Health  Vol.10 No.6 , June 2018
Antimicrobial Resistance and Plasmid Profiles of Salmonella enterica Serovars from Different Sources in Lagos, Nigeria
Abstract: Introduction: Salmonella enterica Serovars remains one of the leading pathogens that cause diarrhoea and bloodstream infections in developing countries. The emergence of multidrug resistant (MDR) Salmonella has become a serious problem globally. This study investigated the antibiotic resistance and plasmid profiles of Salmonella isolates from different sources. Methods: Seventy-three samples comprised of clinical (30), hand swab (15), food (10) and water (18) were analyzed bacteriologically. Salmonella isolates were identified and characterized by standard procedures. Isolates were subjected to antimicrobial susceptibility testing and were further screened for plasmid DNA by standard methods. Results: A total of 27 Salmonella isolates made up of 5 (18.5%) S. typhi, 6 (22.2%) S. enteritidis, 9 (33.3) S. typhimurium, 5 (18.5%) S. cholerasuis, and 1 (3.7%) each of S.arizonae and S. vichow were obtained in this study. All the isolates developed resistance to three or more antibiotics evaluated. Four distinct resistance profiles: TetAmpCol, TetAmpColCot, TetAmpColCip and TetAmpColCotCip were recorded with 63% of the isolates exhibiting resistance profile TetAmpColCot. Specifically 23 of 27 (85.2%) of the isolates harboured plasmid DNA comprised of 12 distinct plasmid profiles of different sizes ranging from 3.2 kb to 30.2kb. Salmonella isolates of the same species from different sources differed in plasmid profile. Plasmid profile was found to show good discriminatory capability compared to antibiotics resistance profile. Conclusion: This study revealed that both resistance antibiogram and plasmid profile are still viable epidemiological tools for tracing the source of Salmonella isolates. A need for prudent use of antibiotics is suggested.
Cite this paper: Akinyemi, K. , Ajoseh, S. , Iwalokun, B. , Oyefolu, A. , Fakorede, C. , Abegunrin, R. and Adunmo, O. (2018) Antimicrobial Resistance and Plasmid Profiles of Salmonella enterica Serovars from Different Sources in Lagos, Nigeria. Health, 10, 758-772. doi: 10.4236/health.2018.106058.

[1]   Salehi, T.Z., Mahzounieh, M. and Saeedzadeh, A. (2005) Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method. International Journal of Poultry Science, 4, 557-559.

[2]   Popoff, M.Y., Parry, C.M. and Le Minor, L. (1997) Antigenic Formula of the Salmonella Serovars. 7th Revision, World Health Organisation, A Consorting Centre for Reference and Research on Salmonella, Pasteur Institute, Paris, 56-87.

[3]   Grimont, P.A.D. and Weill, F.X. (2007) Antigenic Formulae of the Salmonella serovars. 9th Edition, WHO Collaborating Center for Reference and Research on Salmonella at Institute Pasteur in France, Paris, 150 p.

[4]   CDC (2010) Salmonella.

[5]   Swartz, M.N. (2002) Human Diseases Caused by Food-Borne Pathogens of Animal Origin. Clinical Infectious Diseases, 34, 111-122.

[6]   Lazaro, N.A., Tibana, D.P., Rodrigues, E., Reis, B.Q. and Hofer, E. (2004) Antimicrobial Resistance and R-Plasmids in Salmonella spp. from Swine and Abattoir Environment. Pesquisa Veterinária Brasileira, 24, 57-60.

[7]   Health Organisation (WHO) (2014a) Antimicrobial Resistance Global Report on Surveillance. X1-28.

[8]   Crump, J.A., Luby, S.P. and Minz, E.D. (2003) The Global Burden of typhoid Fever. Bulletin of the World Health Organization, 82, 346-353.

[9]   Crump, J.A. and Mintz, E.D. (2010) Global Trends in Typhoid and Paratyphoid Fever. Clinical Infectious Diseases, 50, 241-246.

[10]   Morpeth, S.C., Ramadhani, H.O. and Crump, J.A. (2009) Invasive Non-Typhi Salmonella Disease in Africa. Clinical Infectious Diseases, 49, 606-611.

[11]   Reddy, E.A., Shaw, A.V. and Crump, J.A. (2010) Community-Acquired Bloodstream Infections in Africa: A Systematic Review and Meta-Analysis. The Lancet Infectious Diseases, 10, 417-432.

[12]   Basnyat, B., Maskey, A.P., Zimmerman, M.D. and Murdoch, D.R. (2005) Enteric (Typhoid) Fever in Travelers. Clinical Infectious Diseases, 41, 1467-1472.

[13]   Chau, T.T., Campbell, J.I., Galindo, C.M., Hoang, N.V., et al. (2007) Antimicrobial Drug Resistance of Salmonella enterica Serovar Typhi in Asia and Molecular Mechanisms of Reduced Susceptibility to the Fluoroquinolones. Antimicrobial Agents and Chemotherapy, 51, 4315-4323.

[14]   Parry, C.M. and Threlfall, E.J. (2008) Antimicrobial Resistance in Typhoidal and Nontyphoidal Salmonellae. Current Opinion in Infectious Diseases, 21, 531-538.

[15]   Su, L.H., Chiu, C.H., Chu, C. and Ou, J.T. (2004) Antimicrobial Resistance in Nontyphoid Salmonella Serotypes: A Global Challenge. Clinical Infectious Diseases, 39, 546-551.

[16]   Adeshina, G.O., Osuagwu, N., Okeke, C.E. and Ehinmidu, J.O. (2010) Plasmid DNA Analysis and Conjugative Study of Antibiotics Resistant Salmonella typhi and paratyphi to Ten Selected Antibiotics in Zaria, Nigeria. African Journal of Pharmacy and Pharmacology, 4, 151-157.

[17]   El-Sayed, M.A., Tawakol, W.M., Ragab, Y.M. and Amin, M.A. (2012) Phenotypic Detection of Potential Antimicrobial Resistance among Environmental Salmonella Species. International Journal of Microbiology Research, 3, 38-45.

[18]   Akinyemi, K.O., Iwalokun, B.A., Alafe, O.O., Mudashiru, S.A. and Fakorede, C. (2015) BlaCTX-M-1 Group Extended Spectrum Beta Lactamase-Producing Salmonella typhi from Hospitalized Patients in Lagos, Nigeria. Infection and Drug Resistance, 2015, 99-106.

[19]   Miriagou, V., Carattoli, A. and Fanning, S. (2006) Antimicrobial Resistance Islands: Resistance Gene Clusters in Salmonella Chromosome and Plasmids. Microbes and Infection, 8, 1923-1930.

[20]   Murray, B.E. (1986) Resistance of Shigella, Salmonella and Other Selected Enteric Pathogens to Antimicrobial Agents. Reviews of Infectious Diseases, 8, 172-181.

[21]   Health Organisation (WHO) (2014) Briefing Note-Antimicrobial Resistance: An Emerging Water Sanitation and Hygiene Issue. WHO/FWC/WSH/14.7.

[22]   Van, T.T.H., Moutafis, G., Istivan, T., Tran, L.T. and Coloe, P.J. (2007) Detection of Salmonella spp. in Retail Raw Food Samples from Vietnam and Characterization of Their Antibiotic Resistance. Applied and Environmental Microbiology, 73, 6885-6890.

[23]   Sun, M. (1984) In Search of Salmonella’s Smoking Gun. Science, 226, 30-32.

[24]   Glynn, M.K., Boppy, C., Dewitt, W., Dabney, P., Mokhtar, M. and Angulo, F.J. (1998) Emergence of Multi-Drug Resistant Salmonella enterica Serovar Typhimurium DT104 Infections in the United States. New England Journal of Medicine, 338, 1333-1338.

[25]   Carattoli, A., Filetici, E., Villa, L., Dionisi, A.M., Ricci, A. and Luzzi, I. (2002) Antibiotic Resistance Genes and Salmonella Genomic Island 1 in Salmonella enterica Serovar Typhimurium Isolated in Italy. Antimicrobial Agents and Chemotherapy, 46, 2821-2828.

[26]   Helms, M., Vastrup, P., Gerner-Smidt, P. and Molbak, K. (2003) Short and Long Term Mortality Associated with Foodborne Bacterial Gastrointestinal Infections: Registry Based Study. BMJ, 326, 357.

[27]   Tanxe, R. (1999) Salmonella Enteritis and Salmonella Typhimurium DT104: Successful Subtypes in the Modern World. In: Scheld, W.M., Craig, W.A. and Hughes, J.M., Eds., Emerging Infections, ASM Press, Washington, 37-52.

[28]   Davis, J. (1994) Inactivation of Antibiotics and Dissemination of Resistance Genes. Science, 264, 375-382.

[29]   Chu, C.H., Su, L.H. and Chiu, C. (2004) Salmonella enterica Serotype Choleraesuis: Epidemiology, Pathogenesis, Clinical Disease and Treatment. Clinical Microbiology Reviews, 17, 311-322.

[30]   Ben, S.I., Mzoughi, R. and Aouni, M. (2008) Laboratory Typing Methods for Diagnostic of Salmonella Strains, the “Old” Organism That Continued Challenges. Annals of Microbiology, 60, 1-25.

[31]   Mackie, T.J. and McCartney, J.E. (1989) Practical Medical Microbiology. In: College, J.C., Dugluid, J.P., Frasor, A.G. and Marmion, B.P., Eds., WHO Guideline for Drinking Water Quality, 13th Edition, Vol. 12, Churchill Livingstone, London, 910.

[32]   Tambekar, D.H., Shirsat, S.D., Suradkar, S.B., Rajankar, P.N. and Banginwar, Y.S. (2007) Prevention of Transmission of Infectious Disease: Studies on Hand Hygiene in Health-Care among Students. Continental Journal of Biomedical Sciences, 1, 6-10.

[33]   Clinical and Laboratory Standards Institute (CLSI) (2008) Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard—Third Edition. Document M31-A3, Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, 19087-1898.

[34]   Liu, S.T. (1981) Rapid Procedure for Detection and Isolation of Large and Small Plasmids. Journal of Bacteriology, 145, 1365-1373.

[35]   Meyers, J.A., Sanchez, D., Elwell, L.P. and Falkow, S. (1976) Simple Agarose Gel electrophoretic Method for the Identification and Characterization of Plasmid Deoxyribonucleic Acid. Journal of Bacteriology, 127, 1529-1537.

[36]   Anon (2015) Nice Antimicrobial Stewardship: Right Drug, Dose, and Time? Editorial. The Lancet, 386, 717.

[37]   David, O.M., Adelapo, D.A., Moro, D.D., Esan, C.O, Oje, O.J. and Famurewa, O. (2015) Prevalence and Carriage Status of Salmonella typhi among Students of Ekiti State University, Ado-Ekiti, Nigeria. International Journal of Biosciences, 6, 1-8.

[38]   Owoseni, A. and Onilude, A. (2016) Incidence and Plasmid Profiles of Multidrug Resistant Enterobacteria Isolates from Processed Convenience Foods in Some Countries of West Africa. Food and Public Health, 6, 44-51.

[39]   Majowicz, S.E., Musto, J. and Scallan, E. (2010) The International Collaboration on Enteric Disease “Burden of Illness” Studies: The Global Burden of Nontyphoidal Salmonella Gastroenteritis. Clinical Infectious Diseases, 50, 882-889.

[40]   Akinyemi, K.O., Fashola, M.O., Habib, N. and Akinwande, E. (2013) Vended Foods in Lagos, Nigeria: A Potential Reservoir for the Spread of Emerging Strains of Drug Resistant Bacteria. Health, 5, 675-680.

[41]   Umaru, G.A., Adamu, Z., Ishaya, D., Abubakar, Y.U., Hussaini, A., Umar, M., Adamu, S.G. and Adamu, N.B. (2015) Prevalence and Antimicrobial Resistance Pattern of Escherichia coli in Drinking Waters in Jalingo Metropolis, Taraba State, North-Eastern Nigeria. Microbiology Research International, 3, 8-13.

[42]   Kariuki, S., Revathi, G., Kariuki, N., et al. (2006) Invasive Multidrug-Resistant Non-Typhoidal Salmonella Infections in Africa: Zoonotic or Anthroponotic Transmission? Journal of Medical Microbiology, 55, 585-591.

[43]   Akinyemi, K.O., Oyefolu, A.O., Omonigbehin, E.O., Akinside, K.A. and Coker, A.O. (2000) Evaluation of Blood Collected from Clinically Diagnosed Typhoid Fever Patients in the Metropolis of Lagos, Nigeria. Journal of Nigerian Infection Control Association, 3, 25-30.

[44]   Okeke, O.C., Iroha, I.R., Nwadiogbu, I.A., Agumah, N., Eni, O. and Odinkemere, S.C. (2015) Antimicrobial Resistance Pattern of Coliform Bacteria Isolated from Sachet and Borehole Waters Sold in Abakaliki Metropolis of Ebonyi State, Nigeria. International Journal of Innovation and Scientific Research, 16, 526-532.

[45]   Fashae, K., Ogusola, F., Aerestrup, F.M. and Hendriksen, R.S. (2010) Antimicrobial Susceptibility and Serovars of Salmonella from Chicken and Human in Ibadan, Nigeria. The Journal of Infection in Developing Countries, 4, 484-494.

[46]   Ammari, S., Laglaoui, A., En-Nanei, L., Bertrand, S., Wildemauwe, C., Said, B. and Mohammed, A. (2009) Isolation, Drug Resistance and Molecular Characterization of Salmonella Isolates in Northern Morocco. The Journal of Infection in Developing Countries, 3, 41-49.

[47]   Ling, J.M., Chan, E.W.C. and Cheng, A.F. (2001) Molecular Epidemiological Analysis of Salmonella enterica Serotype Derby Infections in Hong Kong. Journal of Infection, 42, 145-153.

[48]   Wain, J. and Kidgell, C. (2004) The Emergence of Multidrug Resistance to Antimicrobial Agents for the Treatment of Typhoid Fever. Transactions of the Royal Society of Tropical Medicine and Hygiene, 98, 423-430.

[49]   Rychlik, I., Gregorova, D. and Hradecka, H. (2006) Distribution and Function of Plasmids in Salmonella enterica. Veterinary Microbiology, 112, 1-10.

[50]   Learn-Han, L., Yoke-Kqueen, C., Shiran, M.S., Sabrina, S., Noor-Zaleha, A.S., Sim, J.H., Chai-Hoon, K. and Son, R. (2009) Molecular Characterization and Antimicrobial Resistance Profiling of Salmonella enterica Subsp. enterica Isolated from “Selom” (Oenanthe stolonifera). International Food Research Journal, 16, 191-202.