JBM  Vol.6 No.6 , June 2018
Volatile Compounds Analysis of Tunisian Propolis and Its Antifungal Activity
Abstract: In this study, four samples of Tunisian propolis were analyzed for their volatile compounds. Static Headspace technique coupled with gas chromatography-mass spectrometry (GC/MS) was used for the volatile profile characterization. Statistical investigation of the samples was made applying hierarchical clustering method, K-Means cluster and principal component analysis. Results showed that 47, 36, 30 and 24 different compounds were identified, respectively, in the samples from Zouarine, Zelligua, Bizerte and Beni Khalled. Propolis volatiles were dominated by monoterpene hydrocarbons. α-pinene was the major compound representing 81.14%, 82.67% and 90.74%, respectively, of the total propolis volatiles collected from Zelligua, Beni Khalled and Zouarine and only 45.22% of the sample from Bizerte which had a very different composition. The in vitro antifungal activity of the volatiles from all samples against Candida albicans was also assayed and reported.
Cite this paper: Jihene, A. , Karoui, I. , Ameni, A. , Hammami, M. and Abderrabba, M. (2018) Volatile Compounds Analysis of Tunisian Propolis and Its Antifungal Activity. Journal of Biosciences and Medicines, 6, 115-131. doi: 10.4236/jbm.2018.66009.

[1]   Marcucci, M.C. (1995) Propolis: Chemical Composition, Biological Properties and Therapeutic Activity. Apidologie, 26, 83-99.

[2]   Burdock, G.A. (1998) Review of the Biological Properties and Toxicity of Bee Propolis. Food and Chemical Toxicology, 36, 347-363.

[3]   Alencar, S.M., Oldoni, T.L., Castro, M.L., Cabral, I.S., Costa-Neto, C.M., Cury, J.A., Rosalen, P.L. and Ikegaki, M. (2007) Chemical Composition and Biological Activity of a New Type of Brazilian Propolis: Red Propolis. Journal of Ethnopharmacology, 113, 278-283.

[4]   Sforcin, J.M. (2007) Propolis and the Immune System: A Review. Journal of Ethnopharmacology, 113, 1-14.

[5]   Kalogeropoulos, N., Konteles, S.J., Troullidou, E., Mourtzinos, I. and Karathanos, V.T. (2009) Chemical Composition, Antioxidant Activity and Antimicrobial Properties of Propolis Extracts from Greece and Cyprus. Food Chemistry, 116, 452-461.

[6]   Lu, L.C., Chen, Y.W. and Chou, C.C. (2005) Antibacterial Activity of Propolis against Staphylococcus aureus. International Journal of Food Microbiology, 102, 213-220.

[7]   da Cunha, M.G., Franchin, M., Galvao, L., de Ruiz, A., de Carvalho, J.E., Ikegaki, M., de Alencar, S.M., Koo, H. and Rosalen, P.L. (2013) Antimicrobial and Antiproliferative Activities of Stingless Bee Melipona scutellaris Geopropolis. BMC Complementary and Alternative Medicine, 13, 23.

[8]   Silici, S. and Kutluca, S. (2005) Chemical Composition and Antibacterial Activity of Propolis Collected by Three Different Races of Honeybees in the Same Region. Journal of Ethnopharmacology, 99, 69-73.

[9]   Bankova, V., Boudourova-Krasteva, G., Popov, S., Sforcin, J.M. and Funari, S.R.C. (1998) Seasonal Variations in Essential Oil from Brazilian Propolis. Journal of Essential Oil Research, 10, 693-696.

[10]   Sforcin, J.M., Fernandes, A., Lopes, C.A.M., Bankova, V. and Funari, S.R.C. (2000) Seasonal Effect on Brazilian Propolis Antibacterial Activity. Journal of Ethno-pharmacology, 73, 243-249.

[11]   Bankova, V. (2009) Chemical Diversity of Propolis Makes It a Valuable Source of New Biologically Active Compounds. Journal of ApiProduct and ApiMedical Science, 1, 23-28.

[12]   Bankova, V.S., de Castro, S.L. and Marcucci, M.C. (2000) Propolis: Recent Advances in Chemistry and Plant Origin. Apidologie, 31, 3.

[13]   Borcic, I., Radonic, A. and Grzunov, K. (1998) and Comparison of the Volatile Constituents of Propolis Gathered in Different Regions of Croatia. Flavour and Fragrance Journal, 11, 311-313.<311::AID-FFJ581>3.0.CO;2-X

[14]   Melliou, E., Stratis, E. and Chinou, I. (2007) Volatile Constituents of Propolis from Various Regions of Greece-Antimicrobial Activity. Food Chemistry, 103, 375-380.

[15]   Haile, K., Kebede, T. and Dekebo, A. (2012) A Comparative Study of Volatile Components of Propolis (Bee Glue) Collected from Haramaya. Bulletin of the Chemical Society of Ethiopia, 26, 353-360.

[16]   Pino, J.A., Marbot, R., Delgado, A., Zumárraga, C. and Sauri, E. (2006) Volatile Constituents of Propolis from Honey Bees and Stingless Bees from Yucatan. Journal of Essential Oil Research, 18, 53.

[17]   Kaskoniene, V., Kaskonas, P., Maruska, A. and Kubiliene, L. (2014) Chemometric Analysis of Volatiles of Propolis from Different Regions Using Static Headspace GC-MS. Central European Journal of Chemistry, 12, 736-746.

[18]   Naik, D.G., Vaidya, H.S. and Namjoshi, T.P. (2013) Essential Oil of Indian Propolis: Chemical Composition and Repellency against the Honeybee Apisflorea. Chemistry & Biodiversity, 10, 649-657.

[19]   Bankova, V., Christov, R., Pureb, O. and Bocari, G. (1994) Volatile Constituents of Propolis. Zeitschrift für. Naturforschung, 49, 6-10.

[20]   Segueni, N., Khadraoui, F., Moussaoui, F., Zellagui, A., Guerraf, N., Lahouel, M. and Rhouati, S. (2010) Volatils Constituents of Algerian Propolis. Annals of Biological Research, 1, 103-107.

[21]   Martos, I., Cossentini, M., Ferreres, F. and Tomás-Barberán, F.A. (1997) Flavonoid Composition of Tunisian Honeys and Propolis. Journal of Agricultural Food Chemistry, 45, 2824-2829.

[22]   Kouidhi, B., Zmantar, T. and Bakhrouf, A. (2010) Anti-Cariogenic and Anti-Bio-films Activity of Tunisian Propolis Extract and Its Potential Protective Effect against Cancer Cells Proliferation. Anaerobe, 16, 566-571.

[23]   Marcos-Arias, C., Eraso, E., Madariaga, L. and Quindós, G. (2011) In Vitro Activities of Natural Products against Oral Candida Isolates from Denture Wearers. BMC Complementary and Alternative Medicine, 11, 119.

[24]   Iosif, L., Preoteasa, C.T., Murariu-Maqureanu, C. and Preoteasa, E. (2016) Clinical Study on Thermography, as Modern Investigation Method for Candida-Associated Denture Stomatitis. Romanian Journal of Morphology and Embryology, 57, 191-195.

[25]   Barbouche, N. (2009) La filiére apicole en Tunisie, situation, contraintes et perspectives. Institut National d’agriculture, Barkane.

[26]   XLSTAT 2015 Software.

[27]   De Albuquerque, I.L., Alves, L.A., Lemos, T.L.G., Dorneles, C.A. and de Morais, M.O. (2008) Constituents of the Essential Oil of Brazilian Green Propolis from Brazil. Journal of Essential Oil Research, 20, 414-415.

[28]   Cheng, H., Qin, Z.H., Guo, X.F., Hu, X.S. and Wu, J.H. (2013) Geographical Origin Identification of Propolis Using GC-MS and Electronic Nose Combined with Principal Component Analysis. Food Research International, 51, 813.

[29]   Winston, M.L. (1987) The Biology of Honey Bees. Harvard University Press, Cambridge.

[30]   Getz, W.M. and Page, R.E. (1991) Chemosensory Kin-Communication Systems and Kin Recognition in Honey Bees. Ethology, 87, 298-315.

[31]   Dorman, H.J.D. and Deans, S.G. (2008) Antimicrobial Agents from Plants: Antibacterial Activity of Plant Volatile Oils. Journal of Applied Microbiology, 88, 308-316.

[32]   Oliveira, A.P., Franca, H.S., Kuster, R.M., Teixeira, L.A. and Rocha, L.M. (2010) Chemical Composition and Antibacterial Activity of Brazilian Propolis Essential Oil. Journal of Venomous Animals and Toxins including Tropical Diseases, 16, 121-130.

[33]   Maróstica Junior, M.R., Daugsch, A., Moraes, C.S., Queiroga, C.L., Pastore, G.M. and Park, Y.K. (2008) Brazilian Red Propolis-Chemical Composition and Botanical Origin. Ciência e Tecnologia de Alimentos, 28, 178.

[34]   Nunes, C.A. and Guerreiro, M.C. (2012) Characterization of Brazilian Green Propolis throughout the Seasons by Headspace GC/MS and ESI-MS. Journal of the Science of Food and Agriculture, 92, 433-438.

[35]   Silva, V., Genta, G., Moller, M.N., Masner, M., Thomson, L., Romero, N., Radi, R., Fernandes, D.C., Laurindo, F.R., Heinzen, H., Fierro, W. and Denicola, A. (2011) Antioxidant Activity of Uruguayan Propolis. In Vitro and Cellular Assays. Journal of Agricultural and Food Chemistry, 59, 6430-6437.

[36]   Simionatto, E., Facco, A.T., Morel, A.F., Giacomelli, S.R. and Linares, C.E.B. (2012) Chiral Analysis of Monoterpenes in Volatile Oils from Propolis. Journal of the Chilean Chemical Society, 57, 1240-1243.

[37]   Zhou, H., Tao, N. and Jia, L. (2014) Antifungal Activity of Citral, Octanal and α-Terpineol against Geotrichum citri-aurantii. Food Control, 37, 277-283.

[38]   Li, Y.J., Xuan, H.Z., Shou, Q.Y., Zhan, Z.G., Lu, X. and Hu, F.L. (2012) Therapeutic Effects of Propolis Essential Oil on Anxiety of Restraint-Stressed Mice. Human & Experimental Toxicology, 31, 157-165.

[39]   Magiatis, P., Melliou, E., Skaltsounis, A.-L., Chinou, I. and Mitaku, S. (1999) Chemical Composition and Antimicrobial Activity of the Essential Oils of Pistacia lentiscus var. chia. Planta Medica, 65, 749-752.

[40]   Hartati, R., Suganda, A.G. and Fidrianny, I. (2015) Chemical Composition and Antimicrobial Activity of Diterpene and Essential Oils of Hedychium roxburghii Blume Rhizome. Asian Journal of Pharmaceutical and Clinical Research, 8, 221-226.