ABSTRACT An efficient method of isolating an anti-cancer sesquiterpene lactone, eremophila-1(10)-11(13)-dien-12,8?- olide, was developed from fresh Calomeria amaranthoides plant material on the basis of its non-polar vola-tile property. Steam distillation of fresh plant material gave a high recovery of sesquiterpene-rich oil, 0.56%, compared with the estimated 0.66% calculated from solvent extraction of dried plant material. The ses-quiterpene-rich oil containing 58% of the sesquiterpene lactone was fractionated by the short-column vac-uum chromatography method, using minimal stationary-phase and solvent, to give the sesquiterpene lactone 95% purity, yield 41%.
Cite this paper
nullC. Duke, C. Haaften and V. Tran, "Efficient Isolation of an Anti-Cancer Sesquiterpene Lactone from Calomeria amaranthoides by Steam Distillation," Green and Sustainable Chemistry, Vol. 1 No. 4, 2011, pp. 123-127. doi: 10.4236/gsc.2011.14020.
 C. F. Puttock, “Flora of Victoria,” In: N. G. Walsh and T. J. Entwisle, Eds,, Inkata Press, Melbourne, Vol. 4, 1999, p. 1.
 C. Zdero, F. Bohlmann, A. Anderberg and R. M. King, “Eremophilane Derivatives and Other Constituents from Haeckeria Species and Further Australian Inuleae,” Phytochemistry, Vol. 30, No. 8, 1991, pp. 2643-2650.
 C. van Haaften, C. C. Duke, A. M. Weerheim, N. P. M. Smit, P. M. M. van Haard, F. Darroudi and B. J. M. Z. Trimbos, “Potent Cytotoxic Effects of Calomeria Amaranthoides on Ovarian Cancers,” Journal of Experimental & Clinical Cancer Research, Vol. 30, No. 1, 2011, p. 29.
 N. Tanaka, T. Yazawa, K. Aoyama and T. Murakami, “Chemical Studies on the Constituents of Xanthium Canadense Mill,” Chemical & Pharmaceutical Bulletin, Vol. 24, 1976, pp. 1419-1421.
 F. Bohlmann, C. Zdero and M. Silva, “Two Further Ere- mophilane Derivatives from Tessaria Absynthioides,” Phytochemistry, Vol. 16, 1977, pp. 1302-1303.
 H. Oh, P. R. Jensen, B. T. Murphy, C. Fiorilla, J. F. Sullivan, T. Ramsey and W. Fenical, “Cryptosphaerolide, a Cytotoxic Mcl-1 Inhibitor from a Marine-Derived Ascomycete Related to the Genus Cryptosphaeria,” Journal of Natural Products, Vol. 73, No. 5, 2010, pp. 998-1001.
 J. C. Spitzer and C. Steelink, “Isolation of Matricin from Artemisia Caruthii,” Journal of Pharmaceutical Sciences, Vol. 56, No. 5, 1967, pp. 650-651.
 C. C. Duke and R. J. Wells, “Investigation of Readily Available Chiral Compounds for Preparative Scale Resolutions,” Australian Journal of Chemistry, Vol. 40, No. 10, 1987, pp. 1641-1654.