ABC  Vol.8 No.2 , April 2018
Growth Retardation at Different Stages of Bean Seedlings Developed from Seeds Exposed to Synchrotron X-Ray Beam
Abstract: Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchroton X-Ray Beam (BL-07) at RRCAT, Indore at various doses in the range of 0.5 - 10 Gy were used to raise the seedling and the growth status at different stages was evaluated. Prior to germination, in the seeds soaked for 24 hours, the water regain remained unaffected by seed irradiation at 1 - 10 Gy doses, while the acid phosphatase activity was significantly reduced. Strong correlation (R2 = 0.685) between irradiation dose and enzyme activity also resulted. Analysing seed irradiation effect on seed development up to 4 days, % germination, germination index (GI), seedling wt, and seedling vigour were non significantly decreased at 5.0 Gy dose. The overall growth of 10 days old seedlings raised from irradiated seeds was substantially reduced at irradiation doses of 2 and 5 Gy exerting strong -ve correlation. Also % germination and seed vigour index (SVI) were prominently decreased due to seed irradiation. The nitrogen status of the seedlings, reflected by nitrate reductase activity (NRA) was significantly reduced in response to irradiation exerting strong correlation. The results demonstrate decreased phosphate mobilization in soaked seeds, time dependent decreased growth being more substantial with longer duration and reduced nitrate assimilation due to seed irradiation with Synchroton X-Ray Beam.
Cite this paper: Dhamgaye, S. , Dhamgaye, V. and Gadre, R. (2018) Growth Retardation at Different Stages of Bean Seedlings Developed from Seeds Exposed to Synchrotron X-Ray Beam. Advances in Biological Chemistry, 8, 29-35. doi: 10.4236/abc.2018.82003.

[1]   Sidorov, V.P. (1994) Cytogenic Effect in Pinus sylvestris Needle Cells as a Result of the Chernobyl Accident Radiation Biology. Radioecology, 34, 847-851.

[2]   Prazeres, J.N., Ferreira, C.V. and Aoyama, H. (2004) Acid Phosphatase Activities during the Germination of Glycine Max Seeds. Plant Physiology and Biochemistry, 42, 15-20.

[3]   Serenna, R., Simonin, V., Silva-Neto, M.A.C. and Fialho, E. (2006) Induction of Acid Phosphatase Activity during Germination of Maize (Zea mays) Seeds. Plant Physiology and Biochemistry, 44, 467-473.

[4]   Srivastava, H.S. (1980) Regulation of Nitrate Reductase in Higher Plants. Phytochemistry, 19, 725-733.

[5]   Rockel, P., Strube, F., Rockel, A., Wildt, J. and Kaiser, W.M. (2002) Regulation of Nitric Oxide Production by Plant Nitrate Reductase in vivo and in vitro. Journal of Experimental Botany, 53, 103-110.

[6]   Arena, C., De Micco, V. and De Maio, A. (2014) Growth Alteration and Leaf Biochemical Responses in Phaseolus vulgaris Exposed to Different Doses of Ionising Radiation. Plant Biology, 16, 194-202.

[7]   Dhamgaye, V., Lodha, G., Gowri Sankar, B. and Kant, C. (2014) Beamline BL-07 at Indus-2: A Facility for Microfabrication Research. Journal of Synchrotron Radiation, 21, 259-263.

[8]   Srivastava, H.S. (1975) Distribution of Nitrate Reductase in Bean Seedlings. Plant Cell Physiology, 16, 995-999.

[9]   Mortzavi, S.M.J., Mehdi-Pour, L.A., Tanavardi, S., Mohammadi, S., Kazempour, S., Fatehi, S., Behnejad, B. and Mozdarani, H. (2006) The Biopositive Effects of Diagnostic Doses of X-Rays on Growth of Phaseolus vulgaris Plant : A Possibility of New Physical Fertilizers. Asian Journal of Experimental Sciences, 20, 27-33.

[10]   Al-Enezi, N.A., Al-Bahrany, A.M. and Al-Khayri, J.M. (2012) Effect of X-Irradiation on Date Palm Seed Germination and Seedling Growth. Emirates Journal of Food and Agriculture, 24, 415-424.

[11]   Campbell, W.H. (2001) Structure and Function of Eukaryotic NAD(P)H: Nitrate Reductase. Cell Molecular Life Science, 53, 194-204.

[12]   Dhamgaye, S. and Gadre, R. (2015) Salinity Stress Effects on Growth and Nitrate Assimilation in Bean Seedlings Likely to Be Mediated via Nitric Oxide. Journal of Stress Physiology and Biochemistry, 11, 137-146.