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 JBM  Vol.6 No.4 , April 2018
CD4 T-Lymphocytes Count in HIV-Toxoplasma gondii Co-Infected Pregnant Women Undergoing a Prevention of Mother-to-Child Transmission Program
Abstract: Toxoplasma gondii (T. gondii) is a parasite responsible of toxoplasmosis, a disease often asymptomatic but with serious consequences in pregnant women and immunocompromised subjects. Objective: This study aimed to investigate the impact of T. gondii infection on CD4+ T lymphocytes count in HIV-infected pregnant women. Methods: This was a cross-sectional study of pregnant women co-infected by HIV and T. gondii. The study was conducted from January to July 2016 at the Prevention of Mother-to-Child Transmission of HIV (PMTCT) sites in the Health District of Lacs in Togo. Diagnosis of HIV was performed by immuno-chromatographic methods with Determine TM HIV-1/2 and immuno-filtration with Tri-Dot HIV-1 and 2 kits. Presence of anti-toxoplasmic IgG and IgM antibodies was established via enzyme immunoassay using ELISA-BIOREX® kit. Flow cytometry was used to count CD4+ T lymphocytes. Results: Our study found that of the 4599 pregnant women, 111 (2.41%) were HIV-positive. Among them, 109 (98.20%) were infected by HIV-1 and 2 (1.98%) by HIV-2. Antibodies against T. gondii were detected in 5.36% (IgM), 25% (IgG) and 3.57% (both IgM and IgG) of HIV 56 infected women. There was no significant difference between CD4 cell count in HIV (+)/T. gondii IgM (-)/IgG (-) infected pregnant women (378.8 ± 222.8 cell//μl) compared to HIV (+)/T. gondii/IgM (+) (457.3 ± 183.3 cell//μl), HIV (+)/T. gondii IgG (+) (419.4 ± 287.3 cell//μl) and HIV (+)/T. gondii IgM/IgG (+) (480.5 ± 252.4 cell/μl). Conclusion: This study showed that intracellular parasite T. gondii did not alter CD4+ T lymphocytes count in HIV/T. gondii co-infected pregnant women.
Cite this paper: Katawa, G. , Kolou, M. , Nadjir, L. , Ataba, E. , Bomboma, G. and Karou, S. (2018) CD4 T-Lymphocytes Count in HIV-Toxoplasma gondii Co-Infected Pregnant Women Undergoing a Prevention of Mother-to-Child Transmission Program. Journal of Biosciences and Medicines, 6, 76-84. doi: 10.4236/jbm.2018.64006.
References

[1]   United Nations Programme on HIV/AIDS (2016) AIDS by the Numbers. Joint United Nations Programme on HIV/AIDS (UNAIDS), Geneva.

[2]   United Nations Programme on HIV/AIDS (2015) HIV and AIDS estimates (2015). United Nations Programme on HIV/AIDS (UNAIDS), Geneva.

[3]   Casalini, C., Signorini, L., Beltrame, A., Matteelli, A. and Carosi, G. (2001) Vertical Trasmission of Human Immunodeficiency Virus (HIV) and Other Sexually Transmitted Infections (STI). Minerva Ginecologica, 53, 177-192.

[4]   Rouzioux, C., Chaix, M.L., Burgard, M. and Mandelbrot, L. (2002) HIV and Pregnancy. Pathologie Biologie, 50, 576-579.
https://doi.org/10.1016/S0369-8114(02)00353-X

[5]   Stascheit, F., Paul, F., Harms, L. and Rosche, B. (2015) Toxoplasma gondii Seropositivity Is Negatively Associated with Multiple Sclerosis. Journal of Neuroimmunology, 285, 119-124.
https://doi.org/10.1016/j.jneuroim.2015.05.011

[6]   Adou-Bryn, K.D., Ouhon, J., Nemer, J., Yapo, C.G. and Assoumou, A. (2004) Serological Survey of Acquired Toxoplasmosis in Woman of Childbearing Age in Yopoug on (Abidjan, Cote d’Ivoire). Bulletin de la Societe de pathologie exotique, 97, 345-348.

[7]   Lindstrom, I., Kaddu-Mulindwa, D.H., Kironde, F. and Lindh, J. (2006) Prevalence of Latent and Reactivated Toxoplasma gondii Parasites in HIV-Patients from Uganda. Acta Tropica, 100, 218-222.
https://doi.org/10.1016/j.actatropica.2006.11.002

[8]   Simpore, J., Pietra, V. and Savadogo, A. (2006) Reduction of Mother-to-Child Transmission of HIV at Saint Camille Medical Centre in Burkina Faso. Journal of Medical Virology, 78, 148-152.
https://doi.org/10.1002/jmv.20521

[9]   Onadeko, M.O., Joynson, D.H., Payne, R.A. and Francis, J. (1996) The Prevalence of Toxoplasma Antibodies in Pregnant Nigerian Women and the Occurrence of Stillbirth and Congenital Malformation. African Journal of Medicine and Medical Sciences, 25, 331-334.

[10]   Bouratbine, A.E., Siala, M.K., Chahed, K.A. and Ben-Ismail, R. (2001) Seroepidemiologic Profile of Toxoplasmosis in Northern Tunisia. Parasite, 8, 61-66.
https://doi.org/10.1051/parasite/2001081061

[11]   Elnahas, A., Gerais, A.S., Elbashir, M.I., Eldien, E.S. and Adam, I. (2003) Toxoplasmosis in Pregnant Sudanese Women. Saudi Medical Journal, 24, 868-870.

[12]   Faye, O., Leye, A., Dieng, Y., Richard-Lenoble, D. and Diallo, S. (1998) Toxoplasmosis in Dakar. Seroepidemiologic Sampling of 353 Women of Reproductive Age. Bulletin de la Societe de pathologie exotique, 91, 249-250.

[13]   Rodier, M.H., Berthonneau, J., Bourgoin, A., Giraudeau, G. and Agius, G. (1995) Seroprevalences of Toxoplasma, Malaria, Rubella, Cytomegalovirus, HIV and Treponemal Infections among Pregnant Women in Cotonou, Republic of Benin. Acta Tropica, 59, 271-277.
https://doi.org/10.1016/0001-706X(95)00087-U

[14]   Ouermi, D., Simpore, J., Belem, A.M., Sanou, D.S., Karou, D.S., Ilboudo, D., et al. (2009) Co-Infection of Toxoplasma gondii with HBV in HIV-Infected and Uninfected Pregnant Women in Burkina Faso. Pakistan Journal of Biological Sciences, 12, 1188-1193.
https://doi.org/10.3923/pjbs.2009.1188.1193

[15]   Singh, R.K. and Patra, S. (2015) What Factors Are Responsible for Higher Prevalence of HIV Infection among Urban Women than Rural Women in Tanzania? Ethiopian Journal of Health Sciences, 25, 321-328.
https://doi.org/10.4314/ejhs.v25i4.5

[16]   Montoya, J.G. (2002) Laboratory Diagnosis of Toxoplasma gondii Infection and Toxoplasmosis. The Journal of Infectious Diseases, 185, S73-S82.
https://doi.org/10.1086/338827

[17]   Fricker-Hidalgo, H., Cimon, B., Chemla, C., Darde, M.L., Delhaes, L., L’Ollivier, C., et al. (2013) Toxoplasma Seroconversion with Negative or Transient Immunoglobulin M in Pregnant Women: Myth or Reality? A French Multicenter Retrospective Study. Journal of Clinical Microbiology, 51, 2103-2111.
https://doi.org/10.1128/JCM.00169-13

[18]   Zumla, A., Savva, D., Wheeler, R.B., Hira, S.K. and Luo, N.P. (1991) Toxo-plasma Serology in Zambian and Uganda Patients Infected with the Human Immunodeficiency Virus. Transactions of the Royal Society of Tropical Medicine and Hygiene, 85, 227-229.
https://doi.org/10.1016/0035-9203(91)90034-V

[19]   Maiga, I., Kiemtore, P. and Tounkara, A. (2001) Prevalence of Antitoxoplasma Antibodies in Patients with Acquired Immunodeficiency Syndrome and Blood Donors in Bamako. Bulletin de la Societe de Pathologie Exotique, 94, 268-270.

[20]   Apetse, K., Assogba, K., Kevi, K., Balogou, A.A.K., Pitche, P. and Grunitzky, E. (2015) Opportunistic Infections of HIV/AIDS in a Neurological Unit in Togo. African Journal of Neurological Sciences, 33, 34-40.

[21]   Wilking, H., Thamm, M., Stark, K., Aebischer, T. and Seeber, F. (2016) Prevalence, Incidence Estimations, and Risk Factors of Toxoplasma gondii Infection in Germany: A Representative, Cross-Sectional, Serological Study. Scientific Reports, 6, Article No. 22551.
https://doi.org/10.1038/srep22551

[22]   Dokekias, A.E., Galiba, F.O. and Bokilo, A.D. (2008) Evaluation of Antiretroviral Therapy in HIV-Infected Adults in the Derpartement of Haematology, University Hospital of Brazaville, Congo. Bulletin de la Societe de pathologie exotique et de ses filiales, 101, 109-112.

[23]   Kaplan, J.E., Hu, D.J., Holmes, K.K., Jaffe, H.W., Masur, H. and De Cock, K.M. (1996) Preventing Opportunistic Infections in Human Immunodeficiency Virus-Infected Persons: Implications for the Developing World. The American Journal of Tropical Medicine and Hygiene, 55, 1-11.
https://doi.org/10.4269/ajtmh.1996.55.1.TM0550010001

[24]   Djibril, M., Ouedraogo, S., Balaka, A., Tchamdja, T., Djagadou, K. and Agbetra, A. (2015) Profile of People Living with HIV in Intensive Medical Care in Togo; Epidemiological and Evolutionary Aspects. Journal of Hematology and Thromboembolic Diseases, 3, 201.
https://doi.org/10.4172/2329-8790.1000201

 
 
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