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 JBM  Vol.6 No.5 , May 2018
Deep Exploration of Bifidobacteria through Metabolomics Study
Abstract: Bifidobacteria are probiotic bacteria with multiple health-promoting properties for human being. The global market for probiotics, especially for bifidobacteria is booming. However, the entire market is still at an early stage as there is nearly no fine products developed yet except the whole bacterial cells. The maturation of metabolomics technologies make it possible to study complex mixture with high-throughput, comprehensive maps and libraries. Therefore, we prospect that metabolomics studies mainly based on liquid/gas chromatography-mass spectrometry (LC/GC-MS) can deepen our understanding in detail during the study of metabolic mechanisms of bifidobacteria. These studies can be conducted at three phases, including non-targeted, targeted metabolomic analysis of bifidobacteria, and specific metabolites production through metabolic engineering and fermentation. Metabolomic studies of bifidobacteria will allow us to fully explore their metabolic mechanisms and to utilize metabolites that contribute to human health. In particular, bifidobacteria derived conjugated linoleic acids and bacteriocins are two kinds of fined products that may have great potentials in the future and can be used as food additives.
Cite this paper: Li, J. , Jiang, Y. , Shen, Y. , Li, Q. and Sun, Z. (2018) Deep Exploration of Bifidobacteria through Metabolomics Study. Journal of Biosciences and Medicines, 6, 57-62. doi: 10.4236/jbm.2018.65008.
References

[1]   Sun, Z. (2014) Development of Gene Expression Systems in Bifidobacterium bifidum S17 and Their Application for Tumor Therapy. Dissertation, Open Access Repositorium der Universitat Ulm, Ulm.

[2]   Bazanella, M., Maier, T.V., Clavel, T., et al. (2017) Randomized Controlled Trial on the Impact of Early-Life Intervention with Bifidobacteria on the Healthy Infant Fecal Microbiota and Metabolome. American Journal of Clinical Nutrition, 106, 1274-1286.

[3]   Grimm, V., Westermann, C. and Riedel, C.U. (2014) Bifidobacteria-Host Interactions—An Update on Colonisation Factors. BioMed Research International, 2014, 10.
https://doi.org/10.1155/2014/960826

[4]   Sanchez, B., Urdaci, M.C. and Margolles, A. (2010) Extracellular Proteins Secreted by Probiotic Bacteria as Mediators of Effects That Promote Mucosa-Bacteria Interactions. Microbiology, 156, 3232-3242.
https://doi.org/10.1099/mic.0.044057-0

[5]   Guglielmetti, S., Mora, D., Gschwender, M., et al. (2011) Randomised Clinical Trial: Bifidobacterium bifidum MIMBb75 Significantly Alleviates Irritable Bowel Syndrome and Improves Quality of Life—A Double-Blind, Placebo-Controlled Study. Alimentary Pharmacology & Therapeutics, 33, 1123-1132.
https://doi.org/10.1111/j.1365-2036.2011.04633.x

[6]   Cronin, M., Morrissey, D., Rajendran, S., et al. (2010) Orally Administered Bifidobacteria as Vehicles for Delivery of Agents to Systemic Tumors. Molecular Therapy, 18, 1397-1407.
https://doi.org/10.1038/mt.2010.59

[7]   Sulek, K., Vigsnaes, L.K., Schmidt, L.R., et al. (2014) A Combined Metabolomic and Phylogenetic Study Reveals Putatively Prebiotic Effects of High Molecular Weight Arabino-Oligosaccharides When Assessed by in Vitro Fermentation in Bacterial Communities Derived from Humans. Anaerobe, 28, 68-77.
https://doi.org/10.1016/j.anaerobe.2014.05.007

[8]   Hong, Y.S., Hong, K.S., Park, M.H., et al. (2011) Metabonomic Understanding of Probiotic Effects in Humans with Irritable Bowel Syndrome. Journal of Clinical Gastroenterology, 45, 415-525.
https://doi.org/10.1097/MCG.0b013e318207f76c

[9]   Russell, D.A., Ross, R.P., Fitzgerald, G.F., et al. (2011) Metabolic Activities and Probiotic Potential of Bifidobacteria. International Journal of Food Microbiology, 149, 88-105.
https://doi.org/10.1016/j.ijfoodmicro.2011.06.003

[10]   O’Shea, E.F., Cotter, P.D., Stanton, C., et al. (2012) Production of Bioactive Substances by Intestinal Bacteria as a Basis for Explaining Probiotic Mechanisms: Bacteriocins and Conjugated Linoleic Acid. International Journal of Food Microbiology, 152, 189-205.
https://doi.org/10.1016/j.ijfoodmicro.2011.05.025

[11]   Gorissen, L., De Vuyst, L., Raes, K., et al. (2012) Conjugated Linoleic and Linolenic Acid Production Kinetics by Bifidobacteria Differ among Strains. International Journal of Food Microbiology, 155, 234-240.
https://doi.org/10.1016/j.ijfoodmicro.2012.02.012

[12]   Martinez, F.A., Balciunas, E.M., Converti, A., et al. (2013) Bacteriocin Production by Bifidobacterium spp. A Review. Biotechnology Advances, 31, 482-488.
https://doi.org/10.1016/j.biotechadv.2013.01.010

[13]   Prosser, G.A., Larrouy-Maumus, G. and de Carvalho, L.P. (2014) Metabolomic Strategies for the Identification of New Enzyme Functions and Metabolic Pathways. EMBO Reports, 15, 657-669.
https://doi.org/10.15252/embr.201338283

[14]   Gowda, G.A. and Djukovic, D. (2014) Overview of Mass Spectrometry-Based Metabolomics: Opportunities and Challenges. Methods in Molecular Biology, 1198, 3-12.
https://doi.org/10.1007/978-1-4939-1258-2_1

[15]   Respondek, F., Gerard, P., Bossis, M., et al. (2013) Short-Chain Fructo-Oligosaccharides Modulate Intestinal Microbiota and Metabolic Parameters of Humanized Gnotobiotic Diet Induced Obesity Mice. PLoS One, 8, e71026.
https://doi.org/10.1371/journal.pone.0071026

[16]   Turroni, F., Milani, C., Duranti, S., et al. (2016) Deciphering Bifidobacterial-Mediated Metabolic Interactions and Their Impact on Gut Microbiota by a Multi-Omics Approach. ISME Journal, 10, 1656-1668.
https://doi.org/10.1038/ismej.2015.236

[17]   Sun, Z., Baur, A., Zhurina, D., et al. (2012) Accessing the Inaccessible: Molecular Tools for Bifidobacteria. Applied and Environmental Microbiology, 78, 5035-5042.
https://doi.org/10.1128/AEM.00551-12

[18]   Osswald, A., Sun, Z., Grimm, V., et al. (2015) Three-Dimensional Tumor Spheroids for in Vitro Analysis of Bacteria as Gene Delivery Vectors in Tumor Therapy. Microbial Cell Factories, 14, 199.
https://doi.org/10.1186/s12934-015-0383-5

[19]   Sun, Z., Westermann, C., Yuan, Y., et al. (2014) Characterization of a Gap Promoter for High Level Gene Expression in Bifidobacterium bifidum S17. Bioengineered, 5, 371-377.
https://doi.org/10.4161/bioe.34423

[20]   Osswald, A., Westermann, C., Sun, Z., et al. (2015) A Phytase-Based Reporter System for Identification of Functional Secretion Signals in Bifidobacteria. PLoS ONE, 10, e0128802.
https://doi.org/10.1371/journal.pone.0128802

 
 
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