AJMB  Vol.8 No.3 , July 2018
Utilizing Short Tandem Repeats (STRs) as a Resolving Matrix in Parental Dispute DNA Analysis
Abstract: Interest in DNA analysis using short tandem repeats (STR) as finger printing tools in forensic medicine has gained tremendous application, as expression of these nuclear factors have enhanced forensic examination. Here we used this Biochemical characterization after conventional extraction process, polymerase chain reaction (PCR), gel electrophoresiss and a sequencer to distinguish and resolve parental dispute. The differential migration of labeled DNA fragments which attains excitation energy with a laser elicits fluorescent light of different wavelength depending on the dye used. A data collection software (Genemapper) collects raw data (spectrograph) and converts it to an electropherogram that is interpreted. By comparing the DNA profiles, inclusion and exclusion criteria were elucidated to resolve disputes. The inherent discriminating power of STRs used in analysis enhances resolution of cell mixtures, genetic aberration, substantiation of tissue origin and provides genetic distinction which is a robust and reliable approach in resolving parental disputes.
Cite this paper: Simeon, G. and Olukemi, A. (2018) Utilizing Short Tandem Repeats (STRs) as a Resolving Matrix in Parental Dispute DNA Analysis. American Journal of Molecular Biology, 8, 156-165. doi: 10.4236/ajmb.2018.83013.

[1]   Butler, J.M. (2005) Forensic DNA Typing: Biology Technology and Genetics of STR Markers. 2nd Edition, Elsevier Academic Press, New York.

[2]   Butler, J.M. (2006) Genetics and Genomics of Core STR Used in Human Identity Testing. Journal of Forensic Sciences, 51, 253-265.

[3]   Collins, P.J., Henessy, L.P., Leibelt, C.S., Roby, R.K., Reeder, D.J. and Foxal, P.A. (2004) Developmental Validation of a Single-Tube Amplification of the 13 CODSIS STR Loci, D2S1338, D19S433, and Amelogenin: The AmpF/STR® Identifier PCR® Amplification Kit. Journal of Forensic Sciences, 49, 1265-1277.

[4]   Scientific Working Group on DNA Analysis Methods (SWGDAM) (2000) Short Tandem Repeat STR Interpretation Guidelines.

[5]   Whitaker, et al. (1995) The Forensic Science Service in Running a Degraded DNA Samples from Remains of Victims of the Branch Davidian Fire in Waw, Texas Find That Smaller STRs in Their 4-Plex Work Better than Larger Loci. BioTechniques, 18, 670-677.

[6]   Dixon, L.A., Dobbins, A.E., Paulker, H.K., Butler, J.M., Vallone, P.M., Coble, M.D., Parson, W. and Berger, B. (2006) Analysis of Artificially Degraded DNA Using STRs and SNPs—Result of a Collaborative European (EDNAP) Exercise. Forensic Science International, 164, 33-44.

[7]   Gill, P. (2002) Role of Short Tandem Repeat DNA in Forensic Casework in the UK—Past, Present and Future Perspectives. BioTechniques, 32, 366-370.

[8]   Gill, P., Werrett, D.J., Budowle, B. and Guerrieri, R. (2004) An Assessment of Wether SNPs Will Replace STRs in National Databases Joint Considerations of the DNA Working Group of the European Network of Forensic Science Institute (ENFSI) and the Scientific Working Groups of DNA Analysis Methods (SWGDAM). Science & Justice, 44, 51-53.

[9]   Gjertson, D.W., Brenner, C.H., Bau, M.P., Carracedo, A., Guidet, F., Luque, J.A., Lessig, R., Mayr, W.R., Pascali, V.L., Prinz, M., Schneider, P.M. and Morling, N. (2007) ISFG: Recommendation on Biostatistics in Paternity Testing. Forensic Science International: Genetics, 1, 223-231.

[10]   Butler, J.M., Buel, E., Crivellente, F. and McCord, B.R. (2004) Forensic DNA Typing by Capillary Electrophoresis Using ABI Prism 310 Are 3100 Genetic Analyzers for STR Analysis. Electrophoresis, 25, 1397-1412.

[11]   Norton, A. and Ozzie, Z. (2008) Which Half Is Mommy?: Tettragametic Chimerism and Trans-Subjectivity. Woman’s Studies Quarterly, 36, 106-125.

[12]   Liu, P., Seo, T.S., Beyor, N., Shin. R.J., Scherer, J.R. and Mathies, R.A. (2007) Integrated Potable Polymerase Chain Reaction-Capillary Electrophoresis Microsystem for Rapid Forensic Short Tandem Repeat Typing. Analytical Chemistry, 79, 1181-1889.

[13]   Butler, J.M., Li, J., Shaler, T.A., Moriforte, J.A. and Becker, C.H. (1998) Reliable Genotyping of Short Tandem Repeat Loci without an Ellelic Ladder Using Time-of-Flight Mass Spectrometry. International Journal of Legal Medicine, 112, 45-49.

[14]   Giusti, W.G. and Adriano, T. (1993) Synthesis and Characterization of 5’-Fluorescent-Dye-Labeled Oligonucleotides. Genome Research, 2, 223-227.

[15]   Oberacher, H., Pitterl, F., Huber, G., Niederstatter, H., Steinlehner, M. and Parson, W. (2008) Increased Forensic Efficiency of DNA Finger Prints through Simultaneous Resolution of Length and Nucleotide Variability by High-Performance Mass Spectrometry. Human Mutation, 29, 427-432.

[16]   Wiegand, P. and Klieber, M. (2000) Less Is More—Length Reduction of STR Amplicons Using Redesigned Primers. International Journal of Legal Medicine, 114, 285-287.

[17]   Butler, J.M., Sher, Y. and McCord, B.R. (2003) The Development of Reduced Sized STR Amplicons as Tools for Analysis Degraded DNA. Journal of Forensic Sciences, 48, 1054-1064.

[18]   John, M.B., Eric, B. and Bruce, R.M. (2004) Forensic DNA Typing by Capillary Electrophoresis Using the ABI Prism 310 and 3100 Genetic Analyzers for STR Analysis. Electrophoresis, 25, 1397-1412.

[19]   Budowled, B., Van Daal, A. (2009) Extracting Evidence from Forensic DNA Analysis: Future Molecular Biology Directions. BioTechniques, 46, 339-340.

[20]   Anderson, H., Fujimori, S., Ota, M., Oki, T. and Fukushima, H. (2008) Evaluation of MiniY-STR Multiplex PCR Systems for Extended 16 Y-STR Loci. International Journal of Legal Medicine, 122, 43-49.

[21]   Bonnet, J., Colotte, M., Coudy, D., Couallier, V., Porter, J., Morin, B. and Tuffet, S. (2010) Chain and Conformation Stability of Solid-State DNA: Implication for Room Temperature Storage. Nucleic Acids Research, 38, 1531-1546.

[22]   Gill, P., Whitaker, J., Flaxman, C., Brown, N. and Buckleton, J. (2000) An Investigation of the Rigor of Interpretation Rules for STRs Derived from Less than 100 pg of DNA. Forensic Science International, 112, 17-40.

[23]   National Institute of Justice (NIJ) (2000) The Future of Forensic DNA Testing: Predictions of the Research and Development Working Group of the National Commission on the Future of DNA Evidence. Washington DC.

[24]   Hill, B. (2011) Characterization of the 26 Mini STR Loci under Development of the NIST. Promega Meeting, Nashville.

[25]   Hill, C.R., Kline, M.C., Mulero, J.J., Lagale, R.E., Chang, C.W., Henessy, L.K. and Butler, J.M. (2007)) Concordance Study between the AmpF/STR® MumiFilerTM PCR Amplification Kit and Conventional STR Typing Kits. Journal of Forensic Sciences, 52, 870-873.

[26]   Leibelt, C., Budorole, B., Collins, P., Daoudi, Y., Morreti, T., Nunn, G., Reeder, D. and Roby, R. (2003) Identification of a D8S1179 Primer Binding Site Mutation and the Validation of a Primer Designed to Recover Null Alleles. Forensic Science International, 133, 220-227.

[27]   Lederer, T., Braunschweiger, G., Betz, P. and Seidl, S. (2002) Purification of STR-Multiplex Amplified Microsamples Can Enhance Signal Intensity in Capillary Electrophoresis. International Journal of Legal Medicine, 116, 165-169.

[28]   Hofreiter, M., Serre, D., Poiriar, H.N., Kuch, M. and Paabo, S. (2001) Ancient DNA. Nature Reviews Genetics, 2, 353-359.

[29]   Hansnon, E.K. and Ballentyne, J. (2005) Whole Genome Amplification Strategy for Forensic Genetic Analysis Using Single or Few Cell Equivalent Genomic DNA. Analytical Biochemistry, 346, 246-257.

[30]   Phipps, M. and Petricevics, S. (2007) The Tendency of Individuals to Transfer DNA to Handled Items. Forensic Science International, 168, 162-168.