcinoma (Figure 3(B)), while cervical curettage showed no abnormality. Gastrointestinal tract studies by upper and lower endoscopy showed normal. Although primary site of the malignancy was unknown, we suspected gynecological malignancy, possibly fallopian tube cancer. We decided then to perform abdominal simple hysterectomy, bilateral appendages extirpation and removal of para-aortic lymph nodes in November, 2017. During the operation, peritoneal lavage cytology was positive for adenocarcinoma (Figure 4), but the peritoneum was macroscopically normal. While the uterus, left ovary, the left fallopian tube, and the right ovary showed no abnormalities (Figure 5(A)), the right fallopian tube (Figure 5(A), circled) was slightly enlarged and nodular. The nodular lesions were consisted of diffusely proliferated tumor cells with small and large round nuclei and prominent nucleoli, suggesting poorly differentiated adenocarcinoma (Figure 5(B)). Immunohistochemistry revealed that cancer cells were positive against AE1/AE3, CK7, and ER, while they were negative for CK20, CEA, and calretinin. Most cancer cells showed positive reaction against p53 (Figure 5(C)). A few tubal epithelial cells also had nuclei slightly positive for p53. Similar cancer cells were detected in the para-aortic lymph node. Our final diagnosis was right fallopian tube adenocarcinoma (poorly differentiated) with para-aortic lymph node metastasis (Stage IIIc: pT1cN1M0). Four months later, the upper lobectomy (Figure 6(A)) of the right lung was performed and the lung cancer was histopathogically well-differentiated adenocarcinoma (Stage IB, T2aN0M0) (Figure 6(B)). She received four courses of systemic chemotherapy with paclitaxel (175 mg/m2) and carboplatin (AUC: 6 mg/ml/min every 21 days) combined with bevacizumab (15 mg/kg).
The patient is doing fine at 9-months follow-up with no evidence of reccurrence/distant metastasis of two malignancies after surgery and chemotherapy. We should intensively follow-up, since the actuarial 5-year survival rate was 38% - 50%   .
Figure 4. Peritoneal lavage cytology was suggestive of adenocarcinoma. The sheet of atypical glandular cells with large nucleoli is present. Papanicolaou stain, bar = 20 μm.
Figure 5. (A) Macroscopic view of the resected uterus and bilateral appendages. Note: the right tube (circled) was slightly enlarged. (B) Histological examination reveals that the tumor is papillary adenocarcinoma. (C) p53-immunohistochemistry shows that most of cancer cells have nuclear positive reaction against p53. Note: a few normal tubal epithelial cells are also positive for p53 in their nucleus. (B) H & E stain, bars = 200 μm (low magnification) and 20 μm (high magnification) and (C) p53 immunohistochemistry, bar = 20 μm.
Figure 6. Macroscopic view of (A) lung tumor in the right upper lobe. Histopathologically, the tumor was (B) papillary adenocarcinoma (papillary 40%, acinar 20%, and lepidic 40%) without metastases. (B) H & E stain, bar = 50 μm.
Primary fallopian tube carcinoma is very rare with an average of 0.3% of all the gynecological malignancies  , although its exact frequency may be underestimated because it has the same pathological features as serous epithelial ovarian cancer or primary peritoneal serous carcinoma  . Tubal carcinomas commonly develop in postmenopausal women in their fifth or sixth decades. Most of the patients are asymptomatic or they tend to present with certain non-specific symptoms. In most of these patients, infertility and chronic pelvic disease are commonly associated. Because the lumen of fallopian tubes anatomically continues with uterine cavity, cancer cells developed in the fallopian tube could easily reach to uterine cavity, when compared to ovarian cancer. Therefore, endometrial Pap smears show positive results at early stage of tubal cancer  . In the present case, we could not perform endometrial Pap smears because of closed cervical duct. However, based on the positive results vaginal smears, we suspected gynecological malignancy, possibly fallopian tube cancer, and did abdominal simple hysterectomy and bilateral appendages extirpation with removal of para-aortic lymph nodes. As a result, we detected the right tubal adenocarcinoma. Our findings suggest that in addition to endometrial and cervical cytology, vaginal cytology is useful for early detection of fallopian tube cancer  .
When compared to endometrial cancer, adenocarcinoma cells originated from fallopian tube on the endometrial Pap smears have been reported to show several characteristics: 1) background is clear or clean; 2) number of atypical cells is small; 3) clusters of atypical cells are papillary or small spherical pattern; 4) atypical cell are frequently degenerated; and 5) neutrophils in the cytoplasm of atypical cells are infrequent  . In our case, cancer cells were found as papillary clusters with clear background on the vaginal smear. Cytological specimens obtained by hydrotubation were reported to be useful for diagnosis adenocarcinoma in situ in the fallopian tube  . Chen et al.  have recently developed a standard procedure to collect fallopian tube brushing from fresh surgical specimens. They have proved that tubal brushing cytology is useful for early detection of serous tubal intraepithelial carcinoma (STIC).
Primary fallopian tube carcinoma is rarely suspected preoperatively and resembles histologically and clinically primary ovarian carcinoma  . The diagnostic criteria for primary fallopian tube cancer, which were proposed by Hu et al.  , were revised by Sedis   . They include: 1) main tumor should develop in the fallopian tube; 2) the histological pattern reproduces the epithelium of the tubal epithelium; 3) transition from benign to malignant tubal epithelium is demonstrable; and 4) ovaries or endometrium are either normal or contain a tumor that is smaller than the tumor in the tube. More than 90% of primary fallopian tube carcinoma was papillary adenocarcinoma  . Histopathology of our case satisfied these two criteria.
Based on these two criteria, the fallopian tube cancer has been considered to be extremely rare at 0.5% - 03% of all gynecological neoplasms  . However, the concept that serous tubal intraepithelial carcinoma (STIC) is a precursor lesion of high grade serous adenocarcinoma (HGSA) has recently been proposed  . As presented, vaginal cytology was useful for detecting tubal adenocarcinoma. If STIC is a precursor lesion of HGSA, endometrial and cervical cytological diagnosis may show an important role in early diagnosis of HGSC  . We have recently experienced a case of STIC cytologically diagnosed during the operation  .
Immunohistochemical expression of p53 in the tubal epithelium is known to be an early biomarker of tubal malignancy    . In our case, most of adenocarcinoma cells in the right tube were immunohistochemically positive for p53. Interestingly, normal-looking tubal epithelial cells were also positive for p53. These findings may suggest that histopathologically normal tubal epithelium already has genetic alteration.
We thank the editor and reviewers for the constructive comments, which helped us to improve the manuscript. This case report was approved by the patient verbal consent.
The authors declare that they have no competing interest.